Optical focusing sampling device

Abstract

The utility model discloses an optical focusing sampling device, and relates to the technical field of image acquisition, an image sampling component is assembled on a reference unit, a driving module can drive a cantilever structure to move along the axis direction of a focusing lens, the cantilever structure is provided with a moving guide groove matched with a shading sleeve, and the shading sleeve is provided with a light-emitting diode (LED) light-emitting diode (LED) light-emitting diode (LED) light-emitting diode (LED) light-emitting diode (LED) light-emitting diode. The movable guide groove is annularly arranged outside the focusing lens; one end of the shading sleeve is connected to the reference unit, the other end of the shading sleeve is matched with the moving guide groove, and when the cantilever structure moves, at least part of the shading sleeve extends into the moving guide groove. According to the optical focusing sampling device provided by the utility model, the focusing position can be quickly and accurately adjusted through the accurate movement of the focusing lens in the axial direction, so that the optical focusing sampling device is suitable for consumables with different fluxes and heights, and the accuracy and the efficiency of fluorescent image acquisition are improved; in addition, it can be ensured that the shading sleeve can prevent external light from entering the focusing lens in the focusing process, and the image purity and the detection accuracy are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to image acquisition technical field, concretely relates to an optical focusing sampling device. BACKGROUND

[0002] In the field of PCR detection technology, PCR instrument (polymerase chain reaction instrument) is widely used for qualitative and quantitative analysis of the sample to be measured. The usual analysis method is to emit excitation light by light source, and the excitation light irradiates the sample to be measured which has been processed, so as to excite fluorescence from the sample, that is, emission light. The excited fluorescence is collected by the detection instrument, and then the sample to be measured is analyzed according to the collection result. This process has very high precision requirements for fluorescence image, and any inaccuracy of focal position will cause the collected fluorescence image to be blurred, thereby affecting the accuracy of detection.

[0003] With the development of PCR detection technology, higher requirements are put forward for the flux and versatility of PCR instrument. When different flux and height consumables are used, the optimal focal position of fluorescence collection will also change. The existing focusing technology mostly adopts manual focusing mode, which has the following problems: manual focusing needs users to frequently adjust the focal position, especially when replacing consumables, the focusing needs to be recalibrated, which not only increases the complexity of operation, but also reduces the detection efficiency; the calibration process after replacing consumables each time is time-consuming and laborious, especially when the flux is large, multiple sample positions need to be calibrated, which greatly increases the labor intensity of users; when the height change of consumables is small, manual focusing is difficult to achieve accurate focal position, resulting in poor image collection precision and affecting the accuracy of detection results. In the process of manual focusing and semi-automatic focusing, external stray light is easy to enter the focusing lens, causing the fluorescence image to be blurred and polluted, further affecting the accuracy of detection. Especially in the application of multi-module and multi-specification consumables, the interference problem of external light is more serious, because different specifications of consumables may cause the distance between the focusing lens and the sample to change, thereby introducing more stray light. UTILITY MODEL CONTENTS

[0004] The utility model aims at: aiming at the above-mentioned problems, the utility model provides a kind of optical focusing sampling device, through the accurate movement of focusing lens in the axial direction, can quickly, accurately adjust focusing position, adapt to different flux and height consumables, improve the precision and efficiency of fluorescence image collection;In addition, by moving guide groove and light shielding sleeve matching, ensure that light shielding sleeve can avoid external light from entering focusing lens during focusing process, improve the purity of image and the accuracy of detection.

[0005] The technical scheme adopted by the utility model is as follows:

[0006] An optical focusing sampling device comprises a reference unit, a sampling unit, an adaptive adjusting unit and a light shielding sleeve, the sampling unit comprises an image sampling component and a focusing lens, the adaptive adjusting unit comprises a driving module and a cantilever structure; the image sampling component is assembled on the reference unit, the focusing lens is coaxially arranged with the image sampling component, the focusing lens is assembled on the cantilever structure, the driving module can drive the cantilever structure to move along the axis direction of the focusing lens, the cantilever structure is provided with a moving guide groove matched with the light shielding sleeve, and the moving guide groove is annularly arranged outside the focusing lens; one end of the light shielding sleeve is connected to the reference unit, and the other end is matched with the moving guide groove; when the cantilever structure moves, the light shielding sleeve at least partially extends into the moving guide groove.

[0007] Further, the cantilever structure is provided with an assembly groove, the assembly groove is provided with a lens support block for limiting the focusing lens, the bottom of the assembly groove is provided with a through hole matched with the focusing lens, and the moving guide groove is annularly arranged outside the lens support block.

[0008] Further, the cantilever structure is provided with an annular protrusion extending towards the reference unit, and the moving guide groove is arranged at the annular protrusion.

[0009] Further, the cross section of the focusing lens is circular, the light shielding sleeve is a cylindrical sleeve, and the moving guide groove is a circular ring groove matched with the light shielding sleeve.

