Density gradient centrifugation sample separation and collection device

Abstract

The utility model discloses a density gradient centrifugal sample separation and collection device which comprises a body, a separation analysis part, a recovery part and a main control part, wherein a sample groove is formed in the body; the separation analysis part is arranged at the top of the body, a sample channel is formed in the separation analysis part, and the inlet end of the sample channel corresponds to the sample groove; the recovery part is arranged at the top of the body and corresponds to the outlet end of the sample channel; the main control part is arranged on the body, controls the sample groove to move towards the separation analysis part so as to enable the density gradient centrifugal samples of different layers to sequentially enter the sample channel for separation, and controls the recovery part to respectively receive the separated density gradient centrifugal samples. The device is controlled by the main control part to work, so that a density gradient centrifugal sample is quickly separated after passing through the separation analysis part, the operation is simple, the flow time is short, the sample separation accuracy can be ensured to the greatest extent, the pollution in the whole separation process is reduced, and the separation and storage of a biological sample are facilitated.

Description

TECHNICAL FIELD

[0001] The utility model relates to the separation collection technical field of centrifugal product, especially a density gradient centrifugal sample separation collection device. BACKGROUND

[0002] Ultracentrifugation technology is the most commonly used technology for separating and purifying biological macromolecules. When different particles with small centrifugal differences are separated, density gradient centrifugation technology is often relied on. Density gradient centrifugation technology can separate samples with similar sedimentation coefficients. The samples are separated by centrifugal force in the density gradient medium and finally distributed to a specific position in the gradient medium. Density gradient centrifugation technology is a commonly used method for biological molecule separation, widely used in fields such as virus separation and purification, protein separation, etc. Generally, it includes three steps: first, prepare the density gradient medium in the centrifuge tube, and place the sample to be separated above the medium; use the ultracentrifuge to centrifuge, so that each component in the sample to be separated is layered in the centrifuge tube; finally, collect and detect the sample distribution in each gradient in the medium after centrifugation. Therefore, the key of the density gradient method is how to take out the layered sample after centrifugation layer by layer without disturbing the upper and lower layers of the gradient medium, so as to ensure the purity of the separated sample in each layer.

[0003] At present, for the collection and detection of the final sample of the density gradient method, a pipette or a syringe is used to extract the sample after centrifugation by volume, and then the sample is analyzed. However, this method has a large disturbance to the sample, and there is blindness in the collection of the sample, which is time-consuming and requires high human operation, and it is difficult to meet the user's demand for rapid separation of the sample. Therefore, the full-automatic density gradient separation system is used in the prior art to realize the full-automatic separation, detection and collection of the sample. However, this kind of full-automatic density gradient separation system still has the problems of complicated operation, high operation environment requirement and being not conducive to the preservation of biological samples.

[0004] Therefore, the prior art still needs to be improved and developed. UTILITY MODEL CONTENT

[0005] In view of the above problems of the prior art, the utility model aims to provide a density gradient centrifugal sample separation and collection device, which aims to solve the problems of complicated operation, tedious steps and high operation environment requirement in the separation process of the density gradient centrifugal sample separation in the prior art, and is not conducive to the separation and preservation of biological samples.

[0006] The utility model provides a density gradient centrifugal sample separation and collection device, which comprises:

[0007] A body is provided with a sample groove inside to place the density gradient centrifugal sample;

[0008] A separation and analysis part is arranged on the top of the body, and a sample channel is formed inside the separation and analysis part, and an inlet end of the sample channel is arranged corresponding to the sample groove to receive the density gradient centrifugation sample;

[0009] A recovery part is arranged on the top of the body and arranged corresponding to an outlet end of the sample channel to receive the density gradient centrifugation sample passing through the sample channel;

[0010] A main control part is arranged on the body to control the sample groove to move towards the separation and analysis part to make the density gradient centrifugation sample of different layers enter the sample channel in sequence for separation, and to control the recovery part to receive the separated density gradient centrifugation sample respectively.

[0011] In an embodiment, the sample groove comprises:

[0012] A placement position is arranged at a central position of the sample groove to place a centrifuge tube containing the density gradient centrifugation sample, and an opening is arranged on the top of the body corresponding to the placement position;

[0013] A first motor is arranged below the placement position to push the placement position to move in a vertical direction under the control of the main control part, so that the centrifuge tube in the placement position enters the separation and analysis part through the opening, and the density gradient centrifugation sample of different layers in the centrifuge tube is sequentially extruded into the sample channel.

[0014] In an embodiment, in the separation and analysis part, the inlet end of the sample channel is in an inverted trumpet shape, and a gap is formed between the inlet end and the side wall of the separation and analysis part, so that after the centrifuge tube enters the separation and analysis part, the tube wall of the centrifuge tube is located between the inlet end and the side wall and is sleeved on the inlet end, and the density gradient centrifugation sample of different layers in the centrifuge tube is sequentially extruded into the sample channel.

[0015] In an embodiment, the separation and analysis part further comprises:

[0016] A sealing ring is arranged outside the inlet end of the sample channel to seal the centrifuge tube and the inlet end of the sample channel after the centrifuge tube is sleeved on the inlet end, so as to ensure that the density gradient centrifugation sample enters the sample channel in a layered order;

[0017] An analysis detector is arranged on both sides of the sample channel to obtain data of the density gradient centrifugation sample passing through the sample channel, so as to determine the time of the density gradient centrifugation sample of different layers passing through the sample channel, and to realize the separation of the density gradient centrifugation sample.

