Water environment DNA pressing type in-situ filtering and storing device

Abstract

The utility model discloses a water environment DNA push-type in-situ filtration preservation device, which comprises a sample loading pipe and a dry ice heat preservation barrel for low-temperature sample storage, the inner surface of the sample loading pipe is provided with a sample loading cavity for water sample storage, the inner cavity of the sample loading cavity is provided with a plunger handle in a sliding manner, and the bottom of the surface of the plunger handle is sleeved with a rubber pressure plug; a flow dividing bin is arranged at the bottom of the sample loading pipe, a filter membrane placing bin is in threaded connection with the bottom of an inner cavity of the flow dividing bin, the flow dividing bin and the filter membrane placing bin are used in cooperation to form a filter cavity used for filtering water extracted by the sample loading pipe, and a filter membrane piece is laid in the inner cavity of the filter membrane placing bin. According to the device, through a compact design and a simple and convenient operation process, efficient collection and filtration of a water body can be completed in real time at a sampling point, and the integrity of DNA is kept to the maximum extent through on-site low-temperature storage, so that more reliable and convenient technical support is provided for aquatic organism community information investigation.

Description

Technical Field

[0001] This utility model belongs to the field of aquatic environment DNA collection technology, and in particular relates to an aquatic environment DNA press-type in-situ filtration and preservation device. Background Technology

[0002] With the rapid development of environmental DNA (eDNA) technology, the synergy between environmental protection and technological innovation provides a powerful approach to solving ecological and environmental problems. In particular, eDNA macrobarcoding technology can perform molecular identification of multiple species in aquatic communities, providing an economical and efficient survey method. This has greatly promoted the development of water quality assessment, ecosystem health monitoring, and environmental pollution monitoring, and shows broad prospects in biodiversity research and environmental monitoring.

[0003] Traditional aquatic community information surveys rely on morphological identification methods, which are often invasive and inefficient at identifying small or elusive species. In contrast, environmental DNA technology stands out due to its non-invasiveness and high sensitivity, enabling the extraction and analysis of genetic material from water samples without directly capturing or disturbing target organisms, thus providing rapid and accurate information on the composition of aquatic communities. However, environmental DNA suffers from high degradation rates and complex migration patterns in the natural environment, posing higher requirements for sample collection and preservation.

[0004] Currently, environmental DNA collection mainly employs two methods: water sample return and in-situ filtration. While water sample return is simple to operate, environmental DNA degradation during transportation and processing is unavoidable, leading to increased errors in the results. In-situ filtration, on the other hand, can filter water immediately at the sampling point, reducing the risk of DNA degradation. However, traditional in-situ filtration equipment is bulky, lacks portability, has low independent operation efficiency, and is difficult to control for cross-contamination within the equipment, limiting its application in field operations.

[0005] Furthermore, traditional methods of collecting aquatic DNA samples typically require bringing the samples back to the laboratory for cryopreservation and subsequent processing. This not only increases time costs but may also lead to time lag issues with the samples relative to the actual environment. Although the emergence of new sampling equipment has addressed the issue of easy degradation of DNA in field water to some extent, these devices often consist of collection modules, filtration modules, power modules (such as power supply), and cryopreservation modules. The overall structure is quite complex, requiring operators to carry bulky equipment and sometimes even needing assistance from colleagues to complete sample collection, thus increasing the difficulty and complexity of field operations.

[0006] Therefore, effectively reducing the degradation of DNA samples from water bodies in the field while improving the operability and portability of environmental DNA collection equipment has become a key issue that urgently needs to be addressed in this field. To address this challenge, this invention proposes a press-type in-situ filtration and preservation device for aquatic environmental DNA. Through its compact design and simple operating procedure, it aims to achieve efficient collection, immediate filtration, and on-site cryogenic preservation of water samples, thereby maximizing the integrity and representativeness of environmental DNA and providing more reliable technical support for aquatic biological community information surveys. Utility Model Content

[0007] The purpose of this invention is to provide a water environment DNA press-type in-situ filtration and preservation device to solve the technical problems mentioned in the background art, such as easy DNA degradation, poor equipment portability, difficulty in independent operation, and high risk of cross-contamination.

