Immunodetection kit for drug-induced liver injury markers in blood

Abstract

The utility model provides an immunodetection kit for drug-derived liver injury markers in blood, which relates to the technical field of medical detection and comprises a kit body, a drug mixing cavity is arranged on the left side of the upper portion of the kit body, a drug mixing box is fixedly connected in the drug mixing cavity, a detection display cavity is arranged on the right side of the upper portion of the kit body, and the drug mixing box is fixedly connected in the drug mixing cavity. The liver injury marker immunodetection kit comprises a kit body, a reagent mixing box is arranged in the kit body, a detection display cavity is arranged in the reagent mixing cavity, a rotary stirring pipe is rotationally connected in the reagent mixing box, a sealing plug is fixedly connected to the right end of the rotary stirring pipe, and an inter-cavity connecting hole is formed in a partition plate between the reagent mixing cavity and the detection display cavity in a penetrating mode. The buffer agent is contained in the medicament mixing box, the blood sampling cotton swab is hidden in the kit body, and a plurality of detection tools are combined into a whole, so that the use convenience of the kit can be improved, the tools can be prevented from being dispersed and lost, and the sanitary safety is guaranteed.

Description

Technical Field

[0001] This utility model relates to the field of medical testing technology, and in particular to an immunoassay kit for drug-induced liver injury markers in blood. Background Technology

[0002] Drug-induced liver injury refers to damage to the liver caused by the drug itself or its metabolites during drug use. This damage can lead to abnormal liver function and, in severe cases, even liver failure. An immunoassay kit for drug-induced liver injury biomarkers in blood can diagnose drug-induced liver injury. This kit is based on immunological principles and utilizes the binding properties of specific antibodies to target biomarkers. The blood sample is mixed with a buffer solution and then with the antibodies in the kit. Through a series of chemical reactions, an observable signal (such as color change or fluorescence) is generated, thus determining the presence and concentration of the target biomarker in the sample.

[0003] Traditional immunoassay kits for drug-induced liver injury involve soaking a swab containing blood in a buffer solution to ensure the blood sample is fully fused before injecting the reagent. However, the blood sample does not readily fuse with the buffer solution, resulting in low fusion efficiency. Shaking the buffer solution to accelerate fusion can easily lead to leakage of both the buffer solution and the sample, causing unnecessary problems. Utility Model Content

[0004] This disclosure relates to an immunoassay kit for drug-induced liver injury biomarkers in blood. This kit addresses the problem that traditional immunoassay kits for drug-induced liver injury involve soaking a blood-containing swab in a buffer solution to allow the blood sample to fully fuse with the buffer before injecting the reagent. However, with traditional kits, the blood sample does not readily fuse with the buffer solution, resulting in low fusion efficiency. Shaking the buffer solution to accelerate fusion can easily lead to leakage of the buffer solution and sample, causing unnecessary problems.

[0005] In a first aspect, this disclosure provides an immunoassay kit for drug-induced liver injury markers in blood, specifically comprising: a kit body, a drug mixing chamber located on the upper left side of the kit body, a drug mixing container fixedly connected inside the drug mixing chamber, a detection display chamber located on the upper right side of the kit body, a rotating stirring tube rotatably connected inside the drug mixing container, a sealing plug fixedly connected to the right end of the rotating stirring tube, an inter-cavity connecting hole through a partition plate between the drug mixing chamber and the detection display chamber, a detection reagent outlet located on the right wall of the drug mixing container, and a blood sampling swab located inside the rotating stirring tube.

[0006] In at least some embodiments, the left end of the reagent kit box is provided with a stirring tube insertion port, which is tightly connected to the rotating stirring tube.

[0007] In at least some embodiments, a sample inlet is provided at the top of the drug mixing box, a cap is tightly fastened to the upper end of the sample inlet, a buffer solution is contained inside the drug mixing box, and a mixing box insertion port is provided on the left wall of the drug mixing box.

