Micro-fluidic chip for PCR (Polymerase Chain Reaction) amplification

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) amplified micro-fluidic chip which comprises a micro-fluidic chip body, a temperature control system is arranged above the micro-fluidic chip body, an amplification bin is arranged inside the micro-fluidic chip body, a detection interface is arranged above the micro-fluidic chip body, and the detection interface is connected with the amplification bin. A cleaning liquid feeding pipe is arranged on the side face of the detection connector, a feeding box is arranged on the side face of the temperature control system, and four sample injection ports are fixedly connected to the upper portion of the feeding box. By means of the structure, the problems that when traditional PCR amplification is carried out, enzyme participating in reaction is very sensitive to temperature, temperature fluctuation possibly leads to unstable activity of polymerase, the amplification speed and the product quality are affected, but the temperature in an amplification bin of an existing micro-fluidic chip is likely to be unstable, and the amplification efficiency and the product quality are affected can be solved. The problem that the enzyme may be unstable during PCR amplification is avoided, and the condition that the product is unstable due to unstable temperature is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic chip technical field especially relates to a PCR amplification's micro -fluidic chip. BACKGROUND

[0002] Polymerase chain reaction (PCR), it is a kind of DNA's rapid amplification technology, is the necessary means of real-time fluorescent quantitative PCR, gene sequencing and molecular diagnosis such as gene chip.PCR is by DNA in vitro 95 ℃ high temperature denaturation becomes single strand, primer and single strand are combined according to the principle of base complementary pairing at low temperature (about 60 ℃), again adjust temperature to the optimum reaction temperature (about 72 ℃) of DNA polymerase, and DNA polymerase is synthesized complementary chain along the direction of phosphate to pentose.

[0003] In prior art, when traditional PCR amplifies, the enzyme participating in the reaction is very sensitive to temperature, and the fluctuation of temperature can lead to unstable activity of polymerase, affect the speed of amplification and the quality of product, but the existing micro -fluidic chip can appear the situation of unstable temperature in amplification bin, directly lead to the problem of unstable enzyme during PCR amplification, indirectly lead to the situation of unstable product caused by unstable temperature. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of PCR amplification's micro -fluidic chip, can solve the problem that traditional PCR amplifies, the enzyme participating in the reaction is very sensitive to temperature, and the fluctuation of temperature can lead to unstable activity of polymerase, affect the speed of amplification and the quality of product, but the existing micro -fluidic chip can appear the situation of unstable temperature in amplification bin, avoid the problem of unstable enzyme during PCR amplification, and avoid the situation of unstable product caused by unstable temperature.

[0005] To achieve the above-mentioned purpose, provide a kind of PCR amplification's micro -fluidic chip, including micro -fluidic chip body, the upper portion of the micro -fluidic chip body is provided with temperature control system, the inside of the micro -fluidic chip body is provided with amplification bin;

[0006] The upper portion of the micro -fluidic chip body is provided with detection interface, the side of the detection interface is provided with cleaning liquid feed pipe, the side of the temperature control system is provided with feed box.

[0007] According to the micro -fluidic chip of PCR amplification, the upper portion of the feed box is fixedly connected with sample inlet, and the number of the sample inlet is four.

[0008] According to the micro -fluidic chip of PCR amplification, the side of the feed box is fixedly connected with micro -fluidic channel, and the shape of the micro -fluidic channel is spiral.

[0009] According to the microfluidic chip for PCR amplification, the amplification chamber is wrapped with a first coating layer on its side, and the first coating layer is wrapped with a second coating layer on its side.

[0010] According to the microfluidic chip for PCR amplification, the cleaning solution inlet tube is connected through the interior of the amplification chamber, and a first plug is threaded onto the top of the cleaning solution inlet tube.

[0011] According to the microfluidic chip for PCR amplification, a discharge tube is connected through the side of the amplification chamber, and a second plug is threaded to one end of the discharge tube.

[0012] According to the microfluidic chip for PCR amplification, a heating unit is connected inside the first coating layer, the first coating layer is made of quartz, and the second coating layer is made of silicone.

