Simple device for enriching substances to be detected

Abstract

The utility model discloses a simple device for enriching an object to be detected, and relates to the technical field of electrochemical detection. The simple to-be-detected object enrichment device comprises an enrichment area, a sample inlet, a sample outlet and a sample outlet, and the sample outlet is located at the bottom of the enrichment area; the adsorption electrode pair is detachably arranged on the outer side wall of the enrichment area; the push-pull rod is arranged at the bottom of the enrichment area and can axially slide, and the sample outlet is controlled to be opened or closed by sliding the push-pull rod. According to the simple device for enriching the object to be detected, the problems of complicated operation and high equipment dependence degree in the prior art are solved through combination of electrode adsorption and manual fluid control. Compared with a traditional device, on the premise that the enrichment efficiency is guaranteed, the operation complexity and the manufacturing cost are remarkably reduced.

Description

Technical Field

[0001] This utility model relates to the field of electrochemical detection technology, and in particular to a simple device for enriching analytes. Background Technology

[0002] Electrode enrichment technology is a pre-concentration method based on electrochemical principles. It achieves concentration of trace substances (typically in the concentration range of 10⁻⁶) by controlling the interfacial forces (including electrostatic adsorption, coordination bonding, and hydrophobic interactions) between the electrode surface and the target substance in the solution. -9 -10 -12 The technology enables the directional aggregation of analytes (mol / L) on the electrode surface. By increasing the local concentration of the target analyte by 2-3 orders of magnitude, this technique effectively overcomes the sensitivity bottleneck of traditional detection methods and has been successfully applied in cutting-edge fields such as environmental pollutant monitoring (e.g., heavy metal ion detection), biomarker screening (e.g., circulating tumor DNA capture), and isolation of foodborne pathogens.

[0003] Although existing electrode enrichment devices have been commercialized, they still have significant technical shortcomings: ① The operation process relies on manual intervention, especially in the sample loading, buffer replacement and electrode regeneration stages, which lack automated modules, resulting in long processing times per cycle; ② The system integration is insufficient, requiring high skill levels from operators; ③ Maintenance costs are high, and the modified layer on the electrode surface is prone to peeling failure during repeated use, significantly increasing the cost of use. Utility Model Content

[0004] The technical problem to be solved by this utility model is at least one of the problems mentioned in the background art, and provides a simple device for enriching the analyte.

[0005] To solve the above problems, the present invention proposes the following technical solution:

[0006] A simple device for enriching an analyte, comprising:

[0007] An enrichment zone having an inlet and an outlet, the outlet being located at the bottom of the enrichment zone;

[0008] An adsorption electrode pair, wherein the adsorption electrode pair is detachably disposed on the outer wall of the enrichment region;

[0009] A push-pull rod is located at the bottom of the enrichment area and can slide axially. The opening or closing of the sample outlet is controlled by sliding the push-pull rod.

[0010] A further technical solution includes a waste liquid tank, which is connected to the enrichment zone when the sample outlet is opened.

[0011] A further technical solution includes a shell, wherein the enrichment zone and the waste liquid tank are disposed within the shell.

[0012] A further technical solution is that the housing is provided with a groove, the push-pull rod is embedded in the groove, and the push-pull rod can slide within the groove.

[0013] A further technical solution is that the shell is provided with a liquid outlet channel, one end of which is connected to the waste liquid chamber; when the sample outlet is opened, the other end of the liquid outlet channel is connected to the enrichment zone.

[0014] A further technical solution is that the liquid outlet channel is located between the push-pull rod and the waste liquid tank.

[0015] A further technical solution is that the material of the shell is at least one of glass, plastic or resin.

[0016] A further technical solution is that the enrichment region is an inverted conical funnel structure.

[0017] A further technical solution is that the volume of the enrichment region is greater than or equal to 10 mL.

[0018] A further technical solution is that the electrode of the adsorption electrode pair is one of a carbon-based electrode, a metal oxide electrode, or a polymer electrode.

