Diagnosis and treatment integrated PDT instrument

Abstract

The utility model discloses a diagnosis and treatment integrated PDT instrument which comprises multi-wavelength regulation and control laser equipment and an imaging mechanism. The multi-wavelength regulation and control laser equipment comprises a fluorescence excitation laser device for emitting laser to enable a photosensitizer on the tissue to generate fluorescence and a photodynamic therapy laser device for emitting laser with multiple wavelengths to kill tumor cells on the tissue; the imaging mechanism comprises a camera device, an image processing module and a display, the camera device is used for obtaining a color image and a fluorescence image of the tissue, the image processing module is connected with the camera device, and the display is connected with the image processing module. According to the utility model, sequential implementation of tumor fluorescence labeling and photodynamic therapy is facilitated, the purpose of integration of diagnosis and treatment is achieved, and the combined characteristic of a photosensitizer-laser illumination mode for realizing fluorescence labeling and laser tumor cell killing for photodynamic therapy is determined.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of medical apparatuses, more particularly, relates to a diagnosis and treatment integrated PDT instrument. BACKGROUND

[0002] Photodynamic therapy (PDT) technology is a kind of drug and instrument combined technology, which is a photosensitization reaction with oxygen molecules participating in and accompanying biological effects. After photosensitizer is injected into the body of a patient, relatively high accumulation is formed in the tissue, and the photosensitizer is excited by the laser irradiation of the diseased tissue of a specific wavelength, oxygen molecules are transferred by energy, free radicals and singlet oxygen and other active oxygen are generated, cytotoxicity is generated, and the treatment method is to cause tumor cells to be damaged and even die.

[0003] PDT is a promising antitumor therapy, and as an independent treatment method, it has certain effect on early central lung cancer, central airway obstruction and patients with advanced pleural disease. Overall, photodynamic therapy is gradually becoming an important treatment method in clinic as a non-invasive, efficient and low-toxicity treatment method.

[0004] At present, PDT includes the application of photosensitizer, the accumulation of photosensitizer molecules in target tissue and the irradiation of suitable visible light source.

[0005] In photodynamic therapy, the wavelength, power density and energy density of light have a great influence on the effect of treatment, and the required laser wavelength for excitation is different for different manufacturers of photosensitizer. At present, the model of the fluorescent agent used on the tissue is often a single-mode laser that emits laser of a set wavelength to excite fluorescence and irradiate for a certain time. However, sometimes this way of photodynamic therapy cannot achieve good treatment effect. UTILITY MODEL CONTENT

[0006] In view of the above defects or improvement needs of the prior art, the utility model provides a diagnosis and treatment integrated PDT instrument, which can observe the fluorescence labeling and photodynamic tumor cell killing effect of the PDT instrument under different irradiation modes, analyze the immune action mechanism of PDT treatment, determine the combination characteristics of photosensitizer-laser light irradiation mode for realizing fluorescence labeling and laser irradiation for killing tumor cells, so as to achieve better photodynamic treatment effect.

[0007] To achieve the above purpose, according to the utility model, a diagnosis and treatment integrated PDT instrument is provided, which comprises a multi-wavelength control laser device and an imaging mechanism, characterized in that,

[0008] The multi-wavelength regulation laser device comprises a fluorescence excitation laser device for emitting laser to make photosensitizer on the tissue generate fluorescence and a photodynamic therapy laser device for emitting laser of multiple wavelengths to kill tumor cells on the tissue.

[0009] The imaging mechanism comprises a camera connected with the image processing module and a display connected with the image processing module.

[0010] Preferably, the fluorescence excitation laser devices are multiple and each is a multimode laser, and the fluorescence excitation laser devices emit laser of different wavelengths.

[0011] Preferably, each of the fluorescence excitation laser devices has an optical fiber interface A.

[0012] The diagnosis and treatment integrated PDT instrument further comprises an optical fiber coupler A having multiple laser inlets A and one laser outlet A, and each of the laser inlets A is connected with one of the optical fiber interfaces A.

[0013] Preferably, the controller is connected with the fluorescence excitation laser devices through signal lines to make the fluorescence excitation laser devices emit laser of set wavelengths.

