Immunohistochemical antibody incubation device for pathological section

Abstract

The utility model provides a pathological section immunohistochemical antibody incubation device which comprises a box body, a hollowed-out plate is fixedly installed in the bottom of the box body, a heat preservation frame is installed above the hollowed-out plate and tightly attached to the inner wall of the box body, a plurality of partition plates are fixedly installed in the heat preservation frame, and the partition plates are connected with the box body. The partition plates divide the space in the heat preservation frame into a plurality of spaces for containing slides, a temperature control assembly used for adjusting the temperature is arranged in the bottom of the box body, and a humidity adjusting assembly used for adjusting the humidity environment in the box is arranged in the heat preservation frame. A slide limiting assembly is arranged in the heat preservation frame of the box body and located between the partition plates on the two sides. The temperature control assembly is controlled by the controller to adjust the temperature in the box, so that the incubation temperature is kept in a proper range, then the humidity is adjusted and kept to be proper through the humidity adjusting assembly, a proper incubation environment can be provided all the time, and the temperature and the humidity of the incubation environment in the box can be kept all the time.

Description

Technical Field

[0001] This utility model relates to the field of medical devices, and in particular to a pathological slide immunohistochemical antibody incubation device. Background Technology

[0002] Immunohistochemistry is a technique that uses the basic principles of immunology—antigen-antibody reaction, namely the principle of specific binding between antigens and antibodies—to identify intracellular antigens by making a chromogenic agent containing labeled antibodies appear colored through a chemical reaction. This allows for the localization, qualitative and relative quantitative analysis of these antigens. Current immunohistochemical experiments typically require the use of an incubation box.

[0003] A patent application (CN202122429535.9) filed on October 9, 2021, discloses an intelligent temperature-controlled incubation chamber for immunohistochemistry antibodies. The chamber includes an incubation box body, an externally mounted display screen, an internally mounted horizontal plate, and an internally mounted temperature control mechanism. This mechanism includes a heater, a heat exchange tube, a fan, a circulation pipe, and a perforated plate. The heater is fixedly installed inside the incubation box body, with a heat exchange tube fixedly mounted on top. A fan is fixedly mounted on the right side of the incubation box body, and a circulation pipe is fixedly connected to the left side of the fan. The perforated plate is also fixedly installed inside the incubation box body. This invention uses a heater to conduct hot air through the heat exchange tube to the circulation pipe, which surrounds the incubation box body, rapidly raising the internal temperature. When cooling is needed, the fan introduces cold air into the incubation box body through the circulation pipe, achieving convenient temperature control.

[0004] While the above technical solution can conveniently control the temperature environment inside the box, it requires the heater to be kept on continuously, which consumes a lot of electricity. Moreover, continuous heating will reduce the humidity inside the box, which will affect the incubation environment. Therefore, further improvements are needed. Summary of the Invention

[0005] To address the problems existing in the background art, this utility model proposes a pathological slide immunohistochemical antibody incubation device.

[0006] An immunohistochemical antibody incubation device for pathological slides includes a box body. A lid is hinged to the top of the box body, and a sealing gasket is fixedly installed on the bottom side of the lid. A perforated plate is fixedly installed inside the bottom of the box body. An insulation frame is installed above the perforated plate and close to the inner wall of the box body. Several partitions are fixedly installed inside the insulation frame, dividing the space inside the insulation frame into several spaces for accommodating glass slides. A temperature control component for adjusting temperature is provided inside the bottom of the box body. Ventilation fans are provided on both the left and right sides of the box body. A humidity control component for adjusting the humidity environment inside the box is provided inside the insulation frame. A controller for controlling various components is provided on the front of the box body. A glass slide limiting component is provided inside the insulation frame and between the two side partitions.

[0007] Based on the above, the temperature control component includes a heater fixedly installed on one side of the back of the box, a heating tube fixedly installed on one side of the front of the heater, and the heating tube is located below the hollow plate inside the box. A temperature sensor is installed inside the insulation frame.

[0008] Based on the above, the heating tube has an "S" shaped structure that bends back and forth.

[0009] Based on the above, the humidity regulating component includes two sets of U-shaped tubes fixedly installed inside the side wall of the insulation frame, and a humidity sensor installed inside the insulation frame. Humidifiers are fixedly installed in the middle of the left and right sides of the box. A connecting pipe is connected between the middle of the short frame of the two sets of U-shaped tubes. An air inlet pipe is connected in the middle of the connecting pipe, and the other end of the air inlet pipe is connected to the exhaust port of the humidifier. A guide tube is fixedly installed between the long frame of the two sets of U-shaped tubes, and a nozzle is opened on the side of the guide tube opposite to the space for accommodating the glass slide.

