Induced titration device for cell in-vitro culture
By designing an automated titration device, the problem of frequent manual monitoring of drug titration in in vitro cell culture was solved, realizing automated titration and temperature control, adapting to the use of various culture dishes, and improving experimental efficiency and applicability.
Patent Information
- Application Number
- CN202520144043.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-22
- Publication Date
- 2026-01-23
- Estimated Expiration
- 2035-01-22
AI Technical Summary
In existing technologies, continuous and long-term titration of reagents is required during cell culture in vitro. The lack of automated titration devices leads to frequent manual monitoring, which affects experimental efficiency.
An induced titration device was designed, comprising a disc base, a vertical rod, a fixing plate, a cylindrical sleeve, a turntable, and a servo motor. Automatic titration is achieved through a cylinder and a syringe, and the turntable rotates automatically using a servo motor and gear structure to adapt to different petri dish heights. It is also equipped with a heat lamp and a cycle timer to achieve automated titration.
It enables automated titration during long-term incubation processes, reduces the frequency of manual monitoring, improves experimental efficiency, adapts to the titration needs of various solutions, maintains suitable temperatures, and meets the usage requirements of various petri dishes.
Smart Images
Figure CN223823608U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological cell culture technology, and in particular to an induction titration device for in vitro cell culture. Background Technology
[0002] Cell culture involves growing and multiplying cells in vitro, allowing for observation, monitoring, and data recording of the morphology and structure of living cells. Research on in vitro cell culture using biomaterials with different properties, functions, and morphologies has a long history. In recent years, with the interdisciplinary development of new materials, biology, medicine, and engineering, the application of different biomaterials in tissue engineering has gradually attracted attention. For example, Professor Yang Guang's team at Huazhong University of Science and Technology used a conductive biocompatible material for in vitro fibroblast culture, while Jiang Jian's team at the Second Military Medical University has repeatedly explored the effects of electret membrane materials on skin cells. Although the field of tissue engineering is developing rapidly, and many materials are used for in vitro cell culture with the help of 3D printing and other technologies, the main approach involves placing pre-processed scaffold materials into culture dishes for co-culture, "seeding" cells or tissues onto the scaffold material for performance evaluation or differentiation studies. The above method is feasible for most materials that can be directly immersed in liquid, but it has limitations for some pre-formed membrane materials, charged materials, materials that cannot be directly in contact with liquid and need to be encapsulated, and materials that cannot be well fixed to the bottom of the culture dish. Therefore, the technology of in vitro cell culture has emerged.
[0003] The existing technical solutions have the following drawbacks: However, during the in vitro culture of cells, it is necessary to continuously titrate the cleavage agent in the culture dish / test tube for a long time, including but not limited to nutrient agents, inducing agents, etc. Therefore, there is a need for an automatic titration device that can replace manual titration. Utility Model Content
[0004] The purpose of this invention is to provide an induction titration device for in vitro cell culture to solve the problems existing in the prior art.
[0005] The above-mentioned technical objective of this utility model is achieved through the following technical solution:
[0006] An induction titration device for in vitro cell culture includes a disc base. A vertical rod is inserted at the center of the top of the disc base. A first bearing is fitted at the top of the vertical rod. A fixing plate is fitted on the first bearing. A plurality of cylindrical sleeves are axially and evenly inserted into the top of the fixing plate. The top and bottom of the cylindrical sleeves are completely open. The bottom of the cylindrical sleeves passes through and extends to the bottom of the fixing plate. A retaining ring is provided at the inner bottom of the cylindrical sleeves. A threaded surface is provided at the inner top of the cylindrical sleeves. A cover plate is screwed to the top of the cylindrical sleeves. A cylinder is provided at the bottom of the cover plate. A syringe is detachably installed inside the sleeves. A needle is provided at the bottom of the syringe. The needle passes through and extends to the bottom of the cylindrical sleeve. An end face bearing is concentrically arranged on the top of the disc base. A turntable is fixedly installed on the top of the end face bearing. The turntable is axially rotatable. A plurality of receiving grooves are axially and evenly arranged on the top of the turntable.
[0007] By adopting the above technical solution, the multiple accommodating slots on the top of the turntable can accommodate multiple test tubes or culture dishes. Through the cylindrical sleeve structure set on the fixed plate at the top of the vertical rod, under the intervention of the control program, the cylinder push rod can be extended at regular intervals to squeeze the syringe to titrate the nutrient solution or drug mixture inside the test tube / culture dish, thereby realizing automatic titration. This meets the requirements for drug titration work for long-term culture, avoids the problem of needing manual monitoring 24 hours a day, and greatly facilitates long-term tracking biological experiments.