[0008] In at least some embodiments, the mixing box inlet is tightly connected to the rotating stirring tube, and the mixing box inlet and the stirring tube inlet are coaxial.

[0009] In at least some embodiments, a detection agent outlet is provided on the right wall of the drug mixing box. The detection agent outlet is a conical hole and is coaxial with the cavity connecting hole.

[0010] In at least some embodiments, four stirring blades are fixedly connected to the outer wall of the rotating stirring tube, and a stirring handle is fixedly connected to the left end of the rotating stirring tube. A connecting screw hole is opened horizontally through the center of the stirring handle.

[0011] In at least some embodiments, the right end of the sealing plug is provided with a sealing cone surface, which is in close contact with the outlet of the detection agent. A sealing insert is fixedly connected to the right end of the sealing cone surface, and the sealing insert is tightly inserted into the cavity connecting hole.

[0012] In at least some embodiments, the left end of the blood sampling swab is fixedly connected to a swab spiral plug, the swab spiral plug is spirally connected to a docking screw hole, and the left end of the swab spiral plug is fixedly connected to a swab handle.

[0013] This invention provides an immunoassay kit for detecting drug-induced liver injury biomarkers in blood, which has the following beneficial effects:

[0014] In this utility model, the immunoassay kit for liver injury markers integrates the reagent mixing box with the kit body. The reagent mixing box contains a buffer, and the blood sampling swab is hidden inside the kit body. By combining multiple testing instruments into one unit, the kit can improve the ease of use, avoid the dispersion and loss of tools, and ensure hygiene and safety.

[0015] In addition, a rotating stirring tube is installed inside the reagent mixing box. After placing a blood sampling swab containing the blood sample into the reagent mixing box, rotating the stirring tube causes the blades to rotate, agitating the buffer solution and swab inside the reagent mixing box. This helps the blood sample in the swab quickly integrate into the buffer solution, improving the fusion efficiency of the blood sample and buffer solution, further simplifying the detection procedure. A sealing plug is also provided. By pulling the rotating stirring tube, the sealing plug can be removed, connecting the reagent mixing box to the detection reagent inside the detection display cavity. This allows the buffer solution containing the blood sample inside the reagent mixing box to enter the detection reagent and react. The operation is convenient, eliminating the need for secondary sample pouring and preventing sample spillage. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings of the embodiments will be briefly described below.

[0017] The accompanying drawings described below are only related to some embodiments of the present invention and are not intended to limit the scope of the present invention.

[0018] In the attached diagram:

[0019] Figure 1 A schematic diagram of the overall structure of this application is shown;

[0020] Figure 2 A schematic diagram of the internal structure of the drug mixing box of this application is shown;

[0021] Figure 3 This paper shows a schematic diagram of the internal structure of the reagent kit box of this application;

[0022] Figure 4 This paper shows a schematic diagram of the structure in its disassembled state.

[0023] Figure 5 This diagram illustrates the structure of the reagent kit body and the drug mixing box when separated.

[0024] Figure 6 A schematic diagram of the internal structure of the rotating stirring tube of this application is shown.

[0025] List of reference numerals

[0026] 1. Reagent kit body; 101. Reagent mixing chamber; 102. Detection display chamber; 103. Stirring tube inlet; 104. Inter-chamber connecting hole; 2. Reagent mixing box; 201. Sample inlet; 202. Inlet cap; 203. Mixing box inlet; 204. Detector outlet; 3. Rotary stirring tube; 301. Stirring tube blade; 302. Stirring tube handle; 303. Connecting screw hole; 4. Sealing plug; 401. Sealing cone surface; 402. Sealing insert; 5. Blood sampling swab; 501. Swab spiral plug; 502. Swab handle. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the described embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0028] Example 1: Please refer to Figures 1 to 6 :