[0008] To achieve the above objectives, the specific technical solution of this utility model is as follows: A water environment DNA press-type in-situ filtration and preservation device includes a sample loading tube and a dry ice insulated bucket for low-temperature sample storage. The inner surface of the sample loading tube is provided with a sample loading cavity for water sample storage. A plunger handle is slidably arranged in the inner cavity of the sample loading cavity, and a rubber pressure plug is fitted on the bottom of the surface of the plunger handle.

[0009] The bottom of the sample tube is provided with a diversion chamber, and the bottom of the inner cavity of the diversion chamber is threadedly connected to a filter membrane placement chamber. The diversion chamber and the filter membrane placement chamber work together to form a filtration chamber for filtering the water extracted by the sample tube. The inner cavity of the filter membrane placement chamber is lined with a filter membrane.

[0010] The dry ice insulated container contains dry ice and EP test tubes. After the EP test tubes are used to take samples, they are placed inside the dry ice insulated container and covered by dry ice.

[0011] Preferably, a push handle is connected to the end of the outer surface of the sample tube, and an injection tube is connected to the bottom of the sample tube.

[0012] Preferably, a flow guiding cavity is formed on the inner surface of the flow diversion chamber, and a flow diversion orifice plate is connected to the middle of the inner cavity of the flow guiding cavity, wherein an airtight rubber ring is fitted at the bottom of the inner cavity of the flow guiding cavity.

[0013] Preferably, the bottom of the filter membrane placement chamber is connected to a wastewater outlet pipe for discharging the filtered wastewater.

[0014] Preferably, a lid is threaded onto the end of the outer surface of the dry ice insulated bucket, which can seal the inner cavity of the dry ice insulated bucket, and a storage groove is provided at one end of the outer surface of the dry ice insulated bucket.

[0015] Preferably, the bottom of the dry ice insulated bucket is provided with a purified water storage tank, wherein a liquid pressure pipe is provided at the bottom of the inner cavity of the purified water storage tank, and multiple liquid outlet holes are opened on the right side of the surface of the liquid pressure pipe.

[0016] A push rod is installed through the inner cavity of the liquid-pressing tube, and a rubber stopper is fitted at one end of the push rod inside the inner cavity of the liquid-pressing tube.

[0017] Preferably, a connecting pipe is connected to the right side of the hydraulic tube, the connecting pipe extends to the outside of the hydraulic tube, and the tail end of the connecting pipe is connected to a limiting screw cylinder by a thread.

[0018] Preferably, the surface of the filter membrane placement chamber is further provided with a limiting and anti-detachment component, which includes a push-connecting ring plate. The outer surface of the filter membrane placement chamber is provided with an installation groove for sliding assembly of the push-connecting ring plate, and the surface of the push-connecting ring plate is provided with multiple anti-void grooves.

[0019] Preferably, the outer surface of the push ring plate is fitted with an elastic rubber ring, which is made of elastic material, and the inner surface of the elastic rubber ring is connected with a plurality of plug posts.

[0020] Preferably, the outer surface of the push-fit ring plate is connected to a plurality of push-fit blocks, and the surface of the elastic rubber ring is provided with a mating groove for use with the push-fit blocks.