[0018] In an embodiment, the recovery part comprises:

[0019] A test tube rack, wherein a plurality of test tube positions are provided on the test tube rack for placing a plurality of test tubes;

[0020] A second motor, wherein the second motor is arranged below the test tube rack to control the movement of the test tube rack so that the plurality of test tubes are sequentially aligned with the outlet end of the sample channel to receive the separated density gradient centrifuged sample respectively.

[0021] In an embodiment, the test tube rack is in a disc structure, and the second motor controls the rotation of the test tube rack so that the plurality of test tubes are sequentially aligned with the outlet end of the sample channel.

[0022] In an embodiment, the main control part comprises:

[0023] A main control panel, wherein the main control panel is arranged on the side of the body to receive the data of the density gradient centrifuged sample obtained from the analysis detector and generate a waveform diagram to determine the composition of the density gradient centrifuged sample currently flowing through the sample channel;

[0024] A main control button, wherein the main control button is arranged below the main control panel to control the operation of the density gradient centrifuged sample separation device and set the corresponding operation parameters.

[0025] In an embodiment, further comprising:

[0026] A fixing support, wherein the fixing support is fixedly arranged on the top of the body to hold the separation and analysis part so that the inlet end of the sample channel is fixed above the opening.

[0027] In an embodiment, further comprising:

[0028] An ultrasonic cleaning part, wherein the ultrasonic cleaning part is arranged on the top of the body to clean the separation and analysis part after the separation and analysis part is removed from the fixing support;

[0029] A temperature control part, wherein the temperature control part is arranged around the body to control the temperature of the density gradient centrifuged sample in the density gradient centrifuged sample separation and collection device.

[0030] In an embodiment, the side of the body is provided with a sliding door to place the centrifuge tube containing the density gradient centrifuged sample into the sample slot.

[0031] In summary, the utility model discloses a density gradient centrifugal sample separation and collection device, comprising: the body, separation analysis part, recovery part and main control unit. In which, the body inside is equipped with sample groove to place density gradient centrifugal sample, separation analysis part sets up at the top of body, the inside of separation analysis part forms sample channel, and the entrance end of sample channel corresponds the sample groove setting to receive density gradient centrifugal sample, recovery part sets up at the top of body and sets up to receive the density gradient centrifugal sample through sample channel with the export end of sample channel corresponding, main control unit sets up on the body, controls sample groove to move to separation analysis part to make the density gradient centrifugal sample of different layers in turn enter sample channel and separate, and controls recovery part and receives the density gradient centrifugal sample after separation respectively. The utility model discloses through main control unit control work, makes the density gradient centrifugal sample realize separation after quick through separation analysis part, and simple operation, process time is short, can maximum degree guarantee the accuracy of sample separation, and reduces the pollution of whole separation process, is favorable to the separation and preservation of biological sample. BRIEF DESCRIPTION OF DRAWINGS

[0032] In order to more clearly illustrate the technical scheme in the embodiment of the utility model, the following will be briefly introduced the drawing needed to be used in the embodiment or prior art description, obviously, the drawing in the following description only some embodiments of the utility model, for ordinary skilled person in the art, under the premise of not paying the creative labor, can also obtain other drawings according to these drawings.

[0033] Figure 1 It is the structure explosion schematic view of density gradient centrifugal sample separation and collection device described in the utility model.

[0034] Figure 2 It is the explosion schematic view of density gradient centrifugal sample separation and collection device described in the utility model after separation analysis part is clamped on fixed support.

[0035] Figure 3 It is the side view of density gradient centrifugal sample separation and collection device described in the utility model.

[0036] Figure 4 It is the side view of density gradient centrifugal sample separation and collection device described in the utility model.

[0037] Figure 5 It is the structure explosion schematic view in another embodiment of density gradient centrifugal sample separation and collection device described in the utility model.

[0038] Figure 6 It is the side view in another embodiment of density gradient centrifugal sample separation and collection device described in the utility model.

[0039] Figure 7 A corresponding relationship schematic diagram of the centrifugal tube and the separation analysis part in the density gradient centrifugal sample separation and collection device.

[0040] Figure 8 A spectrum image detected by the separation analysis part in the density gradient centrifugal sample separation and collection device in an embodiment. DETAILED DESCRIPTION

[0041] The density gradient centrifugal sample separation and collection device is provided, so that the purpose, technical scheme and effect of the density gradient centrifugal sample separation and collection device are more clear and definite, and the density gradient centrifugal sample separation and collection device is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the density gradient centrifugal sample separation and collection device, and are not used to limit the density gradient centrifugal sample separation and collection device.

[0042] It should be noted that the terms "center", "upper", "lower", "left", "right", "inner", "outer", "vertical", "horizontal" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only used to facilitate the description of the density gradient centrifugal sample separation and collection device and simplify the description, and do not indicate or imply that the indicated structure must have a particular orientation or must be constructed in a particular orientation, and cannot be understood as a limitation on the density gradient centrifugal sample separation and collection device.

[0043] In addition, unless otherwise specifically limited by the article in the text, "a" and "the" can generally refer to a single or multiple. If the description of "first", "second" and the like is involved in the embodiments of the density gradient centrifugal sample separation and collection device, the description of "first", "second" and the like is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of ordinary skilled in the art, when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, and is not within the protection scope required by the density gradient centrifugal sample separation and collection device.

