Slide for manufacturing malaria blood slide

Abstract

The utility model discloses a slide for manufacturing malaria blood slides, which relates to the technical field of medical equipment and comprises a glass slide, a slide pushing piece and a slide pushing piece mounting device. Marking grid lines are arranged at the positions, corresponding to the thick blood film circular groove and the thin blood film preparation area, of the back face of the glass slide respectively, the slide glass installation device comprises an installation frame, angle adjusters, a sliding block and a slide glass clamping device, the two ends of the installation frame are each connected with one angle adjuster, and the angle adjusters are connected with the sliding block. Each angle adjuster is connected with one sliding block, the two sliding blocks are arranged at the two ends of the glass slide in a sliding mode respectively, and the glass pushing slide clamping device is arranged on the mounting frame and used for clamping and fixing the glass pushing slide. The device is suitable for manufacturing malaria blood smears, positioning and marking are convenient, and the manufacturing quality of the blood smears is improved.

Description

Technical Field

[0001] This utility model relates to the field of medical equipment technology, and in particular to a glass slide for making malaria blood smears. Background Technology

[0002] Blood smear microscopy is the gold standard for malaria diagnosis recognized by the World Health Organization. This method is simple to perform, low in cost, and can quickly identify the species and stage of the parasites, as well as calculate the density of protozoan infection. It is suitable for use in laboratories at all levels. The quality of malaria blood smears is crucial in malaria diagnosis, and smear preparation is a key step in determining the quality of malaria blood smears.

[0003] Currently, malaria blood smear preparation mainly relies on manual methods. The slide preparation machines widely used in hospitals can only produce thin blood films, not thick ones, which does not meet industry standards for malaria blood smear preparation. Manual blood smear preparation often requires two slides: one as the base slide and the other as the pusher slide. If the practitioner lacks sufficient skill during the preparation process, it is often impossible to produce a uniformly thick, circular blood film. Furthermore, the pusher slide is prone to misalignment with the base slide during preparation, and the angle and force of the pusher are difficult to control, making it challenging to produce a tongue-shaped thin blood film of suitable size and uniform thickness.

[0004] Existing technologies also include glass slides for preparing blood smears, such as the utility model patent entitled "A Novel Glass Slide for Preparing Blood Film Smears" with application number "202320565265.0". This slide has a drip hole and a filling pool. Blood is added to the drip hole and flows through the blood groove to the filling pool, automatically diffusing to form a tongue-shaped blood film. This method eliminates the need for manual slide pushing and is convenient to operate. However, it can only produce a thin, tongue-shaped blood film, not a thick one, which does not meet the industry standards and work specifications for malaria blood smear preparation. Furthermore, this slide requires a relatively large amount of blood (30 microliters) for it to diffuse and form a film on its own, while the preparation of malaria blood smears requires only 1-1.5 microliters of blood for a thin blood film, which is insufficient to produce a thin blood film using this slide. The utility model patent with application number "202220870194.0" provides an adjustable-angle manual slide. By adjusting the angle between the slide and the slide in a timely manner, a blood smear with consistent thickness and good uniformity can be obtained. However, this slide can only produce thin blood films and cannot achieve the function of marking and locating the target body during microscopic examination.

[0005] Given the current challenges in the preparation and microscopic examination of malaria blood smears, there is an urgent need in this field for a glass slide suitable for preparing malaria blood smears, which would facilitate positioning and marking, and improve the quality of blood smear preparation. Utility Model Content

[0006] The purpose of this invention is to provide a glass slide for preparing malaria blood smears, thereby solving the problems existing in the prior art. It is suitable for preparing malaria blood smears, facilitates positioning and marking, and improves the quality of blood smear preparation.

