Immunohistochemical hydrophobic edge sealing device

Abstract

The immunohistochemical hydrophobic edge sealing device comprises a base and an upper pressing seat which is matched with the base in an up-and-down movement manner, the base comprises a bottom plate and a guide seat arranged at one end of the bottom plate, a working area is formed at the other end of the bottom plate, a guide hole is formed in the guide seat, and a spring is arranged in the guide hole; the upper pressing seat comprises a cross beam, a guide rod arranged at one end of the cross beam and a pressing head arranged at the other end of the cross beam, the guide rod is inserted into the guide hole and penetrates through the spring, the pressing head is arranged above the working area of the bottom plate and comprises a conical barrel, the conical barrel is composed of an outer wall and an inner wall, a liquid storage cavity is formed between the outer wall and the inner wall, and the liquid storage cavity is communicated with the liquid storage cavity. A seepage ring for blocking the liquid storage cavity is arranged at the large-opening end of the conical barrel, and the small-opening end of the conical barrel is in threaded connection with the cross beam. According to the utility model, the efficiency and quality of circle drawing and edge sealing can be improved.

Description

Technical Field

[0001] This utility model relates to the field of pathology laboratory tools technology, and in particular to an immunohistochemical hydrophobic sealing device. Background Technology

[0002] Immunohistochemistry (IHSC) is a technique that uses labeled, specific antibodies to determine the distribution of antigens in tissues and to detect cells in situ. It is widely used in clinical pathological diagnosis and research. During IHSC experiments, sealing the slide with an immunohistochemical pen is a crucial step to prevent antibody diffusion and spillage, reduce antibody and reagent usage, and significantly lower costs. However, existing immunohistochemical pens are time-consuming and labor-intensive when sealing the experimental tissue with a perimeter greater than 2 mm, and the quality of the perimeter is inconsistent, sometimes severely affecting the experimental process. Summary of the Invention

[0003] The technical problem to be solved by this utility model is to provide an immunohistochemical hydrophobic sealing device to improve the efficiency and quality of edge sealing.

[0004] To solve the above-mentioned technical problems, the technical solution of this utility model is: an immunohistochemical hydrophobic sealing device, including a base and an upper pressure seat that moves up and down with the base; the base includes a bottom plate and a guide seat at one end of the bottom plate, the other end of the bottom plate forms a working area, the guide seat is provided with a guide hole, and a spring is provided in the guide hole; the upper pressure seat includes a crossbeam, a guide rod at one end of the crossbeam and a pressure head at the other end of the crossbeam, the guide rod is inserted into the guide hole and passes through the spring, the pressure head is located above the working area of ​​the bottom plate, the pressure head includes a conical cylinder, the conical cylinder is composed of an outer wall and an inner wall, a liquid storage cavity is formed between the outer wall and the inner wall, a seepage ring is provided at the larger opening end of the conical cylinder to seal the liquid storage cavity, and the smaller opening end of the conical cylinder is threadedly connected to the crossbeam. The principle of this invention is as follows: When a glass slide is placed in the working area of ​​the base plate, the upper pressure seat is pressed down. The upper pressure seat moves downward against the elastic force of the spring and brings the pressure head closer to the glass slide. After the seepage ring at the lower end of the pressure head contacts the glass slide, the sealing liquid in the storage cavity seeps out into the seepage ring and remains on the glass slide to form a regular circular sealing edge. Using the base plate as a reference surface, the glass slide can be placed flat. With the cooperation of the circular seepage ring, a high-quality sealing edge can be quickly formed on the glass slide anytime and anywhere.

[0005] As an improvement, the crossbeam is a hollow structure, and the annular opening at the upper end of the liquid storage chamber is connected to the internal space of the crossbeam.

[0006] As an improvement, the upper end of the conical cylinder is provided with a threaded sleeve, and the end of the crossbeam is provided with a threaded post, the threaded sleeve and the threaded post cooperating.

[0007] As an improvement, the liquid storage chamber is equipped with absorbent cotton.

[0008] As an improvement, the working area of ​​the base plate is provided with a silicone pad.

[0009] As an improvement, the inner wall of the guide hole is provided with a first locking platform to lock the lower end of the spring, and the guide rod is provided with a second locking platform to lock the upper end of the spring.

[0010] As an improvement, the guide seat is provided with a clearance groove on the side facing the working area for the crossbeam to enter.

[0011] The beneficial effects of this utility model compared with the prior art are:

[0012] Using the base plate as a reference surface, the glass slide can be laid flat, and with the annular seepage ring, high-quality edge sealing can be quickly formed on the glass slide anytime, anywhere. Attached Figure Description

[0013] Figure 1 This is a cross-sectional view of the present invention. Detailed Implementation

[0014] The present invention will be further described below with reference to the accompanying drawings.