[0044] Density gradient centrifugation technology is a common method for separating biomolecules, and is widely used in the fields of virus isolation and purification, protein separation, etc. Generally, it includes three steps: first, prepare the density gradient medium in the centrifuge tube, and the sample to be separated is above the medium; use the ultracentrifuge to centrifuge, so that each component in the sample to be separated is layered in the centrifuge tube; finally, collect and detect the sample distribution in each gradient in the medium after centrifugation. After the medium is layered by the density gradient centrifugation method, the components in different layers are generally obtained by cutting method, puncture method and siphon method. The cutting method is to put the centrifuge tube and sample after centrifugation into the freezer for quick freezing, and then cut the frozen centrifuge tube into thin slices with a centrifuge tube cutter, and take out the sample layer by layer, but this method has high cost, long time consumption, and is not suitable for thick-walled tubes or metal tubes; the puncture method uses a needle to puncture the bottom of the centrifuge tube, and the sample is free to drop out by gravity, and then the distribution is collected in turn, but this method also has high cost, and is not suitable for the case where there is a precipitate at the bottom after centrifugation; the siphon method is to insert a small polyethylene tube from the opening of the centrifuge tube until it is inserted into the bottom of the tube, and use the siphon method to separate and absorb the gradient density centrifugation sample, but this method is easy to cause disturbance of the separation zone, affecting the final separation result. Therefore, the existing density gradient centrifugation sample separation method has limitations, and it is difficult to ensure rapid and accurate sample separation results.

[0045] The utility model discloses in order to solve this problem, in order to realize the quick, accurate separation function of density gradient centrifugation sample, provides a density gradient centrifugation sample separation collection device. Figure 1 And Figure 2 As shown in the specific, the density gradient centrifugation sample separation collection device includes: the body 100, separation analysis part 200, recovery part 300 and main control part 400. Wherein, as shown in Figure 1 And Figure 4As shown, the body 100 is internally provided with a sample groove 110 for placing density gradient centrifugation samples; the separation and analysis part 200 is arranged on the top of the body 100, and the inside of the separation and analysis part 200 forms a sample channel 210, the inlet end 211 of the sample channel 210 is arranged corresponding to the sample groove 110 to receive density gradient centrifugation samples; the recovery part 300 is arranged on the top of the body 100 and arranged corresponding to the outlet end 212 of the sample channel 210 to receive density gradient centrifugation samples passing through the sample channel 210; the main control part 400 is arranged on the body 100 to control the sample groove 110 to move towards the separation and analysis part 200 to make different layers of density gradient centrifugation samples enter the sample channel 210 in turn for separation, and to control the recovery part 300 to receive the separated density gradient centrifugation samples respectively. The density gradient centrifugation sample separation device is controlled by the main control part 400 to work, so that the density gradient centrifugation samples are separated after quickly passing through the separation and analysis part 200, the operation is simple, the process time is short, the accuracy of sample separation can be ensured to the greatest extent, the pollution of the whole separation process is reduced, and the separation and preservation of biological samples are facilitated.

[0046] Further, as shown in Figure 1 and Figure 2 , the sample groove 110 includes a placement position 111 and a first motor 112. Wherein, the placement position 111 is arranged at the center position of the sample groove 110, for placing a centrifuge tube containing density gradient centrifugation samples, and the top of the body 100 is provided with an opening 120 corresponding to the placement position 111; the first motor 112 is arranged below the placement position 111, and under the control of the main control part 400, the placement position 111 is pushed to move in the vertical direction to make the centrifuge tube in the placement position 111 pass through the opening 120 to enter the separation and analysis part 200, and different layers of density gradient centrifugation samples in the centrifuge tube are sequentially extruded into the sample channel. The centrifuge tube is pushed by the first motor 112 to move in the vertical direction synchronously with the placement position 111, and the layered samples in the centrifuge tube are sequentially extruded into the separation and analysis part 200 in the order from top to bottom, so as to ensure the order of the samples in each layer entering the separation and analysis part 200, and to ensure that the samples in each layer will not be contaminated, realizing accurate and rapid sample separation.

[0047] In an embodiment, as shown in Figure 5 and Figure 6 , the side of the body 100 is provided with a sliding door 140 corresponding to the position of the sample groove 110, which can be freely opened and closed to facilitate the user to place the centrifuge tube containing density gradient centrifugation samples in the sample groove 110.

[0048] Further, as shown in Figure 4 andFigure 7 As shown, the sample channel 210 in the separation analysis part 200 is in an overall elongated tube structure, and a size gradient changing structure is formed at the inlet end 211 part to match with the centrifuge tube containing the density gradient centrifugation sample at the opening of the inlet end 211, so as to ensure that the density gradient centrifugation sample in the centrifuge tube can enter the sample channel 210 in a layered order. Specifically, the sample channel 210 is in a capillary structure as a whole, and the inlet end 211 is in an inverted bell shape structure towards the sample groove 110, and a gap is formed between the inlet end 211 and the side wall of the separation analysis part 200, wherein the outer diameter of the opening of the inlet end 211 is slightly smaller than the inner diameter of the centrifuge tube, and the inner diameter of the side wall of the separation analysis part 200 is slightly larger than the outer diameter of the centrifuge tube, so that after the centrifuge tube enters the separation analysis part 200, the tube wall of the centrifuge tube is arranged between the inlet end 211 and the side wall corresponding to the gap, while the centrifuge tube is sleeved on the inlet end 211, so as to ensure that when the centrifuge tube continuously moves towards the sample channel 210, the density gradient centrifugation sample in the centrifuge tube enters the sample channel 210 in a layered order from top to bottom, and stable separation between different layer samples is realized.