[0010] Further, the driving module comprises a driving device, a transmission device and a focusing connecting block, the driving device can drive the focusing connecting block to move along the axis direction of the focusing lens, one end of the cantilever structure is connected to the focusing connecting block, and the other end is assembled with the focusing lens.

[0011] Further, the reference unit is provided with a fixed plate, the fixed plate is provided with a guide rail parallel to the axis direction of the focusing lens, one side of the focusing connecting block is provided with the cantilever structure, and the other side is matched with the guide rail.

[0012] Further, the fixed plate and the reference unit cooperatively form an L-shaped structure.

[0013] Further, the transmission device is a lead screw, the driving device is a rotary motor, the driving device can act on the lead screw and drive the lead screw to rotate, and the focusing connecting block is threadedly connected with the lead screw.

[0014] Further, the device further comprises a loading unit for loading a target sampling object, and the adaptive adjusting unit can drive the focusing lens to move so that the sampling unit is in focus with the target sampling object.

[0015] Further, the image sampling component is signal connected to a control device, the adaptive adjustment unit is signal connected to and controlled by the control device, the image sampling component and the control device can transmit image data, and the control device can control the adaptive adjustment unit according to the received image data.

[0016] Therefore, the image sampling analysis device has the advantages that:

[0017] 1. The image sampling analysis device can quickly and accurately adjust the focusing position by the accurate movement of the focusing lens in the axial direction, adapt to different flux and height of the consumables, and improve the precision and efficiency of the fluorescence image acquisition.

[0018] 2. The light shielding sleeve and the image sampling component are coaxially arranged, and the moving guide groove is matched with the light shielding sleeve, so that the light shielding sleeve can avoid external light from entering the focusing lens during the focusing process, and the purity of the image and the accuracy of the detection are improved.

[0019] 3. The image sampling analysis device improves the flexibility and stability of the focusing lens by the design of the cantilever structure and the lens support block, and ensures the accurate movement of the focusing lens during the horizontal calibration process. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 is a structural schematic view of the image sampling analysis device of the present application;

[0021] Figure 2 is a sectional view of the image sampling analysis device of the present application;

[0022] Figure 3 is an effect diagram without using a light shielding sleeve;

[0023] Figure 4 is an effect diagram using a light shielding sleeve of the present application.

[0024] Marked in the figure: 1-sampling unit, 2-adaptive adjustment unit, 4-reference unit, 5-fixed plate, 6-moving guide groove, 7-light shielding sleeve, 8-lens support block, 11-image sampling component, 12-focusing lens, 21-driving device, 22-screw rod, 23-focusing connecting block, 24-cantilever structure. DETAILED DESCRIPTION

[0025] The present application will be described in detail below with reference to the drawings.

[0026] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application will be further described in detail below with reference to the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application, and are not used to limit the present application.

[0027] Example 1

[0028] An optical focusing sampling device, such as Figures 1-4 As shown, the system includes a reference unit 4, a sampling unit 1, an adaptive adjustment unit 2, and a light-shielding sleeve 7. The sampling unit 1 includes an image sampling component 11 and a focusing lens 12. The adaptive adjustment unit 2 includes a drive module and a cantilever structure 24. The image sampling component 11 is mounted on the reference unit 4. The focusing lens 12 is coaxially arranged with the image sampling component 11 and mounted on the cantilever structure 24. The drive module can drive the cantilever structure 24 to move along the axis of the focusing lens 12. The cantilever structure 24 is provided with a moving guide groove 6 that matches the light-shielding sleeve 7. The moving guide groove 6 is arranged around the focusing lens 12. One end of the light-shielding sleeve 7 is connected to the reference unit 4, and the other end matches the moving guide groove 6. When the cantilever structure 24 moves, the light-shielding sleeve 7 extends at least partially into the moving guide groove 6.

[0029] The drive module includes, but is not limited to, lead screw 22 transmission, gear transmission, etc. Any method that can drive the cantilever structure 24 to move along the axis of the focusing lens 12 should be considered to fall within the protection scope of this utility model.

[0030] The cantilever structure 24 is provided with an assembly groove, and a lens support block 8 for limiting the focusing lens 12 is provided in the assembly groove. The bottom of the assembly groove is provided with a through hole that matches the focusing lens 12. The movable guide groove 6 is arranged around the lens support block 8.

[0031] The cantilever structure 24 is provided with an annular protrusion extending toward the reference unit 4, and the movable guide groove 6 is provided at the annular protrusion.

[0032] The focusing lens 12 has a circular cross-section, the light-shielding sleeve 7 is a cylindrical tube, and the moving guide groove 6 is an annular groove that matches the light-shielding sleeve 7.

[0033] It also includes a loading unit for loading the target sample, wherein the adaptive adjustment unit 2 can move the focusing lens 12 so that the sampling unit 1 focuses on the target sample.