[0015] like Figure 1As shown, an immunohistochemistry hydrophobic sealing device includes a base and an upper pressure seat that moves up and down in coordination with the base. The base is used to place a glass slide, and the upper pressure seat is used to form an annular sealing edge. The base includes a rectangular base plate 1 and a guide seat 2 located at one end of the base plate 1; the other end of the base plate 1 forms a working area, and the working area of ​​the base plate 1 is provided with a silicone pad 11. During operation, the glass slide is held by hand and placed on the silicone pad 11, which can play a role in preventing slipping and collision, and better protecting the glass slide; the guide seat 2 is provided with a guide hole, and a spring 3 is provided in the guide hole. The inner wall of the guide hole is provided with a first locking platform that locks the lower end of the spring 3. The side of the guide seat 2 facing the working area is provided with a clearance groove for the crossbeam 5 to enter, and the crossbeam 5 moves up and down stably when passing through the clearance groove. The upper pressure seat includes a crossbeam 5, a guide rod 4 at one end of the crossbeam 5, and a pressure head 8 at the other end of the crossbeam 5. The guide rod 4 is inserted into a guide hole and passes through a spring 3. The guide rod 4 is provided with a second locking platform at the upper end of the locking spring 3. When the crossbeam 5 moves downward, it drives the guide rod 4, which compresses the spring 3. After the external force on the crossbeam 5 is removed, the crossbeam 5 moves upward and resets under the action of the spring 3. The crossbeam 5 is basically parallel to the base plate 1. The crossbeam 5 has a hollow structure, and its interior forms a storage space. The pressure head 8 is equivalent to a pen tip. The pressure head 8 is located above the working area of ​​the base plate 1. In the non-working state, the pressure head 8 is away from the base plate 1. In the working state, the crossbeam 5 is pressed so that the lower end of the pressure head 8 contacts the base plate 1. The pressure head 8 includes a conical cylinder, which is composed of an outer wall and an inner wall. The outer wall is parallel to the base plate 1. A liquid storage cavity is formed between the inner walls, and absorbent cotton 9 is provided in the liquid storage cavity to lock in the sealing liquid. The larger opening at the lower end of the conical cylinder is provided with a seepage ring 10 to seal the liquid storage cavity. The seepage ring 10 includes a skeleton with small holes and cotton cloth covering the skeleton. The sealing liquid can wet the seepage ring 10. When the seepage ring 10 is pressed on the glass slide, the sealing liquid of the seepage ring 10 will be imprinted on the glass slide. The middle of the conical cylinder is hollow, so it will not squeeze the glass slide. The smaller opening at the upper end of the conical cylinder is threadedly connected to the crossbeam 5. The upper end of the conical cylinder is provided with a threaded sleeve 7, and the end of the crossbeam 5 is provided with a threaded post 6. The threaded sleeve 7 and the threaded post 6 cooperate. After the pressure head 8 is connected to the crossbeam 5, the liquid storage cavity is connected to the internal space of the crossbeam 5, which increases the storage volume of the sealing liquid and facilitates the replacement of the pressure head 8.

[0016] The principle of this invention is as follows: When a glass slide is placed in the working area of ​​the base plate 1, the upper pressure seat is pressed down, and the upper pressure seat moves downward against the elastic force of the spring 3, bringing the pressure head 8 close to the glass slide. After the seepage ring 10 at the lower end of the pressure head 8 contacts the glass slide, the sealing liquid in the storage cavity seeps out into the seepage ring 10 and remains on the glass slide to form a regular circular sealing edge. Using the base plate 1 as a reference surface, the glass slide can be placed flat. With the help of the circular seepage ring 10, a high-quality sealing edge can be quickly formed on the glass slide anytime and anywhere.

Claims

1. An immunohistochemical hydrophobic sealing device, characterized in that: The device includes a base and an upper pressure seat that moves vertically in coordination with the base. The base includes a bottom plate and a guide seat at one end of the bottom plate. The other end of the bottom plate forms a working area. The guide seat has a guide hole, and a spring is installed in the guide hole. The upper pressure seat includes a crossbeam, a guide rod at one end of the crossbeam, and a pressure head at the other end of the crossbeam. The guide rod is inserted into the guide hole and passes through the spring. The pressure head is located above the working area of ​​the bottom plate. The pressure head includes a conical cylinder, which is composed of an outer wall and an inner wall. A liquid storage cavity is formed between the outer wall and the inner wall. A seepage ring is provided at the larger opening end of the conical cylinder to seal the liquid storage cavity. The smaller opening end of the conical cylinder is threadedly connected to the crossbeam.

2. The immunohistochemical hydrophobic sealing device according to claim 1, characterized in that: The crossbeam is a hollow structure, and the annular opening at the upper end of the liquid storage cavity is connected to the internal space of the crossbeam.

3. The immunohistochemical hydrophobic sealing device according to claim 1, characterized in that: The upper end of the conical cylinder is provided with a threaded sleeve, and the end of the crossbeam is provided with a threaded post, the threaded sleeve and the threaded post cooperating.

4. The immunohistochemical hydrophobic sealing device according to claim 1, characterized in that: The liquid storage chamber is equipped with absorbent cotton.

5. The immunohistochemical hydrophobic sealing device according to claim 1, characterized in that: The working area of ​​the base plate is equipped with a silicone pad.

6. The immunohistochemical hydrophobic sealing device according to claim 1, characterized in that: The inner wall of the guide hole is provided with a first locking platform to lock the lower end of the spring, and the guide rod is provided with a second locking platform to lock the upper end of the spring.

7. The immunohistochemical hydrophobic sealing device according to claim 1, characterized in that: The guide seat has a clearance groove on the side facing the working area for the crossbeam to enter.

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