Fluorescence detection cassette based on microfluidic device
By designing a microfluidic fluorescence detection box that includes a light-shielding structure and an ultraviolet lamp, the problem of limited environment for fluorescence detection is solved, enabling convenient and accurate fluorescence detection, which is suitable for a variety of detection objects.
Patent Information
- Application Number
- CN202520369391.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-05
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2035-03-05
AI Technical Summary
Microfluidic fluorescence sensing arrays cannot be used in completely dark environments, resulting in inaccurate fluorescence detection results. Furthermore, the lack of portable devices affects the accuracy and reliability of the detection.
Design a fluorescence detection dark box based on a microfluidic device, comprising a dark box body, a light-shielding structure and an ultraviolet lamp to ensure a completely dark environment, and equipped with a photosensitive camera and USB data cable connection, and a portable power supply to realize fluorescence detection and imaging.
It enables rapid and accurate fluorescence detection under outdoor conditions, improving the convenience and accuracy of detection. It is suitable for a variety of detection objects and has a compact and portable structure.
Smart Images
Figure CN223857060U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the micro -fluidic fluorescence sensing array related technical field, concretely relates to a fluorescence detection dark box based on micro -fluidic device. BACKGROUND
[0002] Adopt micro -fluidic fluorescence sensing array technology, can synchronous detection multiple substances, but its key lies in the luminescent material needs under the ultraviolet excitation and completely dark environment can exhibit clear fluorescence. However, practical application faces two big challenges: one is environmental light interference, it is difficult to ensure absolute darkness, this will lead to fluorescence color display deviation, influence the accuracy of RGB value after mobile phone shooting, thereby reduce the detection precision. Two is the lack of portable equipment support when detecting on site, it is difficult to realize rapid and accurate detection, further influence the reliability of detection result.
[0003] In view of above problem, design a special device that can provide completely dark environment for micro -fluidic fluorescence sensing array detection is particularly urgent and important. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a fluorescence detection dark box based on micro -fluidic device to solve the problem that the fluorescence detection result is inaccurate when detecting by adopting micro -fluidic fluorescence sensing array technology.
[0005] In order to achieve the above object, the utility model adopts the following technical scheme:
[0006] A fluorescence detection dark box based on micro -fluidic device, including dark box body, the dark box body is combined by base plate, top panel, front baffle, rear baffle, left baffle and right baffle;The base plate front end is provided with a rotating shaft, and the rotating shaft is connected with a rotating drop plate, and the rotating drop plate is provided with a fluorescent micro -fluidic sensor annular array;The outer side of the front baffle is fixed with a light -shielding sample adding frame, and the light -shielding sample adding frame is provided with a sample dropping hole for dropping sample to the fluorescent micro -fluidic sensor annular array;The inner side of the front baffle is fixed with a light -shielding plate, and the light -shielding plate is provided with a notch that allows only one detection hole in the fluorescent micro -fluidic sensor annular array to be light -sensitive, and the notch is provided with an ultraviolet lamp, and the ultraviolet lamp is fixed on the left baffle or the right baffle;The top panel is provided with a camera mounting window, and the camera mounting window is provided with a light -sensitive camera.
[0007] Compared with the prior art, the utility model has the following beneficial effects:
[0008] The utility model discloses a fluorescent detection magazine based on micro -fluidic device has the fluorescent detection function, and the working wavelength is 365nm, and the detection ability is strong, and the compact structure is convenient for carrying, adopts USB data line connection portable power supply, and the long duration is worked, can detect and take the fluorescent photo of multiple detection objects simultaneously, guarantees the simultaneity and accuracy of detection analysis. BRIEF DESCRIPTION OF DRAWINGS
[0009] Fig. 1 It is the three-dimensional structure schematic diagram of the utility model.
[0010] Fig. 2 It is the overhead structure diagram of the utility model non -light camera.
[0011] Fig. 3 It is the side view structure diagram of the utility model without right baffle.
[0012] In the drawing: 1, back baffle;2, left baffle;3, power switch;4, ultraviolet lamp;5, light shield;6, rotary drop plate;7, light shield sample adding frame;8, front baffle;9, bearing;10, base plate;11, right baffle;12, shooting hole;13, light sensitive camera, 14-battery. DETAILED DESCRIPTION
[0013] The utility model will be explained further in combination with the drawings and specific implementation.
[0014] As Figs. 1-3 Shown, a kind of fluorescent detection magazine based on micro -fluidic device, including magazine body, which is combined by base plate 10, top panel 12, front baffle 8, back baffle 1, left baffle 2 and right baffle 11.The front end of base plate 10 is provided with rotating shaft 9, rotating shaft 9 is fixed with rotary drop plate 6, and rotary drop plate 6 is provided with fluorescent micro-fluidic sensor annular array;Front baffle 8 outside is fixed with light shield sample adding frame 7, and light shield sample adding frame 7 middle part is provided with sample drop hole for adding sample to fluorescent micro-fluidic sensor annular array;Front baffle 8 inside is fixed with light shield 5, and light shield 5 is provided with gap that only allows one detection hole of fluorescent micro-fluidic sensor annular array to be light sensitive, and the gap is provided with ultraviolet lamp 4 above, and ultraviolet lamp 4 is fixed on left baffle 2, and left baffle 2 outside is also fixed with power switch 3;Top panel 12 is provided with camera mounting window, and the camera mounting window is installed with light sensitive camera 13;Back baffle 1 inside is fixed with battery, and the battery is electrically connected with ultraviolet lamp 4 and light sensitive camera 13 by power switch 3, and back baffle 1 outside is provided with USB port and charging port, and the USB port is signal connected with light sensitive camera 13, and the charging port is electrically connected with battery 14.