[0034] The image sampling component 11 is connected to the control device, and the adaptive adjustment unit 2 is connected to and controlled by the control device. Image data can be transmitted between the image sampling component 11 and the control device, and the control device can control the adaptive adjustment unit 2 based on the received image data.

[0035] Example 2

[0036] Example 2 is a further improvement on Example 1; further explanation: identical components will not be repeated here, such as... Figures 1-4As shown, the driving module comprises a driving device 21, a transmission device and a focusing connecting block 23, the driving device 21 can drive the focusing connecting block 23 to move along the axis direction of the focusing lens 12, the cantilever structure 24 is connected to the focusing connecting block 23 at one end and is assembled with the focusing lens 12 at the other end.

[0037] The reference unit 4 is provided with a fixed plate 5, the fixed plate 5 is provided with a guide rail parallel to the axis direction of the focusing lens 12, one side of the focusing connecting block 23 is provided with a cantilever structure 24, and the other side is matched with the guide rail.

[0038] The fixed plate 5 and the reference unit 4 cooperatively form an L-shaped structure.

[0039] The transmission device is a lead screw 22, the driving device 21 is a rotary motor, the driving device 21 can act on the lead screw 22 and drive the lead screw 22 to rotate, and the focusing connecting block 23 is threadedly connected with the lead screw 22.

[0040] The principle and implementation mode of the present application are described by using specific embodiments, and the above embodiment is only used to help understand the method and core idea of the present application. It should be pointed out that, for ordinary skilled in the art, without departing from the principle of the present application, the present application can be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the claims of the present application.

[0041] In the description of the present application, it should be pointed out that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly used when the product of the present application is used, and are only used to facilitate the description of the present application and simplify the description, and therefore cannot be understood as indicating or implying that the device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0042] In the description of the present application, it should be pointed out that, unless otherwise specified and limited, the terms "set", "install", "connect", "connect" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be connected inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

Claims

1. An optical focusing and sampling device, characterized in that, The system includes a reference unit, a sampling unit, an adaptive adjustment unit, and a light-shielding sleeve. The sampling unit includes an image sampling component and a focusing lens. The adaptive adjustment unit includes a drive module and a cantilever structure. The image sampling component is mounted on the reference unit, and the focusing lens is coaxially arranged with the image sampling component. The focusing lens is mounted on the cantilever structure. The drive module can drive the cantilever structure to move along the axis of the focusing lens. The cantilever structure is provided with a moving guide groove that matches the light-shielding sleeve, and the moving guide groove is arranged around the focusing lens. One end of the light-shielding sleeve is connected to the reference unit, and the other end matches the moving guide groove. When the cantilever structure moves, the light-shielding sleeve at least partially extends into the moving guide groove.

2. The optical focusing sampling device as described in claim 1, characterized in that, The cantilever structure is provided with an assembly groove, and a lens support block for limiting the focusing lens is provided in the assembly groove. The bottom of the assembly groove is provided with a through hole that matches the focusing lens, and the movable guide groove is arranged around the lens support block.

3. The optical focusing sampling device as described in claim 1, characterized in that, The cantilever structure is provided with an annular protrusion extending toward the reference unit, and the movable guide groove is provided at the annular protrusion.

4. The optical focusing sampling device as described in claim 3, characterized in that, The focusing lens has a circular cross-section, the light-shielding sleeve is a cylindrical tube, and the moving guide groove is an annular groove that matches the light-shielding sleeve.

5. The optical focusing sampling device as described in claim 1, characterized in that, The drive module includes a drive device, a transmission device, and a focusing connection block. The drive device can drive the focusing connection block to move along the axis of the focusing lens. One end of the cantilever structure is connected to the focusing connection block, and the other end is equipped with the focusing lens.

6. The optical focusing sampling device as described in claim 5, characterized in that, The reference unit is provided with a fixing plate, and the fixing plate is provided with a guide rail parallel to the axis of the focusing lens. The focusing connecting block has a cantilever structure on one side and matches the guide rail on the other side.

7. The optical focusing sampling device as described in claim 6, characterized in that, The fixed plate and the reference unit cooperate to form an L-shaped structure.

8. The optical focusing sampling device as described in claim 5, characterized in that, The transmission device is a lead screw, the driving device is a rotary motor, the driving device can act on the lead screw and drive the lead screw to rotate, and the focusing connecting block is threadedly connected to the lead screw.

9. The optical focusing sampling device as described in claim 1, characterized in that, It also includes a loading unit for loading the target sample, wherein the adaptive adjustment unit can move the focusing lens so that the sampling unit focuses on the target sample.

10. The optical focusing sampling device according to any one of claims 1-9, characterized in that, The image sampling component is signal-connected to the control device, and the adaptive adjustment unit is signal-connected to and controlled by the control device. Image data can be transmitted between the image sampling component and the control device, and the control device can control the adaptive adjustment unit based on the received image data.

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