Pulmonary arterial hypertension experiment auxiliary mechanism

Abstract

The utility model relates to the technical field of pulmonary arterial hypertension experiments, and discloses a pulmonary arterial hypertension experiment auxiliary mechanism which comprises a base. According to the auxiliary mechanism for the pulmonary arterial hypertension experiment, through the arrangement of the pushing mechanism and the clamping mechanism, when a mouse cage containing a rat is fixed, a power source is switched on, an electric push rod on the inner wall of a test bin is started, the electric push rod drives a placement plate at the tail end to move on a bottom limiting sliding rail, and after the placement plate is moved to a proper position, the mouse cage is fixed; a worker can conveniently place a mouse cage in four mouse cage placing openings in the surface of the placing plate, then pull clamping plates on the two sides of the mouse cage placing openings to extrude torsion springs on the two sides, and loosen the clamping plates after the mouse cage is placed, so that the clamping plates are subjected to opposite acting force provided by the two sides to firmly fix the mouse cage; the rat is prevented from violating in the test process, so that the collapse of the rat cage or the accidental injury of the rat is avoided, and the normal test is not influenced.

Description

Technical Field

[0001] This utility model relates to the field of pulmonary hypertension testing technology, and in particular to an auxiliary mechanism for pulmonary hypertension testing. Background Technology

[0002] Pulmonary hypertension (PH), a serious cardiovascular disease threatening human health, has a complex pathogenesis involving multiple levels such as genetics, immunology, and molecular biology, attracting widespread attention from the global medical community in recent years. To gain a deeper understanding and overcome this challenge, various experimental support institutions and technologies for pulmonary hypertension have emerged. These aim to simulate the internal environment of the human body, explore disease progression, evaluate treatment strategies, and provide a solid theoretical foundation for clinical practice.

[0003] A rat model of pulmonary hypertension was established using a hypoxic method. Rats were randomly divided into four groups: normoxic group (N group), hypoxic group (H group), low-concentration rAAV treatment group (H+L-rAAV group), and high-concentration rAAV treatment group (H+H-rAAV group). The mean pulmonary arterial hypertension (mPAP) and pulmonary vascular resistance (ΔPVR), right ventricular remodeling index RV / (LV+S), and morphological changes and remodeling indices of pulmonary small vessels, including the percentage of wall thickness (WT%) and the percentage of wall area (WA%), were measured in each group. After sacrificing the rats, lung tissue was collected from each group of rats. The expression levels of SLC7A11-mRNA and SGLT1-mRNA were detected by qRT-PCR. The expression levels of SLC7A11, SGLT1, PCNA, and Bax were detected by Western blotting. Actin was detected by Western blotting to observe whether disulfide bonds were formed between actin proteins, and to evaluate the therapeutic effect of rAAV on pulmonary hypertension through the disulfide death pathway.

[0004] Existing experimental support mechanisms for pulmonary hypertension typically require securing the rat cages to prevent rats from agitating during the experiment, causing the cages to collapse or resulting in accidental injury to the rats, thus affecting the normal conduct of the experiment. Therefore, an experimental support mechanism for pulmonary hypertension is proposed. Utility Model Content

[0005] (a) Technical problems to be solved

[0006] The purpose of this invention is to provide an auxiliary mechanism for pulmonary hypertension experiments, which solves the problem mentioned in the background art of preventing rats from agitating during the experiment, causing the rat cage to collapse or the rats to be accidentally injured, thus affecting the normal conduct of the experiment.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, this utility model provides the following technical solution: a pulmonary hypertension experimental auxiliary mechanism, comprising a base, a support platform fixedly connected to the top of the base, an experimental chamber fixedly connected to the top of the support platform, a pushing mechanism fixedly connected to the inner wall of the experimental chamber, a placement plate fixedly connected to the end of the pushing mechanism, four mouse cage placement openings on the surface of the placement plate, clamping mechanisms fixedly connected to both sides of the surface of each mouse cage placement opening, an adjustment mechanism connected to the upper surface of the experimental chamber, an oxygen concentration detector mounted on the surface of the experimental chamber, and the pushing mechanism comprising a component fixedly connected to the experimental chamber. The inner wall has an electric push rod, one side of which is electrically connected to a battery via a power cord; the clamping mechanism includes a fixed cylinder fixedly connected to both sides of the surface of the cage placement opening, a torsion spring fixedly connected inside the fixed cylinder, a rotating cylinder fixedly connected to one end of the torsion spring, and a clamping plate fixedly connected to the surface of the rotating cylinder; the adjustment mechanism includes an air pipe connected to the upper surface of the test chamber, a mixing chamber connected to the bottom of the air pipe, a diverter pipe connected to both sides of the mixing chamber, a gas flow meter installed on the surface of the diverter pipe, an electrically controlled valve installed on the lower surface of the diverter pipe, and a suction fan installed inside the air pipe.

