Adhesive biological sample collecting tube

Abstract

The utility model relates to an adhesive biological sample collecting tube which comprises a tube body and a tube cover, one end of the tube body is closed, the other end of the tube body is open, the tube cover covers the open end of the tube body, an adsorption layer used for adhering a biological sample is arranged on the inner side of the tube cover, and a ruler used for marking the position of the biological sample is arranged on the tube cover. The arrangement position of the ruler is matched with the arrangement position of the adsorption layer, and the adsorption layer is located in the tube body when the tube cover covers the tube body. According to the utility model, an obtained sample can be accurately positioned under a microscope, a target tissue can be visually and secondarily collected, a target experiment scheme can be directly implemented in a collection tube, unused samples do not need to be taken out in advance, the cell collection efficiency is greatly improved, and compared with other existing cell tissue collection methods, the cell collection efficiency is greatly improved. And the loss rate of the cell sample is reduced by 50%.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical technology, specifically relating to an adhesive biological sample collection tube. Background Technology

[0002] Organisms are composed of various cell types that are interconnected. Previously, studies using whole tissue samples (mixed samples) did not accurately reflect the biological information of individual cell types. Tissue sections, with advancements in microscopy and immunoassays, have addressed the need for obtaining some tissue samples at a single level. However, this goal remains difficult to achieve due to mechanical and procedural limitations in cell manipulation (e.g., cell collection or storage) and the considerable time required. In particular, isolating single or rare cells or microscopic tissue from tiny samples requires researchers to capture individual cells under a microscope; therefore, ensuring that the sample container contains only one cell for further testing is crucial.

[0003] Currently, there are gravity methods and blow-fleck methods available both domestically and internationally. These methods rely on gravity or external elastic force to obtain tiny tissues under a microscope. However, the tissue area is usually between 500 and 5000 square micrometers, or it may be a single cell or even a chromosome. Because the sample is so small and invisible to the naked eye, it is usually impossible to accurately determine whether the target product has been obtained, or the sample may be lost during the processing.

[0004] In summary, there is an urgent need to provide an adhesive biological sample collection tube that can improve cell collection efficiency and reduce sample loss rate. Utility Model Content

[0005] The purpose of this invention is to provide an adhesive biological sample collection tube that can improve cell collection efficiency and reduce sample loss rate.

[0006] The above objective is achieved through the following technical solution: an adhesive biological sample collection tube, comprising a tube body and a tube cap, wherein one end of the tube body is closed and the other end is open, the tube cap is fitted onto the open end of the tube body, an adsorption layer for adhering biological samples is provided on the inner side of the tube cap, and a scale for marking the position of the biological samples is provided on the tube cap, the setting position of the scale matching the setting position of the adsorption layer, and the adsorption layer being located inside the tube body when the tube cap is fitted onto the tube body.

[0007] It should be understood that the adsorption layer has a certain degree of transparency, allowing the scale markings indicating the position of the biological sample to be seen through it. In specific applications, the required micro-tissue adheres to the surface of the adsorption layer under a microscope. By setting a precision scale at the position of the adsorption layer, the acquired sample can be accurately positioned under a microscope, and the target tissue can be visualized for secondary collection. The collection tube design of this utility model eliminates the need to remove unused samples beforehand, allowing the target experimental protocol to be implemented directly in this collection tube, greatly improving cell collection efficiency and reducing cell sample loss rate by 50% compared to other existing cell and tissue collection methods.

[0008] To better record the position, the scale is coded. The scale and the code are laser-etched in the center of the tube cap, and the thickness of the engraving line can be as narrow as 10-15nm.

[0009] A further technical solution is that the inner wall of the tube cap is provided with an accommodating space, and the adsorption layer is filled in the accommodating space.

[0010] A further technical solution is that the inner wall of the pipe cover is provided with annular ribs, and the annular ribs form the accommodating space.

[0011] A further technical solution is that the center of the scale coincides with the center of the accommodating space.

[0012] A further technical solution is that the scale is disposed on the inner wall of the tube cap; or the tube cap is made of a material with predetermined transparency, and the scale is disposed on the outer wall of the tube cap.

[0013] A further technical solution is that the adsorption layer is a photocurable resin, an organosilicon resin, or a silicone.

[0014] A further technical solution is that one side of the open end of the tube is connected to the tube cap via a flexible connector, and the other side is fastened to the tube cap. Preferably, the tube body, tube cap, and flexible connector are made of plastic and integrally injection molded.

