Cell cryopreservation bag

Abstract

The utility model provides a cell cryopreservation bag which comprises a bag body, an infusion assembly and a reserved sample vesicle, and the infusion assembly and the reserved sample vesicle are both communicated with the bag body. The infusion assembly comprises a main pipe, a liquid adding pipe and a liquid outlet pipe, the liquid adding pipe and the liquid outlet pipe are both connected with the main pipe, and the sample reserving vesicle is used for reserving a liquid sample in the bag body. According to the utility model, the liquid in the bag body can be conveniently detected.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical cryopreservation bag technical field especially relates to a cell cryopreservation bag. BACKGROUND

[0002] Human umbilical cord mesenchymal stem cells are a kind of cells obtained by sterile collection, purification and expansion through tissue separation and cell culture technology after the delivery of fetus, which are originally cut off and discarded umbilical cord segments. Human umbilical cord mesenchymal stem cells can repair or replace damaged tissues and organs, and can also treat degenerative diseases of bones and muscles, and can regulate the body's immunity, which is expected to be a new alternative therapy for stem cell therapy, bringing new hope to the majority of critically ill patients. Stem cell therapy is usually preserved by freezing method, and in the prior art, the cell cryopreservation bag may be contaminated by microorganisms before storage, and the operation of injecting the cell cryopreservation suspension into the cell cryopreservation bag, and the cryopreservation bag and the syringe consumables themselves may be contaminated by microorganisms, and the bubbles existing in the bag may also cause damage to the cells during freezing and thawing.

[0003] The utility model with publication number CN213229719U discloses a cell therapy drug cryopreservation bag, which comprises a main bag body and two supports, the main bag body has a first end and a second end arranged opposite in the up-down direction, the first end is provided with a first input pipe and an output pipe, two mounting parts are arranged on the main bag body and arranged opposite in the circumferential direction of the main bag body, and each support is connected with a mounting part to support the main bag body. However, the above-mentioned cell therapy drug cryopreservation bag needs to use the only output pipe for sterile detection of the drug in the bag body after injection, which makes sample inspection difficult and may contaminate the drug in the bag body during the inspection process. UTILITY MODEL CONTENT

[0004] The utility model aims at solving the defects in the prior art and provides a cell cryopreservation bag for detecting the liquid in the bag body.

[0005] To achieve the above-mentioned purpose, the utility model provides a cell cryopreservation bag, which comprises a bag body, an infusion assembly and a sample retaining vesicle, the infusion assembly and the sample retaining vesicle are communicated with the bag body.

[0006] The infusion assembly comprises a main pipe, a liquid adding pipe and a liquid outlet pipe, the liquid adding pipe and the liquid outlet pipe are connected with the main pipe, and the sample retaining vesicle is used for retaining the liquid sample in the bag body.

[0007] A sample holding bubble is arranged at the top end of the bag body, the liquid outlet pipe is closed, the liquid is injected into the bag body through the liquid adding pipe and the main pipe, the liquid adding pipe and the main pipe are closed, after the liquid in the bag body is mixed, a small amount of liquid enters the sample holding bubble, then the conduit connecting the sample holding bubble and the bag body is heat sealed, and the sample holding bubble is removed to complete the sample holding, so as to check whether the consumables such as the infusion bag, the syringe and the like are polluted after the liquid is injected.

[0008] Optionally, the sample holding bubble is a negative pressure bubble and is arranged above the bag body.

[0009] Since the sample holding bubble is arranged above the bag body, through the density principle and the negative pressure, the small bubbles difficult to be discharged from the bag body can be sucked into the sample holding bubble during the sample holding, the excess bubbles in the bag body are discharged, and the damage to the cells in the bag body is reduced.

[0010] Optionally, the sample holding bubble is communicated with the bag body through a sample holding pipe, and a flow control device is arranged on the sample holding pipe.

[0011] The flow control device can control the volume of the sample suspension in the sample holding bubble by adjusting the flow rate.

[0012] Optionally, the flow control device is one of a screw thread bayonet, a liquid stopper, a conduit regulator and a flow stopper.

[0013] Optionally, the liquid adding pipe is provided with a liquid adding valve for opening or closing the liquid adding pipe.

[0014] Optionally, the liquid outlet pipe is provided with a liquid outlet valve for opening or closing the liquid outlet pipe.

[0015] Optionally, the bag body is made of a flexible material.

[0016] Beneficial effects:

[0017] The utility model discloses a bag body, infusion assembly and sample holding bubble, infusion assembly and sample holding bubble all are communicated with bag body, sample holding bubble is used for keeping the liquid sample in bag body. A sample holding bubble is arranged on the bag body, the liquid outlet pipe is closed, the liquid is injected into the bag body through the liquid adding pipe and the main pipe, the liquid adding pipe and the main pipe are closed, after the liquid in the bag body is mixed, a small amount of suspension enters the sample holding bubble, then the conduit connecting the sample holding bubble and the bag body is heat sealed, and the sample holding bubble is removed to complete the sample holding, so as to check whether the consumables such as the infusion bag, the syringe and the like are polluted after the liquid is injected. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the description of the embodiments or the prior art. Obviously, the drawings described below only represent some of the embodiments of the present application, and for those skilled in the art, other drawings can also be obtained according to the structures shown in the drawings without any creative effort.