[0010] Based on the above, the conduit is configured to accommodate the glass slide, and the inner wall of the insulation frame is provided with a through hole for moisture to enter the insulation frame through the nozzle.

[0011] Based on the above, the slide limiting assembly includes a hollow block located in the space for accommodating the slide. A fixing seat is fixedly installed on the side of the hollow block near the inner wall of the insulation frame, and the fixing seat is fixedly installed on the inner wall of the insulation frame by bolts. A support is slidably inserted into the interior of the hollow block, and a track wheel is rotatably connected to one end of the support located outside the hollow block. A compression spring is fixedly installed between the support and the fixing seat.

[0012] Based on the above, a support plate is fixedly installed on one side of the support inside the hollow block, and the support plate is slidably connected inside the hollow block.

[0013] This invention has substantial features and advancements compared to existing technologies. Specifically, this invention uses a controller to regulate the temperature inside the box by controlling the temperature control component, so that the incubation temperature is maintained within a suitable range. Then, it uses a humidity control component to regulate the humidity inside the box to maintain a suitable humidity, thereby providing a suitable incubation environment at all times. It has the advantage of being able to maintain the temperature and humidity of the incubation environment inside the box at all times.

[0014] Additionally, by pressing the glass slide downwards along the track wheel, the support slides under pressure into the hollow block, which also compresses the compression spring. The spring's rebound force then causes the track wheel to press the glass slide in the opposite direction to position it. By pinching the glass slide and pulling it upwards, it can be easily removed, making the process convenient and quick. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0016] Figure 2 This is a schematic diagram of the internal structure of the box in this utility model.

[0017] Figure 3 This is a schematic diagram of the temperature control structure of this utility model.

[0018] Figure 4 This is a schematic diagram of the humidity regulation structure of this utility model.

[0019] Figure 5 This is a schematic diagram of the glass slide limiting structure of this utility model.

[0020] Explanation of reference numerals in the attached drawings: 1. Box body; 2. Box lid; 3. Sealing gasket; 4. Insulation frame; 5. Partition; 6. Temperature control component; 7. Ventilation fan; 8. Controller; 9. Glass slide limiting component; 10. Heater; 11. Heating tube; 12. U-shaped tube; 13. Humidifier; 14. Connecting pipe; 15. Air inlet pipe; 16. Conduit; 17. Nozzle; 18. Hollow block; 19. Fixing base; 20. Support; 21. Track wheel; 22. Compression spring. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] like Figures 1-5As shown, an immunohistochemical antibody incubation device for pathological slides includes a box body 1, a lid 2 hinged to the top of the box body 1, a sealing gasket 3 fixedly installed on the bottom side of the lid 2, a perforated plate fixedly installed inside the bottom of the box body 1, an insulation frame 4 installed above the perforated plate and close to the inner wall of the box body 1, several partitions 5 fixedly installed inside the insulation frame 4, the partitions 5 dividing the space inside the insulation frame 4 into several spaces for accommodating glass slides, a temperature control component 6 for adjusting temperature is provided inside the bottom of the box body 1, ventilation fans 7 are provided on both the left and right sides of the box body 1, a humidity control component for adjusting the humidity environment inside the box is provided inside the insulation frame 4, a controller 8 for controlling various components is provided on the front of the box body 1, and a glass slide limiting component 9 is provided inside the insulation frame 4 and located between the two side partitions 5.

[0023] In use, first lift the lid 2 upwards, then press the glass slide carrying the slide down along the partitions 5 on both sides to place the slide between the partitions 5. Then, control the temperature control component 6 through the controller 8 to adjust the temperature inside the box so that the incubation temperature is kept within a suitable range. Then, adjust the humidity inside the box through the humidity control component to maintain a suitable humidity, thus providing a suitable incubation environment at all times.

[0024] Specifically, the temperature control component 6 includes a heater 10 fixedly installed on one side of the back of the box body 1, and a heating tube 11 fixedly installed on one side of the front of the heater 10. The heating tube 11 has an "S" shaped structure that bends back and forth, and the heating tube 11 is located below the hollow plate inside the box body 1. A temperature sensor is installed inside the insulation frame 4. By starting the heater 10, the heating tube 11 will heat up. At the same time, the temperature sensor will detect the incubation temperature inside the box in real time, so that heating can be stopped when the temperature is too high and heating can be started when the temperature is too low, thus maintaining a suitable incubation temperature inside the box.