[0008] In a further embodiment, a servo motor is provided at the top of the disc base, a gear ring is fitted on the turntable, and a first gear is fitted on the shaft of the servo motor, the first gear meshing with the gear ring.
[0009] By adopting the above technical solution, since no additional drive module is set on the fixed plate at the top of the vertical rod, automatic rotation cannot be achieved. However, the automatic rotation of the turntable can be achieved through the cooperation of the servo motor, the first gear and the gear ring. This setting allows different solutions to be set in advance in the syringe inside the cylindrical sleeve, so as to achieve titration of multiple solutions inside the test tube / culture dish, making the application range of this device wider.
[0010] In a further embodiment, the vertical rod includes an outer sleeve and an inner circular rod. The outer sleeve is fitted onto the inner circular rod, and the outer sleeve can slide relative to the inner circular rod. A servo electric cylinder is provided at the bottom end of the inner circular rod, and the servo electric cylinder is used to drive the inner circular rod to slide up and down.
[0011] By adopting the above technical solution, because the height of the petri dishes / test tubes is different, in order to avoid the problem of splashing of medicine in the petri dishes / test tubes due to excessive titration height during the titration process, the vertical rod can be automatically adjusted up and down to ensure that the medicine is injected into the petri dish / test tube at the appropriate height for each titration.
[0012] In a further embodiment, a plurality of heat-insulating lamps are evenly arranged around the bottom circumference of the fixing plate.
[0013] By adopting the above technical solution, a certain temperature needs to be maintained in some cultivation processes, and heat lamps can effectively achieve this function.
[0014] In a further embodiment, the top of the disk is detachably provided with a cover, which is used to cover the fixing plate, the vertical rod, the cylindrical sleeve, and the turntable.
[0015] By adopting the above technical solution, the heat preservation effect of the heat lamp is increased.
[0016] In a further embodiment, a cycle timer is provided on the top of the fixing plate.
[0017] In summary, this utility model has the following beneficial effects:
[0018] 1. Multiple test tubes or petri dishes can be accommodated through the multiple accommodating slots on the top of the turntable. The cylindrical sleeve structure set on the fixed plate at the top of the vertical rod can realize automatic titration by extending the cylinder push rod at timed intervals under the intervention of the control program to squeeze the syringe to titrate the nutrient solution or drug mixture inside the test tube / petri dish. This meets the needs of drug titration for long-term culture and avoids the problem of needing manual monitoring 24 hours a day, which greatly facilitates long-term tracking biological experiments. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0020] Figure 2 This is a schematic diagram illustrating the internal structure of the cylindrical sleeve of this utility model.
[0021] In the diagram, 1. Circular base; 2. Vertical rod; 21. Outer rod; 22. Inner rod; 3. First bearing; 4. Fixing plate; 5. Cylindrical sleeve; 6. Snap ring; 7. Cover plate; 8. Syringe; 9. Turntable; 10. Servo motor; 11. Heat preservation lamp. Detailed Implementation
[0022] The present invention will be further described in detail below with reference to the accompanying drawings.
[0023] Identical parts are indicated by the same reference numerals. It should be noted that the terms "front," "rear," "left," "right," "upper," and "lower" used in the following description refer to the attached figures. Figure 1 In this specification, the terms "bottom surface" and "top surface," "inner" and "outer" refer to the direction toward or away from the geometry of a specific component. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this specification, "a plurality of" means two or more, unless otherwise explicitly and specifically defined by the direction of the center.