[0007] To achieve the above objectives, this utility model provides the following solution:

[0008] This utility model provides a glass slide for preparing malaria blood smears, including a glass slide, a slide pusher, and a slide pusher mounting device. The glass slide has a thick blood film circular groove and a thin blood film preparation area. The back of the glass slide has marking grid lines corresponding to the thick blood film circular groove and the thin blood film preparation area. The slide pusher mounting device includes a mounting frame, an angle adjuster, a slider, and a slide pusher holder. An angle adjuster is connected to each end of the mounting frame, and a slider is connected to each angle adjuster. The two sliders are slidably disposed at both ends of the glass slide. The slide pusher holder is disposed on the mounting frame and is used to clamp and fix the slide pusher.

[0009] Preferably, the slide holder includes a spring and two opposing clamping plate assemblies. The mounting frame has two slide rails at both ends, each corresponding to one of the two clamping plate assemblies. Each clamping plate assembly includes a clamping plate, a slider, and a limiting block. The clamping plate and the limiting block are respectively disposed on both sides of the mounting frame. The slider passes through the slide rail, and both ends of the slider are fixedly connected to the clamping plate and the limiting block, respectively. The two limiting blocks are connected by the spring.

[0010] Preferably, the glass slide has a label marking area.

[0011] Preferably, the slide has a buffer blocking region that separates the thick blood film circular groove from the thin blood film preparation area.

[0012] Preferably, the buffer blocking area is configured as an elongated groove.

[0013] Preferably, the other two ends of the glass slide are provided with anti-slip areas.

[0014] Preferably, each small square in the marked grid line is composed of different line segments.

[0015] Preferably, the angle adjuster includes a fixed shaft, a rotating sleeve, and a clamping knob. One end of the fixed shaft is fixedly connected to the slider, and the other end is threaded to the clamping knob. The rotating sleeve is disposed on the fixed shaft and located between the slider and the clamping knob. Tightening the clamping knob can press the rotating sleeve onto the slider. Both ends of the mounting bracket are fixedly connected to the rotating sleeves of the two angle adjusters, respectively.

[0016] The present invention achieves the following technical advantages over the prior art:

[0017] This invention provides a glass slide for preparing malaria blood smears. The slide has a circular groove for creating a thick blood film, facilitating the production of a uniform diameter and thickness of the circular thick blood film. A slide-mounting device is used to install a slide, allowing for easy adjustment and manipulation. This produces a uniform, tongue-shaped thin blood film in the thin blood film preparation area. The process is convenient and easy, overcoming the problem of slide failure due to insufficient operator skill or technique, and greatly improving the quality of malaria blood smear preparation. Marking grid lines are provided on the back of the slide at corresponding positions to the circular groove for the thick blood film and the thin blood film preparation area. These lines are used for marking and locating the specimen on the slide, facilitating convenient positioning and rapid tracking. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of the glass slide used to prepare malaria blood smears in an embodiment of this utility model;

[0020] Figure 2 This is a schematic diagram of the structural connection between the glass slide and the glass slide mounting device in an embodiment of this utility model;

[0021] Figure 3 This is a schematic diagram of the structure of the slide holder in an embodiment of the present invention;

[0022] Figure 4 This is a schematic diagram of the connection structure of the mounting bracket, angle adjuster, and slider in an embodiment of this utility model;

[0023] Figure 5 This is a schematic diagram of the angle adjuster in an embodiment of the present invention;

[0024] Figure 6 This is a schematic diagram of the front structure of the glass slide in an embodiment of this utility model;

[0025] Figure 7 This is a schematic diagram of the grid lines marked on the back of the glass slide in an embodiment of this utility model.

[0026] In the diagram: 1-Slide, 2-Slide pusher, 3-Slide pusher mounting device, 31-Mounting bracket, 311-Slide rail, 32-Angle adjuster, 321-Fixed shaft, 322-Rotating sleeve, 323-Pressure knob, 33-Slider, 34-Slide pusher holder, 341-Clamping plate assembly, 3411-Clamping plate, 3412-Slide rod, 3413-Limiting block, 342-Spring, 4-Thick blood film circular groove, 5-Thin blood film preparation area, 6-Marking grid line, 7-Label marking area, 8-Buffer blocking area, 9-Anti-slip area, 10-Small square. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] The purpose of this invention is to provide a glass slide for preparing malaria blood smears, thereby solving the problems existing in the prior art. It is suitable for preparing malaria blood smears, facilitates positioning and marking, and improves the quality of blood smear preparation.