[0025] Specifically, the humidity control component includes two sets of U-shaped tubes 12 fixedly installed inside the side wall of the insulation frame 4, and a humidity sensor installed inside the insulation frame 4. Humidifiers 13 are fixedly installed in the middle of the left and right sides of the box body 1. A connecting pipe 14 connects the middle of the short frame of the two sets of U-shaped tubes 12, and an air inlet pipe 15 connects to the middle of the connecting pipe 14. The other end of the air inlet pipe 15 leads into the exhaust port of the humidifier 13. A conduit 16 is fixedly installed between the long frame of the two sets of U-shaped tubes 12. The conduit 16 corresponds to the space for accommodating the glass slide. The space is designed with a nozzle 17 on the side of the conduit 16 opposite to the space containing the glass slide. The inner wall of the insulation frame 4 is provided with a through hole for moisture to enter the insulation frame 4 through the nozzle 17. By starting the humidifier 13, moisture can be introduced into the loop tube 12 along the air inlet pipe 15. Finally, it will be sprayed out from the nozzle 17 along the conduit 16 and then enter the insulation frame 4 through the through hole to increase the humidity of the environment near the glass slide. The humidity sensor detects the humidity of the internal environment and can adjust it in real time when the humidity is too high or too low.

[0026] In reality, the slide positioning assembly 9 includes a hollow block 18 located in the space containing the slide. A fixing seat 19 is fixedly installed on the side of the hollow block 18 near the inner wall of the insulation frame 4, and the fixing seat 19 is fixedly installed on the inner wall of the insulation frame 4 by bolts. A support 20 is slidably inserted into the interior of the hollow block 18. A support plate is fixedly installed on the side of the support 20 located inside the hollow block 18, and the support plate is slidably connected inside the hollow block 18. A track wheel 21 is rotatably connected to the end of the support 20 located outside the hollow block 18. A compression spring 22 is fixedly installed between the support 20 and the fixing seat 19. By pressing the slide down along the track wheel 21, the support 20 is pressed and slid into the hollow block 18, which compresses the compression spring 22. The rebound force of the compression spring 22 drives the track wheel 21 to squeeze the slide in the opposite direction to position it. By pinching the slide and pulling it up, the slide can be easily removed, which is convenient and quick.

[0027] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A pathological section immunohistochemical antibody incubation device, characterized in that: The device includes a box body, a lid hinged to the top of the box body, a sealing gasket fixedly installed on the bottom side of the lid, a perforated plate fixedly installed inside the bottom of the box body, an insulation frame installed above the perforated plate and close to the inner wall of the box body, several partitions fixedly installed inside the insulation frame, the partitions dividing the space inside the insulation frame into several spaces for accommodating glass slides, a temperature control component for adjusting temperature is provided inside the bottom of the box body, ventilation fans are provided on both the left and right sides of the box body, a humidity control component for adjusting the humidity environment inside the box is provided inside the insulation frame, a controller for controlling various components is provided on the front of the box body, and a glass slide limiting component is provided inside the insulation frame and between the two side partitions.

2. The immunohistochemical antibody incubation device for pathological sections according to claim 1, characterized in that: The temperature control component includes a heater fixedly installed on one side of the back of the box, a heating tube fixedly installed on one side of the front of the heater, and the heating tube is located below the hollow plate inside the box. A temperature sensor is installed inside the insulation frame.

3. The immunohistochemical antibody incubation device for pathological sections according to claim 2, characterized in that: The heating element has an "S" shaped structure that bends back and forth.

4. The immunohistochemical antibody incubation device for pathological sections according to claim 1, characterized in that: The humidity control assembly includes two sets of U-shaped tubes fixedly installed inside the side wall of the insulation frame, and a humidity sensor installed inside the insulation frame. Humidifiers are fixedly installed in the middle of the left and right sides of the box. A connecting pipe is connected between the middle of the short frame of the two sets of U-shaped tubes. An air inlet pipe is connected in the middle of the connecting pipe, and the other end of the air inlet pipe leads into the exhaust port of the humidifier. A guide tube is fixedly installed between the long frame of the two sets of U-shaped tubes corresponding to the space for accommodating the glass slide, and a nozzle is opened on the side of the guide tube opposite to the space for accommodating the glass slide.

5. The immunohistochemical antibody incubation device for pathological sections according to claim 4, characterized in that: The conduit is positioned to accommodate the glass slide, and the inner wall of the insulation frame is provided with a through hole for moisture to enter the insulation frame, corresponding to the nozzle.

6. The immunohistochemical antibody incubation device for pathological sections according to claim 1, characterized in that: The slide limiting assembly includes a hollow block located in the space for accommodating the slide. A fixing seat is fixedly installed on the side of the hollow block near the inner wall of the insulation frame, and the fixing seat is fixedly installed on the inner wall of the insulation frame by bolts. A support is slidably inserted into the interior of the hollow block, and a track wheel is rotatably connected to one end of the support located outside the hollow block. A compression spring is fixedly installed between the support and the fixing seat.

7. The immunohistochemical antibody incubation device for pathological sections according to claim 6, characterized in that: The support plate is fixedly installed on one side of the hollow block, and the support plate is slidably connected inside the hollow block.

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