DAC fraction collecting device in cap analogue synthesis

Abstract

The utility model provides a DAC fraction collecting device in cap analogue synthesis. The DAC fraction collecting device comprises a bottom plate, supporting plates symmetrically arranged at the bottom of the bottom plate, a fixing cavity formed in the surface of a placing pipe and used for placing a test tube, a groove formed in the top of the bottom plate, a heating pipe fixedly connected in the groove, and fan blades evenly arranged on the surface of a rotating shaft. A rotating shaft is driven by a first motor to rotate, a placing pipe is driven to rotate under the action of the rotating shaft, so that a DAC mixed solution in cap analogue synthesis placed in a fixed cavity is centrifuged, layering of the mixed solution is facilitated, during re-centrifugation, a sealing cover covers the upper surface of a bottom plate, and during centrifugation, a heating pipe is started to heat air in the bottom plate; when the rotating shaft rotates, the fan blades rotate along with the rotating shaft to drive air in the sealing cover to flow, so that DAC mixed liquid in the fixing cavity is heated more uniformly, the heating speed is high, the heating pipe does not rotate along with the rotating shaft, the service life is long, and use is convenient.

Description

Technical Field

[0001] This utility model relates to the field of DAC fraction collection technology, specifically to a DAC fraction collection device in cap analogue synthesis. Background Technology

[0002] Cap analogs are chemically modified RNA cap structures primarily used in the study of RNA metabolism and the development of novel drugs. Cap analogs typically contain one or more thiophosphate groups, the presence of which significantly affects RNA translation efficiency, binding affinity, and nuclear stability. The synthesis of cap analogs is quite complex, usually requiring multiple reaction steps, and the reagents and starting materials used are not readily available.

[0003] Chinese patent application CN202321136753.6 discloses an automatic fraction collection device, including a housing, a test tube rack, and a heating tube. A fixed frame is fixedly installed at the bottom center of the housing. A groove is fixedly opened at the center of the inner wall of the fixed frame. Two sliders are slidably installed on the inner wall of the groove, and the two sliders are connected to the test tube rack. A heating tube is fixedly installed at the bottom of the test tube rack. A second groove is fixedly opened at the bottom of the housing opposite the fixed frame. A second motor is fixedly installed at the bottom of the second groove. The output end of the second motor passes through the fixed frame and is fixedly connected to the test tube rack. This device has the function of rapidly collecting proteins in a mixture. Simultaneously, this automatic fraction collection device also has the function of collecting water vapor generated during fractionation to prevent water vapor from damaging the internal electronic components of the device.

[0004] However, the following drawbacks still exist:

[0005] When heating the mixture, heat will dissipate into the chamber, resulting in heat loss, slow heating speed, and the heating tube is easily damaged as it rotates with the test tube rack. Utility Model Content

[0006] The present invention aims to solve the problems mentioned in the background art by providing a DAC fraction collection device in the synthesis of cap analogues.

[0007] The specific technical solution is as follows:

[0008] A DAC fraction collection device for the synthesis of cap analogues includes: a base plate, with support plates symmetrically arranged at the bottom of the base plate, a control switch fixedly connected to one side of the base plate, a first motor fixedly connected to the center of the bottom of the base plate, a rotating shaft fixedly connected to the output shaft end of the first motor, a fixing frame evenly distributed on the surface of the rotating shaft, a placement tube fixedly connected to the surface of the fixing frame, a fixing cavity for placing test tubes opened on the surface of the placement tube, a sealing cover provided on the base plate, a groove opened on the top of the base plate, a heating tube fixedly connected in the groove, and fan blades evenly arranged on the surface of the rotating shaft.

[0009] The DAC fraction collection device in the above-mentioned cap analog synthesis, wherein: the surface of the placement tube is uniformly provided with through holes, and the placement tube is set as a metal tube.

[0010] In the DAC fraction collection device for the above-mentioned cap analogue synthesis, the diameter of the groove is greater than twice the length of the fan blade, and the fan blade is positioned above the heating tube.

[0011] In the DAC fraction collection device for the above-mentioned cap analogue synthesis, a sealing groove is provided on the top of the base plate, and the sealing cover is inserted into the sealing groove.

