Placenta tissue stem cell filtering device

Abstract

The utility model discloses a placenta tissue stem cell filtering device and relates to the technical field of filtering devices. The placenta tissue stem cell filtering device comprises a filtering box, a fixing frame and a receiving box which are fixedly arranged in the filtering box, a plurality of groups of filtering nets arranged in the fixing frame and a feeding pipe connected with the filtering box, and a frame used for supporting the filtering nets is fixedly arranged on the inner wall of the fixing frame. According to the device, the material receiving box is arranged at the outlet end of the feeding pipe, and the first flow guide plate is arranged between the material receiving box and the filter screen, so that the feeding path of the cell suspension can be limited, the feeding speed of the cell suspension is slowed down, and the filtering time between the cell suspension and the filter screen is prolonged; the surface of the filter screen can be subjected to unblocking operation in the reciprocating movement process of the material receiving box, so that the overall filtering rate is effectively improved.

Description

Technical Field

[0001] This utility model relates to the field of filtration device technology, specifically a placental tissue stem cell filtration device. Background Technology

[0002] In the medical field, placental tissue stem cells can be used to construct models of various tissues and organs in the laboratory, providing an important tool for studying the development, physiological functions, and disease mechanisms of tissues and organs. For example, constructing a liver model can be used to study the pathophysiological processes and drug metabolism mechanisms of liver diseases, providing an experimental basis for developing new treatment methods and drug screening.

[0003] Currently, filtration methods are used to remove excess tissue from placental stem cells to obtain pure stem cells. After pretreatment, the placental tissue is made into a cell suspension, which enters the filtration device through a feeding pipe. However, the outlet diameter of the feeding pipe is fixed. When the outlet diameter is smaller than the size of the filter screen in the filtration device, the cell suspension fed onto the filter screen will accumulate in one place on the filter screen, and a lot of excess tissue will accumulate on the filter screen. Over time, the filter screen pores become clogged, which affects the overall filtration efficiency. To address the shortcomings of existing technologies, we propose a placental stem cell filtration device to solve the above problems. Utility Model Content

[0004] To address the shortcomings of existing technologies, this utility model provides a placental tissue stem cell filtration device, which solves the problem that when the diameter of the feed pipe outlet port is smaller than the size of the filter screen in the filtration device, the cell suspension fed to the filter screen will accumulate in one place on the filter screen, and a lot of excess filtered tissue will accumulate on the filter screen. Over time, the filter screen pores will become clogged, which will affect the overall filtration efficiency.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a placental tissue stem cell filtration device, comprising a filter box, a fixed frame and a receiving box fixedly disposed inside the filter box, filter screens disposed inside the fixed frame, and a feeding pipe connected to the filter box; an auxiliary mechanism is provided above the multiple sets of filter screens, the auxiliary mechanism comprising:

[0006] The receiving box has a guide box for positioning and moving the receiving box fixedly installed on the top surface of the fixed frame, and the receiving box is fixedly connected to one end of the feeding pipe.

[0007] The first guide plate and the first scraper are both fixedly installed on the bottom surface of the receiving box;

[0008] The second guide plate and the second scraper are slidably disposed on the top surface of a set of filter screens;

[0009] The first screw is rotatably mounted inside the guide box, and a forward and reverse motor for bidirectional rotation of the first screw is fixedly mounted on the side wall of the filter box;

[0010] The second screw, which is rotatably disposed inside the fixed frame, drives the second guide plate and the second scraper to reciprocate along the top surface of a set of filter screens.

[0011] Preferably, the inner wall of the fixed frame is fixedly provided with a frame for supporting the filter screen, and the lengths of the first guide plate, the first scraper, the second guide plate, and the second scraper are equal to the inner diameter of the frame.

[0012] Preferably, the bottom ends of the first guide plate and the second guide plate do not contact the surface of the filter screen, while the bottom ends of the first scraper and the second scraper are in contact with the surface of the filter screen.

[0013] Preferably, a connecting assembly is provided at one end of the first screw and the second screw extending outside the filter box, the connecting assembly being used for linkage between the first screw and the second screw.

[0014] Preferably, the connecting assembly includes pulleys fixedly disposed at one end of the first screw and the second screw, and a connecting belt sleeved between two adjacent sets of pulleys.

[0015] Preferably, a cover for connecting belt shielding is fixedly provided on the side of the filter box.

[0016] Preferably, guide rods are fixedly connected inside both the guide box and the fixing frame.

[0017] This utility model discloses a placental tissue stem cell filtration device, which has the following beneficial effects:

[0018] 1. The placental tissue stem cell filtration device, by setting a receiving box at the outlet end of the feeding pipe and setting a first guide plate between the receiving box and the filter screen, can limit the feeding path of the cell suspension, slow down the feeding speed of the cell suspension, increase the filtration time between the cell suspension and the filter screen, and in combination with the first scraper, can perform a cleaning operation on the surface of the filter screen during the reciprocating movement of the receiving box, effectively improving the overall filtration rate.

[0019] 2. The placental tissue stem cell filtration device uses a connecting belt to link the first guide plate, the first scraper, the second guide plate, and the second scraper, enabling uniform filtration of cell suspension on the surface of each set of filter screens inside the filtration box, and simultaneously clearing blockages on the surface of each set of filter screens, thereby improving the filtration quality of placental tissue stem cells. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 This is a schematic diagram of the filter box and the internal structure of the cover of this utility model;

[0023] Figure 3 This is a schematic diagram of the connection structure of the filter screen and auxiliary mechanism of this utility model;

[0024] Figure 4 This is a schematic diagram of the connection structure of the fixed frame, the side frame, and the filter screen of this utility model;

[0025] Figure 5 This is a schematic diagram of the connection structure between the first guide plate and the first scraper of this utility model;

[0026] Figure 6 This is a schematic diagram of the connection structure between the second guide plate and the second scraper of this utility model;

[0027] Figure 7 This is a schematic diagram of the connection structure of the first screw, the second screw, and the connecting belt of this utility model.

