Membrane protein separation and purification device

Abstract

The utility model discloses a membrane protein separation and purification device and relates to the technical field of protein separation and purification. The device comprises a shell. According to the utility model, the fixing assembly is pressed downwards, so that the height of the fixing assembly is reduced, then the separation assembly is put into the shell, then the fixing assembly is loosened, so that the separation assembly is pushed to be connected to the bottom end of the feeding assembly in a sleeving manner, and then a crushed membrane protein mixed solution is poured into the feeding assembly, so that the crushed membrane protein mixed solution can enter the separation assembly; the method comprises the following steps: separating a membrane protein mixed solution by virtue of a separation assembly, purifying target protein from other impurities by virtue of the separation assembly, and when the interior of the separation assembly needs to be cleaned, pulling a clamping assembly upwards so as to release clamping and fixing of the separation assembly, and then pulling the separation assembly so as to separate the separation assembly, therefore, the separation assembly can be cleaned conveniently, and follow-up use is facilitated.

Description

Technical Field

[0001] This invention belongs to the field of protein separation and purification technology, and specifically relates to a membrane protein separation and purification device. Background Technology

[0002] Membrane proteins are proteins that are embedded in or bound to biological membranes. They are the main executors of biological membrane functions, playing a crucial role in many processes such as cellular transport, energy conversion, and signal transduction.

[0003] Bioengineering and biotechnology generally involve the separation and purification of membrane proteins for the construction of biosensors, enabling them to detect specific biomolecules or physicochemical signals. Existing membrane protein separation and purification devices are typically a single unit with multiple layers of filter membranes inside to separate membrane proteins and purify the target protein from other impurities. However, after the membrane proteins are separated and purified, the mixture of membrane proteins adhering to the inner wall is difficult to clean, and the filter membranes are not easy to disassemble, which can easily affect subsequent use.

[0004] There are currently no effective solutions to the problems in the relevant technologies. Utility Model Content

[0005] In view of the problems in the related technologies, this utility model proposes a membrane protein separation and purification device to overcome the above-mentioned technical problems existing in the existing related technologies.

[0006] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0007] This utility model relates to a membrane protein separation and purification device, comprising a shell:

[0008] The housing is respectively provided with a feeding component, a separating component, a fixing component, and a snap-fit ​​component;

[0009] The feeding component is internally connected to the separation component, so that the feeding component introduces the broken membrane proteins into the separation component for separation.

[0010] The top end of the fixing component is attached to the bottom end of the separating component so that the fixing component supports and fixes the separating component.

[0011] The snap-fit ​​component has one side that fits against one side of the separation component, so that the snap-fit ​​component snaps and fixes the separation component in place.

[0012] Furthermore, the feeding assembly includes a feeding hopper, the outer surface of which is fixedly connected to the inner wall of the housing, and a guide tube is fixedly connected to the bottom end of the feeding hopper.

[0013] Furthermore, the separation assembly includes a connecting tube, the inside of which is sleeved with the outer surface of the guide tube, a separation box is fixedly connected to the bottom end of the connecting tube, multiple sets of filter membranes are slidably arranged inside the separation box, and a purification tube is threadedly installed at the bottom end of the separation box.

[0014] Furthermore, the fixing component includes a mounting frame, one side of which is fixedly connected to the inner wall of the housing, a limiting rod is fixedly connected inside the mounting frame, and a sliding block is fixedly connected to the outer surface of the limiting rod, with the top end of the sliding block abutting against the bottom end of the separation box.

[0015] Furthermore, the fixing assembly also includes a spring and a pressure block, one end of the spring being fixedly connected to the interior of the mounting frame, the other end of the spring being fixedly connected to the bottom end of the sliding block, and the top end of the pressure block being fixedly connected to the bottom end of the sliding block.

[0016] Furthermore, the snap-fit ​​assembly includes a guide rod, both ends of which are fixedly connected to the inner wall of the separation box. A guide block is slidably connected to the outer surface of the guide rod, and a tension spring is fixedly connected to the bottom end of the guide block. The bottom end of the tension spring is fixedly connected to the inner wall of the separation box.

[0017] The guide block is equipped with a pull rod that rotates inside, and a handle is fixedly connected to the top of the pull rod.

[0018] Furthermore, the snap-fit ​​assembly also includes a connecting plate, one side of which is fixedly connected to one side of the guide block, and a wedge block is fixedly connected to one side of the connecting plate, one side of which is in contact with one side of the filter membrane.

