High-flux reaction tube

Abstract

The utility model provides a high flux reaction tube, the reaction tube is applied to mixing equipment to complete uniform mixing of reagents in the reaction tube, the reaction tube comprises a tube body, a grabbing ring groove and at least one buckle ring groove, when the reagents are discharged from the lower end of the tube body, the inner cavity of the tube body is divided into a solution reaction chamber and a valve core containing chamber through a funnel-shaped spacer block; a valve element mechanism is arranged in the valve element containing chamber and used for discharging reagents out of the solution reaction chamber from the lower end of the pipe body. The jacking valve core is arranged at the bottom of the reaction tube to drive the closed ring to be opened, so that liquid in the solution reaction region flows out from the lower part, and the reaction tube is filled with reaction liquid from the top and flows out from the bottom after the reaction is finished, and the working procedures are saved.

Description

TECHNICAL FIELD

[0001] The utility model relates to reaction tube technical field especially relates to high throughput reaction tube. BACKGROUND

[0002] At present, solid-liquid phase biochemical reaction in the common test tube such as 15 mL, 50 mL centrifugal tube is usually in the additional mixing device such as shaking bed, mixing instrument, oscillator to make each component in reaction system continuously fully mixed, generally needs to take down the reaction tube from the equipment after mixing, needs to repeat a process, how not to take down the reaction tube process can take down the reagent after mixing from the equipment, and it is urgent to develop and research. UTILITY MODEL CONTENT

[0003] To solve the above technical problem, the utility model adopts the technical scheme of high throughput reaction tube, the reaction tube is applied to mixing equipment, and mixing of reagent in the reaction tube is completed, and the reaction tube comprises:

[0004] A tube body 1 is penetrated through up and down;

[0005] A grabbing ring groove 2 is used for test tube grabbing or placing;

[0006] At least one buckle ring groove 3 is used for fixing reaction test tube in mixing equipment;

[0007] Wherein, the grabbing ring groove 2 and the buckle ring groove 3 are arranged at the upper end of the tube body 1, and the grabbing ring groove 2 is located above the buckle ring groove 3;

[0008] When the tube body 1 discharges reagent from the lower end, the inner cavity of the tube body 1 is divided into a solution reaction chamber 101 and a valve core assembly chamber 102 through a funnel-shaped partition block 103;

[0009] A valve core mechanism 4 is arranged in the valve core assembly chamber 102, and is used for discharging reagent from the solution reaction chamber 101 outside the tube body 1.

[0010] Further, the valve core mechanism 4 comprises:

[0011] A closed ring 401 is located in the solution reaction chamber 101 at one end, and is located in the valve core assembly chamber 102 at the other end;

[0012] A valve core 402 is inserted into the lower end of the closed ring 401 at one end, and is located in the valve core assembly chamber 102 at the other end, and the end face cylindrical head part exists gap with the inner wall of the lower end of the tube body 1;

[0013] A spring 403 is used for providing spring force for the valve core, is sleeved on the valve core 402, and is located between the gap of the valve core 402 and the tube body 1.

[0014] Further, the closed ring 401 is tightly attached to the inner wall of the funnel-shaped partition block 103 under the action of spring force, for sealing the solution reaction chamber 101 from liquid leakage;

[0015] When liquid needs to be discharged, the valve core is lifted by the device outside the bottom of the reaction tube, driving the closed ring to open, so that the liquid in the solution reaction chamber 101 flows out from the bottom, and the reaction tube is filled with reaction liquid from the top. After the reaction is completed, the operation process is discharged from the bottom.

[0016] Further, the top of the closed ring 401 is spherical, and when the closed ring is opened to discharge liquid, the solution reaction area of the tube body and the closed ring avoid storing liquid, which is easy to discharge;

[0017] The spherical top of the closed ring is treated with a hydrophobic coating on the surface, which is beneficial to water drainage;

[0018] The closed ring 401 is made of an elastic material that is compatible with biological reagents, preferably silicone.

[0019] Further, the middle segment of the closed ring 401 is a conical body that is attached to the inner wall of the funnel-shaped partition block 103;

[0020] Under the action of spring force, the edge of the conical body of the middle segment of the closed ring 401 is deformed to tightly attach to the conical inner wall of the funnel-shaped partition block 103, achieving liquid sealing;

[0021] The taper angle of the conical body of the middle segment of the closed ring 401 is greater than the taper angle of the funnel-shaped partition block 103, so that the reaction reagent is sealed at the upper end of the closed ring 401.

