Strain protection culture dish with DNA watermark

Abstract

The utility model discloses a strain protection culture dish with DNA (deoxyribonucleic acid) watermarks, which relates to the technical field of strain culture and comprises a support column, three groups of support tables arranged at equal intervals and three groups of protection plates arranged at equal intervals are mounted on the outer side of the support column, a partition plate is mounted below the protection plates, and a culture dish component is movably mounted at the top of each support table; the culture dish assembly comprises a dish body movably installed on the top of the supporting table, and a DNA watermark layer is installed on the bottom wall of the inner side of the dish body. According to the culture dish, the DNA watermark layer is installed, the DNA watermark layer combines a material containing a specific DNA watermark sequence with a dish body material through a special process, the DNA watermark sequence is associated with cultured strain information, in the strain culture process, the identity and source of a strain can be confirmed by reading the DNA watermark layer through an identification reading card, and if it is found that the DNA watermark is abnormal, the culture dish can be used as a culture dish. And measures can be taken in time to prevent illegal acquisition or use of strains.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of strain culture equipment, specifically to strain protection culture dish with DNA watermark. BACKGROUND

[0002] In synthetic biology, the strain culture process often faces the problem of strain loss or pollution caused by illegal acquisition or misoperation. Traditional culture dishes cannot effectively identify and protect the source of the strain, while the DNA watermark technology can provide an effective solution, but there is currently a lack of corresponding special culture dishes.

[0003] Patent document CN216808794U discloses a culture dish for strain culture, which discloses "a culture dish for strain culture, the culture dish for strain culture, including a culture box, further comprising: a culture dish, the culture box is slidably provided with a turntable inside, a plurality of culture dishes for cultivating bacterial groups are placed on the turntable; a top cover is provided on the top of the culture box, a light supplementing lamp and an ultraviolet lamp are installed on the inner side wall of the top cover, a plurality of fixing holes are formed in the side wall of the culture box, a fixing pin is provided on the side wall of the top cover, and one end of the fixing pin penetrates into the fixing hole".

[0004] However, the technical solution disclosed in the above-mentioned document lacks a structure design for assisting in identifying strain sample information, which can easily cause the appearance of illegal samples. UTILITY MODEL CONTENT

[0005] The utility model aims to provide a strain protection culture dish with DNA watermark to solve the technical problem of lacking a structure design for assisting in identifying strain sample information in the background art, which can easily cause the appearance of illegal samples.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a strain protection culture dish with DNA watermark, comprising: a support column, three groups of equidistantly arranged support tables are installed on the outer side of the support column, three groups of equidistantly arranged protection plates are installed on the outer side of the support column, a partition plate is installed below the protection plate, the partition plate is installed at the bottom of the protection plate, and a culture dish assembly is movably installed on the top of the support table.

[0007] The culture dish assembly comprises a dish body movably installed on the top of the support table, a DNA watermark layer is installed on the inner side bottom wall of the dish body, a dish cover is movably installed on the top of the dish body, a clamping piece is installed on the bottom of the dish cover, a sealing gasket is installed on the outer side of the clamping piece, and the sealing gasket abuts against the inner side of the dish body.

[0008] Preferably, a ventilation opening is formed in the inner side of the dish cover.

[0009] Preferably, the inner side of the dish cover is movably provided with a rotating shaft, the outer side of the rotating shaft is provided with a hand grip, the outer side of the rotating shaft is provided with four groups of symmetrically distributed baffles, and the four groups of baffles are located above and below the air vent respectively.

[0010] Preferably, the outer wall of the dish body is provided with a placing groove.

[0011] Preferably, the inner side of the placing groove is movably provided with an identification reading card.

[0012] Preferably, the outer wall of the dish body is provided with a handle, and the handle is located below the placing groove.

[0013] Preferably, the bottom of the supporting column is provided with a bottom plate.