[0012] In the DAC fraction collection device for the above-mentioned cap analogue synthesis, the sealing cover is configured as a transparent cover, and a sealing strip is fixedly connected to the sealing groove.

[0013] The DAC fraction collection device in the above-mentioned cap analog synthesis includes: a lifting assembly on the base plate, the lifting assembly including a mounting frame fixedly connected to one side of the base plate, a sliding groove on the mounting frame, a second motor fixedly installed at the bottom of the mounting frame, a lead screw rotatably connected in the sliding groove, the output shaft end of the second motor fixedly connected to the lead screw, a sliding plate slidably connected in the sliding groove, the sliding plate being threadedly connected to the lead screw, and a sealing cover fixedly connected to the bottom of the sliding plate.

[0014] In the DAC fraction collection device for the above-mentioned cap analog synthesis, a vacuum pump is fixedly installed at the bottom of the base plate, a connecting pipe is fixedly connected to the vacuum pump, and the connecting pipe is fixedly connected to the sealing cover.

[0015] This utility model has the following beneficial effects:

[0016] The first motor drives the rotating shaft to rotate, which in turn drives the placement tube to rotate, thereby centrifuging the DAC mixture in the cap-like synthesis placed in the fixed cavity. This facilitates the stratification of the mixture. During centrifugation, the sealing cover is placed on the upper surface of the base plate. During centrifugation, the heating tube is activated to heat the air inside the base plate. As the rotating shaft rotates, the fan blades follow the rotation, driving the airflow inside the sealing cover. This makes the DAC mixture in the fixed cavity more evenly heated, with a fast heating speed. Furthermore, the heating tube does not rotate with the rotating shaft, resulting in a long service life and ease of use. Attached Figure Description

[0017] Figure 1 A schematic diagram of a DAC fraction collection device in the synthesis of cap analogues provided in this embodiment of the present invention;

[0018] Figure 2 A schematic diagram of the rotating shaft, fixing frame, and placement tube of a DAC fraction collection device in the synthesis of cap analogues provided in this embodiment of the present invention;

[0019] Figure 3 This is a schematic diagram of the lifting assembly in a DAC fraction collection device for the synthesis of cap analogues, provided by an embodiment of the present invention.

[0020] Figure 4 This is an enlarged schematic diagram of the sealing cover in a DAC fraction collection device for the synthesis of cap analogs provided in this embodiment of the present invention.

[0021] In the attached image:

[0022] 1. Base plate; 2. Support plate; 3. First motor; 4. Rotating shaft; 5. Fixing frame; 6. Placement tube; 7. Fixing cavity; 8. Through hole; 9. Control switch; 10. Groove; 11. Heating tube; 12. Fan blade; 13. Sealing cover; 14. Lifting assembly; 1401. Mounting bracket; 1402. Slide groove; 1403. Second motor; 1404. Lead screw; 1405. Sliding plate; 15. Vacuum pump; 16. Connecting pipe; 17. Sealing groove; 18. Sealing strip. Detailed Implementation

[0023] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.

[0024] The accompanying drawings are for illustrative purposes only and are schematic diagrams, not actual images. They should not be construed as limiting the scope of this patent. To better illustrate the embodiments of this utility model, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual dimensions of the product. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.

[0025] In the accompanying drawings of this utility model, the same or similar reference numerals correspond to the same or similar components. In the description of this utility model, it should be understood that if terms such as "upper," "lower," "left," "right," "inner," and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0026] In the description of this utility model, unless otherwise explicitly specified and limited, the term "connection" or similar designation indicating the connection relationship between components should be interpreted broadly. For example, it can refer to a fixed connection, a detachable connection, or an integral part; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0027] Example

[0028] This embodiment provides a DAC fraction collection device for the synthesis of cap analogues, such as... Figures 1-4 As shown, it includes: a base plate 1, support plates 2 symmetrically arranged at the bottom of the base plate 1, a control switch 9 fixedly connected to one side of the base plate 1, a first motor 3 fixedly connected at the center of the bottom of the base plate 1, a rotating shaft 4 fixedly connected to the output shaft end of the first motor 3, a fixing frame 5 evenly distributed on the surface of the rotating shaft 4, a placement tube 6 fixedly connected to the surface of the fixing frame 5, a fixing cavity 7 for placing test tubes opened on the surface of the placement tube 6, a sealing cover 13 provided on the base plate 1, a groove 10 opened on the top of the base plate 1, a heating tube 11 fixedly connected in the groove 10, and fan blades 12 evenly arranged on the surface of the rotating shaft 4.

