Device for stably labeling plant by isotope

Abstract

The utility model relates to the technical field of agricultural and forestry stable isotope labeling, and discloses a stable isotope plant labeling device which comprises a box body, and a cooling pipe, a liquid injection pipe and a fan are arranged in the box body; the base plate is located below the box body, a groove is formed in the base plate, the box body is installed in the groove, and a reaction bottle is placed on the base plate. According to the device, the box body is high in light transmission, the groove in the base plate is used, and the liquid injection pipe replaces a method for adding a reagent by opening the box door, so that the photosynthesis of plants is improved, the airtightness of the device can be ensured, and the marking effect is enhanced.

Description

Technical Field

[0001] This utility model relates to the field of stable isotope labeling technology in agriculture and forestry, and more specifically, to a device for stable isotope labeling of plants. Background Technology

[0002] Isotope labeling and tracing techniques are powerful tools for studying elemental turnover processes in the gas-plant-soil system, and have been applied to the study of soil carbon, nitrogen, and other elements' sources and turnover kinetics. Stable isotopes are widely used due to their advantages such as being non-radioactive and having safe and reliable labeling capabilities. Therefore, the development of stable isotope techniques has made it possible to quantitatively study elemental turnover processes.

[0003] The core of stable isotope labeling and tracing technology is to reflect the decomposition and transformation processes of stable isotopes in soil by adding them as labeling materials. However, research on the role of microorganisms in the carbon and nitrogen sources and turnover dynamics between plants and soil is mainly limited by the content of stable isotopes in organic materials. Existing devices have poor labeling effects, and in the study of the tracing process, it is impossible to study the function of microorganisms in the carbon and nitrogen turnover processes between plants and soil.

[0004] Given the significant impact of stable isotope content in organic materials on the study of carbon and nitrogen sources and turnover dynamics between plants and soil, there is an urgent need for a stable isotope labeling device for plants that is simple in structure, easy to operate, and has good labeling effect. Utility Model Content

[0005] The purpose of this invention is to provide a device for stable isotope labeling of plants to solve the problems existing in the prior art. It can realize stable isotope labeling of outdoor plants and obtain plants with high stability of isotope labeling.

[0006] To achieve the above objectives, the present invention provides the following solution: The present invention provides a device for labeling plants with stable isotopes, comprising: a box body, wherein a cooling pipe, a liquid injection pipe and a fan are installed inside the box body; a chassis, wherein the chassis is located below the box body, a groove is provided on the chassis, the box body is installed in the groove, and a reaction bottle is placed on the chassis.

[0007] According to the present invention, a device for labeling plants with stable isotopes is provided, wherein the cooling pipe is fixedly installed on the inner wall of the box to reduce the temperature inside the box and ensure that the plant's photosynthesis proceeds normally.

[0008] According to the present invention, a device for labeling plants with stable isotopes is provided, wherein a plurality of injection tubes are provided, and the plurality of injection tubes are disposed on the side wall of the box body. The injection tubes are L-shaped, with one end of the injection tube located outside the box body and the other end located inside the box body.

[0009] According to the present invention, a device for labeling plants with stable isotopes is provided, wherein a plurality of fans are provided, and the plurality of fans are installed inside the box and distributed on the top and sides of the box.

[0010] According to the present invention, a device for labeling plants with stable isotopes is provided, wherein the box is embedded in the chassis through the groove, and the box and the chassis are sealed together through the groove.

[0011] According to the present invention, a device for labeling plants with stable isotopes is provided, wherein a plurality of reaction bottles are provided, the number of reaction bottles being matched with the number of injection tubes, and one end of the injection tube located inside the box corresponding to the reaction bottle.

[0012] The present invention discloses the following technical effects:

[0013] The device features a highly transparent chamber, and the use of grooves on the base and injection tubes instead of opening the chamber door to add reagents not only improves the photosynthesis of the plants but also ensures the airtightness of the device, enhancing the marking effect. The device has a simple structure, is easy to operate, and is highly portable, making it suitable for both greenhouse and woodland conditions. The temperature is controllable, increasing the stability and repeatability of experimental results. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the internal structure of the box in this utility model;

[0017] Figure 3 This is a schematic diagram of the chassis structure in this utility model;

[0018] The components include: 1. Box body; 2. Cooling pipe; 3. Liquid injection pipe; 4. Fan; 5. Chassis; 6. Groove; 7. Reaction flask. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0021] like Figures 1-3 As shown, this utility model provides a device for labeling plants with stable isotopes, comprising: a box 1, in which a cooling pipe 2, a liquid injection pipe 3 and a fan 4 are installed; a base 5, which is located below the box 1, and a groove 6 is provided on the base 5, in which the box 1 is installed, and a reaction bottle 7 is provided on the base 5.

