High-precision trace automatic moving and taking device
By combining components such as the triaxial pipetting assembly and the oscillating lysis assembly, the problem of insufficient precision in existing devices is solved, achieving high-precision liquid transfer and cell lysis, improving the accuracy and repeatability of bacterial detection, and preventing contamination.
Patent Information
- Application Number
- CN202422977074.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-04
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2034-12-04
AI Technical Summary
Existing automated transfer devices cannot achieve sufficient accuracy, especially when handling small-volume samples, which can easily lead to deviations and errors in the bacterial detection process.
Employing a triaxial pipetting assembly, an oscillating lysis assembly, a pipette tip feeding assembly, and a heating culture assembly, the pipette is precisely positioned in three-dimensional space via a triaxial track and lead screw. Combined with a vibration spring and temperature control, this ensures accurate liquid transfer and thorough cell lysis.
It achieves high-precision liquid transfer and cell lysis, avoids human error, improves the accuracy and repeatability of detection, prevents cross-contamination, and ensures the reliability of experimental results.
Smart Images
Figure CN223852616U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of cell culture technology, specifically, it relates to a high-precision micro-volume automatic transfer device. Background Technology
[0002] The collection and detection of airborne bacteria are commonly used in air quality assessment, environmental sanitation, and disease prevention. In these fields, the bacterial content in the air directly affects the accuracy of air quality assessments. While traditional manual sampling and detection methods are effective, operational errors and the cumbersome sampling and transfer processes can easily lead to unstable and unreliable results. Existing devices that separate and extract airborne bacterial cells using a collection device, transfer them to a lysing device for lysis, and finally perform detection significantly improve the efficiency and accuracy of testing.
[0003] Micro-volume automated transfer devices automatically extract, transfer, and process minute amounts of samples, ensuring accuracy, repeatability, and efficiency in sampling and processing.
[0004] However, existing automated transfer devices cannot achieve sufficient accuracy, especially when processing small-volume samples, which can easily lead to deviations and errors in the bacterial detection process. Utility Model Content
[0005] In view of this, the present invention provides a high-precision micro-volume automatic transfer device, which can solve the problem that existing automatic transfer devices cannot achieve sufficient precision, especially when processing small volume samples, which is prone to deviation and leads to errors in the bacterial detection process.
[0006] This utility model is implemented as follows:
[0007] This invention provides a high-precision, micro-volume automated pipetting device, comprising a triaxial pipetting assembly, an oscillating lysis assembly, a pipette tip feeding assembly, a waste assembly, and a heating and culture assembly. The triaxial pipetting assembly is disposed on top of the oscillating lysis assembly, the pipette tip feeding assembly, the waste assembly, and the heating and culture assembly. The triaxial pipetting assembly includes a triaxial track and a pipette. The pipette is fixed on the triaxial track and moves under the drive of the track to extract the liquid after oscillating lysis and replace the pipette tip. The oscillating lysis assembly is fixed to one side of the bottom of the triaxial pipetting assembly and is used to oscillate the collected cells. The pipette tip feeding assembly and the waste assembly are disposed on the other side of the triaxial pipetting assembly and are used to provide new pipette tips and collect used tips to avoid contamination. The heating and culture assembly has multiple liquid inlets on its top for heating and culturing the cells.
[0008] On the basis of the above technical scheme, the high-precision micro automatic pick-and-place device can be further improved as follows:
[0009] The three-axis track comprises two slide rods, a first lead screw, a second lead screw and a third lead screw, the two slide rods are fixed in parallel on the two sides, the first lead screw is fixed in parallel with the slide rod on the top of one side of the slide rod, the second lead screw is slidably connected to the first lead screw, the third lead screw is slidably connected to the second lead screw, and the pipette is slidably connected to the third lead screw.
[0010] Further, the included angle between the first lead screw, the second lead screw and the third lead screw is 90°.
[0011] Further, the two sides of the second lead screw are slidably connected to the two slide rods, and the length of the second lead screw matches the distance between the two first lead screws.