[0024] Example 1:
[0025] like Figures 1-2As shown, an induction titration device for in vitro cell culture includes a disc base 1. A vertical rod 2 is inserted at the top center of the disc base 1. A first bearing 3 is sleeved on the top of the vertical rod 2. A fixing plate 4 is sleeved on the first bearing 3. A plurality of cylindrical sleeves 5 are axially and evenly inserted into the top of the fixing plate 4. The top and bottom of the cylindrical sleeves 5 are completely open. The bottom of the cylindrical sleeves 5 passes through and extends to the bottom of the fixing plate 4. A retaining ring 6 is provided at the inner bottom of the cylindrical sleeves 5. A threaded surface is provided at the inner top of the cylindrical sleeves 5. A cover plate 7 is screwed to the top of the cylindrical sleeves 5. A cylinder is provided at the bottom of the cover plate 7. A syringe 8 is detachably installed inside the sleeve. A piston is provided inside the syringe 8. The cylinder's push rod... The cylinder extends when needed, pressing down on the piston inside the syringe to eject the liquid medicine. A needle is located at the bottom of the syringe 8, passing through and extending to the bottom of the cylindrical sleeve 5. An end-face bearing is concentrically mounted on the top of the disc base 1, and a turntable 9 is fixedly installed on the top of the end-face bearing. The turntable 9 can rotate axially, and multiple test tubes or culture dishes can be accommodated through multiple receiving slots on the top of the turntable. The cylindrical sleeve structure on the fixed plate at the top of the vertical rod allows for timed extension of the cylinder rod under control, squeezing the syringe to titrate the nutrient solution or medicine mixture inside the test tube / culture dish, thus achieving automatic titration and meeting the needs of long-term... The time-based titration of the drug solution avoids the need for 24-hour manual monitoring, greatly facilitating long-term, tracking biological experiments. The top of the turntable 9 has multiple axially evenly distributed receiving slots, and the top of the disc base 1 is equipped with a servo motor 10. A gear ring is fitted onto the turntable 9, and a first gear is fitted onto the shaft of the servo motor 10, meshing with the gear ring. Because there is no additional drive module for the fixed plate at the top of the vertical rod, automatic rotation is not possible. However, the automatic rotation of the turntable can be achieved through the interaction of the servo motor, the first gear, and the gear ring. This setup allows for the pre-preparation of different solutions in the syringe inside the cylindrical sleeve, enabling the titration of the test... The ability to titrate multiple solutions inside the tube / culture dish broadens the applicability of this device. The vertical rod 2 includes an outer rod 21 and an inner circular rod 22. The outer rod 21 is fitted onto the inner circular rod 22, and the outer tube can slide relative to the inner circular rod 22. A servo electric cylinder is installed at the bottom of the inner circular rod 22 to drive the inner circular rod 22 to slide up and down. Multiple heat lamps 11 are evenly arranged around the bottom of the fixing plate 4. A cover is detachably installed on the top of the disc to cover the fixing plate 4, the vertical rod 2, the cylindrical sleeve 5, and the turntable 9. A cycle timer is installed on the top of the fixing plate 4 to assist in recording the titration time interval, which helps the operator control the titration time better.
[0026] In the embodiments disclosed in this utility model, the terms "installation," "connection," "linking," and "fixing" should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; "linking" can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments disclosed in this utility model according to the specific circumstances.
[0027] This specific embodiment is merely an explanation of the present utility model and is not intended to limit the present utility model. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but as long as they are within the scope of the claims of the present utility model, they are protected by patent law.
Claims
1. An induction titration device for in vitro cell culture, characterized in that: The system includes a disc base (1), a vertical rod (2) inserted at the top center of the disc base (1), a first bearing (3) sleeved on the top of the vertical rod (2), a fixing plate (4) sleeved on the first bearing (3), and a plurality of cylindrical sleeves (5) evenly inserted axially on the top of the fixing plate (4). The top and bottom of the cylindrical sleeves (5) are completely open, and the bottom of the cylindrical sleeves (5) passes through and extends to the bottom of the fixing plate (4). A retaining ring (6) is provided at the inner bottom of the cylindrical sleeves (5). The inner top of the cylindrical sleeve (5) is provided with a threaded surface. The top of the cylindrical sleeve (5) is screwed with a cover plate (7). The bottom of the cover plate (7) is provided with a cylinder. The sleeve is detachably provided with a syringe (8). The bottom of the syringe (8) is provided with a needle. The needle passes through and extends to the bottom of the cylindrical sleeve (5). The top of the disc base (1) is concentrically provided with an end face bearing. The top of the end face bearing is fixedly installed with a turntable (9). The turntable (9) can rotate axially. The top of the turntable (9) is axially uniformly provided with multiple receiving grooves.
2. The induction titration device for in vitro cell culture according to claim 1, characterized in that: A servo motor (10) is provided at the top of the disc base (1), a gear ring is fitted on the turntable (9), and a first gear is fitted on the shaft of the servo motor (10), which meshes with the gear ring.
3. The induction titration device for in vitro cell culture according to claim 1, characterized in that: The vertical rod (2) includes an outer rod (21) and an inner rod (22). The outer rod (21) is sleeved on the inner rod (22), and the outer rod can slide relative to the inner rod (22). A servo electric cylinder is provided at the bottom end of the inner rod (22), and the servo electric cylinder is used to drive the inner rod (22) to slide up and down.
4. The induction titration device for in vitro cell culture according to claim 1, characterized in that: Multiple heat-insulating lamps (11) are evenly arranged around the bottom circumference of the fixing plate (4).
5. The induction titration device for in vitro cell culture according to claim 1, characterized in that: The top of the disc is detachably provided with a cover, which is used to cover the fixing plate (4), the vertical rod (2), the cylindrical sleeve (5), and the turntable (9).
6. The induction titration device for in vitro cell culture according to claim 1, characterized in that: A cycle timer is provided on the top of the fixing plate (4).