[0049] Further, as shown in the drawings, Figure 4 The separation analysis part 200 further comprises a sealing ring 220 and an analysis detector 230. The sealing ring 220 is arranged outside the inlet end 211 of the sample channel 210 to be clamped between the inner wall of the centrifuge tube and the outer wall of the inlet end 211 after the centrifuge tube enters the separation analysis part 200, so as to seal the centrifuge tube and the inlet end 211 of the sample channel 210, ensure that the density gradient centrifugation sample does not leak, and ensure that different layer samples can enter the sample channel 210 in sequence, so as to realize stable separation between different layer samples. The analysis detector 230 is arranged on both sides of the sample channel 210 to obtain data of the density gradient centrifugation sample passing through the sample channel 210, so as to determine the time of different layer density gradient centrifugation samples passing through the sample channel 210, and realize separation of the density gradient centrifugation sample. The sealing ring 220 seals the centrifuge tube and the inlet end 211 of the sample channel 210, enhances the sealing between the centrifuge tube and the sample channel 210, improves the accuracy of sample separation, avoids external pollution of the sample, and realizes separation and preservation of the biological sample.

[0050] Further, the analysis detector is a UV detector, which detects the UV spectrum data of the density gradient centrifugation sample passing through the sample channel 210 and records the corresponding passing time, so as to realize the separation of the density gradient centrifugation sample of different components. The components of the sample are identified by forming the UV spectrum, so that even if the impurity layer component or the mutual solubility of the adjacent layer components occurs, the identification and distinction can be quickly performed, and the impurity part and the mutual solubility part are collected as separate components, so as to ensure the purity of the collected layer components and improve the separation accuracy. At the same time, through the rapid UV detection, the sample does not need to stay in the sample channel 210 for a long time, the sample channel 210 can be set as a particularly short flow path, the components of the sample can be accurately determined, so as to reduce the pollution between different layer samples and realize the rapid and accurate sample separation.

[0051] Further, as shown in Figure 1 and Figure 2 , the recovery part 300 includes a test tube rack 310 and a second motor 320. The test tube rack 310 is provided with a plurality of test tube positions 311 for placing a plurality of test tubes; the second motor 320 is arranged below the test tube rack 310 and controls the movement of the test tube rack 310 so that the plurality of test tubes correspond to the outlet end 212 of the sample channel 210 in sequence to receive the separated density gradient centrifugation sample respectively.

[0052] Further, the test tube rack 310 is a disc structure, and the second motor 320 controls the rotation of the test tube rack 310 so that the plurality of test tubes correspond to the outlet end 212 of the sample channel 210 in sequence.

[0053] Further, as shown in Figure 1 and Figure 2 , the main control part 400 includes a main control panel 410 and a main control button 420. The main control panel 410 is arranged on the side of the body 100, receives the data of the density gradient centrifugation sample obtained from the analysis detector 230, and generates a waveform diagram to determine the components of the density gradient centrifugation sample currently flowing through the sample channel 210; the main control button 420 is arranged below the main control panel 410 to control the operation of the density gradient centrifugation sample separation device and set corresponding working parameters. Specifically, the working parameters include the moving speed of the first motor 112 driving the placement position 111, the rotating speed of the second motor 320 driving the test tube rack 310, etc., so as to ensure that the density gradient centrifugation sample can be automatically and accurately separated.

[0054] Further, as shown in Figure 2 and Figure 3As shown in the figure, the density gradient centrifugation sample separation and collection device further comprises a fixing support 500 fixedly arranged on the top of the body 100, used for clamping and fixing the separation and analysis part 200, and fixing the inlet end 211 of the sample channel 210 above the opening 120. The detachable connection of the separation and analysis part 200 and the body 100 is realized through the fixing support 500, so that the height of the separation and analysis part 200 relative to the opening 120 is conveniently adjusted, and the separation and analysis part 200 is conveniently cleaned and replaced after being detached.

[0055] Further, as shown in the figure, Figure 1 and Figure 2 As shown in the figure, the density gradient centrifugation sample separation and collection device further comprises an ultrasonic cleaning part 600 arranged on the top of the body 100, used for cleaning the separation and analysis part 200 after being detached from the fixing support 500. The cleaning of the separation and analysis part 200 is realized through the ultrasonic cleaning part 600, so that the service life of the density gradient centrifugation sample separation and collection device is prolonged. Further, the ultrasonic cleaning part 600 is further provided with a drying device, so as to quickly dry the separation and analysis part 200 after ultrasonic cleaning, and facilitate the next use.

[0056] Further, as shown in the figure, Figure 4 As shown in the figure, the density gradient centrifugation sample separation and collection device further comprises a temperature control part 700 arranged around the body 100, used for controlling the temperature of the density gradient centrifugation sample in the density gradient centrifugation sample separation and collection device. Optionally, the temperature control part 700 is sleeved outside the body 100 through a structure similar to a protective cover, so as to control the temperature of the entire density gradient centrifugation sample separation and collection device, so as to ensure that the temperature of the density gradient centrifugation sample to be separated, the density gradient centrifugation sample in separation and the density gradient centrifugation sample after separation is maintained within a suitable range. The temperature of the density gradient centrifugation sample in the density gradient centrifugation sample separation and collection device is adjusted through the temperature control part 700, so as to ensure that the sample is at the most suitable temperature, thereby avoiding sample inactivation and improving the stability of the sample, and realizing the separation and collection of the biological sample. Optionally, the temperature control part 700 is an air conditioning circulation system, arranged at different positions of the density gradient centrifugation sample separation and collection device, and used for controlling the temperature of the density gradient centrifugation sample in the density gradient centrifugation sample separation and collection device through a compressor.