[0029] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] like Figures 1-7 As shown, this embodiment provides a slide for preparing malaria blood smears, including a slide 1, a pusher slide 2, and a pusher slide mounting device 3. The slide 1 has a thick blood film circular groove 4 and a thin blood film preparation area 5. The back of the slide 1 is provided with marking grid lines 6 at positions corresponding to the thick blood film circular groove 4 and the thin blood film preparation area 5. The pusher slide mounting device 3 includes a mounting frame 31, an angle adjuster 32, a slider 33, and a pusher slide holder 34. An angle adjuster 32 is connected to each end of the mounting frame 31, and a slider 33 is connected to each angle adjuster 32. The two sliders 33 are slidably disposed at both ends of the slide 1. The pusher slide holder 34 is disposed on the mounting frame 31 and is used to clamp and fix the pusher slide 2.

[0031] The glass slide 1 is made of glass with a persistent positive charge and adhesion, facilitating the even spreading of the staining solution and improving slide quality. Slide 1 is thin, light-transmitting, and provides high-definition imaging. Slide 1 measures 75mm in length, 25mm in width, and 1.0mm in thickness. A circular groove 4 for thick blood film is provided on slide 1, with a diameter of 10mm and a depth of 0.06–0.07mm, facilitating the production of uniformly thick, 10mm diameter circular blood films. A slide pusher 2 is mounted on slide 1 via a slide pusher mounting device 3, allowing for easy operation and adjustment. This facilitates the production of uniformly thick, appropriately sized, tongue-shaped thin blood films in the thin blood film preparation area 5, simplifying the process and overcoming the problem of slide failure due to insufficient operator skill, thus greatly improving the quality of malaria blood film preparation.

[0032] In this embodiment, the slide holder 34 includes a spring 342 and two opposing clamping plate assemblies 341. The mounting frame 31 has two slide rails 311 at both ends, each corresponding to one of the clamping plate assemblies 341. Each clamping plate assembly 341 includes a clamping plate 3411, a sliding rod 3412, and a limiting block 3413. The clamping plate 3411 and the limiting block 3413 are respectively located on both sides of the mounting frame 31. The sliding rod 3412 passes through the slide rail 311, and its two ends are fixedly connected to the clamping plate 3411 and the limiting block 3413, respectively. The two limiting blocks 3413 are connected by the spring 342. The two clamping plate assemblies 341 slide closer or further apart in the slide rail 311 via the sliding rod 3412, thereby clamping or releasing the slide 2. The spring 342 provides the elastic force for clamping the slide 2. The clamping plate 3411 and the limiting block 3413 are respectively disposed on both sides of the mounting frame 31 and connected to each other by the slide rod 3412, which ensures the installation stability of the clamping plate assembly 341 and the mounting frame 31, and makes the clamping plate assembly 341 move only in a straight line along the slide rail 311.

[0033] In this embodiment, the slide 1 has a label marking area 7 for writing the subject's number and date, etc. The label marking area 7 is a rectangular area with a size of 1cm × 2.5cm.

[0034] In this embodiment, a buffer blocking region 8 is provided on the slide 1, which separates the circular groove 4 of the thick blood film and the thin blood film preparation area 5. In this embodiment, the buffer blocking region 8 is set as an elongated groove. The elongated groove is a shallow rectangular groove with dimensions of length × width × depth = 2.5cm × 0.3cm × 0.05cm. The elongated groove can prevent other liquids from diffusing into the thin blood film area when the blood in the thick blood film (or the thin blood film fixed with methanol and the thick blood film dissolved with distilled water during staining) is present. Especially when the slide 1 is tilted, it can effectively prevent the flow and diffusion of liquid between the thick and thin blood films, ensuring the relative independence of the thick and thin blood films during the preparation and staining process, and preventing them from affecting each other.

