Stacked cell culture dish

Abstract

The utility model relates to the technical field of culture dishes, in particular to a stacked cell culture dish which comprises a box body, the top of the box body is sleeved and screwed with a cover body, a limiting ring is fixed on the top surface of the cover body, a limiting groove is formed in the bottom surface of the box body, and after being stacked, two groups of cell culture dishes are sleeved and limited through the limiting ring and a shifting piece; the outer side of the box body is sleeved with a baffle ring, a plurality of second air holes are formed in the surface of the baffle ring, and a plurality of first air holes are formed in the surface of the box body; the packaging box has the beneficial effects that the box body and the cover body are in threaded connection to form a sealing structure, and the limiting ring and the limiting groove which are used for stacking and limiting are arranged. When a large number of cells need to be cultured or multiple groups of experiments need to be carried out, a plurality of culture dishes can be stacked, so that the experiment space is greatly saved, and the space utilization rate is increased.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of culture dish, concretely to a superposed cell culture dish. BACKGROUND

[0002] In the field of cell culture, cell culture dishes are important tools for culturing cells in vitro. Traditional cell culture dishes are usually single-layer structures. When a large number of cells need to be cultured or multiple experiments need to be performed, a large amount of experimental space is occupied, which is not conducive to efficient use of space. Moreover, when cell culture experiments under aerobic and anaerobic conditions need to be performed simultaneously, two different sets of culture equipment or complex modifications to the same set of equipment are often required, which is cumbersome to operate and increases the experimental cost.

[0003] In addition, during cell culture, the stability of the stacked culture dishes is also a key issue. If the stacking is unstable, the culture dishes are prone to tipping over, causing the cell culture solution to spill and the cells to be contaminated, which affects the experimental results. At the same time, it is difficult to regulate the sealing and air permeability of the culture dishes, and it is difficult to accurately control the oxygen content in the culture environment according to the experimental requirements.

[0004] Therefore, it is of great practical significance to develop a cell culture dish that has good stacking stability, can easily adjust aerobic and anaerobic culture conditions, and saves space. SUMMARY

[0005] The utility model aims at providing a superposed cell culture dish to solve the problems raised in the background.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a superposed cell culture dish, comprising a box body, a lid body is sleeved with a screw on the top of the box body, a limiting ring is fixed on the top surface of the lid body, a limiting groove is opened on the bottom surface of the box body, and the two groups of cell culture dishes are stacked and limited by the limiting ring and the push piece; a first stop ring is sleeved on the outside of the box body, a plurality of air holes two are opened on the surface of the first stop ring, and a plurality of air holes one are opened on the surface of the box body.

[0007] Preferably, the limiting ring is in a circular ring structure, the limiting groove is in a circular groove, a rubber ring is fixed on the side wall of the limiting groove, and the inner diameter of the rubber ring is smaller than the outer diameter of the limiting ring.

[0008] Preferably, the top end of the box body is provided with an external thread on the outer ring surface, the inner wall of the lid body is provided with an internal thread, and the internal thread and the external thread are connected in cooperation.

[0009] Preferably, the inner wall of the limiting ring is fixed with a push piece, the push piece is in a "cross" plate structure, and the height of the push piece is equal to the height of the limiting ring.

[0010] Preferably, the first blocking ring is a circular ring structure with a cross-section of "convex" shape, and the top of the first blocking ring and the bottom of the first blocking ring are each provided with a second blocking ring, and the second blocking ring is sleeved and fixed on the outer wall of the box body.

[0011] Preferably, the inner wall of the first blocking ring is fixed with a rubber clamp strip, and a plurality of rubber clamp strips are arranged, and the rubber clamp strip and the second gas permeable hole are distributed.

[0012] Compared with the prior art, the utility model has the beneficial effects that:

[0013] The stacked cell culture dish disclosed by the utility model adopts a sealing structure of screwing a box body and a cover body, and is provided with a limiting ring and a limiting groove for limiting stacking. When a large number of cells need to be cultured or a plurality of experiments need to be carried out, a plurality of culture dishes can be stacked and placed, so that the experimental space is greatly saved, and the space utilization is improved.