[0015] The working process of the utility model is as follows:
[0016] 1. Add sample drop by drop: First, add the sample to be detected through the sample drop hole on the light-shielding sample holder, and carefully drop it into each detection hole of the fluorescent microfluidic sensor annular array on the rotating droplet plate.
[0017] 2. Rotate the droplet plate: Next, gently rotate the rotating droplet plate to ensure that each detection hole has sample, and send the rotating droplet plate into the dark box body.
[0018] 3. Connect the device: Turn on the power switch, then connect the device to a notebook computer or smartphone using the USB port to transmit signals.
[0019] 4. Turn on the ultraviolet lamp: At this time, the ultraviolet lamp will light up and emit ultraviolet light, which will shine on the rotating droplet plate. The light shield will block the excess ultraviolet light, ensuring that the ultraviolet light only accurately shines on the current detection hole.
[0020] 5. Detect one by one: By continuing to rotate the rotating droplet plate, each detection hole will be exposed to ultraviolet light one by one, so that each detection hole can be detected.
[0021] 6. Observe and record: During the detection process, the staff can observe whether the detection hole is rotated in place through the photosensitive camera, and check whether the sample emits fluorescence. Once fluorescence is observed, take a photo immediately for subsequent experimental analysis.
[0022] 7. End operation: When all detection holes have been photographed, press the power switch again to turn off the ultraviolet lamp and photosensitive camera, and end the entire detection process.
[0023] The fluorescent microfluidic sensor annular array design adopted by the utility model is flexible and variable, and is not limited to specific detection objects. For example, when detecting heavy metal ions in water, the advanced fluorescent microfluidic sensor array developed by the research team of Professor Jiang Changlong of the Energy Materials and Device Manufacturing Research Department of the Institute of Solid State Physics, Hefei Material Science Research Institute, Chinese Academy of Sciences can be used. If it needs to be applied to other fields, only the corresponding fluorescent probe on the sensor needs to be replaced, and the detection of different target objects can be easily realized. As for the parts not described in detail (such as image information transmission processing, battery charging rectification, etc.), components or methods with similar functions in existing technology can be used to achieve them, and they will not be described again.
[0024] To sum up, the utility model innovatively combines the fluorescent microfluidic sensor and the detection dark box, and ingeniously constructs a dark environment specially designed for fluorescence detection. This innovative design not only solves the problem of limited fluorescence detection environment in the prior art, but also greatly improves the convenience of outdoor operation. Through the perfect combination of the two, it is ensured that even under outdoor conditions, fluorescence detection can be quickly and accurately performed, greatly enhancing the timeliness and accuracy of detection. For the majority of scientific researchers, the utility model is undoubtedly a powerful assistant for carrying out related research, and its excellent practical application value is self-evident, which injects strong impetus for promoting the scientific research process.
Claims
1. A microfluidic device based fluorescence detection cartridge, characterized in that, The cartridge body is combined by a base plate (10), a top panel (12), a front baffle (8), a rear baffle (1), a left baffle (2) and a right baffle (11); the base plate (10) is provided with a rotating shaft (9) at the front end, the rotating shaft (9) is fixed with a rotating drop plate (6), the rotating drop plate (6) is provided with a fluorescent microfluidic sensor annular array; the front baffle (8) is fixed with a light-shielding sample adding frame (7) outside, the light-shielding sample adding frame (7) is provided with a sample dropping hole in the middle for dropping sample to the fluorescent microfluidic sensor annular array; the front baffle (8) is fixed with a light-shielding plate (5) inside, the light-shielding plate (5) is provided with a notch allowing only one detection hole of the fluorescent microfluidic sensor annular array to be light-sensitive, the notch is provided with an ultraviolet lamp (4) above, the ultraviolet lamp (4) is fixed on the left baffle (2) or the right baffle (11); the top panel (12) is provided with a camera mounting window, the camera mounting window is mounted with a light-sensitive camera (13).
2. The microfluidic device-based fluorescence detection cartridge of claim 1, wherein, The rear baffle (1) is further fixed with a battery inside, the battery is electrically connected with the ultraviolet lamp (4) and the light-sensitive camera (13).
3. The microfluidic device-based fluorescence detection cartridge of claim 2, wherein, The rear baffle (1) is further provided with a USB port and a charging port, the USB port is signal-connected with the light-sensitive camera (13), and the charging port is electrically connected with the battery.
4. The microfluidic device-based fluorescence detection cartridge of claim 1, wherein, The ultraviolet lamp (4) is fixed on the left baffle (2), and the left baffle (2) is further provided with a power switch (3) outside.