[0021] This utility model provides a water environment DNA compression-type in-situ filtration and preservation device with the following advantages:

[0022] This invention relates to a press-type in-situ DNA filtration and preservation device for aquatic environments. The quantitative water extraction module allows for flexible extraction of a specific amount of water from the environment. During filtration, pressing the plunger handle increases the water pressure in the sample tube, improving filtration efficiency. No additional power source is required, meeting the portability requirements for DNA sampling in the field. The filtration module employs a separate upper and lower structure for the diversion chamber and filter membrane placement chamber, coupled with an airtight rubber ring, providing faster filtration speeds and more convenient filter membrane placement while maintaining portability. The filter membrane preservation module, combined with a high-density cryogenic medium, enables long-term low-temperature storage in a small volume. Compared to traditional methods of preserving water samples in the field or using portable refrigerators, this provides smaller volume, lower temperature, and more continuous sample storage conditions, reducing the degradation rate of DNA in the sample and ensuring the timeliness of the DNA sample. Overall, this invention significantly improves the operability, portability, and timeliness of DNA sample filtration and preservation in aquatic environments. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0025] Figure 2 This is a schematic diagram of the overall exploded structure of this utility model;

[0026] Figure 3 This is a partial cross-sectional view of the purified water storage tank of this utility model.

[0027] Figure 4 This is a schematic front sectional view of the upper sample tube structure of this utility model;

[0028] Figure 5 This is a front sectional view of the diversion chamber and filter membrane placement chamber structure of this utility model;

[0029] Figure 6 This is an exploded view of the upper sample tube structure of this utility model;

[0030] Figure 7 This is an exploded view of the sample loading tube, the diversion chamber, and the filter membrane placement chamber of this utility model.

[0031] Figure 8 This is an exploded view of a portion of the push-fit ring plate and rubber ring of this utility model.

[0032] Explanation of markings in the diagram: 100, Sample loading tube; 102, Sample loading chamber; 103, Push handle; 104, Injection tube; 200, Plunger handle; 201, Rubber pressure plug; 300, Flow divider; 301, Flow guide chamber; 302, Flow divider orifice plate; 303, Airtight rubber ring; 400, Filter membrane placement chamber; 401, Filter membrane element; 402, Waste liquid outlet pipe; 500, Dry ice insulated container; 501, E P test tube; 502, bucket lid; 503, storage tank; 600, purified water storage tank; 610, liquid pressure pipe; 611, liquid outlet; 620, push rod; 630, rubber stopper; 640, connecting pipe; 641, limiting screw; 700, push ring plate; 701, mounting groove; 702, anti-vacuum groove; 710, push block; 720, elastic rubber ring; 721, mating groove; 730, plug-in post. Detailed Implementation

[0033] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0034] In the description of the embodiments of this utility model, it should be understood that the terms "length", "vertical", "horizontal", "top", "bottom", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this utility model.

[0035] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of embodiments of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0036] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0037] The following disclosure provides many different implementations or examples for different structures of the embodiments of the present invention. To simplify the disclosure of the embodiments of the present invention, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the embodiments of the present invention. Furthermore, reference numerals and / or reference letters may be repeated in different examples of the embodiments of the present invention; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various implementations and / or arrangements discussed.

[0038] To better understand the purpose, structure, and function of this utility model, the following description, in conjunction with the accompanying drawings, provides a more detailed account of a water environment DNA press-type in-situ filtration and preservation device.

[0039] like Figures 1-2As shown, this utility model discloses a water environment DNA press-type in-situ filtration and preservation device, which includes a positioning water intake module, a filtration module, and a filter membrane preservation module.

[0040] The positioning water extraction module consists of a sample tube 100, a sample chamber 102, a push handle 103, an injection tube 104, a plunger handle 200, and a rubber pressure plug 201. The sample tube 100 has a sample chamber 102 for storing water samples on its inner surface. To facilitate pressing the plunger handle 200, a push handle 103 is connected to the end of the outer surface of the sample tube 100. When pressing the plunger handle 200, the index and middle fingers abut against the push handle 103, and the thumb presses the end of the plunger handle 200, which makes it convenient for the user to press and pull the plunger handle 200 to extract water samples.

[0041] To collect water samples and deliver the water samples from the upper sample chamber 102 to the distribution chamber 300, the bottom of the upper sample tube 100 is connected to an injection tube 104. The injection tube 104 and the inner cavity of the upper sample chamber 102 are in a connected state, so that water samples can be extracted through the injection tube 104. Under the function of the injection tube 104, not only can larger impurities be prevented from entering during sampling, but the upper sample tube 100 and the distribution chamber 300 can also be connected conveniently.

