Frozen ovum unfreezing device

Abstract

The utility model belongs to the field of bioengineering, and particularly relates to a frozen ovum thawing device which comprises a thawing reagent box for thawing frozen ovum, a shell and a control module, the device comprises a shell, the top of the shell is sleeved with a cover plate, a conveying mechanism is arranged on the cover plate, an ovum carrying tube is arranged on the conveying mechanism, an electric push rod is fixed in the shell, the output end of the electric push rod is fixedly provided with a placing plate, the placing plate is provided with three resuscitation reagent boxes, and the three resuscitation reagent boxes correspond to the ovum carrying tube. A control module is fixedly installed on the shell. The electric push rod and the conveying mechanism are both connected with the control module. And the conveying mechanism comprises transmission rollers, a transmission belt, a moving plate and a motor, a mounting groove is formed in the cover plate, and the two transmission rollers are rotationally connected into the mounting groove. According to the utility model, the frozen ovum can be automatically unfrozen and resuscitated through the matching of all the parts.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, and in particular to a device for thawing frozen eggs. Background Technology

[0002] Thawing frozen eggs mainly involves two processes: rewarming and removing the cryoprotectant, ultimately restoring their potential for continued development. Rewarming generally uses a rapid heating rate to avoid the formation of ice crystals that could damage the embryo. When embryos are frozen, they are permeated with a high concentration of cryoprotectant, which needs to be removed promptly after thawing to allow water to replace the cryoprotectant and permeate into the cells. Therefore, the thawing agent contains some non-permeable cryoprotectant, allowing the embryo to gradually absorb water and expel the cryoprotectant from the embryonic cells. This prevents drastic changes in osmotic pressure across the cell membrane caused by rapid water absorption, which could damage the embryo.

[0003] However, with the development of assisted reproductive technology, reproductive centers perform a large number of embryo, oocyte and sperm thawing operations every day, which is a lot of work. During thawing, the cryopreservation tubes are taken out of the liquid nitrogen tank, and then different thawing fluids are taken out one by one to prepare for thawing. This process is time-consuming and complicated, which greatly reduces the work efficiency of the thawing staff. The staff have to run back and forth between the incubator and the test table, which will inevitably affect the precise control of the thawing temperature and time, and thus affect the success rate of embryo thawing.

[0004] Therefore, we propose a frozen egg thawing device to address the problems in the background art. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a frozen egg thawing device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A frozen oocyte thawing device includes a thawing reagent box for thawing frozen oocytes, a housing, and a control module;

[0008] The top of the shell is fitted with a cover plate, the cover plate is equipped with a conveying mechanism, the conveying mechanism is equipped with an oocyte carrier tube, an electric push rod is fixed inside the shell, the output end of the electric push rod is fixedly installed with a placement plate, the placement plate is equipped with three resuscitation reagent boxes, each corresponding to the oocyte carrier tube, a control module is fixedly installed on the shell, and the electric push rod and the conveying mechanism are both connected to the control module;

[0009] The conveying mechanism includes a transmission roller, a transmission belt, a moving plate, and a motor. The cover plate has an installation groove in which two transmission rollers are rotatably connected. Through the coordinated design of the control module, electric push rod, conveying mechanism, thawing reagent box, and oocyte carrier tube, the frozen oocytes can be automatically thawed and thawed, greatly improving convenience and efficiency.

[0010] Specifically, the two drive rollers are connected to the same drive belt, and multiple moving plates are fixedly installed on the drive belt for intermittently moving and conveying the egg carrier tube. Each of the multiple moving plates has through holes, and the egg carrier tube is placed in the through holes for easy placement.

[0011] Specifically, an electric motor is fixedly installed on the cover plate, and the output end of the electric motor is fixedly connected to one end of one of the two transmission rollers for driving the entire conveying mechanism. The electric motor is connected to the control module, and the control module drives and controls the electric motor.