[0019] This utility model has the following beneficial effects:

[0020] 1. This utility model involves pressing down on the fixing component to lower its height, then placing the separation component inside the housing. The fixing component is then released, allowing it to push the separation component onto the bottom of the feeding component. The broken membrane protein mixture is then poured into the feeding component, allowing it to enter the separation component. This enables the separation component to separate the membrane protein mixture and purify the target protein from other impurities. When cleaning the inside of the separation component is required, the locking component is pulled upwards to release its locking mechanism, allowing the separation component to be pulled apart for easy cleaning and subsequent use.

[0021] 2. This utility model moves the sliding block fixed at the top by pressing down the pressure block, so that it can compress the spring along the direction of the limiting rod. At the same time, it can move the separation box attached to the top of the spring downward, so that the connecting tube and the guide tube can be separated, so that the separation box can be taken out from the inside of the shell for cleaning or to remove the purified membrane protein.

[0022] 3. This utility model moves the bottom-fixed pull rod by pulling the handle upward, which in turn moves the guide block rotated on the outer surface upward. This causes the guide block to stretch the bottom-fixed tension spring along the direction of the guide rod, and at the same time moves the connecting plate fixed on one side, which in turn moves the wedge block fixed on one side upward. This releases the wedge block from limiting the filter membrane, making it easier to pull the filter membrane out of the separation box for cleaning.

[0023] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0024] To more clearly illustrate the technical solutions of the utility model embodiments, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0026] Figure 2 This is a top-view structural schematic diagram of the present invention;

[0027] Figure 3 This is a schematic diagram of the cross-sectional structure of the present invention from a left-side view.

[0028] Figure 4 For the present utility model Figure 3 Enlarged schematic diagram of the local structure at point A;

[0029] Figure 5 This is a schematic diagram of the internal structure of the fixing component of this utility model;

[0030] Figure 6 This is a schematic diagram of the internal structure of the separation box of this utility model.

[0031] The attached diagram lists the components represented by each number as follows:

[0032] 1. Shell; 2. Feeding assembly; 201. Feeding hopper; 202. Guide tube; 3. Separation assembly; 301. Connecting tube; 302. Separation box; 303. Filter membrane; 304. Purification tube; 4. Fixing assembly; 401. Mounting frame; 402. Limiting rod; 403. Sliding block; 404. Spring; 405. Pressure block; 5. Snap-fit ​​assembly; 501. Guide rod; 502. Guide block; 503. Tension spring; 504. Pull rod; 505. Handle; 506. Connecting plate; 507. Wedge block. Detailed Implementation

[0033] The technical solutions of the utility model embodiments will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the utility model, and not all embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the utility model.

[0034] In the description of this utility model, it should be understood that the terms "opening", "upper", "lower", "top", "middle", "inner", etc., which indicate orientation or positional relationship, are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the utility model.

[0035] Please see Figures 1-6 As shown, this utility model is a membrane protein separation and purification device, including a shell 1:

[0036] The housing 1 is respectively provided with a feeding component 2, a separating component 3, a fixing component 4, and a snap-fit ​​component 5;

[0037] The feeding component 2 is internally connected to the separation component 3, so that the feeding component 2 introduces the broken membrane protein into the separation component 3 for separation.

[0038] The top end of the fixing component 4 is attached to the bottom end of the separating component 3 so that the fixing component 4 supports and fixes the separating component 3.

[0039] The snap-fit ​​component 5 has one side that fits against one side of the separation component 3 so that the snap-fit ​​component 5 snaps and fixes the separation component 3.

[0040] In use, press down on the fixing component 4 to lower its height, then place the separation component 3 inside the housing 1 so that the bottom of the separation component 3 fits against the top of the fixing component 4, and the top of the separation component 3 is coaxial with the feeding component 2. Then release the fixing component 4, and the separation component 3 can be pushed onto the bottom of the feeding component 2 by its own reset ability, so that the interior of the separation component 3 is connected to the interior of the feeding component 2. Then pour the broken membrane protein mixture into the interior of the feeding component 2 so that it can enter the interior of the separation component 3, so that the separation component 3 can separate the membrane protein mixture and purify the target protein from other impurities. When it is necessary to clean the interior of the separation component 3, pull up the locking component 5 to release it from the locking of the separation component 3, then pull the separation component 3 to disassemble it and then clean it.

[0041] This invention allows the fixing component 4 to be lowered by pressing it down, then the separation component 3 is placed inside the housing 1. The fixing component 4 is then released, allowing the separation component 3 to be fitted onto the bottom of the feeding component 2. The broken membrane protein mixture is then poured into the feeding component 2, allowing it to enter the separation component 3. This enables the separation component 3 to separate the membrane protein mixture and purify the target protein from other impurities. When cleaning the interior of the separation component 3 is required, the locking component 5 is pulled upwards to release the locking mechanism, and then the separation component 3 is pulled to disassemble it, facilitating cleaning and subsequent use.