[0022] Further, the lower segment of the closed ring 401 is a straight cylinder, and the outer diameter of the straight cylinder is smaller than the size of the small diameter straight hole of the tube body, having a liquid discharge gap;

[0023] The closed ring 401 is installed by cooperating with the stepped column at the top end of the valve core 402 through the annular stepped groove, and during installation, the stepped column at the top end of the valve core is installed into the annular stepped groove of the closed ring through the elastic deformation of the closed ring 401.

[0024] After installation, the pull-out force between the closed ring 401 and the valve core 402 is greater than the spring.

[0025] Further, the diameter of the stepped cylinder at the bottom of the valve core 402 is smaller than the diameter of the valve core assembly chamber of the tube body, so that the liquid flows out from the peripheral gap;

[0026] The outer end surface of the stepped cylinder at the bottom of the valve core 402 is provided with a central tapered hole, which is aligned with the central position of the valve core 402 when the lifting device outside the bottom of the reaction tube lifts the valve core.

[0027] The bottom step outer cylinder surface of the valve core 402 is circumferentially provided with a plurality of through grooves for draining water.

[0028] The top step column upper edge of the valve core is provided with a rounded corner, which facilitates installation during assembly with the sealing ring 401.

[0029] The valve core 402 is preferably made of a material that is compatible with the reaction solution, preferably polypropylene material, preferably Teflon material.

[0030] Further, the spring 403 provides a sealing compression force for the sealing ring 401, preferably a helical linear spring; the spring 403 is preferably made of stainless steel.

[0031] Further, when the pipe body 1 is used to discharge reagent from the upper end, the lower end of the pipe body 1 is a closed flat bottom, and the flat bottom is suitable for a mixing device with a heating magnet.

[0032] Further, the inner wall of the pipe body 1 is also provided with a protrusion for increasing the rotation force of the liquid when rotating in the mixing device.

[0033] Further, a plurality of the reaction tubes are arranged on a 96-well plate to form a high-throughput reaction well plate.

[0034] The utility model has the advantages and positive effects that when it is necessary to drain liquid from the bottom, the valve core mechanism is arranged in the pipe body, the bottom device of the reaction tube lifts the valve core, drives the sealing ring to open, makes the liquid in the solution reaction chamber 101 flow out from the lower part, and the reaction tube is filled with reaction liquid from the top, and after the reaction is completed, the operation process of flowing out from the bottom;

[0035] The grabbing ring groove is convenient for grabbing or placing the test tube.

[0036] The buckle ring groove is convenient for fixing the reaction test tube in the mixing device. DETAILED DESCRIPTION

[0037] Figure 1 The structure diagram of the high-throughput reaction tube described in Example 1;

[0038] Figure 2 The perspective view of the high-throughput reaction tube described in Example 1;

[0039] Figure 3 The structure diagram of the high-throughput reaction tube described in Example 2;

[0040] In the figure: 1, pipe body; 101, solution reaction chamber; 102, valve core assembly chamber; 103, funnel-shaped partition; 2, grabbing ring groove; 3, buckle ring groove; 4, valve core mechanism; 401, sealing ring; 402, valve core; 403, spring. DETAILED DESCRIPTION

[0041] In order to better understand the utility model, the utility model is further described below in combination with specific embodiments and drawings.

[0042] As Figures 1-2 shown, the high-throughput reaction tube is applied to a mixing device to complete mixing of reagents in the reaction tube, and the reaction tube comprises:

[0043] a tube body 1, which is a tube body penetrating through from top to bottom;

[0044] a grabbing ring groove 2 for grabbing or placing the reaction tube;

[0045] at least one buckle ring groove 3 for fixing the reaction tube in the mixing device;

[0046] wherein the grabbing ring groove 2 and the buckle ring groove 3 are arranged at the upper end of the tube body 1, and the grabbing ring groove 2 is located above the buckle ring groove 3;

[0047] when the tube body 1 discharges reagents from the lower end, the inner cavity of the tube body 1 is divided into a solution reaction chamber 101 and a valve core assembly chamber 102 by the funnel-shaped partition block 103;

[0048] the valve core assembly chamber 102 is provided with a valve core mechanism 4 for discharging the reagents from the solution reaction chamber 101 to the outside of the tube body 1 from the lower end of the tube body 1.

[0049] Further, the valve core mechanism 4 comprises:

[0050] a closed ring 401, one end of which is located in the solution reaction chamber 101 and the other end of which is located in the valve core assembly chamber 102;

[0051] a valve core 402, one end of which is inserted into the lower end of the closed ring 401 and the other end of which is located in the valve core assembly chamber 102, and the end face cylindrical head portion of which has a gap with the inner wall of the lower end of the tube body 1;

[0052] a spring 403 for providing spring force for the valve core, which is sleeved on the valve core 402 and located between the valve core 402 and the gap of the tube body 1.