[0029] A DAC fraction collection device for cap analog synthesis using the above-mentioned technical solution is configured such that a first motor 3 drives a rotating shaft 4 to rotate, which in turn drives a placement tube 6 to rotate, thereby centrifuging the DAC mixture in the cap analog synthesis placed in a fixed cavity 7, which facilitates the stratification of the mixture. During centrifugation, a sealing cover 13 is placed on the upper surface of the bottom plate 1. During centrifugation, a heating tube 11 is activated to heat the air inside the bottom plate 1. When the rotating shaft 4 rotates, the fan blades 12 rotate with the rotating shaft 4, driving the airflow inside the sealing cover 13, making the DAC mixture in the fixed cavity 7 more evenly heated, with a fast heating speed. The heating tube 11 does not rotate with the rotating shaft 4, has a long service life, and is easy to use.

[0030] To ensure that through holes 8 are evenly opened on the surface of the tube 6, the tube 6 is made of metal.

[0031] In order to ensure that the diameter of the groove 10 is greater than twice the length of the fan blade 12, the fan blade 12 is positioned above the heating tube 11.

[0032] In order to improve the sealing effect between the sealing cover 13 and the base plate 1 and increase the heating speed, a sealing groove 17 is provided on the top of the base plate 1, and the sealing cover 13 is inserted into the sealing groove 17.

[0033] To facilitate observation of the layering inside the sealing cover 13, the sealing cover 13 is designed to be transparent, and a sealing strip 18 is fixedly connected to the sealing groove 17. The sealing strip 18 further enhances the sealing performance with the sealing cover 13.

[0034] To move the sealing cover 13, a lifting assembly 14 is provided on the base plate 1. The lifting assembly 14 includes a mounting bracket 1401 fixedly connected to one side of the base plate 1. A sliding groove 1402 is provided on the mounting bracket 1401. A second motor 1403 is fixedly installed at the bottom of the mounting bracket 1401. A lead screw 1404 is rotatably connected in the sliding groove 1402. The output shaft end of the second motor 1403 is fixedly connected to the lead screw 1404. A sliding plate 1405 is slidably connected in the sliding groove 1402. The sliding plate 1405 is threadedly connected to the lead screw 1404. The sealing cover 13 is fixedly connected to the bottom of the sliding plate 1405. When the DAC fraction in the synthesis of cap analogues is required, the DAC mixture test tube in the synthesis of cap analogues is placed into the fixed cavity 7, and the second motor 1403 is started to drive the lead screw 1404 to rotate, thereby causing the sliding plate 1405 to move the sealing cover 13 downward, so that the sealing cover 13 is inserted into the sealing groove 17, and the sealing cover 13 is sealed with the bottom plate 1, which facilitates rapid heating.

[0035] In order to extract the water vapor generated after centrifugal heating, a vacuum pump 15 is fixedly installed at the bottom of the base plate 1. A connecting pipe 16 is fixedly connected to the vacuum pump 15, and the connecting pipe 16 is fixedly connected to the sealing cover 13.

[0036] Among them, the control switch 9 is electrically connected to the first motor 3, the second motor 1403 and the vacuum pump 15 respectively, and is used to control the operation of the first motor 3, the second motor 1403 and the vacuum pump 15.

[0037] In summary, the DAC fraction collection device for cap analog synthesis provided in this embodiment has the following advantages: the first motor 3 drives the rotating shaft 4 to rotate, which in turn drives the placement tube 6 to rotate, thereby centrifuging the DAC mixture for cap analog synthesis placed in the fixed cavity 7, which facilitates the stratification of the mixture. During centrifugation, the sealing cover 13 is placed on the upper surface of the bottom plate 1. During centrifugation, the heating tube 11 is activated to heat the air in the bottom plate 1. When the rotating shaft 4 rotates, the fan blade 12 rotates with the rotating shaft 4, driving the air flow in the sealing cover 13, making the DAC mixture in the fixed cavity 7 more evenly heated and faster. Moreover, the heating tube 11 does not rotate with the rotating shaft 4, has a long service life, and is easy to use.