[0042] In one embodiment, the feeding assembly 2 includes a feeding hopper 201, the outer surface of which is fixedly connected to the inner wall of the housing 1, and a guide tube 202 is fixedly connected to the bottom end of the feeding hopper 201.

[0043] The feeding hopper 201 facilitates the feeding of the membrane protein mixture, and the guide tube 202 facilitates the guidance of the membrane protein mixture into the interior of the separation component 3, thereby ensuring its stability during the separation and purification process.

[0044] In one embodiment, the separation component 3 includes a connecting tube 301, the interior of which is sleeved with the outer surface of the guide tube 202, a separation box 302 is fixedly connected to the bottom end of the connecting tube 301, a plurality of filter membranes 303 are slidably arranged inside the separation box 302, and a purification tube 304 is threadedly installed at the bottom end of the separation box 302.

[0045] When the membrane protein mixture enters the interior of the connecting tube 301 through the guide tube 202, it can pass through multiple sets of filter membranes 303 set inside the separation chamber 302 in one go for multi-stage separation, so that the separated and purified target protein can enter the interior of the purification tube 304. Since the outer surface of the purification tube 304 is threadedly connected to the bottom end of the separation chamber 302, it is easy to disassemble it, so as to facilitate the subsequent processing of the purified protein inside the purification tube 304.

[0046] In one embodiment, the fixing component 4 includes a mounting frame 401, one side of which is fixedly connected to the inner wall of the housing 1. A limiting rod 402 is fixedly connected inside the mounting frame 401, and a sliding block 403 is fixedly connected to the outer surface of the limiting rod 402. The top end of the sliding block 403 is in contact with the bottom end of the separation box 302.

[0047] The fixing component 4 also includes a spring 404 and a pressure block 405. One end of the spring 404 is fixedly connected to the inside of the mounting frame 401, and the other end of the spring 404 is fixedly connected to the bottom end of the sliding block 403. The top end of the pressure block 405 is fixedly connected to the bottom end of the sliding block 403.

[0048] By pressing down on the pressure block 405, the sliding block 403 fixed at the top is moved, so that it can compress the spring 404 along the direction of the limiting rod 402. At the same time, it can move the separation box 302, which is attached to the top, downward, so that the connecting tube 301 is disengaged from the guide tube 202, so that the separation box 302 can be taken out from the inside of the shell 1 for cleaning or removal of purified membrane proteins.

[0049] In one embodiment, the snap-fit ​​assembly 5 includes a guide rod 501, both ends of which are fixedly connected to the inner wall of the separation box 302. A guide block 502 is slidably connected to the outer surface of the guide rod 501. A tension spring 503 is fixedly connected to the bottom end of the guide block 502. The bottom end of the tension spring 503 is fixedly connected to the inner wall of the separation box 302.

[0050] The guide block 502 is rotatably provided with a pull rod 504, and a handle 505 is fixedly connected to the top end of the pull rod 504;

[0051] The snap-fit ​​assembly 5 also includes a connecting plate 506, one side of which is fixedly connected to one side of the guide block 502, and a wedge block 507 is fixedly connected to one side of the connecting plate 506, one side of which is in contact with one side of the filter membrane 303.

[0052] By pulling the handle 505 upwards, the pull rod 504 fixed at the bottom end is moved, which in turn moves the guide block 502 rotatably mounted on the outer surface upwards. This causes the guide block 502 to stretch the tension spring 503 fixed at the bottom end along the direction of the guide rod 501. At the same time, it can move the connecting plate 506 fixed on one side, which in turn moves the wedge block 507 fixed on one side upwards. This releases the wedge block 507 from the filter membrane 303, making it easier to pull the filter membrane 303 out of the separation box 302 for cleaning.

[0053] Since one side of the filter membrane 303 is wedge-shaped, when the filter membrane 303 is inserted into the separation box 302, the wedge-shaped surface of the filter membrane 303 can fit against the wedge-shaped surface of the wedge block 507, and push the wedge block 507 to move upward, thereby facilitating the installation of the filter membrane 303.

[0054] Through the above technical solution, 1. By pressing down the fixing component 4 to lower its height, the separation component 3 is placed inside the housing 1. Then, the fixing component 4 is released to push the separation component 3 to fit onto the bottom of the feeding component 2. Then, the broken membrane protein mixture is poured into the feeding component 2 so that it can enter the interior of the separation component 3, so that the separation component 3 can separate the membrane protein mixture and purify the target protein from other impurities. When it is necessary to clean the interior of the separation component 3, the snap-fit ​​component 5 is pulled upward to release the snap-fit ​​fixing of the separation component 3. Then, the separation component 3 is pulled to disassemble it, which facilitates cleaning of the separation component 3 and subsequent use.

