Animal epidemic disease rapid detection all-in-one machine integrating nucleic acid extraction and amplification

Abstract

The utility model relates to the technical field of animal epidemic disease detection all-in-one machines, in particular to a nucleic acid extraction and amplification integrated animal epidemic disease rapid detection all-in-one machine which comprises a box body, a first partition plate is installed on the inner side of the box body, and a second partition plate is installed in the center of the top of the first partition plate. A fitting seat is mounted in the center of the top of the placement disc, lap joint grooves are formed in the two ends of the fitting seat, lap joint seats are slidably connected to the inner sides of the two lap joint grooves, a placement frame is mounted on the other side of the connecting plate, and the lap joint seats are connected with the fitting seat in a clamped mode through mounting mechanisms. The mounting mechanism comprises a butt joint assembly, a rotary extrusion assembly and an abutting assembly; the storage rack is simple in structure and convenient to operate, it can be guaranteed that the storage rack cannot slip or be dislocated, the operation can be completed only by buckling the pull ring and pressing and rotating the pull ring when the storage rack is disassembled and assembled, and the practical effect of the device is further improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to animal epidemic disease detection integrated machine technical field, concretely relates to nucleic acid extraction and amplification integrated animal epidemic disease rapid detection integrated machine. BACKGROUND

[0002] The method of animal nucleic acid extraction is divided into centrifugal column method and magnetic bead method, usually adopts the magnetic bead method to extract nucleic acid, then amplifies specific DNA fragments through PCR, that is, polymerase chain reaction, so that the amplified nucleic acid is compared, so as to achieve the purpose of detection, but at present, the process of nucleic acid extraction and fluorescent PCR detection is operated separately through different machines, which needs to be transported after nucleic acid extraction, which not only consumes long time, but also may cause pollution, thereby causing low work efficiency.

[0003] In order to solve the above technical problems, the prior art disclosed in Chinese patent No. CN219136786U provides a nucleic acid extraction and amplification integrated animal epidemic disease rapid detection integrated machine, a placing plate is installed in the middle of the box body, a rotating circular plate is rotatably installed at the top end of the placing plate, mounting plates are symmetrically and slidably connected to the two sides of the mounting frame, a rotating motor is installed at the middle of the bottom end of the placing plate through screws, a magnetic plate is connected to the bottom end of the electric push rod, magnetic rods are uniformly installed at the bottom end of the magnetic plate, a storage battery is installed at the bottom end of the box body, top baffles are symmetrically and rotatably installed at the top of the front of the box body.

[0004] Although the prior art scheme can slide to take out the mounting plate to install test tubes or replace and clean them, the mounting plate and the mounting frame do not have corresponding limiting, but only slide to butt joint, although the mounting plate can be freely pulled out, when the rotating motor drives the rotating circular plate to rotate and swing, the mounting plate is easy to slide out together with the test tubes, thereby causing the position deviation of the test tubes, the equipment inside the box body cannot be aligned with the test tubes to operate, and then the use effect of the device is affected. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a nucleic acid extraction and amplification integrated animal epidemic disease rapid detection integrated machine, to solve the problem that the mounting plate and the mounting frame do not have corresponding limiting, but only slide to butt joint, although the mounting plate can be freely pulled out, when the rotating motor drives the rotating circular plate to rotate and swing, the mounting plate is easy to slide out together with the test tubes, thereby causing the position deviation of the test tubes, the equipment inside the box body cannot be aligned with the test tubes to operate, and then the use effect of the device is affected.

[0006] To achieve the above object, the utility model provides the following technical scheme:

[0007] The utility model provides a nucleic acid extraction and amplification integrated animal epidemic disease rapid detection integrated machine, including the box, the inside of box is equipped with first baffle, the outer wall of box is located first baffle's both sides and is hinged with first open and close door and second open and close door respectively, the top central place of first baffle is equipped with second baffle, the top rotation of first baffle is connected with the placement tray, the top central place of placement tray is equipped with the fitment seat, the both ends of fitment seat are all set up the lap joint groove, two groups lap joint groove insides are all connected with lap joint seat slidingly, two groups lap joint seat one side is equipped with the connecting plate respectively, the other side of connecting plate is equipped with the placement frame, the top of placement frame is equipped with multiple groups of placement cover, lap joint seat is connected with fitment seat through the mounting mechanism, the mounting mechanism includes the docking assembly, the rotation extrusion subassembly and the abutment component, the docking assembly is used for the preliminary docking of lap joint seat and fitment seat, the rotation extrusion subassembly is used for the position of two groups lap joint seat and placement frame is unlocked with docking assembly, the abutment component is used for the position of two groups lap joint seat and placement frame is locked with rotation component.