[0015] A further technical solution is that the open end of the tube body is provided with a wedge protruding from the outer surface of the tube body, and the inner wall of the tube cap is provided with a limiting block, the limiting block having a limiting groove. When the tube body and the tube cap are fastened together, the limiting block is fastened onto the wedge, and the wedge is embedded in the limiting groove. With this configuration, when the tube cap is placed on the tube body, the lower wall of the tube cap and the upper end face of the tube body are in close contact. After the limiting block is fastened onto the wedge, it provides a certain tension, ensuring a tight fit between the lower wall of the tube cap and the upper end face of the tube body. This not only results in a simple structure and good sealing performance, but also prevents the tube cap from falling off, preventing sample loss and improving sample safety.

[0016] Compared with existing technologies, this invention can accurately locate the acquired samples under a microscope and visualize the secondary collection of target tissues. It can directly collect the target experimental plan in the collection tube without removing unused samples in advance, which greatly improves the cell collection efficiency and reduces the cell sample loss rate by 50% compared with other existing cell and tissue collection methods. Attached Figure Description

[0017] The accompanying drawings, which form part of this utility model, are used to provide a further understanding of this utility model. The illustrative embodiments of this utility model and their descriptions are used to explain this utility model and do not constitute an improper limitation of this utility model.

[0018] Figure 1 This is a top view of the adhesive biological sample collection tube according to one embodiment of the present invention with the tube cap open.

[0019] Figure 2 for Figure 1 A cross-sectional view of the adhesive biological sample collection tube along the AA plane.

[0020] Figure 3 for Figure 2 The diagram shows a cross-sectional view of the adhesive biological sample collection tube without an adsorption layer.

[0021] In the picture:

[0022] 1. Tube body 2. Tube cap 3. Adsorption layer 4. Scale

[0023] 5. Accommodation space; 6. Annular rib; 7. Wedge block; 8. Limiting block

[0024] 9. Limiting groove; 10. Flexible connector Detailed Implementation

[0025] The present invention will now be described in detail with reference to the accompanying drawings. This description is merely illustrative and explanatory, and should not be construed as limiting the scope of protection of the present invention. Furthermore, those skilled in the art can combine the features in the embodiments described herein and in different embodiments according to the description in this document.

[0026] The embodiments of this utility model are as follows, please refer to... Figures 1-3 An adhesive biological sample collection tube includes a tube body 1 and a tube cap 2. One end of the tube body 1 is closed and the other end is open. The tube cap 2 covers the open end of the tube body 1. An adsorption layer 3 for adhering biological samples is provided on the inner side of the tube cap 2. A scale 4 for marking the position of the biological samples is provided on the tube cap 2. The position of the scale 4 matches the position of the adsorption layer 3. When the tube cap 2 is closed with the tube body 1, the adsorption layer 3 is located inside the tube body 1.

[0027] It should be understood that the adsorption layer 3 has a certain degree of transparency, allowing the scale 4 to be seen through the adsorption layer 3 indicating the position of the biological sample. In specific applications, the required micro-tissue adheres to the surface of the adsorption layer 3 under a microscope. By setting a precision scale 4 at the position of the adsorption layer 3, the acquired sample can be accurately positioned under a microscope, and the target tissue can be visualized for secondary collection. The collection tube design of this utility model eliminates the need to remove unused samples beforehand, allowing the target experimental plan to be implemented directly in this collection tube, greatly improving cell collection efficiency and reducing cell sample loss rate by 50% compared with other existing cell and tissue collection methods.

[0028] To better record the position, scale 4 is coded. Scale 4 and the code are laser-etched in the center of tube cap 2, with the narrowest line thickness reaching 10-15 nm. The collection tube 1 can have volumes of 200 μL, 500 μL, 1.5 mL, and 2 mL.

[0029] Based on the above embodiments, in another embodiment of the present invention, such as Figure 2 and Figure 3 The inner wall of the tube cap 2 is provided with a receiving space 5, and the adsorption layer 3 is filled in the receiving space 5.

[0030] Based on the above embodiments, in another embodiment of the present invention, such as Figure 2 and Figure 3 The inner wall of the tube cap 2 is provided with an annular rib 6, which forms the accommodating space 5.

[0031] Based on the above embodiments, in another embodiment of the present invention, such as Figure 1 The center of the scale 4 coincides with the center of the accommodating space 5.

[0032] Based on the above embodiments, in another embodiment of the present invention, such as Figure 1 The scale 4 is disposed on the inner wall of the tube cap 2; or the tube cap 2 is made of a material with predetermined transparency, and the scale 4 is disposed on the outer wall of the tube cap 2.

[0033] Based on the above embodiments, in another embodiment of the present invention, the adsorption layer 3 is a photocurable resin, an organosilicon resin, or a silicone.