[0019] Compared with the prior art, the technical effects achieved by this utility model include:

[0020] This invention provides a simplified analyte enrichment device comprising: an enrichment zone having an inlet and an outlet, the outlet being located at the bottom of the enrichment zone; an adsorption electrode pair detachably mounted on the outer wall of the enrichment zone; and a push-pull rod located at the bottom of the enrichment zone and capable of axial sliding, controlling the opening or closing of the outlet by sliding the push-pull rod. This simplified analyte enrichment device solves the problems of complex operation and high equipment dependence in existing technologies by combining electrode adsorption with manual fluid control. Compared to traditional devices, this invention significantly reduces operational complexity and manufacturing costs while maintaining enrichment efficiency. Attached Figure Description

[0021] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 A schematic diagram of the simplified enrichment device for the test substance provided by this utility model.

[0023] Figure Labels

[0024] Electrode 1, enrichment zone 2, push-pull rod 3, waste liquid tank 4, shell 5, sample outlet 6. Detailed Implementation

[0025] The technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Similar component reference numerals in the drawings represent similar components. Obviously, the embodiments described below are only some embodiments of this utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0026] It should be understood that, when used in this specification and the appended claims, the terms "comprising" and "including" indicate the presence of the described features, integrals, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components and / or collections thereof.

[0027] It should also be understood that the terminology used in this specification of embodiments of the present invention is for the purpose of describing particular embodiments only and is not intended to limit the embodiments of the present invention. As used in this specification of embodiments of the present invention and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0028] Example 1

[0029] See Figure 1 This utility model provides a simple device for enriching a analyte. As shown in the figure, the simple device for enriching a analyte includes:

[0030] Enrichment zone 2, the enrichment zone 2 having an inlet and an outlet 6, the outlet 6 being located at the bottom of the enrichment zone 2;

[0031] An adsorption electrode pair, wherein the adsorption electrode pair comprises at least one pair of electrodes 1, and the electrodes 1 are detachably disposed on the outer wall of the enrichment region 2;

[0032] Push-pull rod 3 is located at the bottom of enrichment zone 2 and can slide axially. The opening or closing of the sample outlet 6 is controlled by sliding the push-pull rod 3.

[0033] In this embodiment, the simplified enrichment device for the analyte also includes a waste liquid chamber 4. When the sample outlet 6 is opened, the waste liquid chamber 4 is connected to the enrichment zone 2.

[0034] In this embodiment, the simplified device for enriching the analyte also includes a housing 5, and the enrichment zone 2 and the waste liquid tank 4 are disposed in the housing 5.

[0035] In practice, the waste liquid tank 4 is detachably connected to the shell 5 and is equipped with an anti-backflow vent with a diameter of 0.5mm.

[0036] In a specific implementation, the housing 5 is provided with a groove, the push-pull rod 3 is embedded in the groove, and the push-pull rod 3 can slide within the groove.

[0037] The push-pull rod 3 uses a sealing head made of polytetrafluoroethylene (PTFE) and is embedded in the groove. By sliding axially within the groove, it controls the connection and disconnection between the enrichment zone 2 and the waste liquid tank 4. In some embodiments, the sliding stroke is 15–25 mm.

[0038] In a specific implementation, the housing 5 is provided with a liquid outlet channel, one end of which is connected to the waste liquid chamber; when the sample outlet 6 is opened, the other end of the liquid outlet channel is connected to the enrichment zone 2. In a further embodiment, the liquid outlet channel is located between the push-pull rod 3 and the waste liquid chamber 4. This embodiment achieves precise entry of the liquid enriched in the enrichment zone 2 into the waste liquid chamber 4 by providing a liquid outlet channel in the housing 5 to guide the liquid.

[0039] In specific implementation, the shell 5 is made of at least one of glass, plastic, or resin. It should be noted that the shell 5 is made of an insulating material to ensure the safety of the device, such as PVC, PE, or polystyrene. The overall configuration of the shell 5 supports standardized packaging such as cuboids and cylinders, adapting to typical laboratory operating scenarios.

[0040] In some embodiments, the enrichment zone 2 is an inverted conical funnel structure with a cone angle of 60°±5° and a sample outlet 6 diameter of 2–5 mm. For example, in this embodiment, the sample outlet 6 diameter is 3 mm. The opening of the conical funnel structure is the sample inlet.

[0041] In some embodiments, the volume of the enrichment zone 2 is greater than or equal to 10 mL. For example, in this embodiment, the volume of the enrichment zone 2 is 12 mL. In other embodiments, the volume of the enrichment zone 2 is 15 mL. Understandably, the capacity of the waste liquid tank 4 is adapted to the maximum processing capacity of the enrichment zone.