[0057] The density gradient centrifugation sample separation device is controlled to work through the main control part, so that the density gradient centrifugation sample is quickly separated after passing through the separation and analysis part, the operation is simple, the process time is short, the accuracy of sample separation can be ensured to the greatest extent, the pollution of the entire separation process is reduced, and the separation and preservation of the biological sample are facilitated.

[0058] In one embodiment, as shown in Figure 1 and Figure 4 The density gradient centrifugation sample separation and collection device comprises a body 100, a separation and analysis part 200, a recovery part 300, a main control part 400, a fixed support 500, an ultrasonic cleaning part 600, and a temperature control part 700. In one embodiment, the temperature control part 700 forms a protective cover structure covering the outside of the body 100. The temperature control part 700 covers the body 100 from above and contains the body 100, the separation and analysis part 200, the recovery part 300, the main control part 400, the fixed support 500, and the ultrasonic cleaning part 600, thereby avoiding the influence of the external environment on the components inside the density gradient centrifugation sample separation and collection device and controlling the temperature inside the density gradient centrifugation sample separation and collection device as needed.

[0059] Specifically, as shown in Figure 1 In this embodiment, the body 100 is a hollow box structure. The body top plate 130 of the body 100 can be removed to expose the sample tank 110 arranged inside the body 100. Specifically, the sample tank 110 is a circular tank with a downwardly recessed placement site 111 at the center for placing a centrifuge tube that has been treated by centrifugation and has been stratified by density gradient. The sample tank 110 is contained inside the body 100, thereby avoiding contamination and prolonging the storage time of the density gradient centrifugation components in the sample tank 110. Further, a first motor 112 is arranged below the placement site 111. The first motor 112 can be a stepper motor to control the vertical movement of the placement site 111. An opening 120 is arranged on the body top plate 130 corresponding to the position of the placement site 111, so that when the first motor 112 drives the placement site 111 to move upward, the centrifuge tube placed in the placement site 111 can pass through the opening 120 extending from the body top plate 130.

[0060] Further, in another embodiment, as shown in Figure 5 and Figure 6 A sliding door 140 is arranged on the side of the body 100. When the sliding door 140 is opened, the centrifuge tube containing the density gradient centrifugation sample can be placed in the sample tank 110. When the sliding door 140 is closed, the centrifuge tube placed in the sample tank 110 is sealed in the body 100.

[0061] Further, in another embodiment, the top plate of the body 100 is fixed, a drawer structure is formed on the side of the body 100, and the sample groove 110 is fixed on the drawer structure, so that the centrifugal tube to be processed can be placed on the placement site 111 by pulling out the drawer structure from the body 100.

[0062] Further, as shown in Figure 2 and Figure 3 In the present embodiment, the main control part 400 is arranged on the side of the body 100, and the main control part 400 includes a main control panel 410 and a main control button 420, wherein the main control panel 410 is used to display the data of the density gradient centrifugal sample obtained by the separation and analysis part 200, so that the user can determine the sample component currently flowing out of the separation and analysis part 200, and the main control button 420 is respectively connected with the sample groove 110, the separation and analysis part 200, the recovery part 300, the ultrasonic cleaning part 600 and the temperature control part 700 in communication, so as to control the working parameters of each part of the density gradient centrifugal sample separation and collection device. Optionally, the working parameters include sample injection speed, recovery conditions, temperature conditions and ultrasonic parameters, so as to realize the automatic processing of the density gradient centrifugal sample separation and collection device.

[0063] Further, as shown in Figure 1 In the present embodiment, the temperature control part 700 is arranged around the body 100 by a shield structure. Optionally, the temperature control part 700 is an air conditioner arranged around the body, and the air conditioner is connected with the main control part 400 in communication, so as to control the power of the air conditioner through the main control part 400, thereby controlling the temperature of the density gradient centrifugal sample to be separated, the density gradient centrifugal sample in the separation process and the separated density gradient centrifugal sample in the density gradient centrifugal sample separation and collection device, and ensuring that the corresponding sample is stored at an appropriate temperature.

[0064] Further, as shown in Figure 1 and Figure 2As shown, in this embodiment, a fixing bracket 500 is fixedly provided on the top plate 130 of the main body 100. The fixing bracket 500 is engaged with the separation and analysis section 200, thereby realizing a detachable connection between the separation and analysis section 200 and the main body 100. When the fixing bracket 500 is engaged with the separation and analysis section 200, the separation and analysis section 200 is positioned above the opening 120, ensuring that when the first motor 112 pushes the placement position 111 upward, the centrifuge tube in the placement position 111 can directly extend into the separation and analysis section 200. Optionally, the fixing bracket 500 is a height-adjustable bracket structure to adjust the height of the separation and analysis section 200 relative to the opening 120, thereby accommodating centrifuge tubes of different sizes placed in the placement position 111.