[0055] 2. By pressing down on the pressure block 405, the sliding block 403 fixed at the top is moved so that it can compress the spring 404 along the direction of the limiting rod 402. At the same time, it can move the separation box 302, which is attached to the top, downward so that the connecting tube 301 is disengaged from the guide tube 202, so that the separation box 302 can be taken out from the inside of the shell 1 for cleaning or removal of purified membrane proteins.

[0056] 3. By pulling the handle 505 upward, the pull rod 504 fixed at the bottom end is moved, which in turn moves the guide block 502 rotatably mounted on the outer surface upward. This causes the guide block 502 to stretch the tension spring 503 fixed at the bottom end along the direction of the guide rod 501. At the same time, it can move the connecting plate 506 fixed on one side, which in turn moves the wedge block 507 fixed on one side upward. This releases the wedge block 507 from the filter membrane 303, making it easier to pull the filter membrane 303 out of the separation box 302 for cleaning.

[0057] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0058] The preferred embodiments of the utility model disclosed above are merely illustrative of the utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the utility model, thereby enabling those skilled in the art to better understand and utilize it. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A membrane protein separation and purification device, comprising a shell (1), characterized in that: The shell (1) is provided with a feeding assembly (2), a separation assembly (3), a fixing assembly (4) and a clamping assembly (5) respectively; The inside of the feeding assembly (2) is communicated with the inside of the separation assembly (3), so that the feeding assembly (2) introduces the broken membrane protein into the inside of the separation assembly (3) for separation; The top end of the fixing assembly (4) is matched with the bottom end of the separation assembly (3), so that the fixing assembly (4) supports and fixes the separation assembly (3); One side of the clamping assembly (5) is matched with one side of the separation assembly (3), so that the clamping assembly (5) clamps and fixes the separation assembly (3).

2. The apparatus for separating and purifying a membrane protein according to claim 1, wherein The feeding assembly (2) comprises a feeding hopper (201), the outer surface of the feeding hopper (201) is fixedly connected with the inner wall of the shell (1), and the bottom end of the feeding hopper (201) is fixedly connected with a guide pipe (202).

3. The apparatus for separating and purifying a membrane protein according to claim 2, wherein The separation assembly (3) comprises a connecting pipe (301), the inside of the connecting pipe (301) is sleeved with the outer surface of the guide pipe (202), the bottom end of the connecting pipe (301) is fixedly connected with a separation box (302), the inside of the separation box (302) is slidably provided with a plurality of filter membranes (303), and the bottom end of the separation box (302) is threadedly installed with a purification pipe (304).

4. The apparatus for separating and purifying membrane proteins according to claim 3, wherein The fixing assembly (4) comprises a mounting frame (401), one side of the mounting frame (401) is fixedly connected with the inner wall of the shell (1), the inside of the mounting frame (401) is fixedly connected with a limiting rod (402), the outer surface of the limiting rod (402) is fixedly connected with a sliding block (403), and the top end of the sliding block (403) is matched with the bottom end of the separation box (302).

5. The apparatus for separating and purifying a membrane protein according to claim 4, wherein The fixing assembly (4) further comprises a spring (404) and a pressing block (405), one end of the spring (404) is fixedly connected with the inside of the mounting frame (401), the other end of the spring (404) is fixedly connected with the bottom end of the sliding block (403), and the top end of the pressing block (405) is fixedly connected with the bottom end of the sliding block (403).

6. The apparatus for separating and purifying membrane proteins according to claim 3, wherein The clamping assembly (5) comprises a guide rod (501), both ends of the guide rod (501) are fixedly connected with the inner wall of the separation box (302), the outer surface of the guide rod (501) is slidably connected with a guide block (502), the bottom end of the guide block (502) is fixedly connected with a tension spring (503), and the bottom end of the tension spring (503) is fixedly connected with the inner wall of the separation box (302); The inside of the guide block (502) is rotatably provided with a pull rod (504), and the top end of the pull rod (504) is fixedly connected with a handle (505).

7. The device for separating and purifying membrane proteins according to claim 6, wherein The clamping assembly (5) further comprises a connecting plate (506), one side of the connecting plate (506) is fixedly connected with one side of the guide block (502), one side of the connecting plate (506) is fixedly connected with a wedge block (507), and one side of the wedge block (507) is matched with one side of the filter membrane (303).

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