[0014] The outer side of the box body is sleeved with a blocking ring, a plurality of second gas permeable holes are formed in the surface of the blocking ring, and a plurality of first gas permeable holes are formed in the surface of the box body. When the blocking ring is rotated to correspond to the second gas permeable hole and the first gas permeable hole, aerobic culture is realized in the box body; after the blocking ring is rotated around the box body to be out of position with the second gas permeable hole, the rubber clamp strip blocks the second gas permeable hole, and anaerobic culture is realized in the box body. This design makes the adjustment of aerobic culture and anaerobic culture very flexible, and the oxygen content in the culture environment can be quickly and accurately adjusted according to the experimental requirements. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a structural schematic view of the utility model;

[0016] Figure 2 It is a structural plan view of the utility model;

[0017] Figure 3 It is a structural plan view of the utility model; Figure 2 It is a structural plan view of the utility model;

[0018] Figure 4 It is a structural schematic view of the utility model;

[0019] Figure 5 It is a structural schematic view of the utility model;

[0020] Figure 6 It is a structural plan view of the utility model; Figure 5 It is a structural plan view of the utility model;

[0021] Figure 7 It is a structural plan view of the utility model; Figure 6 It is a structural plan view of the utility model;

[0022] Figure 8 It is a structural plan view of the utility model; Figure 7 It is a structural plan view of the utility model; It is a structural plan view of the utility model;

[0023] In the figure: box body 1, cover body 2, limiting ring 3, push piece 4, limiting groove 5, air hole one 6, first blocking ring 7, air hole two 8, second blocking ring 9, rubber clamping strip 10, rubber ring 11. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical scheme of the utility model to be clear, complete description, and the advantage is more clear and apparent, the following will combine the figure to the utility model embodiment to carry out further detailed explanation. It should be understood that the specific embodiments described here are part of the embodiments of the utility model, not all embodiments, only to explain the utility model embodiments, and not for limiting the utility model embodiments, all other embodiments obtained by the ordinary skill in the art without doing creative work, belong to the scope of the utility model protection.

[0025] Please refer to Figures 1-8 , the utility model provides a technical scheme: a superimposed cell culture dish, including box body 1, the top of box body 1 is sleeved with cover body 2, the top surface of cover body 2 is fixed with limiting ring 3, the bottom surface of box body 1 is provided with limiting groove 5, after two groups of cell culture dishes are stacked, limiting ring 3 and push piece 4 are sleeved to limit; limiting ring 3 is circular ring structure, limiting groove 5 is circular groove, the side wall of limiting groove 5 is fixed with rubber ring 11, the inner diameter of rubber ring 11 is less than the outer diameter of limiting ring 3; the top end of box body 1 is provided with external thread on the outer ring surface, the inner wall of cover body 2 is provided with internal thread, and the internal thread and the external thread are connected in a matched mode; the inner wall of limiting ring 3 is fixed with push piece 4, push piece 4 is "cross" plate structure, and the height of push piece 4 is equal to the height of limiting ring 3. When using, push piece 4 is pinched by hand to cover cover body 2 on the top of box body 1, then push piece 4 is twisted to drive cover body 2 to be screwed on the top of box body 1, and the top of box body 1 is realized to be covered; when two groups of box body 1 provided with cover body 2 are stacked, the limiting ring 3 on the top of the bottom group of box body 1 is inserted into the limiting groove 5 at the bottom end of the bottom group of box body 1, and the two groups of stacked box body 1 are prevented from being inclined and limited.

[0026] The outer side of the box body 1 is sleeved with a first blocking ring 7, the surface of the first blocking ring 7 is provided with a plurality of air holes two 8, and the surface of the box body 1 is provided with a plurality of air holes one 6; the first blocking ring 7 is a circular ring structure with a "convex" cross section, the top of the first blocking ring 7 and the bottom of the first blocking ring 7 are both provided with a second blocking ring 9, and the second blocking ring 9 is sleeved and fixed on the outer wall of the box body 1; the inner wall of the first blocking ring 7 is fixed with a plurality of rubber clamping strips 10, the rubber clamping strips 10 are arranged alternately with the air holes two 8. In use, when the first blocking ring 7 is rotated to correspond to the air holes two 8 and the air holes one 6, aerobic culture is realized in the box body 1, after the first blocking ring 7 is rotated around the box body 1 to be dislocated from the air holes two 8, the rubber clamping strips 10 block the air holes two 8, at this time, anaerobic culture is realized in the box body 1, the aerobic and anaerobic culture can be adjusted by rotating the first blocking ring 7, and the second blocking ring 9 is clamped between the first blocking ring 7 and the box body 1, the friction between the first blocking ring 7 and the box body 1 is increased, and the rotation of the first blocking ring 7 without external force is avoided.