[0065] Furthermore, such as Figure 1 and Figure 2 As shown, in this embodiment, an ultrasonic cleaning section 600 is provided on the top plate 130 of the main body. The ultrasonic cleaning section 600 is an ultrasonic cleaning groove formed on the top surface of the top plate 130 of the main body. After the separation and analysis section 200 is used, the separation and analysis section 200 is removed from the fixed bracket 500 and placed into the ultrasonic cleaning section 600. The main control unit 400 controls the ultrasonic cleaning section 600 to start working, thereby cleaning the separation and analysis section 200 and preventing residual components in the separation and analysis section 200 from affecting the next separation result.

[0066] Furthermore, such as Figure 4As shown, in the present embodiment, a sample channel 210 is formed in the separation analysis part 200 for the density gradient centrifuged sample to pass through, and the density gradient centrifuged sample passing through the sample channel 210 is separated. Specifically, the sample channel 210 is a capillary channel, including an inlet end 211 and an outlet end 212 in the vertical direction, and a connecting pipe 213 connecting the inlet end 211 and the outlet end 212 in the horizontal direction. Among them, the inlet end 211 is arranged above the opening 120 corresponding to the centrifuge tube arranged in the placement site 111, and the inlet end 211 is arranged in an inverted trumpet shape and forms a gap with the side wall of the separation analysis part 200, so that when the first motor 112 drives the placement site 111 to move upward, the tube wall of the centrifuge tube placed in the placement site 111 is clamped in the gap, so that the centrifuge tube is sleeved on the inlet end 211. Further, an elastic sealing ring 220 is arranged outside the inlet end 211, so as to seal the gap between the centrifuge tube and the inlet end 211 after the centrifuge tube enters the separation analysis part 200 and is sleeved on the inlet end 211. In this way, when the first motor 112 drives the placement site 111 to continue to rise, the centrifuge tube is squeezed to completely squeeze the internal density gradient centrifuged components into the inlet end 211 without leaking to the position outside the sample channel. Since the sample channel 210 is a capillary channel, the density gradient centrifuged components enter the sample channel in turn according to the order of stratification from top to bottom and move along the sample channel 210, and finally drip out from the outlet end 212 in order. Further, the sealing ring 220 ensures the sealing of the centrifuge tube and the inlet end 211, avoids air or other impurities from entering the sample channel 210, and thus improves the accuracy of sample separation.

[0067] Further, as Figure 4As shown, in the present embodiment, an analysis detector 230 is arranged at the side of the connecting pipe 213 of the sample channel 210, which is a UV detector, and the composition of the current sample component is determined by detecting the UV spectrum data of the sample component flowing through the specific position of the sample channel 210. Further, the analysis detector 230 sends the acquired UV spectrum data to the main control panel 410, and generates a spectrum diagram on the main control panel 410 to indicate the composition of the current sample component. When the UV spectrum data detected by the analysis detector 230 changes, it indicates that the components of the same layer in the original density gradient centrifugation component have all flowed out of the sample channel 210, and the components of the next layer continue to flow through the sample channel 210. The time when the composition of the components changes is recorded, so as to determine the time when the different components drip out of the outlet end 212, and realize the separation of the components of different layers in the density gradient centrifugation component. Optionally, the density gradient medium in the density gradient centrifugation component is sucrose solution. Further, the composition of the current component is determined by the UV spectrum data. In this case, even if there is mutual solubility between the adjacent layers of the sample to be separated, the different spectrum diagram waveforms on the main control panel 410 can be used to identify and distinguish the mutual solubility part, and the mutual solubility part is collected as a separate component, so as to ensure the purity of the collected components of each layer, avoid the mutual interference between the components of the adjacent layers, and improve the separation accuracy.

[0068] Further, as Figure 1 and Figure 2As shown, in the present embodiment, the recovery part 300 is arranged on the top surface of the body top plate 130 and corresponds to the outlet end 212 of the sample channel 210 in the separation and analysis part 200. Specifically, the recovery part 300 includes a disc-shaped test tube rack 310 and a second motor 320 arranged below the test tube rack 310. The test tube rack 310 is uniformly and circumferentially spaced with a plurality of test tube positions to place a plurality of test tubes to receive different components of the density gradient centrifugation components. Specifically, after the main control part 400 acquires the UV spectrum data collected by the analysis detector 230 in the separation and analysis part 200 and the corresponding time data, the main control part 400 determines the start time and end time of the different components of the density gradient centrifugation components dripping from the sample channel 210, controls the second motor 320 to drive the test tube rack 310 to rotate, so as to ensure that the same test tube corresponds to the outlet end 212 of the sample channel 210 during the dripping time of the same component from the sample channel 210, and receives the corresponding density gradient centrifugation component; and after the same component is completely dripped, the test tube corresponding to the outlet end 212 of the sample channel 210 is switched, so as to ensure that the density gradient centrifugation components of different layers are received by different test tubes, and the separation and collection of the density gradient centrifugation components are completed. In combination with the spectrum generated on the main control panel 410, the components of the density gradient centrifugation components in each test tube can be determined, and the rapid, accurate separation, collection and analysis of the density gradient centrifugation components are realized.