[0012] Further, one end of the first lead screw, the second lead screw and the third lead screw is connected with a motor, the motor drives the first lead screw, the second lead screw and the third lead screw to rotate to drive the pipette to move in three-dimensional directions.
[0013] Further, a plurality of vibration springs are arranged at the middle joint of the oscillation and lysis assembly.
[0014] Further, a temperature monitoring and control device is arranged at the port of the oscillation and lysis assembly.
[0015] Further, a plurality of pipette positioning openings are arranged at the top of the pipette feeding assembly, and a pipette is inserted into the openings.
[0016] Further, an opening is arranged at the top of the waste material assembly for discarding used pipettes.
[0017] Further, the opening of the oscillation and lysis assembly is arranged on the same horizontal line with the liquid inlet of the heating and culturing assembly, and is arranged in parallel with the second lead screw; and the opening centers of the pipette feeding assembly and the waste material assembly are arranged on the same horizontal line, and are arranged in parallel with the second lead screw.
[0018] Compared with the prior art, the high-precision micro automatic pick-and-place device has the following advantages:
[0019] The three-axis pipetting assembly is the core part of the device, responsible for accurately moving the pipette in three directions, enabling precise control of liquid transfer. It consists of a three-axis track and a pipette fixed on it. The pipette can be precisely positioned and operated in three axes by the three-axis track. By precisely controlling the movement of the pipette, the accuracy of liquid transfer is ensured, avoiding errors caused by manual operation.
[0020] The three-axis track is composed of a slide bar and multiple lead screws. The slide bar is fixed on both sides, and the lead screws drive the pipette to move in three-dimensional space. The pipette is fixed on the three-axis track and can move in different directions to complete functions such as liquid extraction and replacing the tip. By setting multiple lead screws and connecting them, the pipette is driven to move freely in three-dimensional space, improving the accuracy and flexibility of pipetting operations. The motor drives the lead screw to rotate, thereby driving the three-dimensional movement of the pipette, achieving high-precision operation.
[0021] The oscillation lysis assembly is responsible for oscillating and lysing cells, which is commonly used to break the cell wall or cell membrane in cell samples to release the cell contents. This process is widely used in cell biology experiments.
[0022] By setting the vibration spring, the oscillation lysis assembly can generate vibration to effectively lyse cells through the vibration of the spring. The temperature monitoring and control device is located at the port position of the lysis assembly, which can help provide the necessary temperature conditions to optimize the cell lysis process. By setting multiple vibration springs, the lysis effect is more uniform, which can lyse cells more thoroughly. Ensuring that the lysis process is carried out at an appropriate temperature helps improve the efficiency of cell lysis.
[0023] The pipette tip is used to extract and transfer liquid samples during liquid collection. The high-precision tip design ensures accurate liquid transfer and avoids cross-contamination. The top of the tip supply assembly is provided with multiple openings to facilitate the insertion of different types of tips.
[0024] The waste assembly is used to store and dispose of used tips, preventing contamination and ensuring the cleanliness of the device's internal environment. The opening design at the top of the waste assembly facilitates the placement and collection of used tips, preventing the spread of bacteria or other contaminants.
[0025] The heating incubation assembly is responsible for providing suitable temperatures to support the heating incubation of cells. Multiple liquid inlets can help accurately control the addition of culture liquid while performing temperature control operations. The heating incubation assembly ensures that the temperature of cell culture or chemical reactions is maintained within an appropriate range through a heating control system.
[0026] The liquid inlet is on the same horizontal line as the opening position of the oscillation lysis assembly, which facilitates synchronous operation and ensures the accuracy of liquid addition and temperature uniformity during the heating incubation process. Attached Figure Description
[0027] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments of this utility model will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0028] Fig. 1 This is a schematic diagram of a high-precision, micro-volume automatic transfer device.
[0029] Fig. 2 This is a schematic diagram of the structure of a triaxial pipetting assembly;
[0030] The attached diagram lists the components represented by each number as follows:
[0031] 10. Triaxial pipetting assembly; 11. Triaxial track; 111. Slide rod; 112. First lead screw; 113. Second lead screw; 114. Third lead screw; 12. Pipette; 20. Oscillating lysis assembly; 30. Pipette tip feeding assembly; 40. Disposal assembly; 50. Heating and incubation assembly. Detailed Implementation
[0032] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings.