[0042] The sample loading chamber 102 has a plunger handle 200 slidably mounted inside, and a rubber pressure plug 201 is fitted on the bottom of the surface of the plunger handle 200. The rubber pressure plug 201 works in conjunction with the inner cavity of the sample loading chamber 102. When the sample loading tube 100 takes water, the plunger handle 200 is pulled to conduct the force to the rubber pressure plug 201, drawing water into the sample loading chamber 102. When the water sample is discharged from the sample loading chamber 102, the plunger handle 200 is pushed in the opposite direction, and the rubber pressure plug 201 increases the water pressure in the sample loading chamber 102, squeezing the water from the injection tube 104 into the diversion chamber 300 in the subsequent filtration module, thereby improving the filtration efficiency of the water in the field.

[0043] The sample tube 100 has graduation lines on its surface, which can meet the requirements for collecting water within 1m of the surface. Depending on the expected sample volume, the specifications of the sample tube 100 can be changed or the environmental water can be extracted multiple times.

[0044] By pulling the plunger handle 200, the user generates negative pressure in the sample tube 100 through the sealing effect between the rubber pressure plug 201 and the sample chamber 102, thereby extracting a quantitative water sample through the injection tube 104. The water volume can be precisely controlled according to the scale lines on the sample tube 100.

[0045] The filtration module consists of a flow distribution chamber 300, a flow guiding cavity 301, a flow distribution orifice plate 302, an airtight rubber ring 303, a filter membrane placement chamber 400, a filter membrane element 401, and a waste liquid outlet pipe 402, etc.; the filtration module adopts an upper and lower separated structure design.

[0046] The bottom of the sample tube 100 is provided with a diversion chamber 300, the end of the diversion chamber 300 is the water inlet, and the inner surface of the diversion chamber 300 is provided with a flow guide cavity 301. The middle of the inner cavity of the flow guide cavity 301 is connected to a diversion orifice plate 302. The flow guide cavity 301 and the airtight rubber ring 303 form a uniform water outlet space in the diversion chamber 300, and the water sample is sent into the filter membrane placement chamber 400 through the diversion chamber 300. The bottom of the inner cavity of the flow guide cavity 301 is fitted with an airtight rubber ring 303, which is used to increase the airtightness of the internal space when the diversion chamber 300 and the filter membrane placement chamber 400 are connected.

[0047] Specifically, a filter membrane placement chamber 400 is threadedly connected to the bottom of the inner cavity of the split chamber 300. The split chamber 300 and the filter membrane placement chamber 400 work together to form a filtration chamber for filtering the water extracted by the sample tube 100, so as to collect the DNA carrier particles. The inner cavity of the filter membrane placement chamber 400 is lined with a filter membrane 401 for filtering and collecting water samples. The split chamber 300 and the filter membrane placement chamber 400 adopt an upper and lower separate design. The high-pressure water flow from the injection tube 104 passes through the split chamber 300. The water is fed into the distribution chamber 300 through the inlet at the top of the flow chamber 300, and then evenly enters the filter membrane placement chamber 400 through the guide cavity 301 and the distribution plate 302. Under the compression of the upper and lower separation structure, the distribution plate 302 forms an airtight space in the middle, and the water pressure acts evenly on the surface of the filter membrane 401, driving the water filter membrane 401 to flow out from the waste liquid outlet pipe 402. Larger DNA carrier particles are retained on the surface of the filter membrane 401, thereby achieving efficient filtration of water samples and collecting water DNA. After completing the in-situ filtration of the environmental water, the distribution chamber 300 and the filter membrane placement chamber 400 of the filtration module are opened, the filter membrane 401 with adsorbed environmental DNA is taken out, and it is quickly placed into the EP test tube 501 and transferred to the dry ice insulated container 500 at -78℃ for storage.