[0042] In some embodiments, electrode 1 of the adsorption electrode pair is one of a carbon-based electrode (porous carbon / carbon nanotubes), a metal oxide electrode (such as indium tin oxide), or a polymer electrode (polyaniline / polypyrrole). The bio-microspheres (particle size 100-500 nm) in the sample are captured by electrostatic adsorption generated by the adsorption electrode pair.

[0043] The simplified enrichment device for analytes provided by this invention utilizes the enrichment zone 2 of the inverted funnel structure. A tapered structure guides the liquid flow to the sample outlet at the bottom of the enrichment zone 2. Adsorption electrodes on the outer wall of the enrichment zone 2 adsorb the target analyte (e.g., biospheres) in the sample under energized conditions. When the push-pull rod 3 is in its initial position, the sample outlet 6 is closed to maintain a stable liquid level in the enrichment zone 2, thus achieving target analyte adsorption. Pulling the push-pull rod 3 opens the sample outlet 6, and the waste liquid flows into the waste liquid chamber 4 by gravity. This invention solves the problems of complex operation and high equipment dependence in existing technologies by combining electrode adsorption with manual fluid control. Compared to traditional devices, this invention significantly reduces operational complexity and manufacturing costs while ensuring enrichment efficiency.

[0044] The operation procedure of the simplified enrichment device for the analyte provided by this utility model is as follows:

[0045] (1) Inject the sample to be tested into the enrichment area and turn on the electrode power supply to make it adsorb.

[0046] (2) After the target object is adsorbed onto the electrode surface, manually push the push-pull rod to open the bottom channel;

[0047] (3) The waste liquid flows into the waste liquid tank through the bottom of the funnel, and then the push-pull rod is closed to block the channel;

[0048] (4) Inject cleaning solution into the enrichment zone and repeat the adsorption-emission process to remove impurities from the surface of the target object;

[0049] (5) The target material that is finally retained on the electrode surface is enriched and can be directly used for subsequent detection.

[0050] The above operations can easily achieve the enrichment and cleaning of analytes in samples.

[0051] In the above embodiments, the descriptions of each embodiment have different focuses. For parts that are not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0052] The above description describes specific embodiments of this utility model, but the scope of protection of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this utility model, and these modifications or substitutions should all be covered within the scope of protection of this utility model. Therefore, the scope of protection of this utility model should be determined by the scope of the claims.

Claims

1. A simple device for enriching an analyte, characterized in that, include: An enrichment zone having an inlet and an outlet, the outlet being located at the bottom of the enrichment zone; An adsorption electrode pair, wherein the adsorption electrode pair is detachably disposed on the outer wall of the enrichment region; A push-pull rod is located at the bottom of the enrichment area and can slide axially. The opening or closing of the sample outlet is controlled by sliding the push-pull rod.

2. The simplified apparatus for enriching the analyte as described in claim 1, characterized in that, It also includes a waste liquid tank, which is connected to the enrichment zone when the sample outlet is opened.

3. The simplified apparatus for enriching the analyte as described in claim 2, characterized in that, It also includes a housing, in which the enrichment zone and waste liquid tank are disposed.

4. The simplified apparatus for enriching the analyte as described in claim 3, characterized in that, The housing has a groove, the push-pull rod is embedded in the groove, and the push-pull rod can slide within the groove.

5. The simplified apparatus for enriching the analyte as described in claim 3, characterized in that, The shell is provided with a liquid outlet channel, one end of which is connected to the waste liquid chamber; when the sample outlet is opened, the other end of the liquid outlet channel is connected to the enrichment zone.

6. The simplified apparatus for enriching the analyte as described in claim 5, characterized in that, The liquid outlet channel is located between the push-pull rod and the waste liquid tank.

7. The simplified apparatus for enriching the analyte as described in claim 3, characterized in that, The shell is made of at least one of glass, plastic or resin.

8. The simplified apparatus for enriching the analyte as described in claim 1, characterized in that, The enrichment region has an inverted conical funnel structure.

9. The simplified apparatus for enriching the analyte as described in claim 1, characterized in that, The volume of the enrichment zone is greater than or equal to 10 mL.

10. The simplified apparatus for enriching the analyte as described in claim 1, characterized in that, The electrodes of the adsorption electrode pair are one of carbon-based electrodes, metal oxide electrodes, and polymer electrodes.

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