[0014] Compared with the prior art, the present application has the following beneficial effects:

[0015] 1. The DNA watermark layer is installed, the DNA watermark layer combines the material containing the specific DNA watermark sequence with the dish body material through a special process, the DNA watermark sequence is associated with the information of the cultured strain, in the strain culture process, the identity and source of the strain can be confirmed by reading the DNA watermark layer through the identification reading card, if the DNA watermark is found to be abnormal, measures can be taken in time to prevent illegal acquisition or use of the strain.

[0016] 2. The sealing gasket is installed, the bottom of the dish cover is fixedly connected with the clamping piece, the clamping piece is kept stable, the clamping piece fixes the outer side sealing gasket, the sealing gasket is kept stable, the sealing gasket abuts against the inside of the dish body, the sealing property is ensured, external pollution is prevented, the inner side rotating shaft of the dish cover is supported, the rotating shaft can be stably rotated, the outer side hand grip of the rotating shaft is fixed, the user grips the hand grip to drive the rotating shaft to rotate, the rotating shaft rotates to drive the outer side baffle to rotate, the baffle is blocked above the air vent, the culture dish is closed, the normal growth of the strain is ensured, when air exchange is needed, the baffle is rotated to stagger above and below the air vent. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a whole structure schematic view of the present application;

[0018] Figure 2 It is a cross-sectional structure schematic view of the present application;

[0019] Figure 3 It is a dish body structure schematic view of the present application;

[0020] Figure 4 It is a dish cover structure schematic view of the present application.

[0021] In the diagram: 1. Support column; 2. Support platform; 3. Partition; 4. Protective plate; 5. Base plate; 6. Dish body; 7. DNA watermark layer; 8. Handle; 9. Placement slot; 10. Identification card; 11. Sealing gasket; 12. Clip; 13. Dish lid; 14. Rotating shaft; 15. Vent; 16. Baffle; 17. Hand grip. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0024] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0025] Please see Figure 1 , Figure 2 , Figure 3 and Figure 4 A microbial culture dish with DNA watermark protection includes: a support column 1, three sets of equidistant support platforms 2 installed on the outside of the support column 1, three sets of equidistant protective plates 4 installed on the outside of the support column 1, a partition 3 installed below the protective plate 4, the partition 3 installed at the bottom of the protective plate 4, a culture dish assembly movably installed on the top of the support platform 2, and a base plate 5 installed at the bottom of the support column 1.

[0026] The base plate 5 is fixed to the top support column 1 to ensure the stability of the support column 1. The support column 1 is fixed to the outer support platform 2 and the protective plate 4 to ensure the stability of the support platform 2 and the protective plate 4. The support platform 2 supports the top petri dish assembly, making it convenient for users to install the petri dish assembly and ensuring the stability of the petri dish assembly so that the petri dish assembly can stably cultivate bacteria.

[0027] The culture dish assembly includes a dish body 6 movably mounted on the top of the support platform 2, wherein the dish body 6 is movably connected to the top of the support platform 2 via a slot; a DNA watermark layer 7 is installed on the inner bottom wall of the dish body 6; a dish lid 13 is movably mounted on the top of the dish body 6, wherein the dish lid 13 is movably connected to the top of the dish body 6 via a snap-fit ​​connection; a retainer 12 is installed at the bottom of the dish lid 13, and a sealing gasket 11 is installed on the outer side of the retainer 12 for sealing. The gasket 11 rests against the inner side of the dish body 6; the inner side of the dish lid 13 is provided with a vent 15, and the vent 15 extends through the thickness direction of the dish lid 13. The vent 15 communicates with the inner cavity of the dish body 6 and is used for gas exchange during the culture process; a rotating shaft 14 is movably installed through the inner side of the dish lid 13, a handle 17 is installed on the outer side of the rotating shaft 14, and four sets of symmetrically distributed baffles 16 are installed on the outer side of the rotating shaft 14, with the four sets of baffles 16 located above and below the vent 15 respectively;