[0048] The bottom of the filter membrane placement chamber 400 is connected to a waste liquid outlet pipe 402 for discharging waste liquid after filtration. Thus, a complete liquid outlet channel is formed in the diversion chamber 300 and the filter membrane placement chamber 400 in conjunction with the guide cavity 301 and the diversion orifice plate 302. After filtration by the filter membrane element 401, the water can be discharged through the waste liquid outlet pipe 402. In this way, with the cooperation of the positioning water sampling module and the filtration module, the water sample is filtered, thereby collecting DNA carrier particles.

[0049] Finally, the plunger handle 200 is pushed in the opposite direction, and the water sample in the sample loading chamber 102 is injected into the split chamber 300 through the injection tube 104 under pressure. The water flow is evenly distributed under the action of the guide chamber 301 and the split orifice plate 302, and is filtered through the filter membrane 401. The larger DNA carrier particles are retained on the surface of the filter membrane 401, while the filtered liquid is discharged from the waste liquid outlet pipe 402.

[0050] To prevent the filter membrane 401 from shaking arbitrarily during water filtration, a limiting and anti-detachment component is provided on the surface of the filter membrane placement chamber 400. The limiting and anti-detachment component includes a push-connecting ring plate 700, wherein the outer surface of the filter membrane placement chamber 400 is provided with an installation groove 701 for sliding assembly of the push-connecting ring plate 700.

[0051] The surface of the push ring plate 700 is provided with multiple anti-void grooves 702. The anti-void grooves 702 are used in conjunction with the insertion post 730. Since the insertion post 730 extends through the push ring plate 700 into the inner cavity of the filter membrane placement chamber 400, the connection between the insertion post 730 and the filter membrane placement chamber 400 is a sealed sliding contact. Therefore, when the push ring plate 700 is rotated, and the elastic rubber ring 720 is in a non-rotating state, it will inevitably be blocked by the insertion post 730. Therefore, the anti-void grooves 702 are provided, and the outer surface of the insertion post 730 is set in the inner cavity of the anti-void groove 702, so that the push ring plate 700 has a certain rotation space.

[0052] An elastic rubber ring 720 is fitted onto the outer surface of the push-fit ring plate 700. The elastic rubber ring 720 is made of elastic material. Multiple insertion posts 730 are connected to the inner surface of the elastic rubber ring 720. Multiple push-fit blocks 710 are connected to the outer surface of the push-fit ring plate 700. A mating groove 721 is formed on the surface of the elastic rubber ring 720 to cooperate with the push-fit blocks 710. The surfaces of the push-fit ring plate 700 and the elastic rubber ring 720 slide in contact. In the initial state, the push-fit blocks 710 extend into the mating groove 721, allowing the insertion posts 730 to enter the filter membrane placement chamber. When the filter membrane element 401 is abutted by the 400, and the filter membrane element 401 needs to be removed, it is only necessary to limit the anti-gaps groove 702, and then rotate the push block 710 so that the push block 710 passes through the mating groove 721. The elastic rubber ring 720 expands outward under the pressure of the push block 710, and moves the insertion post 730 outward, leaving space for the filter membrane element 401 to be taken out, and releasing the limitation of the insertion post 730 on the filter membrane element 401. After the push block 710 is rotated in the opposite direction and enters the mating groove 721, the insertion post 730 limits and fixes the filter membrane element 401 again.

[0053] The filter membrane preservation module consists of a dry ice insulated container 500, an EP test tube 501, a container lid 502, and a storage slot 503. The sample loading tube 100 is placed in the storage slot 503 on the surface of the dry ice insulated container 500. The dry ice insulated container 500 contains dry ice and the EP test tube 501. After sampling, the EP test tube 501 is placed in the dry ice insulated container 500 and covered by dry ice to preserve the sample collected in the EP test tube 501 at low temperature.