[0013] This utility model has the following beneficial effects:

[0014] 1. Compared with the prior art, this microfluidic chip for PCR amplification, by setting a first coating layer and a second coating layer, achieves stable temperature during PCR amplification and is not easily affected by external factors. This prevents the enzymes involved in the reaction from becoming unstable in activity, thus avoiding the problem of enzyme instability during PCR amplification and product instability caused by temperature instability.

[0015] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0016] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0017] Figure 1 This is a three-dimensional structural diagram of a microfluidic chip for PCR amplification according to the present invention.

[0018] Figure 2 This is a three-dimensional half-sectional view of a microfluidic chip for PCR amplification according to the present invention.

[0019] Figure 3 This is a top view of a microfluidic chip for PCR amplification according to the present invention.

[0020] Figure 4 This is a schematic diagram of the feed box and structure of a microfluidic chip for PCR amplification according to the present invention.

[0021] Legend:

[0022] 1, microfluidic chip body; 2, temperature control system; 3, amplification bin; 4, first wrapping layer; 5, feed box; 6, cleaning liquid feed pipe; 7, first plug; 8, detection interface; 9, heating unit; 10, discharge pipe; 11, second plug; 12, sample inlet; 13, microfluidic channel; 14, second wrapping layer. DETAILED DESCRIPTION

[0023] This part will describe the specific embodiments of the utility model in detail, the preferred embodiments of the utility model are shown in the drawings, the role of the drawings is to supplement the description of the text part with graphics, so that people can intuitively and visually understand each technical feature and the overall technical scheme of the utility model, but it cannot be understood as the limitation of the protection scope of the utility model.

[0024] Reference Figure 1-4 , the utility model embodiment a kind of PCR amplification microfluidic chip, it includes microfluidic chip body 1, the upper of microfluidic chip body 1 is provided with temperature control system 2, the inside of microfluidic chip body 1 is provided with amplification bin 3, the side of amplification bin 3 is wrapped with first wrapping layer 4, the side of first wrapping layer 4 is wrapped with second wrapping layer 14, the side of amplification bin 3 is connected with discharge pipe 10, one end of discharge pipe 10 is screw-connected with second plug 11, the inside of first wrapping layer 4 is connected with heating unit 9, the material of first wrapping layer 4 is quartz, the material of second wrapping layer 14 is silica gel;

[0025] The upper of microfluidic chip body 1 is provided with detection interface 8, the side of detection interface 8 is provided with cleaning liquid feed pipe 6, the side of temperature control system 2 is provided with feed box 5, the upper of feed box 5 is fixedly connected with sample inlet 12, the number of sample inlet 12 is four, the side of feed box 5 is fixedly connected with microfluidic channel 13, the shape of microfluidic channel 13 is spiral, cleaning liquid feed pipe 6 is connected in the inside of amplification bin 3, the upper of cleaning liquid feed pipe 6 is screw-connected with first plug 7.

[0026] The above structure, by setting the first wrapping layer 4 and the second wrapping layer 14, when the temperature inside the amplification chamber 3 needs to be stabilized and isolated, the temperature during PCR amplification can be stabilized under the action of the material of the first wrapping layer 4 and the second wrapping layer 14, and it is not easy to be affected by external factors, wherein the material of the first wrapping layer 4 is quartz, and the melting point of quartz is about 1650℃, which is much higher than the temperature range required by PCR (usually between 94℃ and 72℃). Therefore, quartz will not deform or melt in a high-temperature environment, and the thermal conductivity of quartz material is good, which can quickly and uniformly conduct heat. This can ensure that the temperature change (heating and cooling) during PCR amplification is fast and uniform, helping to optimize the amplification efficiency, and quartz has high corrosion resistance to most chemicals, so it can be used stably under various complex reaction conditions, avoiding inaccurate experimental results due to material reaction or pollution, and quartz is very hard and can resist long-term high-temperature cycling without aging or wear. The chip can have a longer service life during PCR amplification. Because of the stability and uniformity of quartz, it can provide a high-precision temperature control environment, reduce temperature fluctuations and errors, and thus improve the accuracy and reproducibility of amplification, wherein the material of the second wrapping layer 14 is silica gel, which has high thermal stability and is suitable for high temperatures (such as 94-98℃) and cooling stages during PCR thermal cycling. Even under extreme temperature conditions, silica gel can maintain its physical properties stable and will not melt or degrade. Silica gel has low thermal conductivity, which makes it effective in isolating external heat sources and maintaining the stability of the temperature inside the PCR reactor. It can prevent heat loss and avoid the impact of temperature fluctuations on reaction results, so that the enzyme involved in the reaction will not be unstable, thereby avoiding the problem of unstable enzyme during PCR amplification, and avoiding the problem of unstable product caused by unstable temperature.