[0014] Preferably, the photodynamic therapy laser devices are multiple and each is a single-mode laser, and the photodynamic therapy laser devices emit laser of different wavelengths.

[0015] Preferably, each of the photodynamic therapy laser devices has an optical fiber interface B.

[0016] The diagnosis and treatment integrated PDT instrument further comprises an optical fiber coupler B having multiple laser inlets B and one laser outlet B, and each of the laser inlets B is connected with one of the optical fiber interfaces B.

[0017] Preferably, the diagnosis and treatment integrated PDT instrument further comprises a tumor diagnosis and treatment device, and the tumor diagnosis and treatment device comprises a photodynamic irradiation catheter and an implantation catheter used in cooperation, and a photodynamic interface on a photodynamic irradiation optical fiber built in a tube of the photodynamic irradiation catheter is connected with the laser outlet B.

[0018] Preferably, the controller is connected with the photodynamic therapy laser devices through signal lines to make the photodynamic therapy laser devices emit laser of set wavelengths and set light-emitting modes, wherein the light-emitting modes include continuous light, long-pulse light and short-pulse light, and the controller is installed with a switch for controlling the opening and closing of the laser and is further installed with a mode selection button for controlling the light-emitting modes of the laser.

[0019] Preferably, the fluorescence excitation laser device and / or the photodynamic therapy laser device satisfies the following conditions: output power ≥ 2W, output pulse width is 1ms-999ms, power instability ≤ 5%, and reproducibility ≤ 5%;

[0020] The optical fiber interface A and / or the optical fiber interface B satisfies the following conditions: in line with international standard SMA905 and core diameter ≥ 400um.

[0021] Preferably, a test bench for placing the tissue is further included.

[0022] Overall, compared with the prior art, the above technical scheme conceived by the utility model can achieve the following beneficial effects:

[0023] 1) The diagnosis and treatment integrated PDT instrument of the utility model can excite fluorescence through the fluorescence excitation laser device, mark and diagnose the position of tumor cells, and the photodynamic therapy laser device emits laser beams of multiple wavelengths, can realize single wavelength output or multiple wavelength mixed output, can kill tumor cells through laser irradiation of the photodynamic therapy laser device, and can obtain color and fluorescence images of the tissue through the camera device, which facilitates sequential implementation of tumor fluorescence marking and photodynamic therapy, and achieves the purpose of diagnosis and treatment integration.

[0024] 2) The diagnosis and treatment integrated PDT instrument of the utility model can observe the cooperation effect of fluorescence marking and photodynamic killing of tumor cells under different irradiation modes, analyze the immune mechanism of PDT treatment, determine the combination characteristics of photosensitizer-laser light irradiation mode for fluorescence marking and laser killing of tumor cells for photodynamic therapy, so that the photodynamic therapy is better, and good treatment effect is achieved.

[0025] 3) The diagnosis and treatment integrated PDT instrument of the utility model can provide a new instrument for patients, which is minimally invasive, can be used for a long time after operation, can be used multiple times, has the functions of monitoring tumor recurrence and treatment in one, improves the survival prognosis of patients, and can reduce diagnosis and treatment costs. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 is a structural schematic view of the utility model;

[0027] Figure 2 is a schematic view of the controller controlling the multi-wavelength control laser equipment irradiating the tissue in the utility model. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the utility model clearer and more apparent, the utility model will be further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the utility model and not to limit the utility model. In addition, the technical features involved in each embodiment of the utility model described below can be combined with each other as long as they do not conflict with each other.

[0029] The main factors affecting the clinical treatment effect of PDT are the selection and use of excitation light source, the selection and use of photosensitizer, treatment scheme and operation. In the PDT treatment process, the distribution of photosensitizer in the tissue, the light bleaching effect of photosensitizer, the propagation of light in the tissue, the distribution of oxygen in the tissue and the like affect the laser action of photodynamic power, and the absorption spectrum of photosensitizer is sometimes inconsistent with the action spectrum of photodynamic therapy, that is, the maximum absorption wavelength during laser is not necessarily the best treatment wavelength when killing tumor cells, therefore, the combination characteristics of photosensitizer-laser light irradiation mode need to be clear to achieve better photodynamic treatment effect.

[0030] To achieve the above object, the utility model provides a kind of diagnosis and treatment integration PDT instrument, including multi-wavelength control laser equipment 1 and imaging mechanism.