[0054] A lid 502 is threaded onto the end of the outer surface of the dry ice insulated bucket 500. The lid 502 can seal the inner cavity of the dry ice insulated bucket 500. A storage slot 503 is opened at one end of the outer surface of the dry ice insulated bucket 500 for positioning and placing the water dispensing module and the filter module. The surface of the storage slot 503 is movably connected to a door through a hinge, forming a sealed space inside the storage slot 503 to effectively store the positioning water dispensing module and the filter module and prevent the water dispensing module and the filter module from being lost.

[0055] After filtration is complete, open the split chamber 300 and the filter membrane placement chamber 400, carefully remove the filter membrane 401 containing DNA fragments using pre-washed tweezers, and quickly place it into the EP test tube 501. Then transfer it to the dry ice insulated container 500 at -78°C for low-temperature storage to prevent DNA degradation.

[0056] To clean the positioning water intake module and the filter module and avoid cross-contact of the samples, a purified water storage tank 600 is set at the bottom of the dry ice insulated tank 500. A liquid pressure pipe 610 is set at the bottom of the inner cavity of the purified water storage tank 600, and multiple liquid outlet holes 611 are opened on the right side of the surface of the liquid pressure pipe 610, which can send pure water in the purified water storage tank 600 into the liquid pressure pipe 610.

[0057] The left end of the purified water storage tank 600 is connected to a pure water inlet pipe, which can send pure water into the purified water storage tank 600 for storage.

[0058] A push rod 620 is installed through the inner cavity of the pressure pipe 610. The push-pull end of the push rod 620 extends through the pressure pipe 610 to the outside of the purified water storage tank 600. The connection between the push rod 620 and the pressure pipe 610 is in sealed sliding contact. A rubber stopper 630 is fitted at one end of the push rod 620 and inside the pressure pipe 610. The rubber stopper 630 is in sealed sliding contact with the inner cavity of the pressure pipe 610. Under the action of the push rod 620, purified water in the purified water storage tank 600 can be quickly drawn into the pressure pipe 610. When the push rod 620 is pressed inward, the water can be sent out through the rubber stopper 630 to clean the positioning water intake module and the filter module.

[0059] The right side of the liquid-pressing tube 610 is connected to a connecting tube 640, which extends to the outside of the liquid-pressing tube 610. The connecting tube 640 consists of a flexible hose and a fixed tube. The tail end of the surface of the connecting tube 640 is connected to a limiting screw 641 by a thread. The surface of the injection tube 104 is also threaded. Thus, after connecting the connecting tube 640 and the injection tube 104, one end of the limiting screw 641 is screwed into the injection tube 104, connecting the sample loading tube 100 to the liquid-pressing tube 610. Under the action of the push rod 620 or the plunger handle 200, the purified water in the purified water storage tank 600 can be drawn into the sample loading chamber 102 for cleaning.

[0060] The working principle of a press-type in-situ DNA filtration and preservation device for aquatic environments is as follows: Before collecting water samples, sterile gloves must be worn and contact with contaminants avoided. Before filtration, the quantitative water extraction module is rinsed, and water is extracted quantitatively according to the graduations on the sample tube 100. Different capacity sample tubes 100 can be selected according to sample requirements, or multiple samples can be taken to reach the expected sample volume. During filtration, the plunger handle 200 is pressed, and the water in the sample tube 100 is injected into the filtration module under pressure. After filtration through the filter membrane 401, the aqueous phase flows out from the waste liquid outlet pipe 402 below, and DNA fragments in the water are retained on the surface of the filter membrane 401. After filtration, the filter membrane 401 containing the adsorbed DNA fragments is quickly placed into an EP test tube 501 using rinsed tweezers and transferred to a dry ice insulated container 500 at -78℃ for preservation. This invention has a simple structure and significant effect, providing a highly portable, time-efficient, and low-degradation method for DNA collection in aquatic environments in the field.

[0061] Throughout the process, the limiting and anti-detachment components ensure the stability of the filter membrane 401 during filtration, while the purified water storage tank 600 and its internal liquid-pressing pipe 610, push rod 620 and other structures provide effective cleaning of the positioning water intake module and the filtration module, avoiding cross-contamination between samples, and realizing efficient, portable and low-degradation collection of DNA samples in the field water environment.

