Cell impurity removal vessel

Abstract

The utility model discloses a cell impurity removal vessel which comprises a culture dish body and a culture dish cover, three partition plates are arranged in the culture dish body and divide the culture dish body into three fan-shaped areas, one fan-shaped area is set as a cleaning area, the interior of the cleaning area is divided into a smooth part and a cleaning part, and the smooth part and the cleaning part are arranged in the culture dish body. And concave-convex lines are arranged on the cleaning part. The cell sap can be stored in a partitioned mode, granular cells can be rapidly scraped off from oocytes through the concave-convex lines in the cleaning area during operation, and the cleaning work efficiency of the oocytes is effectively improved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture vessel technology, and in particular to a cell impurity removal vessel. Background Technology

[0002] In in-vitro fertilization (IVF), mature oocytes are aspirated from the ovary into a test tube using negative pressure suction. The test tube contains a mixture of oocytes and follicular fluid, which at this stage contains red blood cells, white blood cells, granulosa cells, and sometimes blood clots. Oocytes in this state cannot be used directly for IVF. Before IVF, the oocytes need to be cleaned to remove impurities, and the granulosa cells need to be pre-treated by removing some layers of granulosa cells. The specific procedure is as follows: Embryology lab personnel use an oocyte-collecting needle to pick up the oocytes (including granulosa cells) from the follicular fluid, scrape them against the bottom of a culture dish, and wash them several times in fertilization fluid to remove some granulosa cells and blood clots. However, the oocyte-collecting dishes and culture dishes currently used are flat plates with smooth surfaces, making it difficult to scrape off the granulosa cells. Furthermore, the entire oocyte pre-treatment process is performed under a microscope, which is extremely time-consuming and labor-intensive. To improve the efficiency of oocyte cleaning and impurity removal, this application provides a cell impurity removal device. Utility Model Content

[0003] This invention provides a cell impurity removal vessel that can store cell fluid in separate sections. During operation, the textured surface of the cleaning zone can quickly scrape granulosa cells off the oocytes, effectively improving the efficiency of oocyte cleaning.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0005] A cell impurity removal device includes a culture dish body and a culture dish lid. The culture dish body is provided with three partitions, dividing the culture dish body into three fan-shaped areas. One of the fan-shaped areas is designated as a cleaning area. The cleaning area is divided into a smooth part and a cleaning part. The cleaning part is provided with concave and convex textures.

[0006] Furthermore, the top of the arc-shaped inner wall of the cleaning area and the bottom of the culture dish body are beveled, and the bevel extends to the middle of the cleaning area to form the cleaning part; the convex and concave textures are densely distributed on the beveled surface of the bevel.

[0007] Furthermore, the petri dish body is made of a transparent material.

[0008] Furthermore, the raised and recessed texture is set as a grid knurled texture.

[0009] Furthermore, the raised and recessed textures are configured as several fan-shaped arc textures parallel to the inner arc contour of the cleaning area.

[0010] The beneficial effects of this utility model are:

[0011] 1) This invention can set three fan-shaped areas on the culture dish body, which correspond to the washing area, the treatment area, and the clean area respectively. Follicular fluid containing red blood cells, white blood cells, granulosa cells, and oocytes is placed in the treatment area. During treatment, the oocytes to be treated are transferred to the washing area. The oocytes are extracted using an oocyte retrieval needle, and the textured surface of the washing area is used to gently scrape them to quickly remove the granulosa cells adhering to the oocytes. The smooth part of the washing area is then observed under a microscope to confirm whether the cleaning is complete, effectively improving the efficiency of oocyte washing. The cleaned oocytes are transferred to the clean area, where fertilization fluid can be placed.