[0053] Further, the closed ring 401 tightly abuts the inner wall of the funnel-shaped partition block 103 under the action of the spring force, so as to seal the solution reaction chamber 101 without liquid leakage;

[0054] Further, the top of the closed ring 401 is spherical, and when the closed ring is opened to discharge liquid, the solution reaction area of the tube body and the closed ring avoid liquid storage and are easy to discharge;

[0055] the spherical top of the closed ring is subjected to surface hydrophobic coating treatment, which is beneficial to water discharge;

[0056] The closed ring 401 is made of elastic material compatible with biological reagents, preferably silica gel.

[0057] Further, the middle section of the closed ring 401 is a conical body that fits the inner wall of the funnel-shaped partition block 103;

[0058] Under the action of spring force, the edge of the conical body of the middle section of the closed ring 401 deforms tightly against the conical inner wall of the funnel-shaped partition block 103, achieving liquid sealing;

[0059] The taper angle of the conical body of the middle section of the closed ring 401 is greater than the taper angle of the funnel-shaped partition block 103, so as to seal the reaction reagent in the upper end of the closed ring 401.

[0060] Further, the lower section of the closed ring 401 is a straight cylinder, and the outer diameter of the straight cylinder is smaller than the size of the small-diameter straight hole of the pipe body, having a liquid discharge gap;

[0061] The closed ring 401 is installed by cooperating with the stepped column at the top end of the valve core 402 through the annular stepped groove, and in the installation process, the stepped column at the top end of the valve core is installed into the annular stepped groove of the closed ring through the elastic deformation of the closed ring 401;

[0062] The pull-out force between the closed ring 401 and the valve core 402 after installation is greater than the spring.

[0063] Further, the diameter of the stepped cylinder at the bottom of the valve core 402 is smaller than the diameter of the valve core assembly chamber of the pipe body, so that the liquid flows out from the peripheral gap;

[0064] The outer end surface of the bottom stepped cylinder of the valve core 402 is provided with a central tapered hole, which is aligned with the central position of the valve core 402 when the external lifting device at the bottom of the reaction tube opens the valve core;

[0065] A plurality of through grooves are circumferentially arranged on the outer cylindrical surface of the bottom stepped cylinder of the valve core 402, which are used for draining water;

[0066] The upper edge of the top stepped column of the valve core is provided with a rounded corner, which facilitates installation and guidance during assembly with the closed ring 401.

[0067] The valve core 402 is preferably made of a material compatible with the reaction liquid, preferably polypropylene material, and preferably Teflon material.

[0068] Further, the spring 403 provides a sealing and pressing force for the closed ring 401, and is preferably a helical linear spring; the spring 403 is preferably made of stainless steel.

[0069] Its working process: when liquid needs to be discharged, the valve core is lifted by the device outside the bottom of the reaction tube, which drives the closed ring to open, so that the liquid in the solution reaction chamber 101 flows out from the lower part, and the reaction tube is filled with reaction liquid from the top, and the operation process of the liquid flowing out from the bottom after the reaction is completed.

[0070] Example 2

[0071] As shown in Figure 3 different from example 1, when the tube body 1 is used for discharging reagent from the upper end, the lower end of the tube body 1 is a closed flat bottom, and the flat bottom is suitable for a mixing device with a heating magnet.

[0072] Further, the inner wall of the tube body 1 is also provided with a convex (or a rotating blade) for increasing the rotation force of the liquid when rotating in the mixing device.

[0073] In addition, a screw thread can be additionally arranged at the top of the reaction tube, and a corresponding functional cap unit can be installed, so as to reserve the function expansion.

[0074] Example 3

[0075] The plurality of reaction tubes described in examples 1 and 2 are arranged on a 96-hole plate to form a high-throughput reaction hole plate.

[0076] The above describes the embodiments of the present application in detail, but the content described above is only the preferred embodiments of the present application, and cannot be considered as limiting the scope of the present application. Any equivalent changes and improvements within the scope of the present application should still belong to the scope of the present patent.