[0012] Specifically, a vibration motor is fixedly installed on the cover plate. The vibration motor is connected to the control module. The vibration motor causes the cover plate to vibrate, which in turn causes the oocyte carrier tube to vibrate. This not only improves the revival efficiency of the revival reagent on the frozen oocytes, but also prevents the revival reagent attached to the oocyte carrier tube from dripping onto the placement plate.

[0013] Specifically, the placement plate has three grooves, and the three resuscitation reagent boxes are placed in the three grooves respectively to limit the position of the three resuscitation reagent boxes.

[0014] Specifically, each of the three recovery reagent boxes contains a different oocyte thawing reagent, and the three recovery reagent boxes are respectively equipped with thawing solution TS, diluent DS and washing solution WS.

[0015] Specifically, the cover plate is provided with a pick-and-place slot, which corresponds to multiple movable plates and three resuscitation reagent boxes, making it convenient for staff to pick up and place the oocyte carrier tube.

[0016] Specifically, a retaining ring is fixedly sleeved on the oocyte carrier tube, and the retaining ring is in contact with the corresponding movable plate to limit the position of the oocyte carrier tube.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0018] (1) The frozen oocyte thawing device of this utility model, through the coordinated design of the control module, electric push rod, conveying mechanism, revival reagent box and oocyte carrier tube, can achieve automatic thawing and revival of frozen oocytes, which greatly improves convenience and efficiency.

[0019] (2) The frozen oocyte thawing device of this utility model can cause the cover plate to vibrate and the oocyte carrier tube to vibrate through the action of the vibration motor. This not only improves the thawing efficiency of the thawing reagent on the frozen oocyte, but also prevents the thawing reagent attached to the oocyte carrier tube from dripping onto the placement plate. Attached Figure Description

[0020] To more clearly illustrate the embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary. The structures, proportions, sizes, etc., shown in this specification are only used to complement the content disclosed in the specification for those skilled in the art to understand and read, and are not intended to limit the conditions under which this utility model can be implemented. Therefore, they have no substantial technical significance, and any modification of the structure, change of the proportional relationship, or adjustment of the size is not permitted.

[0021] Figure 1 This is a three-dimensional structural diagram of a frozen egg thawing device proposed in this utility model;

[0022] Figure 2 This is an anatomical diagram of a frozen egg thawing device proposed in this utility model;

[0023] Figure 3 for Figure 2 Enlarged structural diagram at point A;

[0024] Figure 4 This is an exploded structural diagram of a frozen egg thawing device proposed in this utility model;

[0025] Figure 5 This is a bottom view of the cover plate structure of the frozen egg thawing device proposed in this utility model;

[0026] Figure 6 This is a schematic diagram of the conveying mechanism of a frozen egg thawing device proposed in this utility model.

[0027] In the diagram: 1. Shell; 2. Cover plate; 3. Vibration motor; 4. Conveying mechanism; 5. Oocyte carrier tube; 6. Control module; 7. Electric push rod; 8. Placement plate; 9. Resuscitation reagent box; 10. Mounting slot; 41. Drive roller; 42. Drive belt; 43. Moving plate; 44. Electric motor. Detailed Implementation

[0028] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0029] Reference Figures 1-6 A frozen oocyte thawing device includes a thawing reagent box 9 for thawing frozen oocytes, a housing 1, and a control module 6;

[0030] The top of the shell 1 is fitted with a cover plate 2, the cover plate 2 is equipped with a conveying mechanism 4, the conveying mechanism 4 is equipped with an oocyte carrier tube 5, an electric push rod 7 is fixed inside the shell 1, the output end of the electric push rod 7 is fixedly installed with a placement plate 8, the placement plate 8 is equipped with three resuscitation reagent boxes 9, each corresponding to the oocyte carrier tube 5, and a control module 6 is fixedly installed on the shell 1, the electric push rod 7 and the conveying mechanism are both connected to the control module 6;

[0031] The conveying mechanism 4 includes a transmission roller 41, a transmission belt 42, a moving plate 43, and a motor 44. The cover plate 2 has an installation groove 10, in which two transmission rollers 41 are rotatably connected. Through the coordinated design of the control module 6, the electric push rod 7, the conveying mechanism 4, the revival reagent box 9, and the oocyte carrier tube 5, the frozen oocytes can be automatically thawed and revived, greatly improving convenience and efficiency.