[0031] The multi-wavelength control laser equipment 1 includes fluorescence excitation laser device 2 for emitting laser to let photosensitizer on tissue 12 generate fluorescence and photodynamic therapy laser device 3 for emitting laser of multiple wavelengths to kill tumor cells on tissue 12;Fluorescence excitation laser device 2 and photodynamic therapy laser device 3 can all use conventional wavelength tunable laser, for emitting laser of multiple wavelengths.

[0032] The fluorescence excitation laser device 2 of the utility model preferably has multiple and they are all multimode laser, adopt multimode laser to illuminate larger area, and the wavelength of laser emitted by each fluorescence excitation laser device 2 is different.

[0033] For different photosensitizers, such as 5-ALA (Ai La, Zhangjiang Biology), Haiermopu (Fumeida, Zhangjiang Biology), configure multiple wavelengths and realize program-controlled switching (switching time window can be set) fluorescence excitation laser device 2, realize single wavelength output or multiple wavelength mixed output.

[0034] Each fluorescence excitation laser device 2 has a fiber interface A21.

[0035] The kind of diagnosis and treatment integration PDT instrument further includes fiber coupler A6, and the fiber coupler A6 has multiple laser entrances A and one laser exit A, and each laser entrance A is connected with one fiber interface A21.

[0036] The controller 10 is connected to the fluorescence excitation laser device 2 through a signal line, so that the fluorescence excitation laser device 2 emits laser of a set wavelength to excite photosensitizer on the tissue 12, thereby emitting fluorescence. The controller 10 is a built-in controller of the multi-wavelength regulated laser equipment 1, which is connected to an external controller 11.

[0037] The utility model discloses the photodynamic therapy laser device 3 has multiple and they are all single mode laser, and the wavelength of laser that each photodynamic therapy laser device 3 emits is different, and the aggregation of single mode laser is better, can kill tumor cells pertinently.

[0038] Each photodynamic therapy laser device 3 has an optical fiber interface B3 respectively;

[0039] The integrated diagnosis and treatment PDT instrument also includes a fiber coupler B7, the fiber coupler B7 has a plurality of laser entrances B and a laser exit B, and each laser entrance B is connected with an optical fiber interface B31 respectively.

[0040] The controller 10 is connected to the photodynamic therapy laser device 3 through a signal line, so that the photodynamic therapy laser device 3 emits laser of a set wavelength and a set emission mode, wherein the emission mode includes continuous light, long pulse light and short pulse light, the controller 10 is provided with a switch for controlling the opening and closing of the laser, and the controller 10 is also provided with a mode selection button for controlling the emission mode of the laser.

[0041] Referring to Figure 2 , the wavelength of the fluorescence excitation laser device 2 is selected as 405nm and 450nm. The wavelength of the photodynamic therapy laser device 3 is selected as 405nm, 514nm, 532nm and 630nm.

[0042] The imaging mechanism includes a camera 4 for obtaining color images and fluorescence images of the tissue 12, an image processing module and a display 5, the image processing module is connected with the camera 4, and the display 5 is connected with the image processing module.

[0043] The camera 4 includes a color CMOS image sensor, a black-and-white CMOS image sensor, a light splitting prism for separating white light and the fluorescence, and a filter set for transmitting fluorescence and filtering other wavelengths of visible light, the filter set is arranged between the black-and-white CMOS image sensor and the light splitting component. The filter set preferably has a plurality of filters, and the filters are switched to a set position by a programmed switching mode. The camera 4 preferably adopts a wide-spectrum camera.

[0044] The image processing module can superimpose the color image and the fluorescent image acquired by the camera 4 to generate a superimposed image. Through the superimposed image, both the color image around the lesion and the fluorescent image in which the lesion is marked by fluorescence can be clearly seen, so that the position of the lesion to be removed can be accurately determined, and small-volume lesions can be detected.

[0045] Further, the tumor diagnosis and treatment device 9 is further included, the tumor diagnosis and treatment device 9 includes the light dynamic irradiation catheter and implantation catheter that cooperate with each other, and the light dynamic interface on the light dynamic irradiation optical fiber built in the pipe body of the light dynamic irradiation catheter is connected with the laser outlet B.