[0008] As the preferred scheme of the utility model, the docking assembly includes a movable rod slidingly connected to the inside of the fitment seat, a rotating groove is formed in the outside of the movable rod inside the fitment seat, a rotating plate is slidingly connected to the inside of the rotating groove, the rotating plate is rotationally connected to the movable rod, first springs are installed between the both ends of one side of the rotating plate and the inner wall of the rotating groove, a retraction groove is formed in the outside of the movable rod outside the fitment seat, a clamping block is installed at one end of the movable rod, a docking groove is formed in one side of the lap joint seat, the docking groove is slidingly connected to the clamping block, and the cross section of the docking groove is in a cross shape.

[0009] As the preferred scheme of the utility model, the rotation extrusion assembly includes an installation groove formed in one side of the docking groove inside the lap joint seat, a bottom disc is rotationally connected to the inside of the installation groove, second springs are installed at the both ends of the top of the bottom disc, a docking disc is installed at the top end of the second springs, a positioning block is installed at the top of the docking disc, a clamping block is installed at the bottom end of the clamping block, and a positioning groove is formed in the clamping block to be connected to the positioning block.

[0010] As the preferred scheme of the utility model, the abutment component includes abutment grooves formed in both ends of the inner wall of the installation groove, third springs are installed in the inside of the abutment grooves, an abutment block is installed at one end of the third springs, the abutment block is slidingly connected to the abutment groove, fixed blocks are installed at the both ends of the top of the clamping block, a fixed groove is formed in the bottom end of the abutment block to be connected to the fixed blocks, and an extension groove is formed in one side of the rotating groove at the top of the fitment seat.

[0011] As the preferred scheme of the utility model, the top of the fitting seat is hinged with a protective plate at both ends, one side of the protective plate is provided with a buckle slot, one end of the protective plate near the extension slot is embedded with a first fixing part, and the inner wall of the extension slot is embedded with a second fixing part corresponding to the first fixing part.

[0012] As the preferred scheme of the utility model, the top of the fitting seat is hinged with a protective plate at both ends, one side of the protective plate is provided with a buckle slot, one end of the protective plate near the extension slot is embedded with a first fixing part, and the inner wall of the extension slot is embedded with a second fixing part corresponding to the first fixing part.

[0013] As the preferred scheme of the utility model, the top of the fitting seat is hinged with a protective plate at both ends, one side of the protective plate is provided with a buckle slot, one end of the protective plate near the extension slot is embedded with a first fixing part, and the inner wall of the extension slot is embedded with a second fixing part corresponding to the first fixing part.

[0014] Compared with the prior art, the utility model has the beneficial effects that:

[0015] In the utility model, the butt joint assembly is used for initially butt jointing the lap joint seat and the fitting seat, the rotating extrusion assembly is used for unlocking the positions of the two sets of lap joint seats and the placing rack in cooperation with the butt joint assembly, the abutting assembly is used for locking the positions of the two sets of lap joint seats and the placing rack in cooperation with the rotating assembly, the structure is simple and convenient to operate, the placing rack can be prevented from slipping and misplacing, and when the placing rack is disassembled and assembled, the operation can be completed by only buckling and pressing the pull ring, and the practical effect of the device is further increased. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the whole structure schematic view of the utility model;

[0017] Figure 2 It is the structure schematic view without the first opening and closing door and the second opening and closing door of the utility model;

[0018] Figure 3 It is the local three-dimensional structure schematic view of the placing disc and the fitting seat of the utility model;

[0019] Figure 4 It is the local sectional view structure schematic view of the fitting seat and the lap joint seat of the utility model;

[0020] Figure 5 It is the local three-dimensional structure schematic view of the fitting seat of the utility model.

[0021] In the figure: 1, box; 2, first partition; 3, second partition; 4, placing disc; 5, fitting seat; 6, lapping seat; 7, connecting plate; 8, placing rack; 9, rotating plate; 10, butt joint disc; 11, positioning block; 12, abutting groove; 13, abutting block; 14, fixed block; 15, butt joint groove; 16, protection plate; 17, first fixing part; 18, pull ring; 19, rotating motor; 20, bottom disc; 21, clamping block; 22, placing sleeve. DETAILED DESCRIPTION

[0022] The technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0023] Embodiment:

[0024] Please refer to Figures 1-5 The utility model provides a technical scheme:

[0025] A nucleic acid extraction and amplification integrated animal epidemic disease rapid detection all -in -one, including box 1, its characterized in that: the inside of box 1 is installed with first partition 2, and the outer wall of box 1 is hinged with first opening and closing door and second opening and closing door respectively at the both sides of first partition 2, the top central portion of first partition 2 is installed with second partition 3, and the top of first partition 2 is rotatably connected with placing disc 4, the top central portion of placing disc 4 is installed with fitting seat 5, and the both ends of fitting seat 5 are provided with lapping groove, and the inside of two groups of lapping grooves is slidably connected with lapping seat 6, and the side of two groups of lapping seats 6 is installed with connecting plate 7 respectively, and the other side of connecting plate 7 is installed with placing rack 8, and the top end of placing rack 8 is installed with multiple groups of placing sleeve 22, and lapping seat 6 is clamped with fitting seat 5 through mounting mechanism, and the mounting mechanism includes butt joint assembly, rotating extrusion assembly and abutting assembly, butt joint assembly is used to preliminarily butt joint lapping seat 6 and fitting seat 5, rotating extrusion assembly is used to unlock the position of two groups of lapping seats 6 and placing rack 8 in cooperation with butt joint assembly, and abutting assembly is used to lock the position of two groups of lapping seats 6 and placing rack 8 in cooperation with rotating assembly, when using the device, can preliminarily butt joint lapping seat 6 and fitting seat 5 through butt joint assembly, unlock the position of two groups of lapping seats 6 and placing rack 8 in cooperation with rotating extrusion assembly and butt joint assembly, and lock the position of two groups of lapping seats 6 and placing rack 8 in cooperation with abutting assembly and rotating assembly, simple structure, convenient operation can guarantee that placing rack 8 will not appear to slip and misposition, and when disassembling placing rack 8, only need to buckle pull ring 18 and press and turn to complete the operation, further increase the practical effect of the device.

[0026] In the embodiment, as Figure 1、 Figure 2 and Figure 3 As shown in , the docking assembly comprises a movable rod slidingly connected to the inside of the fitting seat 5, a rotating groove is formed on the outside of the movable rod, a rotating plate 9 is slidingly connected to the inside of the rotating groove, the rotating plate 9 is rotationally connected to the movable rod, first springs are installed between the two ends of one side of the rotating plate 9 and the inner wall of the rotating groove, a retracting groove is formed on the outside of the movable rod, a clamping block 21 is installed at one end of the movable rod, a docking groove 15 is formed on one side of the lapping seat 6, the docking groove 15 is slidingly connected to the clamping block 21, the cross section of the docking groove 15 is cross-shaped, and the cross section of the mounting groove is circular. First, the connecting plate 7 is attached to one side of the fitting seat 5, the lapping seat 6 is placed inside the lapping groove, the cross section of the docking groove 15 is cross-shaped, the cross section of the mounting groove is circular, the movable rod is pressed to drive the clamping block 21 to move downward, the clamping block 21 slides into the horizontal opening of the docking groove 15, and the rotating plate 9 is stretched to stretch the first spring.

[0027] Figure 2 , Figure 3 and Figure 4 As shown in , the rotating extrusion assembly comprises a mounting groove formed on one side of the docking groove 15 inside the lapping seat 6, a bottom disc 20 is rotationally connected to the inside of the mounting groove, second springs are installed at the top of both ends of the bottom disc 20, a docking disc 10 is installed at the top end of the second spring, a positioning block 11 is installed at the top of the docking disc 10, a positioning groove is formed at the bottom end of the clamping block 21 and is clamped with the positioning block 11, then the two ends of the clamping block 21 contact the abutting blocks 13 on both sides, the inclined surfaces of the two abutting blocks 13 contact each other, the abutting blocks 13 are extruded to drive the third spring to retract, after the clamping block 21 and the fixed block 14 installed thereon continue to move downward and are offset from the abutting blocks 13, the abutting blocks 13 rebound, at this time the positioning block 11 is clamped into the positioning groove, the docking disc 10 compresses the second spring to retract, then it is pulled back for a distance, the fixed block 14 is clamped into the inside of the fixed groove, the abutting blocks 13 and the docking disc 10 tightly abut the clamping block 21, and the locking of the positions of the lapping seat 6 and the placement rack 8 is completed.