[0062] It is understood that this utility model has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of this utility model. Furthermore, under the teachings of this utility model, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of this utility model.

Claims

1. A pressure-type in-situ filtration and preservation device for DNA in aquatic environments, characterized in that: It includes a sample loading tube (100) and a dry ice insulated container (500) for low-temperature storage of samples. The inner surface of the sample loading tube (100) is provided with a sample loading cavity (102) for storing water samples. A plunger handle (200) is slidably provided in the inner cavity of the sample loading cavity (102), and a rubber pressure plug (201) is fitted on the bottom of the surface of the plunger handle (200). The bottom of the sample tube (100) is provided with a diversion chamber (300), and the bottom of the inner cavity of the diversion chamber (300) is threadedly connected to a filter membrane placement chamber (400). The diversion chamber (300) and the filter membrane placement chamber (400) are used together to form a filter cavity for filtering the water extracted by the sample tube (100). The inner cavity of the filter membrane placement chamber (400) is lined with a filter membrane element (401). The dry ice insulated container (500) contains dry ice and EP test tubes (501), and the EP test tubes (501) are placed inside the dry ice insulated container (500) and covered by dry ice after sampling.

2. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 1, characterized in that: The sample tube (100) has a push handle (103) connected to the end of its outer surface, and an injection tube (104) connected to the bottom of its bottom.

3. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 2, characterized in that: The inner surface of the diversion chamber (300) is provided with a flow guide cavity (301), and a flow guide plate (302) is connected to the middle of the inner cavity of the flow guide cavity (301). An airtight rubber ring (303) is fitted at the bottom of the inner cavity of the flow guide cavity (301).

4. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 3, characterized in that: The bottom of the filter membrane placement chamber (400) is connected to a waste liquid outlet pipe (402) for discharging the filtered waste liquid.

5. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 4, characterized in that: The dry ice insulated bucket (500) has a bucket lid (502) threaded onto the end of its outer surface. The bucket lid (502) can seal the inner cavity of the dry ice insulated bucket (500), and a storage groove (503) is provided at one end of the outer surface of the dry ice insulated bucket (500).

6. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 5, characterized in that: The bottom of the dry ice insulated bucket (500) is provided with a purified water storage tank (600), wherein a liquid pressure pipe (610) is provided at the bottom of the inner cavity of the purified water storage tank (600), and multiple liquid outlet holes (611) are opened on the right side of the surface of the liquid pressure pipe (610). A push rod (620) is provided through the inner cavity of the liquid-pressing tube (610), and a rubber stopper (630) is fitted at one end of the push rod (620) and inside the liquid-pressing tube (610).

7. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 6, characterized in that: The right side of the hydraulic tube (610) is connected to a connecting tube (640), which extends to the outside of the hydraulic tube (610). The tail end of the surface of the connecting tube (640) is connected to a limiting screw cylinder (641) by a thread.

8. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 7, characterized in that: The surface of the filter membrane placement chamber (400) is also provided with a limiting anti-detachment component, which includes a push-connecting ring plate (700). The outer surface of the filter membrane placement chamber (400) is provided with an installation groove (701) for sliding assembly of the push-connecting ring plate (700), and the surface of the push-connecting ring plate (700) is provided with a plurality of anti-void grooves (702).

9. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 8, characterized in that: The outer surface of the push ring plate (700) is fitted with an elastic rubber ring (720), which is made of elastic material. The inner surface of the elastic rubber ring (720) is connected with a plurality of plug posts (730).

10. The aquatic environment DNA press-type in-situ filtration and preservation device according to claim 9, characterized in that: The outer surface of the push ring plate (700) is connected to a plurality of push blocks (710), and the surface of the elastic rubber ring (720) is provided with a mating groove (721) for use with the push blocks (710).

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