[0029] This invention proposes an immunoassay kit for drug-induced liver injury markers in blood, comprising: a kit body 1, a drug mixing chamber 101 on the upper left side of the kit body 1, a drug mixing box 2 fixedly connected inside the drug mixing chamber 101, a detection display chamber 102 on the upper right side of the kit body 1, a rotating stirring tube 3 rotatably connected inside the drug mixing box 2, a sealing plug 4 fixedly connected to the right end of the rotating stirring tube 3, a connecting hole 104 through a partition between the drug mixing chamber 101 and the detection display chamber 102, a detection reagent outlet 204 on the right wall of the drug mixing box 2, and a blood sampling swab 5 located inside the rotating stirring tube 3. The blood sampling swab 5 is stored in the rotating stirring tube 3, effectively ensuring the cleanliness of the blood sampling swab 5. The blood sampling swab 5 and the kit body 1 are integrated into one unit for convenient use and handling.

[0030] In this embodiment, the reagent kit body 1 has a stirring tube inlet 103 at its left end, which is tightly connected to the rotating stirring tube 3. The reagent mixing box 2 has a sample inlet 201 at its top, and an inlet cap 202 is tightly fastened to the upper end of the sample inlet 201. The reagent mixing box 2 contains buffer solution. The left wall of the reagent mixing box 2 has a mixing box inlet 203, which is tightly connected to the rotating stirring tube 3. The mixing box inlet 203 and the stirring tube inlet 103 are coaxial. The right wall of the reagent mixing box 2 has a detection reagent outlet 204, which is a conical hole and coaxial with the inter-cavity connecting hole 104. The reagent mixing box 2 stores buffer solution. Under normal conditions, the reagent mixing box 2 is in a closed state. The mixing box inlet 203 and the detection reagent outlet 204 are tightly connected to the rotating stirring tube 3 to prevent the buffer solution inside the reagent mixing box 2 from leaking.

[0031] In this embodiment, four stirring blades 301 are fixedly connected to the outer wall of the rotating stirring tube 3, and a stirring handle 302 is fixedly connected to the left end of the rotating stirring tube 3. A connecting screw hole 303 is opened horizontally through the center of the stirring handle 302. After the blood sampling swab 5 containing the blood sample is placed into the medicine mixing box 2, the stirring blades 301 are rotated by rotating the rotating stirring tube 3, which stirs the buffer solution and the swab inside the medicine mixing box 2, helping the blood sample in the swab to quickly integrate into the buffer solution, thereby improving the fusion efficiency of the blood sample and the buffer solution.

[0032] In this embodiment, the right end of the sealing plug 4 is provided with a sealing cone surface 401, which is in close contact with the detection agent outlet 204. The right end of the sealing cone surface 401 is fixedly connected with a sealing insert 402, which is tightly inserted into the cavity connecting hole 104. Under normal conditions, the sealing plug 4, under the pushing action of the rotating stirring tube 3, makes the sealing cone surface 401 in close contact with the detection agent outlet 204, and the sealing insert 402 in close contact with the cavity connecting hole 104, thereby sealing the drug mixing box 2.

[0033] In Example 2, based on Example 1, a cotton swab spiral plug 501 is fixedly connected to the left end of the blood sampling swab 5. The cotton swab spiral plug 501 is spirally connected to the docking screw hole 303. A cotton swab handle 502 is fixedly connected to the left end of the cotton swab spiral plug 501. The spiral connection between the cotton swab spiral plug 501 and the docking screw hole 303 further improves the storage sealing of the blood sampling swab 5 and prevents the blood sampling swab 5 from being contaminated.

[0034] The working principle of this embodiment is as follows: First, rotate the handle 502 of the cotton swab to pull the blood sampling cotton swab 5 out of the rotating stirring tube 3. Collect a blood sample through the blood sampling cotton swab 5. Then, open the inlet cap 202, insert the cotton head of the blood sampling cotton swab 5 with the blood sample into the sample inlet 201 and break it off. Then, close the inlet cap 202 again and rotate the stirring tube handle 302 to make the rotating stirring tube 3 rotate inside the reagent mixing box 2. The stirring tube blades 301 stir the buffer solution and blood sample inside the reagent mixing box 2, helping the blood sample to mix in the buffer solution. After mixing, pull the stirring tube handle 302 to the left to move the sealing plug 4 to the left, so that the inside of the reagent mixing box 2 is connected to the detection reagent inside the detection display cavity 102. This allows the buffer solution inside the reagent mixing box 2 to enter the detection reagent for fusion, and the sample can then be detected by the detection reagent.