[0032] In this embodiment, the same transmission belt 42 is connected to the two transmission rollers 41. Multiple moving plates 43 are fixedly installed on the transmission belt 42 for intermittently moving and conveying the egg carrier tube 5. Each of the multiple moving plates 43 has through holes, and the egg carrier tube 5 is placed in the through holes for easy placement.

[0033] In this embodiment, a motor 44 is fixedly installed on the cover plate 2. The output end of the motor 44 is fixedly connected to one end of one of the two transmission rollers 41, which is used to drive the entire conveying mechanism 4. The motor 44 is connected to the control module 6, and the control module 6 drives and controls the motor 44.

[0034] In this embodiment, a vibration motor 3 is fixedly installed on the cover plate 2. The vibration motor 3 is connected to the control module 6. The vibration motor 3 can cause the cover plate 2 to vibrate, which in turn causes the oocyte carrier tube 5 to vibrate. This not only improves the revival efficiency of the revival reagent on the frozen oocytes, but also prevents the revival reagent attached to the oocyte carrier tube 5 from dripping onto the placement plate 8.

[0035] In this embodiment, the placement plate 8 has three grooves, and the three resuscitation reagent boxes 9 are placed in the three grooves respectively to limit the position of the three resuscitation reagent boxes 9. Each of the three resuscitation reagent boxes 9 is equipped with a heating plate and a temperature sensor. The three heating plates and temperature sensors are all connected to the control module 6. The heating plates and temperature sensors are common in daily life and belong to the prior art. Those skilled in the art can implement them, so they will not be described in detail.

[0036] In this embodiment, the three resuscitation reagent boxes 9 are each equipped with different oocyte thawing reagents, and the three resuscitation reagent boxes 9 are respectively equipped with thawing solution TS, diluent DS and washing solution WS1.

[0037] In this embodiment, the cover plate 2 is provided with a pick-and-place slot, which corresponds to multiple movable plates 43 and three resuscitation reagent boxes 9, making it convenient for staff to pick up and place the oocyte carrier tube 5.

[0038] In this embodiment, a retaining ring is fixedly sleeved on the oocyte carrier tube 5, and the retaining ring is in contact with the corresponding moving plate 43 to limit the oocyte carrier tube 5. The oocyte carrier tube 5 is provided with a grid to facilitate the thawing, dilution and cleaning of frozen oocytes with thawing fluid. It also facilitates the removal of thawed oocytes by staff through glass capillary tubes. The specific structure and working principle of the oocyte carrier tube 5 can be referred to the literature with application number: 20211106363.9. This is prior art and will not be described in detail.

[0039] Working principle: During use, the control module 6 drives and controls the electric push rod 7, the electric motor 44, and the vibration motor 3. At the same time, the control module 6 drives and controls the heating plates in the three resuscitation reagent boxes 9, thereby achieving the effect of heating the resuscitation reagent. Meanwhile, the temperature sensors in the three resuscitation reagent boxes 9 can monitor the temperature of the resuscitation reagent in real time, thus facilitating the adjustment of the resuscitation reagent temperature.

[0040] First, the frozen oocyte carrier tube 5 is placed in the through hole on the moving plate 43. Then, the oocyte carrier tube 5 is aligned with one of the three thawing reagent boxes 9. Then, driven by the electric push rod 7 and the transmission of the placement plate 8, the three thawing reagent boxes 9 are moved upward and the oocyte carrier tube 5 is immersed into the corresponding thawing reagent box 9. Then, the frozen oocytes in the oocyte carrier tube 5 are thawed and thawed by the thawing reagent in the thawing reagent box 9.