[0069] As Figure 8 As shown, when the corresponding components flow through the sample channel 210 and pass through the analysis detector 230 in sequence during the separation of the yeast ribosome components by the density gradient centrifugation sample separation and collection device, the analysis detector 230 detects and acquires the spectrum data. Specifically, in the present embodiment, the preparation process of the density gradient centrifugation sample to be separated is as follows: first, 10% sucrose solution and 60% sucrose solution are slowly added from the bottom of the SW40 sample tube by using a syringe, and the density gradient centrifugation solution is obtained by standing overnight; then, the yeast cells are centrifuged and quickly ground in liquid nitrogen, and the supernatant containing a large amount of ribosomes is obtained under the condition of 14000 rpm and 4°C for 20 min; then, the supernatant is slowly added to the top of the sucrose density gradient solution, and the ultracentrifuge is used to centrifuge under the condition of 38 krpm and 4°C for 2h40min, and the density gradient centrifugation sample is obtained, and the different components of the ribosomes are distributed in the samples with different densities. The density gradient centrifugation sample in the present embodiment is placed in the sample groove 110 of the density gradient centrifugation sample separation and collection device, the temperature is set to 4°C by the temperature control part 700, the centrifuge tube is fed into the separation and analysis part 200 at a speed of 1 mL / min, and the spectrum as shown in FIG. 9 is obtained. Figure 8The peak graph is shown. According to the wave peak data in the image, the solution containing 40S ribosome small subunit, the solution containing 60S ribosome large subunit, and the solution containing 80S monoribosome are sequentially separated by the separation detector 230 in the separation analysis part 200, and then the solution containing polysome. According to the corresponding order, the host control part 400 controls the second motor 320 to drive the test tube rack 310 to rotate, so that the outlet end 212 of the sample channel 210 is provided with different test tubes in the corresponding time period to collect the corresponding components respectively, so as to complete the rapid and accurate separation, collection and analysis of different yeast ribosome components. The whole process is convenient and fast, and easy to apply.

[0070] In summary, the utility model discloses a density gradient centrifugal sample separation and collection device, include: the body, separation analysis part, recovery part and host control part. In which, the body inside is equipped with sample tank to place density gradient centrifugal sample, separation analysis part sets up at the top of body, the inside of separation analysis part forms sample channel, and the entrance end of sample channel corresponds the sample tank setting to receive density gradient centrifugal sample, recovery part sets up at the top of body and corresponds the outlet end of sample channel setting to receive the density gradient centrifugal sample through sample channel, host control part sets up on the body, control sample tank moves to separation analysis part to make the density gradient centrifugal sample of different layers enter sample channel in proper order and separate, and control recovery part receives the density gradient centrifugal sample after separation respectively. The utility model discloses through host control part control work, makes the density gradient centrifugal sample realize separation after passing through separation analysis part quickly, and simple operation, process time is short, can maximum degree guarantee the accuracy of sample separation, and reduce the pollution of whole separation process, is favorable to the separation and preservation of biological sample.

[0071] The following briefly describes the working process of the density gradient centrifugal sample separation and collection device of the utility model:

[0072] The push-pull door 140 is opened, the centrifugal tube containing the sample to be separated is placed on the placing position 111 of the sample groove 110 in the body 100, the overall environment is ensured to be at a suitable temperature for the components of the sample by the temperature control part 700, the working parameters are set at the main control part 400, and the density gradient centrifugal sample separation and collection device is controlled to work, the first motor 112 drives the placing position 111 to move upward in the vertical direction, the centrifugal tube enters the separation and analysis part 200 through the opening 120 and is sleeved outside the inlet end 211 of the sample channel 210, and the gap between the centrifugal tube and the inlet end 211 is sealed by the sealing ring 220; the first motor 112 continues to drive the placing position 111 to move upward in the vertical direction, and the sample to be separated in the centrifugal tube is squeezed into the sample channel 210; the spectral absorption data of the sample to be separated is collected by the analysis detector 230 in the separation and analysis part 200, a spectral graph is drawn and displayed on the main control panel 410, the components of different layers of the sample to be separated are determined, and the time of each layer of the sample flowing through the sample channel 210 is determined; the second motor 320 in the recovery part 300 drives the test tube rack 310 to rotate, so that different test tubes are used to collect different components when the different components in the sample to be separated drop from the outlet end 212 of the sample channel 210, and the separation, analysis and collection of the components to be separated are completed. After the work is completed, the separation and analysis part 200 is taken off from the fixed support 500, is placed in the ultrasonic cleaning part 600 for ultrasonic cleaning and drying, and is convenient for continuous use next time.

[0073] The centrifugal tube containing the sample to be separated is moved upward, the centrifugal tube is continuously squeezed after being connected with the separation and analysis part, so that the sample to be separated in the centrifugal tube enters the sample channel of the separation and analysis part in the order of stratification from top to bottom. Meanwhile, the spectral data of the sample flowing through the sample channel is detected in real time by the analysis detector, the components of the current sample are determined, the initial time and the end time of the components of different components dropping from the sample channel are determined, different test tubes are used by the recovery part to collect the components of different components, so that the rapid separation, analysis and collection of the sample to be separated are realized. Because the detection speed is fast, the sample channel can be very short, the accuracy of the separated sample can be guaranteed to the maximum extent, and the pollution between samples is reduced. Meanwhile, because the components of the current component are determined by the spectral data, even if there is mutual solubility between adjacent layers of the sample to be separated, the mutual solubility can be quickly identified in the separation and analysis part, and the mutual solubility part is collected as a separate component, so that the purity of each layer of the collected components is ensured, the mutual interference between the components of adjacent layers is avoided, the accuracy of separation is further improved, and the pollution between the finally collected samples is avoided.