[0019] Figure 1 A structure diagram of a cell cryopreservation bag is disclosed in the present application.

[0020] The drawings show that: 1 bag body; 2 infusion assembly; 21 main pipe; 22 liquid filling pipe; 23 liquid outlet pipe; 3 sample retaining bubble; 31 sample retaining pipe; 4 flow controller.

[0021] The implementation, functional features and advantages of the present application will be further described with reference to the embodiments and the accompanying drawings. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments only represent some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without any creative effort fall within the scope of protection of the present application.

[0023] In the description of the present application, unless otherwise explicitly defined, the words such as setting, installing, connecting, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in the present application in combination with the specific content of the technical solutions.

[0024] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "exemplary embodiment", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in combination with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the exemplary description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the described specific features, structures, materials or characteristics can be combined in any one or more embodiments or examples in a suitable manner.

[0025] Referring to Figure 1 A cell cryopreservation bag according to the embodiments of the present application comprises a bag body 1, an infusion assembly 2 and a sample retaining bubble 3, wherein the infusion assembly 2 and the sample retaining bubble 3 are both in communication with the bag body 1.

[0026] The infusion assembly 2 comprises a main pipe 21, a liquid adding pipe 22 and a liquid outlet pipe 23, the liquid adding pipe 22 and the liquid outlet pipe 23 are connected with the main pipe 21, and the sample holding bubble 3 is used for holding the liquid sample in the bag body 1.

[0027] Specifically, the liquid in the bag body 1 is a cell cryopreservation suspension, a sample holding bubble 3 is arranged at the top end of the bag body 1, the liquid outlet pipe 23 is closed, the cell cryopreservation suspension is injected into the bag body 1 through the liquid adding pipe 22 and the main pipe 21, the liquid adding pipe 22 and the main pipe 21 are closed, after the cell cryopreservation suspension in the bag body 1 is mixed, 1-3 ml of the suspension enters the sample holding bubble 3, the sample holding bubble 3 and the pipe connected with the bag body 1 are heat sealed, and the sample holding bubble 3 is removed, so that the sample holding is completed, and whether the infusion operation or the cryopreservation bag, the syringe and other consumables are polluted after the cell cryopreservation suspension is injected can be checked.

[0028] In some embodiments of the utility model, the sample holding bubble 3 is a negative pressure bubble, and it is arranged above the bag body 1.

[0029] Because the sample holding bubble 3 is arranged above the bag body 1, through the density principle and negative pressure, the small bubbles difficult to exhaust in the bag body 1 can be sucked into the sample holding bubble 3 when the sample holding is carried out, the remaining bubbles in the bag are beneficial to exhaust, and the damage to the cells in the bag body 1 is reduced.

[0030] In some embodiments of the utility model, the sample holding bubble 3 is communicated with the bag body 1 through a sample holding pipe 31, and a flow control device 4 for controlling the flow rate is arranged on the sample holding pipe 31.

[0031] The flow control device 4 can control the volume of the sample holding suspension in the sample holding bubble 3 by adjusting the flow rate.

[0032] In some embodiments of the utility model, the flow control device 4 is one of a screw thread bayonet, a liquid stopper, a pipe regulator and a flow breaker.

[0033] In some embodiments of the utility model, a liquid adding valve for opening or closing the liquid adding pipe 22 is arranged on the liquid adding pipe 22.

[0034] In some embodiments of the utility model, a liquid outlet valve for opening or closing the liquid outlet pipe 23 is arranged on the liquid outlet pipe 23.

[0035] In some embodiments of the utility model, the bag body 1 is made of a flexible material.

[0036] The above are only preferred embodiments of the utility model, and do not limit the patent range of the utility model, and any equivalent structural transformation, direct / indirect application in other related technical fields under the utility model concept of the utility model, and the contents of the utility model specification and drawings are included in the patent protection range of the utility model.

Claims

1. A cell cryopreservation bag, characterized by, The bag (1), infusion assembly (2) and sample retention bubble (3) are communicated with the bag (1); The infusion assembly (2) comprises a main pipe (21), a liquid adding pipe (22) and a liquid outlet pipe (23), the liquid adding pipe (22) and the liquid outlet pipe (23) are connected with the main pipe (21), and the sample retention bubble (3) is used for retaining the liquid sample in the bag (1).

2. The cell cryobag of claim 1, wherein, The sample retention bubble (3) is a negative pressure bubble and is arranged above the bag (1).

3. The cell cryobag of claim 1, wherein, The sample retention bubble (3) is communicated with the bag (1) through a sample retention pipe (31), and a flow control device (4) for controlling the flow rate is arranged on the sample retention pipe (31).

4. The cell cryobag of claim 3, wherein, The flow control device (4) is one of a screw thread socket, a liquid stopper, a catheter regulator and a flow breaker.

5. The cell cryobag according to any one of claims 1 to 4, characterized in that, A liquid adding valve for opening or closing the liquid adding pipe (22) is arranged on the liquid adding pipe (22).

6. The cell cryobag according to any one of claims 1 to 4, characterized in that, A liquid outlet valve for opening or closing the liquid outlet pipe (23) is arranged on the liquid outlet pipe (23).

7. The cell cryobag according to any one of claims 1 to 4, wherein, The bag (1) is made of a flexible material.

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