Extraction device for cast-off cells

Abstract

The utility model discloses an exfoliated cell extraction device. The exfoliated cell extraction device comprises an extraction tube capable of extracting exfoliated cells; the inner sleeve can be used for placing a textile containing cast-off cells, and the inner sleeve can be arranged in the extraction tube. The inner sleeve disclosed by the utility model can be used for accommodating more textile fabrics, so that enough cast-off cells can be extracted, and enough DNA (Deoxyribose Nucleic Acid) templates can be obtained.

Description

Technical Field

[0001] This utility model belongs to the field of investigation technology, specifically relating to a device for extracting exfoliated cells. Background Technology

[0002] DNA extraction is typically required during criminal investigation and evidence collection. Various methods are generally used to extract DNA from textiles, including cutting, adhesion, and negative pressure aspiration enrichment. When the textile is dry and free of oil, any of these methods can be freely chosen. However, for damp or oily textiles, the characteristics of the sample itself limit the effectiveness of adhesion and negative pressure aspiration enrichment methods, leaving only cutting. However, the cutting method is limited by the 1.5mL centrifuge tube capacity, resulting in insufficient textile material to collect enough exfoliated cells and thus insufficient DNA template, leading to PCR amplification failure. Utility Model Content

[0003] This invention addresses the technical problem that existing exfoliated cell extraction devices cannot cut enough textiles, resulting in too few exfoliated cells collected and insufficient DNA template, thus leading to PCR amplification failure. The purpose is to provide an exfoliated cell extraction device.

[0004] The exfoliated cell extraction device of this utility model includes:

[0005] Extraction tubes that can extract exfoliated cells;

[0006] An inner sleeve containing textiles with shed cells can be placed inside the extraction tube.

[0007] Preferably,

[0008] The exfoliated cell extraction device also includes:

[0009] A cap may be placed over the opening of the extraction tube.

[0010] Preferably,

[0011] The bottom of the extraction tube that can extract exfoliated cells is a cavity for collecting exfoliated cells, and the upper surface of the cavity is a support surface that suspends and supports the inner tube.

[0012] Preferably,

[0013] The inner sleeve has:

[0014] A tube body for holding textiles containing shed cells, the bottom of the tube body having a perforation.

[0015] Preferably,

[0016] The inner sleeve also has:

[0017] A lifting ring has one end connected to the opening of the tube body, and the other end can be close to the opening of the extraction tube; preferably, the other end of the lifting ring has a pull ring for lifting. More preferably,

[0018] The extraction tube is a centrifuge tube, and the conical bottom cavity of the centrifuge tube is a bottom cavity for collecting exfoliated cells. The upper surface of the conical bottom cavity is a support surface for suspending and supporting the inner sleeve.

[0019] Alternatively, the extraction tube may be a test tube, with a hemispherical bottom cavity that can collect exfoliated cells, and the upper surface of the hemispherical bottom cavity serving as a support surface that suspends and supports the inner tube.

[0020] Preferably,

[0021] The drain hole at the bottom of the tube body is a grid of small holes.

[0022] Preferably,

[0023] The tube cap has a threaded opening.

[0024] Preferably,

[0025] The height of the extraction tube is 3 to 5 times the height of the inner sleeve;

[0026] The pull ring of the lifting ring is close to the opening of the extraction tube.

[0027] Preferably,

[0028] The exfoliated cell extraction device further includes an enrichment tube for transferring the exfoliated cells extracted from the extraction tube. Preferably, the extraction tube is a centrifuge tube with a large inner diameter, and the enrichment tube is a centrifuge tube with a small inner diameter.

[0029] The positive and progressive effects of this utility model are as follows:

[0030] 1) This utility model uses an inner sleeve that can hold textiles containing exfoliated cells, which can accommodate a large amount of textiles so as to extract enough exfoliated cells and thus obtain a sufficient amount of DNA template. Attached Figure Description

[0031] Figure 1 This is a perspective view of the exfoliated cell extraction device of this utility model. Detailed Implementation

[0032] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model.

[0033] like Figure 1 As shown, the exfoliated cell extraction device of this utility model includes an extraction tube 10, an inner sleeve 20 that can be placed inside the extraction tube 10, and a tube cap 30 that can be placed on the opening of the extraction tube 10.