[0027] The use method of the superimposed cell culture dish is as follows: the cover body is aligned with the top of the box body, and is slowly lowered to preliminarily cover the box body. The knob is screwed to drive the cover body to be connected with the box body along the outer thread at the top end of the box body and the inner thread on the inner wall of the cover body, until the cover body is completely screwed on the top of the box body, so that the top of the box body is sealed. When two groups of box bodies provided with cover bodies need to be stacked, the limiting ring on the top of the bottom group of box bodies is aligned with the limiting groove at the bottom end of the upper group of box bodies. The upper box body is slowly lowered, and the limiting ring on the top of the bottom box body is inserted into the limiting groove at the bottom end of the upper box body. Since the limiting groove is fixed with a rubber ring, and the inner diameter of the rubber ring is smaller than the outer diameter of the limiting ring, the rubber ring will be elastically deformed during the insertion process, and the rubber ring will recover the deformation after the insertion, so that the two groups of box bodies stacked are prevented from being deviated and positioned. The initial position of the blocking ring is checked to confirm whether the air holes two on the blocking ring correspond to the air holes one on the surface of the box body. If not, adjustment is needed. The blocking ring is rotated around the box body until the air holes two on the blocking ring completely correspond to the air holes one on the surface of the box body. At this time, the box body is in communication with the outside air through the air holes one and the air holes two, and aerobic culture is realized. The blocking ring is rotated around the box body until the blocking ring completely blocks the air holes two. At this time, the rubber clamping strip on the inner wall of the blocking ring blocks the air holes two, preventing the outside air from entering the box body, and anaerobic culture is realized. After the position of the blocking ring is adjusted, the blocking ring clamped between the blocking ring and the box body at the top and the bottom increases the friction between the blocking ring and the box body, so that the rotation of the blocking ring without external force is avoided, and the stability of the culture environment is ensured.

[0028] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A stacked cell culture dish, comprising a box body (1), a cover body (2) is sleeved with the box body (1) by screwing, characterized in that: The top surface of the cover (2) is fixed with a limiting ring (3), the bottom surface of the box body (1) is provided with a limiting groove (5), after two groups of cell culture dishes are stacked, the limiting ring (3) and the push piece (4) are sleeved to limit; the outer side of the box body (1) is sleeved with a first blocking ring (7), the surface of the first blocking ring (7) is provided with a plurality of air holes two (8), and the surface of the box body (1) is provided with a plurality of air holes one (6).

2. The stacked cell culture dish of claim 1, wherein: The limiting ring (3) is in a circular ring structure, the limiting groove (5) is in a circular groove, the side wall of the limiting groove (5) is fixed with a rubber ring (11), and the inner diameter of the rubber ring (11) is smaller than the outer diameter of the limiting ring (3).

3. The stacked cell culture dish of claim 1, wherein: The top end of the box body (1) is provided with external threads on the outer ring surface, and the inner wall of the cover (2) is provided with internal threads; the internal threads and the external threads are connected in a matched mode.

4. The stacked cell culture dish of claim 1, wherein: The inner wall of the limiting ring (3) is fixed with the push piece (4), the push piece (4) is in a "cross" plate-shaped structure, and the height of the push piece (4) is equal to the height of the limiting ring (3).

5. The stacked cell culture dish of claim 1, wherein: The first blocking ring (7) is in a circular ring structure with a "convex" cross section, the top of the first blocking ring (7) and the bottom of the first blocking ring (7) are both provided with a second blocking ring (9), and the second blocking ring (9) is sleeved and fixed on the outer wall of the box body (1).

6. The stacked cell culture dish of claim 1, wherein: The inner wall of the first blocking ring (7) is fixed with a rubber clamping strip (10), a plurality of rubber clamping strips (10) are arranged, and the rubber clamping strips (10) and the air holes two (8) are arranged alternately.

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