[0033] like Figs. 1-2 The diagram illustrates an embodiment of a high-precision micro-volume automatic transfer device provided by this utility model. This embodiment includes a triaxial pipetting assembly 10, an oscillating lysis assembly 20, a pipette tip feeding assembly 30, a waste assembly 40, and a heating and culture assembly 50. The triaxial pipetting assembly 10 is positioned on top of the oscillating lysis assembly 20, the pipette tip feeding assembly 30, the waste assembly 40, and the heating and culture assembly 50. The triaxial pipetting assembly 10 includes a triaxial track 11 and a pipette 12. The pipette 12 is fixed to the triaxial track 11 and moves under the drive of the triaxial track 11 to extract the liquid after oscillating lysis and replace the pipette tip. The oscillating lysis assembly 20 is fixed to one side of the bottom of the triaxial pipetting assembly 10 and is used to oscillate the collected cells. The pipette tip feeding assembly 30 and the waste assembly 40 are located on the other side of the triaxial pipetting assembly 10 and are used to provide new pipette tips and collect used pipette tips to avoid contamination. The heating and culture assembly 50 has multiple liquid inlets at its top for heating and culturing the cells.
[0034] In the technical scheme, the three-axis track 11 comprises two slide rods 111, a first screw rod 112, a second screw rod 113 and a third screw rod 114. The two slide rods 111 are fixed in parallel on two sides. The first screw rod 112 is fixed in parallel with the slide rod 111 on the top of one side of the slide rod 111. The second screw rod 113 is slidingly connected to the first screw rod 112. The third screw rod 114 is slidingly connected to the second screw rod 113. The pipette gun 12 is slidingly connected to the third screw rod 114.
[0035] Further, in the technical scheme, the included angles between the first screw rod 112, the second screw rod 113 and the third screw rod 114 are all 90°.
[0036] Further, in the technical scheme, the second screw rod 113 is slidingly connected to the two slide rods 111 on two sides. The length of the second screw rod 113 matches the distance between the two first screw rods 112.
[0037] Further, in the technical scheme, the first screw rod 112, the second screw rod 113 and the third screw rod 114 are all connected to a motor at one end. The motor drives the first screw rod 112, the second screw rod 113 and the third screw rod 114 to rotate, so as to drive the pipette gun 12 to move in three-dimensional directions.
[0038] Further, in the technical scheme, the middle joint of the oscillation and lysis assembly 20 is provided with a plurality of vibration springs.
[0039] Further, in the technical scheme, the port of the oscillation and lysis assembly 20 is provided with a temperature monitoring and control device.
[0040] Further, in the technical scheme, the top of the gun head feeding assembly 30 is provided with a plurality of gun head positioning openings. The openings are inserted with gun heads.
[0041] Further, in the technical scheme, the top of the waste assembly 40 is provided with an opening for discarding used gun heads.
[0042] Further, in the technical scheme, the opening of the oscillation and lysis assembly 20 is on the same horizontal line with the liquid inlet of the heating and culturing assembly 50, and is arranged in parallel with the second screw rod 113. The opening centers of the gun head feeding assembly 30 and the waste assembly 40 are on the same horizontal line, and are arranged in parallel with the second screw rod 113.
[0043] Specifically, the principle of the utility model is: when using, install the pipette correctly to the three-axis pipetting assembly, ensure its stability and free movement; set the pipette tip: according to the experimental needs, select the appropriate model of pipette tip and install it on the pipette. Configure the oscillation frequency and time of the oscillation lysis assembly, adjust the temperature setting of the heating culture assembly. According to the needs, start the oscillation lysis function. The system will automatically start the oscillation treatment of the cell sample, and the cell membrane or cell wall is lysed to release the cell contents. Move the pipette to the designated position through the three-axis pipetting assembly, and automatically move the liquid. The pipette will move accurately above the liquid level of the liquid container, and the pipette will suck the specified volume of liquid and transfer it to the target container. Ensure that air is not sucked in or liquid is left in the gun head during the pipetting process. When cell culture or chemical reaction is needed, start the heating culture function. The system will place the target sample in the heating culture area and control the heating equipment to provide stable temperature. The pipette automatically adds the lysed cell solution to the culture area. After the pipetting operation is completed, the used gun head is automatically discarded into the waste assembly, ensuring that cross contamination does not occur. Replace the new gun head to avoid the influence of the experimental results caused by the gun head pollution.