[0028] The support platform 2 supports the top dish 6, which provides a suitable growth environment for the bacteria. The dish 6 is made of a material with high transparency, high temperature resistance, and chemical corrosion resistance. A vent 15 is opened on the inner side of the dish lid 13 for gas exchange. The bottom of the dish lid 13 is fixedly connected to the clip 12, which remains stable. The clip 12 fixes the outer sealing gasket 11, which remains stable and rests against the inside of the dish 6 to ensure airtightness and prevent external contamination. The dish lid 13 supports the inner rotating shaft 14, ensuring that the rotating shaft 14 can rotate smoothly. The rotating shaft 14 is fixed to the outer handle 17. When the user grips the handle 17, the rotating shaft 14 rotates. The rotation of the rotating shaft 14 causes the outer baffle 16 to rotate. The baffle 16 blocks the vent 15, sealing the culture dish and ensuring normal growth of the bacteria. When air exchange is needed, the baffle 16 can be rotated to offset the vent 15 from above and below.

[0029] The outer wall of the dish body 6 is provided with a placement groove 9, and an identification card 10 is movably installed on the inner side of the placement groove 9. The outer wall of the dish body 6 is provided with a handle 8, and the handle 8 is located below the placement groove 9.

[0030] The dish body 6 is fixed to the outer placement slot 9 to ensure the stability of the placement slot 9. The placement slot 9 limits the inner identification and reading card 10, and the identification and reading card 10 facilitates the identification device to read the DNA watermark information.

[0031] Specifically, the DNA watermark layer 7 uses a special process to combine material containing a specific DNA watermark sequence with the dish material. The material containing the DNA watermark sequence is a short-chain DNA molecule that is artificially synthesized. Its sequence contains coding related to the bacterial strain information, including the source laboratory number of the bacterial strain, the strain type code, and the collection time information. These short-chain DNA molecules can be modified, such as by adding chemical groups to the ends, to enhance their binding ability with the dish material.

[0032] Furthermore, during the bacterial culture process, the identity and origin of the bacterial strain can be confirmed by reading the DNA watermark layer 7 using the identification reader 10. If an abnormality in the DNA watermark is detected, timely measures can be taken to prevent the illegal acquisition or use of the bacterial strain. Its working principle is as follows:

[0033] The identification and reading card operates based on nucleic acid hybridization technology. The card contains oligonucleotide probes complementary to specific DNA sequences in the DNA watermark layer. These probes are specially labeled, such as with fluorescent labels (using fluorescent groups like FITC, Cy3, etc.). When the identification and reading card comes into contact with a dish containing the DNA watermark layer, the probes hybridize with the target DNA sequence in the watermark layer. If hybridization is successful, the label emits a corresponding signal (fluorescent signal). The presence and characteristics of the DNA watermark sequence are determined by detecting these signals. The reading operation steps are as follows:

[0034] 1) Place the reading card: Remove the identification reading card from the placement slot on the outer wall of the culture dish, ensuring that the surface of the reading card is clean and free of impurities. Then, gently place the detection side of the reading card (i.e., the side containing the probe) onto the DNA watermark layer at the bottom of the dish, ensuring full contact between the two.

[0035] 2) Device Reading: Use a specialized reading device, such as a portable fluorescence detector. Place the culture dish with the reading card attached inside the detector's detection chamber and close the chamber door. Turn on the detector; the device will emit excitation light of a specific wavelength, exciting the marker on the probe to emit a signal.

[0036] 3) Signal Processing: The photoelectric sensor in the detector receives the emitted signal (such as fluorescence signal intensity) and converts it into an electrical signal. The built-in signal processing software then analyzes the electrical signal, comparing the signal intensity with a preset threshold. If the signal intensity is higher than the threshold, it indicates successful detection of the DNA watermark sequence. Further analysis of the DNA sequence information is performed based on signal characteristics (such as fluorescence spectrum), comparing it with pre-stored bacterial strain-related DNA watermark sequence information in the device.