[0009] As a further embodiment of this utility model, the surface of the clamping plate is provided with several anti-slip grooves, and the rotating cylinder is rotatably connected to the inside of the fixed cylinder. The anti-slip grooves provide an anti-slip function.

[0010] As a further embodiment of this utility model, the bottom of one of the diversion pipes is connected to an oxygen tank, and the bottom of the other diversion pipe is connected to a nitrogen tank. The diversion pipes serve to transport gases.

[0011] As a further embodiment of this invention, a PLC controller is electrically connected to one side of the electrically controlled valve via a power cord. The PLC controller is installed on the surface of the test chamber, and the test chamber serves to conduct experiments on rats.

[0012] As a further embodiment of this utility model, a pressure gauge is electrically connected to one side of the PLC controller via a power cord. The pressure gauge is installed on the top of the test chamber, and its function is to detect the internal pressure of the test chamber.

[0013] As a further embodiment of this utility model, a pressure relief port is provided on the top surface of the test chamber, and a pressure relief valve is installed on the lower surface of the pressure relief port. The pressure relief valve serves to relieve pressure.

[0014] As a further embodiment of this utility model, the bottom of the placement plate has two sliding grooves, and the interior of the sliding grooves is slidably connected to a limiting slide rail. The limiting slide rail serves to limit the placement plate.

[0015] (III) Beneficial Effects

[0016] This utility model provides an auxiliary mechanism for pulmonary hypertension testing, which has the following beneficial effects:

[0017] 1. This pulmonary hypertension experimental auxiliary mechanism, through the setting of a pushing mechanism and a clamping mechanism, when fixing the rat cage containing the rat, the power is turned on and the electric push rod on the inner wall of the experimental chamber is activated. The electric push rod drives the placement plate at the end to move on the bottom limit slide rail until the placement plate is moved to the appropriate position, so that the staff can place the rat cage in the four rat cage placement openings on the surface of the placement plate. Then, the clamping plates on both sides of the rat cage placement opening are pulled, so that the clamping plates compress the torsion springs on both sides. After the rat cage is placed, the clamping plates are released. The clamping plates are subjected to the opposite force provided by both sides, so that the clamping plates firmly fix the rat cage, preventing the rat from agitating during the experiment, causing the rat cage to collapse or the rat to be accidentally injured, which would affect the normal progress of the experiment.

[0018] 2. This pulmonary hypertension experimental auxiliary mechanism, through the adjustment mechanism, can open the electrically controlled valve on the surface of the shunt tube, allowing oxygen and nitrogen to enter the mixing chamber through the pipeline. Then, the suction fan inside the mixing chamber delivers them to the experimental chamber. The oxygen and nitrogen content is precisely controlled by the gas flow meter installed on the shunt tube, and in conjunction with the oxygen concentration detector installed on the surface of the experimental chamber, the oxygen concentration inside the experimental chamber is precisely controlled, improving the accuracy of experimental testing and the reliability of data. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the adjustment mechanism of this utility model;

[0021] Figure 3 This is a schematic diagram of the propulsion mechanism of this utility model;

[0022] Figure 4 This is a schematic diagram of the clamping mechanism of this utility model.