[0038] In use, when the DAC fraction from the cap analogue synthesis needs to be separated, the DAC mixture test tube from the cap analogue synthesis is placed in the fixed cavity 7. The second motor 1403 is activated by the control switch 9, driving the lead screw 1404 to rotate. This causes the sliding plate 1405 to move the sealing cover 13 downwards, inserting the sealing cover 13 into the sealing groove 17, thus sealing the sealing cover 13 with the base plate 1. The first motor 3 is activated by the control switch 9, driving the rotating shaft 4 to rotate. The rotating shaft 4 then drives the placement tube 6 to rotate, thereby centrifuging the DAC mixture from the cap analogue synthesis placed in the fixed cavity 7, facilitating the stratification of the mixture. During centrifugation, the fan blade 12 rotates along with the rotating shaft 4, causing airflow within the sealed cover 13. This ensures more uniform heating of the DAC mixture within the fixed chamber 7 and accelerates the heating process. The vacuum pump 15 is activated via the control switch 9, drawing water vapor from the sealed cover 13 out through the connecting pipe 16 to complete fractionation. After fractionation, the sealed cover 13 is raised, and the rotating shaft 4 is activated again, rotating the fan blade 12 to generate airflow and cool the heating tube 11 and the placement tube 6. This facilitates the handling of the test tube containing the DAC mixture.

[0039] The above are merely preferred embodiments of the present utility model and are not intended to limit the implementation methods and protection scope of the present utility model. Those skilled in the art should realize that any equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A DAC fraction collection device for the synthesis of cap analogues, characterized in that, include: A base plate (1) is provided with a support plate (2) symmetrically arranged at the bottom of the base plate (1). A control switch (9) is fixedly connected to one side of the base plate (1). A first motor (3) is fixedly connected to the center of the bottom of the base plate (1). A rotating shaft (4) is fixedly connected to the output shaft end of the first motor (3). Fixing frames (5) are evenly distributed on the surface of the rotating shaft (4). A placement tube (6) is fixedly connected to the surface of the fixing frame (5). A fixing cavity (7) for placing test tubes is opened on the surface of the placement tube (6). A sealing cover (13) is provided on the base plate (1). A groove (10) is opened on the top of the base plate (1). A heating tube (11) is fixedly connected in the groove (10). Fan blades (12) are evenly arranged on the surface of the rotating shaft (4).

2. The DAC fraction collection device for the synthesis of cap analogues according to claim 1, characterized in that, The surface of the placement tube (6) is uniformly provided with through holes (8), and the placement tube (6) is made of metal.

3. The DAC fraction collection device for the synthesis of cap analogues according to claim 1, characterized in that, The diameter of the groove (10) is greater than twice the length of the fan blade (12), which is positioned above the heating tube (11).

4. The DAC fraction collection device for the synthesis of cap analogues according to claim 1, characterized in that, The bottom plate (1) has a sealing groove (17) on its top, and the sealing cover (13) is inserted into the sealing groove (17).

5. The DAC fraction collection device for the synthesis of cap analogues according to claim 4, characterized in that, The sealing cover (13) is configured as a transparent cover, and a sealing strip (18) is fixedly connected to the sealing groove (17).

6. The DAC fraction collection device for the synthesis of cap analogues according to claim 1, characterized in that, A lifting assembly (14) is provided on the base plate (1). The lifting assembly (14) includes a mounting bracket (1401) fixedly connected to one side of the base plate (1). A sliding groove (1402) is provided on the mounting bracket (1401). A second motor (1403) is fixedly installed at the bottom of the mounting bracket (1401). A lead screw (1404) is rotatably connected in the sliding groove (1402). The output shaft end of the second motor (1403) is fixedly connected to the lead screw (1404). A sliding plate (1405) is slidably connected in the sliding groove (1402). The sliding plate (1405) is threadedly connected to the lead screw (1404). The sealing cover (13) is fixedly connected to the bottom of the sliding plate (1405).

7. The DAC fraction collection device for the synthesis of cap analogues according to any one of claims 1-6, characterized in that, A vacuum pump (15) is fixedly installed at the bottom of the base plate (1), and a connecting pipe (16) is fixedly connected to the vacuum pump (15). The connecting pipe (16) is fixedly connected to the sealing cover (13).

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