[0037] 4) Comparison Analysis: Normal DNA watermark sequence information for each culture dish is pre-stored in the database, including complete sequence data and possible minor variations (such as the allowable range of individual base changes due to experimental errors or natural degradation). When reading the DNA watermark layer, the actual detected sequence is compared with the standard sequence in the database. If sequence length discrepancies (difference exceeding 5%), base mismatch rates exceeding a preset threshold (e.g., 3%), or errors are found in key information encoding regions (e.g., the laboratory number area of ​​the strain origin) are detected, the DNA watermark is deemed abnormal.

[0038] The overall working principle of this utility model is as follows: The support platform 2 supports the top dish 6, which provides a suitable growth environment for the microorganisms. The dish 6 is composed of a material with high transparency, high temperature resistance, and chemical corrosion resistance. A vent 15 is opened on the inner side of the dish lid 13 for gas exchange. The bottom of the dish lid 13 is fixedly connected to the clip 12, which remains stable. The clip 12 fixes the outer sealing gasket 11, ensuring stability. The sealing gasket 11 rests against the inside of the dish 6 to ensure airtightness and prevent external contamination. The dish lid 13 supports the inner rotating shaft 14, ensuring... The rotating shaft 14 can rotate smoothly. The rotating shaft 14 is fixed to the outer gripper 17. When the user grips the gripper 17, it drives the rotating shaft 14 to rotate. The rotation of the rotating shaft 14 drives the outer baffle 16 to rotate. The baffle 16 blocks the air vent 15 and seals the culture dish to ensure the normal growth of the bacteria. When air exchange is needed, the baffle 16 can be rotated to be offset from above and below the air vent 15. The dish body 6 is fixed to the outer placement groove 9 to ensure the stability of the placement groove 9. The placement groove 9 limits the inner identification card 10. The identification card 10 facilitates the identification device to read the DNA watermark information.

[0039] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A microbial culture dish with a DNA watermark, characterized in that, Includes: a support column (1), three sets of equally spaced support platforms (2) installed on the outside of the support column (1), three sets of equally spaced protective plates (4) installed on the outside of the support column (1), a partition (3) installed below the protective plate (4), the partition (3) installed at the bottom of the protective plate (4), and a petri dish assembly movably installed on the top of the support platform (2); The culture dish assembly includes a dish body (6) movably mounted on the top of a support platform (2), a DNA watermark layer (7) is installed on the inner bottom wall of the dish body (6), a dish lid (13) is movably mounted on the top of the dish body (6), a clip (12) is installed at the bottom of the dish lid (13), and a sealing gasket (11) is installed on the outer side of the clip (12), with the sealing gasket (11) abutting against the inner side of the dish body (6).

2. The bacterial strain protection culture dish with DNA watermark according to claim 1, characterized in that: The inner side of the lid (13) of the dish is provided with a vent (15).

3. The bacterial strain protection culture dish with DNA watermark according to claim 1, characterized in that: A rotating shaft (14) is movably installed through the inner side of the lid (13). A hand grip (17) is installed on the outer side of the rotating shaft (14). Four sets of symmetrically distributed baffles (16) are installed on the outer side of the rotating shaft (14), and the four sets of baffles (16) are located above and below the vent (15) respectively.

4. The bacterial strain protection culture dish with DNA watermark according to claim 1, characterized in that: The outer wall of the dish (6) is fitted with a placement groove (9).

5. The bacterial strain protection culture dish with DNA watermark according to claim 4, characterized in that: An identification card (10) is movably installed on the inner side of the placement slot (9).

6. The bacterial strain protection culture dish with DNA watermark according to claim 4, characterized in that: The outer wall of the dish (6) is fitted with a handle (8), and the handle (8) is located below the placement slot (9).

7. The bacterial strain protection culture dish with DNA watermark according to claim 1, characterized in that: The bottom of the support column (1) is fitted with a base plate (5).

Images