Claims
1. A high-precision micro-amount automatic pick-up device characterized by comprising: The application relates to a three-axis pipette assembly (10), an oscillation lysis assembly (20), a tip supply assembly (30), a waste assembly (40) and a heated incubation assembly (50), wherein the three-axis pipette assembly (10) is arranged on the top of the oscillation lysis assembly (20), the tip supply assembly (30) and the waste assembly (40) and the heated incubation assembly (50), the three-axis pipette assembly (10) comprises a three-axis track (11) and a pipette gun (12) fixed on the three-axis track (11) and used for moving under the drive of the three-axis track (11) to extract the liquid after oscillation lysis and replace the tip; the oscillation lysis assembly (20) is fixed on one side of the bottom of the three-axis pipette assembly (10) and used for oscillation lysis of collected cells; the tip supply assembly (30) and the waste assembly (40) are arranged on the other side of the three-axis pipette assembly (10) and used for supplying new pipette tips and collecting used pipette tips to avoid pollution; and the heated incubation assembly (50) is provided with a plurality of liquid inlets on the top and used for heated incubation of cells.
2. The high-precision micro-amount automatic pipetting device according to claim 1, wherein The three-axis track (11) comprises two slide rods (111), a first lead screw (112), a second lead screw (113) and a third lead screw (114), the two slide rods (111) are fixed in parallel on two sides, the first lead screw (112) is fixed on the top of one side of the slide rod (111) in parallel with the slide rod (111), the second lead screw (113) is slidably connected to the first lead screw (112), the third lead screw (114) is slidably connected to the second lead screw (113), and the pipette gun (12) is slidably connected to the third lead screw (114).
3. The high-precision micro-amount automatic pipetting device according to claim 2, wherein The included angles between the first lead screw (112), the second lead screw (113) and the third lead screw (114) are all 90 degrees.
4. The high-precision micro-amount automatic pipetting device according to claim 3, wherein The second lead screw (113) is slidably connected to the two slide rods (111) on two sides, and the length of the second lead screw (113) matches the distance between the two first lead screws (112).
5. The high-precision micro-amount automatic pipetting device according to claim 4, wherein One end of the first lead screw (112), the second lead screw (113) and the third lead screw (114) is connected with a motor, the motor drives the first lead screw (112), the second lead screw (113) and the third lead screw (114) to rotate to drive the pipette gun (12) to move in three-dimensional directions.
6. The high-precision micro-amount automatic pipetting device according to claim 5, wherein A plurality of vibration springs are arranged at the middle joint of the oscillation lysis assembly (20).
7. The high-precision micro-amount automatic pipetting device according to claim 6, wherein A temperature monitoring and control device is arranged at the port of the oscillation lysis assembly (20).
8. The high-precision micro-amount automatic pipetting device according to claim 7, wherein A plurality of tip positioning openings are arranged on the top of the tip supply assembly (30), and tips are inserted into the openings of the tip positioning openings.
9. The high-precision micro-amount automatic pipetting device according to claim 8, wherein An opening is arranged on the top of the waste assembly (40) and used for discarding used tips.
10. The high-precision micro-amount automatic pipetting device according to claim 9, wherein The opening position of the oscillation cracking assembly (20) is on the same horizontal line with the liquid inlet of the heating culture assembly (50) and is arranged in parallel with the second lead screw (113); the opening centers of the gun head feeding assembly (30) and the waste assembly (40) are on the same horizontal line and are arranged in parallel with the second lead screw (113).