[0012] 2) The cleaning section is designed with an incline. When transferring oocytes, the liquid can be poured onto the incline. The flowing liquid passes through the concave and convex texture, which can block and filter out some impurities, thus improving the impurity removal efficiency. Attached Figure Description

[0013] The specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings, wherein:

[0014] Figure 1 This is a perspective view of the overall structure of this utility model;

[0015] Attached image labels:

[0016] 1—Cultural dish body, 2—Cultural dish lid, 11—Separator, 12—Smooth part, 13—Washing part. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] It should be noted that when a component is described as "fixed to" another component, it can be directly on the other component or may have a central component. When a component is described as "connected to" another component, it can be directly connected to the other component or may have a central component. When a component is described as "set on" another component, it can be directly set on the other component or may have a central component. When a component is described as "set in the middle," it is not simply set in the exact center, as long as it is not set within the area defined by both ends being in the middle. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0020] Reference Figure 1 As shown, a cell impurity removal device includes a culture dish body 1 and a culture dish lid 2. The culture dish body 1 is made of transparent material and has three partitions 11 inside, dividing it into three fan-shaped areas. One of these fan-shaped areas is designated as a cleaning area, which is further divided into a smooth section 12 and a cleaning section 13. The cleaning section 13 has a textured surface. This invention allows the culture dish body to be divided into three fan-shaped areas, corresponding to a cleaning area, a treatment area, and a clean area, respectively. Follicular fluid containing red blood cells, white blood cells, granulosa cells, and oocytes is placed in the treatment area. During treatment, the oocytes to be processed are transferred to the cleaning area. Oocytes are extracted using an oocyte-collecting needle, and the textured surface of the cleaning area is used to gently scrape the oocytes, quickly removing granulosa cells. The smooth section of the cleaning area is then observed under a microscope to confirm whether the impurities have been completely removed, effectively improving the efficiency of oocyte cleaning. The cleaned oocytes are then transferred to the clean area, where fertilization fluid can be placed. In practical use, the treatment area and the clean area can be clearly marked, for example, by using a colored pen to mark the bottom of the petri dish.

[0021] Furthermore, the top of the arc-shaped inner wall of the cleaning zone and the bottom of the culture dish body 1 are beveled, and the bevel extends to the middle of the cleaning zone to form the cleaning section 13; the concave and convex textures are densely arranged on the beveled surface. When transferring oocytes, the liquid can be poured onto the bevel, and the flowing liquid can pass through the concave and convex textures, which can block and filter out some impurities, thereby improving the impurity removal efficiency.

[0022] As one preferred embodiment, the textured surface is designed as a knurled grid pattern. This design allows the operator to slide the oocyte from any direction to scrape it and remove the granulosa cells adhering to it.

[0023] As another preferred option, the textured surface is configured as several fan-shaped arc patterns parallel to the inner arc contour of the cleaning area. The concave and convex shapes of the fan-shaped arc patterns are staggered and concentrically arranged. This design forms horizontally spaced patterns, which can also effectively slow down and block the flow of follicular fluid.

[0024] The above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Any modifications or equivalent substitutions that do not depart from the spirit and scope of this utility model should be covered within the scope of the technical solution of this utility model.

Claims

1. A cell impurity removal device, comprising a culture dish body and a culture dish lid, characterized in that, The culture dish body is provided with three partitions, which divide the culture dish body into three fan-shaped areas. One of the fan-shaped areas is designated as a cleaning area, which is divided into a smooth part and a cleaning part. The cleaning part is provided with concave and convex textures.

2. The cell impurity removal vessel according to claim 1, characterized in that, The top of the arc-shaped inner wall of the cleaning area and the bottom of the culture dish body are beveled, and the bevel extends to the middle of the cleaning area to form the cleaning part; the concave and convex textures are densely distributed on the beveled surface of the bevel.

3. The cell impurity removal vessel according to claim 2, characterized in that, The petri dish body is made of transparent material.

4. The cell impurity removal vessel according to claim 2, characterized in that, The raised and recessed texture is designed as a grid knurled pattern.

5. The cell impurity removal vessel according to claim 2, characterized in that, The raised and recessed texture is configured as several fan-shaped arc textures parallel to the inner arc contour of the cleaning area.

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