[0023] In the diagram: 1. Base; 2. Support platform; 3. Test chamber; 4. Pushing mechanism; 401. Electric push rod; 402. Battery; 5. Placement plate; 6. Rat cage placement opening; 7. Clamping mechanism; 701. Fixed cylinder; 702. Torsion spring; 703. Rotating cylinder; 704. Clamping plate; 8. Adjustment mechanism; 801. Gas pipe; 802. Mixing box; 803. Diverter pipe; 804. Gas flow meter; 805. Electrically controlled valve; 806. Suction fan; 9. Oxygen concentration detector; 10. Oxygen tank; 11. Nitrogen tank; 12. PLC controller; 13. Pressure gauge; 14. Pressure relief port; 15. Pressure relief valve; 16. Limiting slide rail. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0025] Please see Figures 1 to 4 This utility model provides a technical solution: an auxiliary mechanism for pulmonary hypertension experiments, including a base 1, a support platform 2 fixedly connected to the top of the base 1, an experimental chamber 3 fixedly connected to the top of the support platform 2, a pushing mechanism 4 fixedly connected to the inner wall of the experimental chamber 3, and a clamping mechanism 7 to prevent rats from agitating during the experiment, causing the rat cage to collapse or the rats to be accidentally injured, thus affecting the normal progress of the experiment. A placement plate 5 is fixedly connected to the end of the pushing mechanism 4, and four rat cage placement openings 6 are opened on the surface of the placement plate 5. Clamping mechanisms 7 are fixedly connected to both sides of the surface of the rat cage placement openings 6. An adjustment mechanism 8 is connected to the upper surface of the experimental chamber 3, which improves the accuracy of experimental detection and the reliability of data. An oxygen concentration detector 9 is installed on the surface of the experimental chamber 3. The pushing mechanism 4 includes an electric push rod 401 fixedly connected to the inner wall of the test chamber 3, and a battery 402 is electrically connected to one side of the electric push rod 401 via a power cord; the clamping mechanism 7 includes a fixed cylinder 701 fixedly connected to both sides of the surface of the mouse cage placement opening 6, a torsion spring 702 fixedly connected inside the fixed cylinder 701, a rotating cylinder 703 fixedly connected to one end of the torsion spring 702, and a clamping plate 704 fixedly connected to the surface of the rotating cylinder 703; the adjusting mechanism 8 includes an air pipe 801 connected to the upper surface of the test chamber 3, a mixing box 802 connected to the bottom of the air pipe 801, a diversion pipe 803 connected to both sides of the mixing box 802, a gas flow meter 804 installed on the surface of the diversion pipe 803, an electric control valve 805 installed on the lower surface of the diversion pipe 803, and a suction fan 806 installed inside the air pipe 801.

[0026] The surface of the clamping plate 704 is provided with several anti-slip grooves. The rotating cylinder 703 is rotatably connected to the inside of the fixed cylinder 701. The anti-slip grooves play an anti-slip role.

[0027] One of the shunt pipes 803 is connected to an oxygen tank 10 at the bottom, and the other shunt pipe 803 is connected to a nitrogen tank 11 at the bottom. The shunt pipes 803 are designed to transport gases.

[0028] One side of the electric control valve 805 is electrically connected to a PLC controller 12 via a power cord. The PLC controller 12 is installed on the surface of the test chamber 3. The test chamber 3 serves to conduct experiments on rats.

[0029] A pressure gauge 13 is electrically connected to one side of the PLC controller 12 via a power cord. The pressure gauge 13 is installed on the top of the test chamber 3. The pressure gauge 13 is used to detect the internal pressure of the test chamber 3.

[0030] The top surface of the test chamber 3 is provided with a pressure relief port 14, and a pressure relief valve 15 is installed on the lower surface of the pressure relief port 14. The pressure relief valve 15 is used to relieve pressure.

[0031] The bottom of the placement plate 5 has two grooves, and the inside of the grooves is connected to a limiting slide rail 16. The limiting slide rail 16 is used to limit the placement plate 5.

[0032] In this invention, the working steps of the device are as follows:

[0033] First step: When fixing the rat cage containing the rat, turn on the power and start the electric push rod 401 on the inner wall of the test chamber 3. The electric push rod 401 drives the placement plate 5 at the end to move on the bottom limit slide rail 16 until the placement plate 5 is moved to a suitable position, so that the staff can place the rat cage in the four rat cage placement openings 6 on the surface of the placement plate 5. Then pull the clamping plates 704 on both sides of the rat cage placement opening 6, so that the clamping plates 704 squeeze the torsion springs 702 on both sides. After the rat cage is placed, release the clamping plates 704. The clamping plates 704 are subjected to the opposite force provided by both sides, so that the clamping plates 704 firmly fix the rat cage.