Claims

1. A high throughput reaction tube, characterized by: The reaction tube is applied to a mixing device to complete mixing of reagents in the reaction tube, and the reaction tube comprises: a tube body (1) which is through from top to bottom; a grabbing ring groove (2) for grabbing or placing the reaction tube; at least one buckle ring groove (3) for fixing the reaction tube on the mixing device; wherein the grabbing ring groove (2) and the buckle ring groove (3) are arranged at the upper end of the tube body (1), and the grabbing ring groove (2) is located above the buckle ring groove (3); when the tube body (1) discharges reagents from the lower end, the inner cavity of the tube body (1) is divided into a solution reaction chamber (101) and a valve core assembly chamber (102) by a funnel-shaped partition block (103); the valve core assembly chamber (102) is provided with a valve core mechanism (4) for discharging the reagents from the lower end of the tube body (1) out of the solution reaction chamber (101).

2. The high throughput reaction tube of claim 1, wherein: The valve core mechanism (4) comprises: a closing ring (401) which is located in the solution reaction chamber (101) at one end and in the valve core assembly chamber (102) at the other end; a valve core (402) which is inserted into the lower end of the closing ring (401) at one end and located in the valve core assembly chamber (102) at the other end, and the end face cylindrical head portion of which has a gap with the inner wall of the lower end of the tube body (1); a spring (403) for providing spring force for the valve core, which is sleeved on the valve core (402) and located between the valve core (402) and the gap of the tube body (1).

3. The high throughput reaction tube of claim 2, wherein: The closing ring (401) is tightly attached to the inner wall of the funnel-shaped partition block (103) under the action of the spring force, so as to seal the solution reaction chamber (101) and prevent liquid leakage; when liquid needs to be discharged, the valve core is lifted by a device externally arranged at the bottom of the reaction tube, the closing ring is opened, the liquid in the solution reaction chamber (101) flows out from the lower part, and the reaction tube is filled with reaction liquid from the top, and the operation process is completed after the reaction.

4. The high throughput reaction tube of claim 1, wherein: The top of the closing ring (401) is spherical, and the solution reaction area of the tube body and the closing ring are free of liquid storage and easy to discharge when the closing ring is opened to discharge liquid; the spherical top of the closing ring is subjected to hydrophobic coating treatment, which is beneficial to water discharge; the closing ring (401) is made of an elastic material which is compatible with biological reagents.

5. The high throughput reaction tube of claim 4, wherein: The middle segment of the closing ring (401) is a conical body which is attached to the inner wall of the funnel-shaped partition block (103); under the action of the spring force, the edge of the conical body of the middle segment of the closing ring (401) is deformed to tightly attach to the conical inner wall of the funnel-shaped partition block (103), so as to realize liquid sealing; the taper angle of the conical body of the middle segment of the closing ring (401) is greater than the taper angle of the funnel-shaped partition block (103), so that the reaction reagent is sealed in the upper end of the closing ring (401).

6. The high throughput reaction tube of claim 5, wherein: The lower segment of the closing ring (401) is a straight cylinder, the outer diameter of the straight cylinder is smaller than the size of the small-diameter straight hole of the tube body, and there is a liquid discharge gap; the closing ring (401) is installed in cooperation with the stepped column at the top end of the valve core (402) through a ring-shaped stepped groove, and in the installation process, the stepped column at the top end of the valve core is installed into the ring-shaped stepped groove of the closing ring through elastic deformation of the closing ring (401); the pull-out force between the closing ring (401) and the valve core (402) after installation is greater than the spring force.

7. The high throughput reaction tube of claim 6, wherein: The diameter of the step cylinder at the bottom of the valve core (402) is smaller than the diameter of the valve core assembly chamber of the tube body, so that the liquid flows out from the peripheral gap; The outer end surface of the bottom step cylinder of the valve core (402) is provided with a central tapered hole, which is aligned with the central position of the valve core (402) when the external lifting device at the bottom of the reaction tube opens the valve core; A plurality of through grooves are circumferentially arranged on the outer cylindrical surface of the bottom step of the valve core (402) for draining water; The top step cylinder of the valve core is provided with a rounded edge, which facilitates the installation of the guide during the assembly process with the sealing ring (401); The valve core (402) is made of a material that is compatible with the reaction liquid.

8. The high throughput reaction tube of claim 6, wherein: The spring (403) provides a sealing compression force for the sealing ring (401), and the spring (403) is a helical linear spring; the spring (403) is made of stainless steel.

9. The high throughput reaction tube of claim 1, wherein: When the tube body (1) is used to discharge reagents from the upper end, the lower end of the tube body (1) is a closed flat bottom, and the flat bottom is suitable for a mixing device with a heating magnet; The inner wall of the tube body (1) is also provided with protrusions for increasing the rotation force of the liquid when rotating in the mixing device.

10. The high throughput reaction tube of any one of claims 1-9, wherein: A plurality of the reaction tubes are arranged on a 96-well plate to form a high-throughput reaction well plate.

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