[0022] Box 1 is made of transparent plexiglass, which has high light transmittance and is easy to move. It is cubic in shape and hollow inside and at the bottom.

[0023] Cooling pipes 2 are installed on the inner wall of the box 1 to reduce the temperature inside the box 1 and ensure the normal respiration and photosynthesis of the plants.

[0024] In this embodiment, four injection tubes 3 are provided. The four injection tubes 3 are installed on the side wall of the box 1. The injection tubes 3 have an L-shaped structure, with one end located outside the box 1 and the other end located inside the box 1. The injection tubes 3 facilitate the addition of reagents into the reaction bottle 7, avoiding gas leakage and loss.

[0025] In this embodiment, five fans 4 are provided. The fans 4 are battery-powered and rechargeable. One fan 4 is installed on the top of the housing 1, and the other four fans 4 are distributed around the perimeter of the housing 1. The airflow direction of the fans 4 is downward for the top fan 4 and upward for the four side fans 4, so that the gas inside the housing generates a circulating airflow, thereby making the isotope-labeled gas evenly distributed inside the housing 1.

[0026] The chassis 5 is provided with a groove 6, which is adapted to the side wall of the housing 1. The groove 6 on the chassis 5 allows the housing 1 to be completely embedded in the chassis 5, which can ensure the sealing of the device.

[0027] A reaction bottle 7 is placed on the chassis 5. The number of reaction bottles 7 matches the number of injection tubes 3. In this embodiment, there are 4 reaction bottles 7. All 4 reaction bottles 7 are movable. The reaction bottles 7 correspond to the injection tubes 3. One end of the injection tube 3 is located at the top of the reaction bottle 7 inside the box 1, which facilitates the addition of reagents to the reaction bottle 7 through the injection tube 3.

[0028] The method for using this device to label plants with stable isotopes is as follows: First, arrange the potted plants and reaction bottles 7 in an orderly manner in the base plate 5, and place the reaction bottle 7 containing the reagents below the inlet of the injection tube 3, so that the injection tube is suspended above the inlet of the reaction bottle 7. Turn on the fan 4, and then carefully place the box 1 on the base plate 5, and after the box 1 is completely embedded in the groove 6 in the base plate 5, use a 10mL syringe to add the prepared reagent B to the reaction bottle 7 containing reagent A through the glass injection tube 3. Set the labeling time interval and number of times according to the research purpose and the characteristics of plant growth and metabolism, and adjust the temperature inside the box by controlling the flow rate of cooling water in the cooling tube 2.

[0029] In the description of this utility model, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0030] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Various modifications and improvements made to the technical solutions of the present utility model by those skilled in the art without departing from the spirit of the present utility model should fall within the protection scope defined by the claims of the present utility model.

Claims

1. A device for stable isotope labeling of plants, characterized in that, include: The housing (1) is equipped with a cooling pipe (2), a liquid injection pipe (3) and a fan (4); The chassis (5) is located below the box (1). The chassis (5) has a groove (6) and the box (1) is installed in the groove (6). The reaction bottle (7) is placed on the chassis (5).

2. The device for stable isotope labeling of plants according to claim 1, characterized in that: The cooling pipe (2) is fixedly installed on the inner wall of the box (1) to reduce the temperature inside the box and ensure that the photosynthesis of the plant proceeds normally.

3. The device for stable isotope labeling of plants according to claim 1, characterized in that: The injection tube (3) is provided in several parts, and the injection tube (3) is provided on the side wall of the box (1). The injection tube (3) has an L-shaped structure, with one end of the injection tube (3) located outside the box (1) and the other end located inside the box (1).

4. The device for stable isotope labeling of plants according to claim 1, characterized in that: The fan (4) is provided in a plurality of units, which are installed inside the housing (1) and distributed on the top and sides of the housing (1).

5. The device for stable isotope labeling of plants according to claim 1, characterized in that: The box (1) is embedded in the chassis (5) through the groove (6), and the box (1) and the chassis (5) are sealed together through the groove (6).

6. The device for stable isotope labeling of plants according to claim 3, characterized in that: The reaction flasks (7) are provided in a number that match the number of injection tubes (3). One end of the injection tube (3) located inside the box (1) corresponds to the reaction flasks (7).

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