[0027] By setting the feeding box 5 and the microchannel 13, when the sample and other reagents need to be added, they can be injected into the inside of the feeding box 5 through the four sample inlets 12, and the bottom of the feeding box 5 is connected with the microchannel 13, so that the sample and reagents in the feeding box 5 can enter the inside of the amplification chamber 3 through the microchannel 13, and under the spiral shape of the microchannel 13, the sample and reagents can be mixed, and then the mixture flows into the inside of the amplification chamber 3.

[0028] By setting the temperature control system 2 and the heating unit 9, when the temperature inside the amplification chamber 3 needs to be adjusted, the temperature inside the amplification chamber 3 can be controlled under the action of the temperature control system 2.

[0029] By setting the cleaning liquid feeding pipe 6 and the discharge pipe 10, when it is needed to clean the amplification bin 3, the first plug 7 can be first rotated to be detached, the cleaning liquid is added into the amplification bin 3, so that the amplification bin 3 can be cleaned, after cleaning, the second plug 11 is rotated to be separated, and then the cleaning liquid in the inside can be discharged.

[0030] Working principle: first, the sample and reagent needed by the experiment are injected into the inside of the feeding box 5 through the sample inlet 12, and under the action of the micro flow channel 13, the sample and reagent can be mixed, then the temperature control system 2 is controlled, so that the temperature in the amplification bin 3 can be controlled, and finally the PCR amplification is completed, when it is needed to clean the amplification bin 3, the first plug 7 can be first rotated to be detached, the cleaning liquid is added into the amplification bin 3, so that the amplification bin 3 can be cleaned, after cleaning, the second plug 11 is rotated to be separated, and then the cleaning liquid in the inside can be discharged.

[0031] The above embodiment of the utility model is described in detail in combination with the drawings, but the utility model is not limited to the above embodiment, within the knowledge range possessed by ordinary skilled in the art, various changes can be made without departing from the purpose of the utility model.

Claims

1. A microfluidic chip for PCR amplification, characterized by, Including microfluidic chip body (1), the top of microfluidic chip body (1) is provided with temperature control system (2), the inside of microfluidic chip body (1) is provided with amplification bin (3); The top of the microfluidic chip body (1) is provided with a detection interface (8), and the side of the detection interface (8) is provided with a cleaning liquid feeding pipe (6), and the side of the temperature control system (2) is provided with a feeding box (5).

2. The microfluidic chip for PCR amplification according to claim 1, wherein, The top of the feeding box (5) is fixedly connected with a sample inlet (12), and the number of the sample inlet (12) is four.

3. The microfluidic chip for PCR amplification according to claim 1, wherein, The side of the feeding box (5) is fixedly connected with a microfluidic channel (13), and the shape of the microfluidic channel (13) is spiral.

4. The microfluidic chip for PCR amplification according to claim 1, wherein, The side of the amplification bin (3) is wrapped with a first wrapping layer (4), and the side of the first wrapping layer (4) is wrapped with a second wrapping layer (14).

5. The microfluidic chip for PCR amplification according to claim 1, wherein, The cleaning liquid feeding pipe (6) is connected to the inside of the amplification bin (3), and the top of the cleaning liquid feeding pipe (6) is threadedly connected with a first plug (7).

6. The microfluidic chip for PCR amplification according to claim 1, wherein, The side of the amplification bin (3) is throughly connected with a discharge pipe (10), and one end of the discharge pipe (10) is threadedly connected with a second plug (11).

7. The microfluidic chip for PCR amplification according to claim 4, wherein, The inside of the first wrapping layer (4) is connected with a heating unit (9), the material of the first wrapping layer (4) is quartz, and the material of the second wrapping layer (14) is silica gel.

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