[0035] In this embodiment, anti-slip areas 9 are provided at both ends of the slide 1. The anti-slip areas 9 have an uneven texture to increase friction and achieve the purpose of anti-slip. This facilitates manual operation and prevents the slide 1 from slipping or breaking. The anti-slip areas 9 are rectangular areas, with the left anti-slip area 9 measuring 0.5cm × 2.5cm and the right anti-slip area 9 measuring 1cm × 2.5cm.

[0036] In this embodiment, each small square 10 in the marked grid line 6 is composed of different line segments. Several small squares 10 of equal size are set on the back of the slide 1 at the corresponding positions of thick and thin blood films. Each small square 10 is composed of different line segments, allowing for quick location of the small square 10 area during microscopic examination by analyzing the line segments in the field of view. The small square 10 is 0.1cm × 0.1cm in size; that is, there are 100 small squares 10 of 0.1cm × 0.1cm in the area corresponding to the thick blood film, and 500 small squares 10 of 0.1cm × 0.1cm in the area corresponding to the thin blood film. The small squares 10 are composed of different line segments, which can be adjusted according to actual needs, such as different thicknesses, colors, or patterns. The number of small squares 10 corresponding to the thick and thin blood films can be set according to specific work requirements, ensuring that the area of ​​the small square 10 can be determined through the combination of line segments, achieving the purpose of rapid target marking and positioning, without affecting the microscopic examination efficiency due to a large number of small squares 10 causing blurred vision.

[0037] In this embodiment, the angle adjuster 32 includes a fixed shaft 321, a rotating sleeve 322, and a clamping knob 323. One end of the fixed shaft 321 is fixedly connected to the slider 33, and the other end is threadedly connected to the clamping knob 323. The rotating sleeve 322 is sleeved on the fixed shaft 321 and located between the slider 33 and the clamping knob 323. Tightening the clamping knob 323 can press the rotating sleeve 322 onto the slider 33. Both ends of the mounting bracket 31 are fixedly connected to the rotating sleeves 322 in the two angle adjusters 32. By rotating the mounting bracket 31, the rotating sleeve 322 rotates on the fixed shaft 321 to adjust the push glass slide 2 to a suitable angle. After adjustment, tightening the clamping knob 323 presses the rotating sleeve 322 onto the slider 33, thus locking the rotating sleeve 322.

[0038] The specific steps are as follows:

[0039] Labeling: Remove slide 1 and write the subject number and date in labeling area 7.

[0040] To prepare a thick blood film: Take 5 μL of anticoagulated blood with a dropper and drop it into the circular groove 4 of the thick blood film. Use the apex of one corner of the slide 2 to rotate 2-4 times within the circular groove 4 to spread the thick blood film.

[0041] To prepare a thin blood film: Take 1.5 μL of anticoagulated blood with a dropper and add it to the thin blood film preparation area 5 of the slide 1. Install the slide mounting device 3 onto the slide 1. Place the slide 2 on the slide holder 34 and fix it to prevent the slide 2 from deflecting to the left or right. Adjust the angle adjuster 32 according to the blood viscosity to adjust the slide 2 to a suitable angle. Push the slider 33 at a uniform speed to complete the preparation of the thin blood film.

[0042] Staining and Microscopic Examination: After slide preparation, remove the slide mounting device 3 and proceed with the blood smear staining step. After staining and drying, the smear can be directly examined under a microscope. If protozoa are found during microscopic examination, the position of the small square 10 can be determined based on the different line segments, thus achieving marking and localization. If a re-examination is performed, the corresponding protozoan target can be found directly based on the position of the marked small square 10.

[0043] The glass slide for preparing malaria blood smears provided by this utility model has the following advantages:

[0044] 1. It mainly consists of a glass slide, a slide pusher, and a slide pusher mounting device. It does not require expensive and sophisticated instruments, which can save a lot of economic costs. It is easy to operate and has a low cost, making it very suitable for use in hospitals, disease control centers, universities and other research institutions at all levels, especially primary healthcare institutions.