[0041] Simultaneously, the corresponding transmission roller 41 rotates on the cover plate 2 through the drive of the motor 44. At the same time, through the cooperation between the two transmission rollers 41 and the transmission belt 42, the transmission belt 42 drives multiple moving plates 43 to rotate and move intermittently, thereby causing the multiple moving plates 43 to move the oocyte carrier tubes 5 synchronously, thus positioning the oocyte carrier tubes 5 sequentially with the three resuscitation reagent boxes 9. Simultaneously, under the cooperation between the electric push rod 7 and the motor 44, the oocyte carrier tubes 5 are sequentially immersed into the three resuscitation reagent boxes 9, thereby achieving the automatic operation of thawing, diluting and cleaning the frozen oocytes in the oocyte carrier tubes 5 sequentially.

[0042] Meanwhile, the vibration motor 3 causes the cover plate 2 to vibrate, which in turn causes the oocyte carrier tube 5 to vibrate. This not only improves the revival efficiency of the revival reagent on the frozen oocytes, but also prevents the revival reagent attached to the oocyte carrier tube 5 from dripping onto the placement plate 8.

[0043] The technological advancement of this invention compared to the prior art is that, through the cooperation of various components, it can achieve automatic thawing and revival of frozen eggs, improving convenience and efficiency, and its structure is simple and more practical.

Claims

1. A frozen oocyte thawing apparatus, characterized by, The shell (1) is provided with a recovery reagent box (9) for thawing frozen ova, a housing (1) and a control module (6). The top of the shell (1) is provided with a cover plate (2), the cover plate (2) is provided with a conveying mechanism (4), the conveying mechanism (4) is provided with an ovum carrier tube (5), the shell (1) is fixed with an electric push rod (7), the output end of the electric push rod (7) is fixedly installed with a placing plate (8), the placing plate (8) is provided with three recovery reagent boxes (9) and corresponds to the ovum carrier tube (5), the shell (1) is fixedly installed with a control module (6), and the electric push rod (7) and the conveying mechanism are connected with the control module (6). The conveying mechanism (4) comprises a transmission roller (41), a transmission belt (42), a moving plate (43) and a motor (44), the cover plate (2) is provided with a mounting groove (10), and the mounting groove (10) is rotatably connected with two transmission rollers (41).

2. A frozen egg thawing apparatus according to claim 1, wherein The same transmission belt (42) is connected to the two transmission rollers (41), a plurality of moving plates (43) are fixedly installed on the transmission belt (42), a plurality of through holes are formed in the moving plates (43), and the ovum carrier tube (5) is placed in the through holes.

3. A frozen oocyte thawing device according to claim 2, wherein The cover plate (2) is fixedly installed with a motor (44), one end of the transmission roller (41) is fixedly connected with the output end of the motor (44), and the motor (44) is connected with the control module (6).

4. The device for thawing frozen ova according to claim 1, wherein The cover plate (2) is fixedly installed with a vibration motor (3), and the vibration motor (3) is connected with the control module (6).

5. The device for thawing frozen ova according to claim 1, wherein The placing plate (8) is provided with three recesses, and the three recovery reagent boxes (9) are respectively placed in the three recesses.

6. The device for thawing frozen ova according to claim 1, wherein The three recovery reagent boxes (9) are respectively provided with different ovum thawing reagents.

7. The device for thawing frozen ova according to claim 1, wherein The cover plate (2) is provided with a taking and placing groove, and the taking and placing groove corresponds to the plurality of moving plates (43) and the three recovery reagent boxes (9).

8. The device for thawing frozen ova according to claim 1, wherein The ovum carrier tube (5) is fixedly provided with a blocking ring, and the blocking ring is in contact with the corresponding moving plate (43). The ovum carrier tube (5) is fixedly provided with a blocking ring, and the blocking ring is in contact with the corresponding moving plate (43).

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