[0034] In one specific embodiment, the adsorption layer 3 is made of silicone resin, and a low-viscosity two-component resin is poured into the accommodating space 5. After potting, a PET or PO film is attached to the adsorption layer 3. In this specific embodiment, the film thickness is 1.5 micrometers, the adsorption layer 3 has a Shore hardness of 5, a coefficient of thermal expansion (PPM / ) of 220, a testing standard of GB / T16920, a thermal conductivity (W / MK) of 0.23, a testing standard of GB / T5598, an effective temperature range (°C) of -60 to 220, a testing standard of GB / T11021, and a withstand voltage of KV / mm. 2 20. Testing standard GB / T1695.

[0035] Based on the above embodiments, in another embodiment of the present invention, such as Figures 1-3 One side of the open end of the tube body 1 is connected to the tube cap 2 via a flexible connector 10, and the other side is fastened to the tube cap 2. Preferably, the tube body 1, tube cap 2, and flexible connector 10 are made of plastic and are integrally injection molded.

[0036] Based on the above embodiments, in another embodiment of the present invention, such as Figure 2 and Figure 3 The tube body 1 has a wedge 7 protruding from its outer surface at its open end. The inner wall of the tube cap 2 has a limiting block 8 with a limiting groove 9. When the tube body 1 and tube cap 2 are fastened together, the limiting block 8 engages with the wedge 7, and the wedge 7 is embedded in the limiting groove 9. With this configuration, when the tube cap 2 is placed on the tube body 1, the lower wall of the tube cap 2 fits snugly against the upper surface of the tube body 1. The limiting block 8, engaged with the wedge 7, provides tension, ensuring a tight fit between the lower wall of the tube cap 2 and the upper surface of the tube body 1. This design not only provides a simple structure and good sealing performance but also prevents the tube cap 2 from falling off, thus preventing sample loss and improving sample safety.

[0037] The same sample was tested using the following method:

[0038] The samples were collected using the gravity method. Because the samples could not be stained, the collection effect could not be visually assessed under a microscope. Since the experiment required a small number of target cells to be collected, the gravity method was prone to loss and the collection efficiency was not high. After collecting 8 samples by cutting, 4 samples were actually tested, with Ct values ​​of 20-31.

[0039] The test used the elastic method to collect samples. However, because the samples could not be stained, the collection effect could not be visually assessed under a microscope. The experiment required a small number of target cells to be collected, and the elastic method was also prone to loss, resulting in low collection efficiency. After collecting 8 samples by cutting, 2 samples were actually detected, with Ct values ​​of 20-30.

[0040] In a comparative experiment with other existing cell and tissue collection methods, eight samples collected using the collection tube of this invention were lysed at 80°C in a 0.2 ml PCR tube. After single-cell PCR amplification, the cDNA was diluted 1:50, and the expression level of the internal reference gene GAPDH was detected. The Ct values ​​of all eight samples were between 19 and 25, indicating successful sample collection and amplification.

[0041] Compared with existing technologies, this invention can accurately locate the acquired samples under a microscope and visualize the secondary collection of target tissues. It can directly collect the target experimental plan in the collection tube without removing unused samples in advance, which greatly improves the cell collection efficiency and reduces the cell sample loss rate by 50% compared with other existing cell and tissue collection methods.

[0042] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. An adhesive biological sample collection tube, comprising a tube body and a cap, wherein one end of the tube body is closed and the other end is open, and the cap covers the open end of the tube body, characterized in that, The inner side of the tube cap is provided with an adsorption layer for adhering biological samples. The tube cap is provided with a scale for marking the position of the biological samples. The position of the scale matches the position of the adsorption layer. When the tube cap is closed with the tube body, the adsorption layer is located inside the tube body.

2. The adhesive biological sample collection tube according to claim 1, characterized in that, The inner wall of the tube cap is provided with a receiving space, and the adsorption layer fills the receiving space.

3. The adhesive biological sample collection tube according to claim 2, characterized in that, The inner wall of the tube cap is provided with annular ribs, which form the accommodating space.

4. The adhesive biological sample collection tube according to claim 2 or 3, characterized in that, The center of the scale coincides with the center of the accommodating space.

5. The adhesive biological sample collection tube according to claim 4, characterized in that, The scale is disposed on the inner wall of the tube cap; or the tube cap is made of a material with predetermined transparency, and the scale is disposed on the outer wall of the tube cap.

6. The adhesive biological sample collection tube according to claim 2 or 3, characterized in that, The adsorption layer is a photocurable resin, organosilicon resin, or silica gel.

7. The adhesive biological sample collection tube according to any one of claims 1 to 3, characterized in that, One side of the open end of the tube is connected to the tube cap via a flexible connector, and the other side is fastened to the tube cap.

8. The adhesive biological sample collection tube according to any one of claims 1 to 3, characterized in that, The tube body has a wedge protruding from the outer surface of the tube body at the open end. The inner wall of the tube cap has a limiting block with a limiting groove. When the tube body and the tube cap are fastened together, the limiting block is fastened to the wedge and the wedge is embedded in the limiting groove.

Images