[0074] The utility model discloses a density gradient centrifugal sample separation and collection device, including: the body, separation and analysis part, recovery part and main control unit. In which, the body inside is equipped with sample tank to place density gradient centrifugal sample, separation and analysis part sets up the top of body, the inside of separation and analysis part forms sample channel, and the entrance end of sample channel corresponds the sample tank setting to receive density gradient centrifugal sample, recovery part sets up the top of body and corresponds the outlet end of sample channel setting to receive the density gradient centrifugal sample through sample channel, main control unit sets up on the body, controls sample tank moves to separation and analysis part to make the density gradient centrifugal sample of different layers in turn enter sample channel and separate, and controls recovery part and receives the density gradient centrifugal sample after separation respectively. The utility model passes through main control unit control work, makes the density gradient centrifugal sample realize separation after fast through separation and analysis part, and simple operation, process time is short, can maximum degree ensure the accuracy of sample separation, and reduces the pollution of whole separation process, is favorable to the separation and preservation of biological sample.

[0075] The above-described embodiments are only used to illustrate the technical solutions of the utility model, rather than limit them; although the utility model is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that; it still can modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features; and these modifications or replacements are not the essence of the corresponding technical solutions deviate from the spirit and scope of the utility model embodiments technical solutions, and should be included in the protection scope of the utility model.

Claims

1. A density gradient centrifugation sample separation collection device, characterized by, The application relates to a density gradient centrifugation sample separation and analysis device. The device comprises a body, a sample groove arranged in the body for placing a density gradient centrifugation sample, a separation and analysis part arranged at the top of the body, an inlet end of a sample channel formed in the separation and analysis part corresponding to the sample groove for receiving the density gradient centrifugation sample, a recovery part arranged at the top of the body and corresponding to an outlet end of the sample channel for receiving the density gradient centrifugation sample passing through the sample channel, and a main control part arranged on the body for controlling the sample groove to move towards the separation and analysis part so that different layers of the density gradient centrifugation sample enter the sample channel in sequence for separation, and for controlling the recovery part to receive the separated density gradient centrifugation sample. The sample groove comprises a placing position arranged at the center of the sample groove for placing a centrifuge tube containing the density gradient centrifugation sample, and an opening arranged at the top of the body corresponding to the placing position. The first motor is arranged below the placing position and is controlled by the main control part to push the placing position to move in the vertical direction so that the centrifuge tube in the placing position enters the separation and analysis part through the opening, and different layers of the density gradient centrifugation sample in the centrifuge tube are sequentially extruded into the sample channel. In the separation and analysis part, the inlet end of the sample channel is in an inverted trumpet shape, and a gap is formed between the inlet end and the side wall of the separation and analysis part, so that after the centrifuge tube enters the separation and analysis part, the tube wall of the centrifuge tube is located between the inlet end and the side wall and is sleeved on the inlet end, and different layers of the density gradient centrifugation sample in the centrifuge tube are sequentially extruded into the sample channel.

2. The density gradient centrifugation sample separation and collection device of claim 1, wherein, The separation and analysis part further comprises a sealing ring arranged outside the inlet end of the sample channel to seal the centrifuge tube and the inlet end of the sample channel after the centrifuge tube is sleeved on the inlet end, so as to ensure that the density gradient centrifugation sample enters the sample channel in a layered order. The analysis detector is arranged on both sides of the sample channel to acquire data of the density gradient centrifugation sample passing through the sample channel, so as to determine the time of different layers of the density gradient centrifugation sample passing through the sample channel, and realize separation of the density gradient centrifugation sample. The recovery part comprises a test tube rack provided with a plurality of test tube positions for placing a plurality of test tubes, and a second motor arranged below the test tube rack for controlling the test tube rack to move so that the plurality of test tubes correspond to the outlet end of the sample channel in sequence to receive the separated density gradient centrifugation sample.

3. The density gradient centrifugation sample separation and collection device of claim 2, wherein, The test tube rack is in a disc structure, and the second motor controls the test tube rack to rotate so that the plurality of test tubes correspond to the outlet end of the sample channel in sequence.

4. The density gradient centrifugation sample separation and collection device of claim 3, wherein, The main control part comprises a main control panel arranged on the side of the body, which receives the acquired data of the density gradient centrifugation sample from the analysis detector and generates a waveform diagram to determine the composition of the current density gradient centrifugation sample flowing through the sample channel. ​ ​ 5. The density gradient centrifugation sample separation and collection device of claim 4, wherein, ​ ​ ​ 6. The density gradient centrifugation sample separation and collection device of claim 5, wherein, ​ 7. The density gradient centrifugation sample separation and collection device of claim 4, wherein, ​ ​ A master button is arranged below the master panel to control the operation of the density gradient centrifugal sample separation device and set corresponding working parameters.

8. The density gradient centrifugation sample separation and collection device of claim 2, wherein, Further comprising: A fixing support is fixedly arranged on the top of the body to hold the separation and analysis part and fix the inlet end of the sample channel above the opening.

9. The density gradient centrifugation sample separation collection device of claim 8, wherein, Further comprising: An ultrasonic cleaning part is arranged on the top of the body to clean the separation and analysis part after being removed from the fixing support. A temperature control part is arranged around the body to control the temperature of the density gradient centrifugal sample in the density gradient centrifugal sample separation and collection device.

10. The density gradient centrifugation sample separation and collection device of claim 1, wherein, The side of the body is provided with a sliding door to place the centrifuge tube containing the density gradient centrifugal sample into the sample tank.

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