[0045] 2. A circular groove for creating a thick blood film is provided on the slide. The groove has a fixed size and diameter. Creating a thick blood film in this area directly yields a circular blood film with a uniform diameter and thickness. The slide mounting device can be separated from or combined with the slide. To improve the quality of the thin blood film, the slide mounting device can be installed on the slide. By limiting the left and right offset of the slide and controlling the angle between the slide and the slide, the difficulty of manual slide preparation is reduced, thus improving the quality of the blood film.

[0046] 3. The slide has small squares formed by different combinations of line segments on the back of the corresponding areas of thick and thin blood films. During microscopic examination, the position of the small squares can be determined through the field of view under the microscope, enabling rapid tracking and positioning of the target. This is very beneficial for repeated verification tests or training in the workplace, especially when the density of parasites is low or the morphology is atypical. This function can improve the detection rate and save a lot of time and labor costs.

[0047] 4. Anti-slip areas are provided on both sides of the slide to facilitate manual operation and prevent the slide from slipping or breaking.

[0048] 5. The distribution of thick and thin blood films on the slide is clear, reducing the risk of unqualified blood smears due to incorrect distribution of thick and thin blood films.

[0049] 6. The slide has a buffer zone for thick and thin blood films, which can effectively block the diffusion and flow of blood between the thick and thin blood films, or during the staining process, when methanol fixes the thin blood film and distilled water dissolves the thick blood film, ensuring the relative independence of the two areas during slide preparation and staining, and obtaining high-quality thick and thin blood films.

[0050] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A slide for making a malaria blood film, characterized by: The application relates to a slide, a push slide and a push slide mounting device, wherein the slide is provided with a thick blood film circular groove and a thin blood film preparation area, the back surface of the slide is provided with mark grid lines at positions corresponding to the thick blood film circular groove and the thin blood film preparation area, the push slide mounting device comprises a mounting frame, an angle adjuster, a sliding block and a push slide holder, the mounting frame is provided with the angle adjuster at two ends, the angle adjuster is provided with the sliding block at one end, the sliding block is slidably arranged at the two ends of the slide, and the push slide holder is arranged on the mounting frame and used for clamping and fixing the push slide.

2. The slide for making a malaria blood film according to claim 1, wherein: The push slide holder comprises a spring and two oppositely arranged clamping plate assemblies, the mounting frame is provided with two sliding channels corresponding to the two clamping plate assemblies at two ends, the clamping plate assembly comprises a clamping plate, a sliding rod and a limiting block, the clamping plate and the limiting block are arranged at two sides of the mounting frame, the sliding rod penetrates through the sliding channel, the sliding rod is fixedly connected with the clamping plate and the limiting block at two ends, and the two limiting blocks are connected through the spring.

3. The slide for making a malaria blood film according to claim 1, wherein: The slide is provided with a label mark area.

4. The slide for making a malaria blood film according to claim 1, wherein: The slide is provided with a buffer blocking area which separates the thick blood film circular groove and the thin blood film preparation area.

5. The slide for making a malaria blood film according to claim 4, wherein: The buffer blocking area is arranged as a long strip-shaped groove.

6. The slide for making a malaria blood film according to claim 1, wherein: The other two end edges of the slide are provided with anti-skid areas.

7. The slide for making a malaria blood film according to claim 1, wherein: Each small square of the mark grid lines is composed of different line segments.

8. The slide for preparing a malaria blood film according to claim 1, wherein: The angle adjuster comprises a fixed shaft, a rotating sleeve and a compression knob, one end of the fixed shaft is fixedly connected with the sliding block, the other end is threadedly connected with the compression knob, the rotating sleeve is arranged on the fixed shaft and located between the sliding block and the compression knob, the rotating sleeve can be compressed on the sliding block by tightening the compression knob, and the two ends of the mounting frame are fixedly connected with the rotating sleeves of the two angle adjusters.

Images