[0035] The following points should be noted in this article:

[0036] 1. The accompanying drawings of the embodiments disclosed herein only relate to the structures involved in the embodiments disclosed herein; other structures can be referred to in general design.

[0037] 2. Where there is no conflict, the embodiments of this disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.

[0038] The above are merely specific embodiments of this disclosure, but the scope of protection of this disclosure is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this disclosure should be included within the scope of protection of this disclosure. Therefore, the scope of protection of this disclosure should be determined by the scope of the claims.

Claims

1. An immunoassay kit for drug-induced liver injury markers in blood, comprising: The reagent kit box (1) is characterized in that a drug mixing chamber (101) is provided on the upper left side of the reagent kit box (1), a drug mixing box (2) is fixedly connected inside the drug mixing chamber (101), a detection display chamber (102) is provided on the upper right side of the reagent kit box (1), a rotating stirring tube (3) is rotatably connected inside the drug mixing box (2), a sealing plug (4) is fixedly connected to the right end of the rotating stirring tube (3), an inter-cavity connecting hole (104) is provided through the partition plate between the drug mixing chamber (101) and the detection display chamber (102), a detection reagent outlet (204) is provided on the right wall of the drug mixing box (2), and a blood sampling swab (5) is located inside the rotating stirring tube (3).

2. The immunoassay kit for drug-induced liver injury markers in blood according to claim 1, characterized in that, The left end of the reagent kit box (1) is provided with a stirring tube insertion port (103), which is tightly connected to the rotating stirring tube (3).

3. The immunoassay kit for drug-induced liver injury markers in blood according to claim 2, characterized in that, The sample inlet (201) is provided on the top of the drug mixing box (2), and the inlet cap (202) is tightly fastened to the upper end of the sample inlet (201). The drug mixing box (2) contains a buffer solution, and the left wall of the drug mixing box (2) has a mixing box insertion port (203).

4. The immunoassay kit for drug-induced liver injury markers in blood according to claim 3, characterized in that, The mixing box inlet (203) is tightly connected to the rotating stirring tube (3), and the mixing box inlet (203) and the stirring tube inlet (103) are coaxial.

5. The immunoassay kit for drug-induced liver injury markers in blood according to claim 4, characterized in that, The detection reagent outlet (204) is a conical hole, and the detection reagent outlet (204) is coaxial with the intercavity connecting hole (104).

6. The immunoassay kit for drug-induced liver injury markers in blood according to claim 5, characterized in that, Four stirring blades (301) are fixedly connected to the outer wall of the rotating stirring tube (3), and a stirring handle (302) is fixedly connected to the left end of the rotating stirring tube (3). A connecting screw hole (303) is opened horizontally through the center of the stirring handle (302).

7. The immunoassay kit for drug-induced liver injury markers in blood according to claim 6, characterized in that, The sealing plug (4) has a sealing cone surface (401) at the right end. The sealing cone surface (401) is in close contact with the detection agent outlet (204). A sealing insert (402) is fixedly connected to the right end of the sealing cone surface (401). The sealing insert (402) is tightly inserted into the cavity connecting hole (104).

8. The immunoassay kit for drug-induced liver injury markers in blood according to claim 7, characterized in that, The left end of the blood sampling swab (5) is fixedly connected to a swab spiral plug (501), the swab spiral plug (501) is spirally connected to the docking screw hole (303), and the left end of the swab spiral plug (501) is fixedly connected to a swab handle (502).

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