[0028] Figure 2 , Figure 3 and Figure 4As shown, the abutting assembly includes abutting grooves 12 opened at both ends of the inner wall of the mounting groove, the third spring is mounted inside the abutting groove 12, one end of the third spring is provided with an abutting block 13, the abutting block 13 is in sliding connection with the abutting groove 12, the top of the clamping block 21 is provided with a fixed block 14 at both ends, the bottom end of the abutting block 13 is provided with a fixed groove for clamping the fixed block 14, the top of the fitting seat 5 is provided with an extension groove on one side of the rotating groove, the top of the fitting seat 5 is hingedly provided with a protective plate 16 at both ends, one side of the protective plate 16 is provided with a buckle slot, one end of the protective plate 16 close to the extension groove is embeddedly provided with a first fixing member 17, the inner wall of the extension groove is embeddedly provided with a second fixing member corresponding to the first fixing member 17, further, when it is needed to unlock the placement rack 8 for cleaning, the movable rod is squeezed again to cooperate with the positioning block 11 and the positioning groove to make the abutting block 13 and the first spring move downward, then the bottom disc 20 is rotated, the abutting block 13 is shifted, the upper positioning plate 4 is held and pulled upward, the clamping block 21 is slid out of the longitudinal opening of the abutting groove 15, and is retracted into the retraction groove for resetting, at this time, the lapping seat 6 can be taken out of the fitting seat 5.

[0029] In this embodiment, as shown in Figure 3 、 Figure 4 and Figure 5 , one end of the movable rod extending to the inside of the extension groove is rotatably connected with a pull ring 18, one of the first fixing member 17 and the second fixing member is a first magnet, the other of the first fixing member 17 and the second fixing member is a second magnet, the magnetic poles of the first magnet and the second magnet are opposite and attract each other, the inside of the box body 1 is mounted with a rotating motor 19 below the first partition plate 2, the driving end of the rotating motor 19 extends to the inside of the first partition plate 2 and is fixedly connected with one side of the placement disc 4, the inside of the box body 1 is mounted with a storage battery on one side of the rotating motor 19, further, after the fitting seat 5 and the lapping seat 6 are locked, the buckle slot on one side of the protective plate 16 is buckled, the protective plate 16 and the inside of the extension groove are fitted, the first fixing member 17 and the second fixing member cover the pull ring 18 inside the extension groove, so as to prevent the pull ring 18 from being touched by mistake after installation, causing the connection of the parts to be loose.

[0030] The implementation principle of the nucleic acid extraction and amplification integrated animal epidemic rapid detection integrated machine is as follows: the connecting plate 7 is attached to one side of the attaching seat 5, the lapping seat 6 is arranged in the lapping groove, the cross section of the butt joint groove 15 is cross-shaped, the cross section of the mounting groove is circular, the movable rod is pressed to drive the clamping block 21 to move downward, the clamping block 21 slides into the transverse opening of the butt joint groove 15, the rotating plate 9 is stretched to drive the first spring, the two ends of the clamping block 21 contact the abutting blocks 13 on the two sides, the inclined surfaces of the two abutting blocks 13 contact each other, the abutting blocks 13 are pressed to drive the third spring to retract, after the clamping block 21 and the fixed block 14 arranged thereon continue to move downward and are disengaged from the abutting blocks 13, the abutting blocks 13 rebound, the positioning block 11 is clamped into the positioning groove, the butt joint disc 10 presses the second spring to retract, then the fixed block 14 is clamped into the fixed groove, the abutting blocks 13 and the butt joint disc 10 abut the clamping block 21, the position locking of the lapping seat 6 and the placing rack 8 is completed, when the locking of the placing rack 8 needs to be unlocked for cleaning, the movable rod is pressed again to cooperate with the positioning block 11 and the positioning groove to make the butt joint disc 10 and the first spring move downward, the bottom plate 20 is further rotated to disengage the abutting blocks 13, the clamping block 21 is pulled out of the longitudinal opening of the butt joint groove 15, and is retracted into the retraction groove to reset, then the lapping seat 6 can be taken out of the attaching seat 5, after the attaching seat 5 and the lapping seat 6 are locked, one side of the protection plate 16 is clamped in the buckle slot, the protection plate 16 is attached to the extension groove, the first fixing part 17 and the second fixing part are arranged in the extension groove, and the pull ring 18 is arranged in the extension groove, so that the pull ring 18 is prevented from being touched by mistake after installation, and the connection of the parts is prevented from being loosened.

[0031] The control mode of the utility model is controlled through the controller, the control circuit of the controller can be realized through simple programming of the person skilled in the art, the power supply also belongs to the public knowledge in the field, and the utility model is used to protect the mechanical device, so the control mode and the circuit connection of the utility model will not be explained in detail.