[0034] In this example, the extraction tube 10 can be a centrifuge tube or a test tube, such as a ready-made 15 ml centrifuge tube or test tube with a cap. The conical bottom of the centrifuge tube is a cavity for collecting exfoliated cells, and the upper surface of the conical bottom cavity is a support surface for suspending and supporting the inner tube. The hemispherical bottom of the test tube is a cavity for collecting exfoliated cells, and the upper surface of the hemispherical bottom cavity is a support surface for suspending and supporting the inner tube. In this way, the inner tube 20 can always be kept suspended. The extraction tube 10 can be made of plastic or glass. The inner tube 20 has a tube body 21 for placing a textile containing exfoliated cells. The bottom of the tube body 21 has a perforation that allows exfoliated cells to enter the extraction tube 10. The perforation is, for example, a mesh-like pore. The inner sleeve 20 also has a lifting ring 22, one end of which has a pull ring 221 for lifting, near the opening of the extraction tube 10, and the other end of which is connected to the opening of the tube body 21. The tube cap 30 has a threaded opening 31. In this example, the height of the extraction tube can be 3 to 5 times the height of the inner sleeve. This device utilizes a mixer and centrifuge to achieve the extraction and enrichment of exfoliated cells, and is also suitable for the extraction and enrichment of exfoliated cells from dried textiles.

[0035] Example 1

[0036] In use, place the inner tube 20 into the extraction tube 10, place the cut large piece of textile into the inner tube body 21, add sufficient EDTA (8.0) until the sample is submerged, cover with the tube cap 30, place on a constant temperature mixer, and mix at room temperature for 1 hour. Thorough mixing can separate the exfoliated cells from the textile. Then, centrifuge for 5 minutes to centrifuge the exfoliated cells to the bottom of the extraction tube 10. Then, open the tube cap 30, pull the lifting arm ring 22, and pull out the inner tube body 21 along with the textile. Next, use a pipette to aspirate the supernatant in the extraction tube 10, leaving only the bottom sediment. Then, transfer the sediment to a 1.5 ml centrifuge tube, add lysis buffer, and perform DNA extraction and purification experiments.

[0037] Example 2

[0038] When using this method, for textiles with a large amount of oil or moisture, add EDTA (8.0) until the textile is submerged, then cover the tube with cap 30 and heat it at 56 degrees Celsius for 2 hours in a constant temperature mixer to fully separate the oil, textile, and exfoliated cells. Then centrifuge for 5 minutes to centrifuge the exfoliated cells to the bottom of the extraction tube 10. Then open the tube cap 30, pull the lifting arm ring 22, and pull out the inner tube body 21 along with the textile. Next, use a pipette to aspirate the supernatant in the extraction tube, leaving only the bottom sediment. Then transfer the sediment to a 1.5 ml centrifuge tube, add lysis buffer, and perform DNA extraction and purification experiments.

[0039] The present invention has been described in detail above with reference to the accompanying drawings and embodiments. Those skilled in the art can make various modifications to the present invention based on the above description. Therefore, certain details in the embodiments should not be construed as limiting the present invention, and the scope of protection of the present invention shall be defined by the appended claims.

Claims

1. A device for extracting exfoliated cells, characterized in that... The exfoliated cell extraction device includes: Extraction tubes that can extract exfoliated cells; An inner sleeve can be placed inside the extraction tube to hold textiles containing exfoliated cells. The bottom of the extraction tube that can extract exfoliated cells is a cavity for collecting exfoliated cells, and the upper surface of the cavity is a support surface that suspends and supports the inner tube.

2. The exfoliated cell extraction device as described in claim 1, characterized in that... The exfoliated cell extraction device also includes: A cap may be placed over the opening of the extraction tube.

3. The exfoliated cell extraction device as described in claim 1, characterized in that... The inner sleeve has: A tube body for holding textiles containing shed cells, the bottom of the tube body having a perforation.

4. The exfoliated cell extraction device as described in claim 3, characterized in that... The inner sleeve also has: The lifting ring has one end connected to the opening of the tube body and the other end close to the opening of the extraction tube.

5. The exfoliated cell extraction device as described in claim 4, characterized in that... The other end of the lifting ring has a pull ring for lifting.

6. The exfoliated cell extraction device as described in claim 1, characterized in that... The extraction tube is a centrifuge tube, and the conical bottom cavity of the centrifuge tube is a bottom cavity for collecting exfoliated cells. The upper surface of the conical bottom cavity is a support surface for suspending and supporting the inner sleeve. Alternatively, the extraction tube may be a test tube, with a hemispherical bottom cavity that can collect exfoliated cells, and the upper surface of the hemispherical bottom cavity serving as a support surface that suspends and supports the inner tube.

7. The exfoliated cell extraction device as described in claim 3, characterized in that... The drain hole at the bottom of the tube body is a grid of small holes.

8. The exfoliated cell extraction device as described in claim 2, characterized in that... The tube cap has a threaded opening.

9. The exfoliated cell extraction device as described in claim 4, characterized in that... The height of the extraction tube is 3 to 5 times the height of the inner sleeve; The pull ring of the lifting ring is close to the opening of the extraction tube.

10. The exfoliated cell extraction device as described in claim 1, characterized in that... The exfoliated cell extraction device also includes an enrichment tube for transferring exfoliated cells extracted from the extraction tube.

11. The exfoliated cell extraction device as described in claim 10, characterized in that... The extraction tube is a centrifuge tube with a large inner diameter, and the enrichment tube is a centrifuge tube with a small inner diameter.

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