[0034] The second step: The electrically controlled valve 805 on the surface of the diversion pipe 803 can be opened, allowing oxygen and nitrogen to enter the mixing chamber 802 through the pipe. The suction fan 806 inside the mixing chamber 802 then delivers the oxygen to the test chamber 3. The gas flow meter 804 on the diversion pipe 803 precisely controls the oxygen and nitrogen content, and the oxygen concentration detector 9 on the surface of the test chamber 3 further assists in precisely controlling the oxygen concentration inside the test chamber 3. It should be noted that the device structure and accompanying drawings mainly describe the principle of this utility model. The power mechanism, power supply system, and control system of the device are not fully described in detail. However, those skilled in the art, understanding the principle of the above utility model, can clearly understand the specific details of its power mechanism, power supply system, and control system. The control method in the application document is automatic control via a controller, and the controller's control circuit can be implemented through simple programming by those skilled in the art.

[0035] All standard parts used can be purchased from the market, and can be customized according to the instructions and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the existing technology. The machinery, parts and equipment adopt conventional models in the existing technology, and the structure and principle of the components known to those skilled in the art can be known by those skilled in the art through technical manuals or conventional experimental methods.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A pulmonary hypertension experimental auxiliary mechanism, comprising a base (1), characterized in that: A support platform (2) is fixedly connected to the top of the base (1), and a test chamber (3) is fixedly connected to the top of the support platform (2). A pushing mechanism (4) is fixedly connected to the inner wall of the test chamber (3), and a placement plate (5) is fixedly connected to the end of the pushing mechanism (4). Four rat cage placement openings (6) are opened on the surface of the placement plate (5). Clamping mechanisms (7) are fixedly connected to both sides of the surface of the rat cage placement openings (6). An adjustment mechanism (8) is connected to the upper surface of the test chamber (3), and an oxygen concentration detector (9) is installed on the surface of the test chamber (3). The pushing mechanism (4) includes an electric push rod (401) fixedly connected to the inner wall of the test chamber (3), and a battery (402) is electrically connected to one side of the electric push rod (401) via a power line. The clamping mechanism (7) includes a fixed cylinder (701) fixedly connected to both sides of the surface of the mouse cage placement opening (6). A torsion spring (702) is fixedly connected inside the fixed cylinder (701). A rotating cylinder (703) is fixedly connected to one end of the torsion spring (702). A clamping plate (704) is fixedly connected to the surface of the rotating cylinder (703). The regulating mechanism (8) includes an air pipe (801) connected to the upper surface of the test chamber (3), a mixing box (802) connected to the bottom of the air pipe (801), a diverter pipe (803) connected to both sides of the mixing box (802), a gas flow meter (804) installed on the surface of the diverter pipe (803), an electric control valve (805) installed on the lower surface of the diverter pipe (803), and a suction fan (806) installed inside the air pipe (801).

2. The pulmonary hypertension experimental auxiliary mechanism according to claim 1, characterized in that: The surface of the clamping plate (704) is provided with several anti-slip grooves, and the rotating cylinder (703) is rotatably connected to the inside of the fixed cylinder (701).

3. The pulmonary hypertension experimental auxiliary mechanism according to claim 1, characterized in that: One of the shunt tubes (803) is connected to an oxygen tank (10) at its bottom, and the other shunt tube (803) is connected to a nitrogen tank (11) at its bottom.

4. The pulmonary hypertension experimental auxiliary mechanism according to claim 1, characterized in that: One side of the electric control valve (805) is electrically connected to a PLC controller (12) via a power line, and the PLC controller (12) is installed on the surface of the test chamber (3).

5. The pulmonary hypertension experimental auxiliary mechanism according to claim 4, characterized in that: A pressure gauge (13) is electrically connected to one side of the PLC controller (12) via a power line, and the pressure gauge (13) is installed on the top of the test chamber (3).

6. The pulmonary hypertension experimental auxiliary mechanism according to claim 1, characterized in that: The test chamber (3) has a pressure relief port (14) on its top surface, and a pressure relief valve (15) is installed on the lower surface of the pressure relief port (14).

7. The pulmonary hypertension experimental auxiliary mechanism according to claim 1, characterized in that: The bottom of the placement plate (5) has two sliding grooves, and the inside of the sliding grooves is slidably connected to a limit rail (16).

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