[0032] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and modifications can be made to the embodiments without departing from the principles and spirits of the utility model, the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid extraction and amplification integrated animal disease rapid detection integrated machine, comprising a box (1), characterized in that: The inner side of the box (1) is provided with a first partition (2), and the outer wall of the box (1) is hinged with a first opening and closing door and a second opening and closing door on the two sides of the first partition (2), respectively. The top center of the first partition (2) is provided with a second partition (3), and the top of the first partition (2) is rotatably connected with a placing disc (4). The top center of the placing disc (4) is provided with a fitting seat (5), and the both ends of the fitting seat (5) are provided with overlapping grooves. The inner sides of the two groups of overlapping grooves are slidably connected with overlapping seats (6). The two groups of overlapping seats (6) are respectively provided with connecting plates (7) on one side. The other side of the connecting plate (7) is provided with a placing rack (8). The top end of the placing rack (8) is provided with a plurality of placing sleeves (22). The overlapping seat (6) is clamped with the fitting seat (5) through the mounting mechanism. The mounting mechanism includes a docking assembly, a rotating extrusion assembly and an abutting assembly. The docking assembly is used for preliminary docking of the overlapping seat (6) and the fitting seat (5). The rotating extrusion assembly is used for unlocking the position of the two groups of overlapping seats (6) and the placing rack (8) in cooperation with the docking assembly. The abutting assembly is used for locking the position of the two groups of overlapping seats (6) and the placing rack (8) in cooperation with the rotating assembly.

2. The nucleic acid extraction and amplification integrated animal disease rapid detection integrated machine according to claim 1, characterized in that: The docking assembly includes a movable rod slidably connected in the fitting seat (5). A rotating groove is formed in the outer side of the movable rod in the fitting seat (5). A rotating plate (9) is slidably connected in the rotating groove. The rotating plate (9) is rotatably connected with the movable rod. First springs are arranged between the both ends of one side of the rotating plate (9) and the inner wall of the rotating groove. A retraction groove is formed in the outer side of the movable rod outside the fitting seat (5). A clamping block (21) is arranged at one end of the movable rod. An abutting groove (15) is formed in one side of the overlapping seat (6). The abutting groove (15) is slidably connected with the clamping block (21). The cross section of the abutting groove (15) is in the shape of a cross.

3. The nucleic acid extraction and amplification integrated animal disease rapid detection integrated machine according to claim 2, characterized in that: The rotating extrusion assembly includes an installation groove formed in the inner side of the overlapping seat (6) on one side of the abutting groove (15). A bottom disc (20) is rotatably connected in the installation groove. Second springs are arranged at the both ends of the top of the bottom disc (20). An abutting disc (10) is arranged at the top end of the second spring. A positioning block (11) is arranged at the top of the abutting disc (10). A clamping block (21) is arranged at the bottom end of the abutting disc (10). A positioning groove is formed in the clamping block (21) and is clamped with the positioning block (11).

4. The nucleic acid extraction and amplification integrated animal disease rapid detection integrated machine according to claim 3, characterized in that: The abutting assembly includes abutting grooves (12) formed in the both ends of the installation groove. Third springs are arranged in the inner sides of the abutting grooves (12). Abutting blocks (13) are arranged at one end of the third springs. The abutting blocks (13) are slidably connected with the abutting grooves (12). Fixed blocks (14) are arranged at the both ends of the top of the clamping block (21). Fixed grooves are formed in the bottom end of the abutting block (13) and are clamped with the fixed blocks (14). An extension groove is formed in one side of the rotating groove at the top of the fitting seat (5).

5. The nucleic acid extraction and amplification integrated animal disease rapid detection integrated machine according to claim 4, characterized in that: The top of the fitting seat (5) is hinged with a protective plate (16) at both ends, one side of the protective plate (16) is provided with a buckle slot, one end of the protective plate (16) close to the extension slot is embedded with a first fixing part (17), the inner wall of the extension slot is embedded with a second fixing part corresponding to the first fixing part (17).

6. The nucleic acid extraction and amplification integrated animal disease rapid detection integrated machine according to claim 5, characterized in that: One end of the movable rod extending to the inside of the extension slot is rotatably connected with a pull ring (18), one of the first fixing part (17) and the second fixing part is a first magnet, the other of the first fixing part (17) and the second fixing part is a second magnet, the magnetic poles of the first magnet and the second magnet are opposite and attract each other.

7. The nucleic acid extraction and amplification integrated animal disease rapid detection integrated machine according to claim 6, characterized in that: The inside of the box (1) below the first partition plate (2) is provided with a rotary motor (19), the driving end of the rotary motor (19) extends to the inside of the first partition plate (2) and is fixedly connected with one side of the placing disc (4), the inside of the box (1) on one side of the rotary motor (19) is provided with a storage battery.

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