[0046] Further, the test bench 8 for placing the tissue 12 is further included, and the ex vivo tissue 12 can be placed on the test bench 8 to carry out experiments.

[0047] Further, the fluorescence excitation laser device 2 and / or the photodynamic therapy laser device 3 meet the following conditions: output power is greater than or equal to 2W, the output pulse width is 1ms-999ms, the power instability is less than or equal to 5%, and the reproducibility is less than or equal to 5%.

[0048] The optical fiber interface A21 and / or the optical fiber interface B31 meet the following conditions: conforming to international standard SMA905 and the core diameter is greater than or equal to 400um.

[0049] The utility model discloses the instrument of adjustable different power multiband, can use different photosensitizer to handle tumor cell, observe tumor cell fluorescence marking and killing effect under different irradiation mode, determine the optimal combination of photosensitizer-laser light mode of realizing fluorescence marking and photodynamic killing treatment.

[0050] Those skilled in the art can easily understand that the above description is only a preferred embodiment of the utility model, and is not used to limit the utility model, and any modification, equivalent replacement and improvement made within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. A PDT device for diagnosis and treatment integration, comprising a multi-wavelength control laser device and an imaging mechanism, characterized in that, the multi-wavelength control laser device comprises a fluorescence excitation laser device for emitting laser to make photosensitizer on the tissue produce fluorescence and a photodynamic therapy laser device for emitting laser of multiple wavelengths to kill tumor cells on the tissue; the imaging mechanism comprises a camera for obtaining color images and fluorescence images of the tissue, an image processing module connected with the camera, and a display connected with the image processing module.

2. The diagnosis and treatment integrated PDT apparatus according to claim 1, wherein The fluorescence excitation laser device has multiple and they are all multimode lasers, and the wavelength of the laser emitted by each fluorescence excitation laser device is different.

3. The diagnosis and treatment integrated PDT apparatus according to claim 2, wherein Each fluorescence excitation laser device has a fiber interface A; The PDT device for diagnosis and treatment integration further comprises a fiber coupler A, which has multiple laser inlets A and one laser outlet A, and each laser inlet A is connected with one fiber interface A.

4. The diagnosis and treatment integrated PDT apparatus according to claim 1, wherein The controller is connected with the fluorescence excitation laser device through a signal line to make the fluorescence excitation laser device emit laser of a set wavelength.

5. The diagnosis and treatment integrated PDT apparatus according to claim 1, wherein The photodynamic therapy laser device has multiple and they are all single-mode lasers, and the wavelength of the laser emitted by each photodynamic therapy laser device is different.

6. The diagnosis and treatment integrated PDT apparatus according to claim 5, wherein Each photodynamic therapy laser device has a fiber interface B; The PDT device for diagnosis and treatment integration further comprises a fiber coupler B, which has multiple laser inlets B and one laser outlet B, and each laser inlet B is connected with one fiber interface B.

7. The diagnosis and treatment integrated PDT apparatus according to claim 6, wherein It further comprises a tumor diagnosis and treatment device, which comprises a photodynamic irradiation catheter and an implantation catheter used in cooperation, and the photodynamic interface on the photodynamic irradiation optical fiber built in the tube of the photodynamic irradiation catheter is connected with the laser outlet B.

8. The diagnosis and treatment integrated PDT apparatus according to claim 1, wherein The controller is connected with the photodynamic therapy laser device through a signal line to make the photodynamic therapy laser device emit laser of a set wavelength and a set emission mode, wherein the emission mode includes continuous light, long pulse light and short pulse light, the controller is installed with a switch for controlling the opening and closing of the laser, and the controller is also installed with a mode selection button for controlling the emission mode of the laser.

9. The diagnosis and treatment integrated PDT apparatus according to claim 1, wherein The fluorescence excitation laser device and / or the photodynamic therapy laser device meet the following conditions: output power ≥ 2W, output pulse width 1ms-999ms, power instability ≤ 5%, and reproducibility ≤ 5%; The fiber interface A and / or the fiber interface B meet the following conditions: conforming to international standard SMA905 and core diameter ≥ 400um.

10. The diagnosis and treatment integrated PDT apparatus according to claim 1, wherein It further comprises a test bench for placing the tissue.

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