[0028] In the diagram: 1. Filter box; 2. Fixing frame; 3. Frame; 4. Filter screen; 5. Receiving box; 51. Feeding pipe; 6. Auxiliary mechanism; 61. Receiving box; 62. Guide box; 63. First guide plate; 631. Second guide plate; 64. First scraper; 641. Second scraper; 65. First screw; 651. Second screw; 66. Forward and reverse motor; 7. Connecting assembly; 71. Pulley; 72. Connecting belt; 73. Cover; 8. Guide rod. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments of this utility model are described clearly and completely. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0030] This application provides a placental tissue stem cell filtration device, which solves the problem that when the diameter of the outlet port of the feeding pipe is smaller than the size of the filter screen in the filtration device, the cell suspension fed to the filter screen will accumulate in one place on the filter screen, and a lot of excess filtered tissue will accumulate on the filter screen. Over a long period of time, the filter screen pores will be blocked, which will affect the overall filtration efficiency. The device achieves uniform and slow feeding of cell suspension to the filter screen.

[0031] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0032] This utility model discloses a placental tissue stem cell filtration device.

[0033] According to the appendix Figure 1-7 As shown, the system includes a filter box 1, a fixed frame 2 and a receiving box 5 fixedly installed inside the filter box 1, multiple sets of filter screens 4 installed inside the fixed frame 2, and a feed pipe 51 connected to the filter box 1. The inner wall of the fixed frame 2 is fixedly provided with a frame 3 for supporting the filter screens 4. (See attached diagram) Figure 4 From top to bottom, the pore size of the multiple sets of filter screens 4 gradually decreases. Specifically, when filtering placental tissue stem cells, the shredded placental tissue is first placed in a container containing an appropriate amount of digestive enzymes. Enzymatic hydrolysis is carried out under suitable temperature and conditions, causing the cells in the tissue to disperse into single cells or small cell clusters. During the hydrolysis process, gentle stirring or shaking is required to ensure uniform hydrolysis. At the same time, the hydrolysis time and temperature must be strictly controlled to avoid over-hydrolysis and damage to the stem cells. After the hydrolysis is completed, an appropriate amount of buffer solution is added to terminate the hydrolysis reaction. Then, the cell suspension is gently blown with a pipette or other tools to fully disperse the cells. Next, the cell suspension is transported to the inside of the filter box 1 through the pump and the feed pipe 51, so that the cell suspension passes through the multiple sets of filter screens 4 inside the fixed frame 2. The multiple sets of filter screens 4 filter out undigested tissue fragments, thereby obtaining a relatively pure cell suspension. The pure cell suspension is collected in the inside of the receiving box 5, and finally discharged through the discharge pipe connected to the receiving box 5.

[0034] See attached document Figure 2-6An auxiliary mechanism 6 is provided on the upper part of multiple sets of filter screens 4. Through the auxiliary mechanism 6, the cell suspension can be evenly delivered to the filter screens 4, and at the same time, the surface of the filter screens 4 can be cleaned to prevent the filter pores inside the filter screens 4 from clogging. The auxiliary mechanism 6 includes a receiving box 61. A guide box 62 for positioning and moving the receiving box 61 is fixedly provided on the top surface of the fixed frame 2. The receiving box 61 is fixedly connected to one end of the feeding pipe 51. The first guide plate 63 and the first scraper 64 are both fixedly provided on the bottom surface of the receiving box 61. The first screw 65 is rotatably provided inside a set of guide boxes 62. The side wall of the filter box 1 is fixedly provided with a first guide plate 63 for positioning and moving the receiving box 61. The screw 65 rotates bidirectionally, and the reversible motor 66 and another set of guide boxes 62 are both fixedly connected to guide rods 8 for limiting the sliding of the receiving box 61. Specifically, by turning on the reversible motor 66, the reversible motor 66 drives the first screw 65 to rotate in the forward direction first, and then rotate in the reverse direction, so that the receiving box 61 moves back and forth along the top surface of the fixed frame 2. At this time, under the guidance of the first guide plate 63, the cell suspension moves evenly and slowly onto the filter screen 4, thereby achieving full filtration between the cell suspension and the filter screen 4. The first scraper 64 performs a cleaning operation on the surface of the set of filter screens 4 located at the top.

[0035] See attached document Figure 3-6 The second guide plate 631 and the second scraper 641 are slidably disposed on the top surface of another set of filter screens 4. The second screw 651 is rotatably disposed inside the fixed frame 2. The fixed frame 2 is fixedly connected to the guide rod 8. The second screw 651 and the set of guide rods 8 are both located directly above the frame 3. The second guide plate 631 and the second scraper 641 are fixedly connected. One end of the second scraper 641 is threaded onto the outer wall of the second screw 651. The second scraper 641 is slidably disposed on the outer wall of the set of guide rods 8. The second screw 651 drives the second guide plate 631 and the second scraper 641 to move back and forth along the top surface of a set of filter screens 4, so that each set of filter screens 4 located inside the filter box 1 can be guided and unblocked accordingly.

[0036] See attached document Figure 3-4 The lengths of the first guide plate 63, the first scraper 64, the second guide plate 631, and the second scraper 641 are equal to the inner diameter of the frame 3. The bottom ends of the first guide plate 63 and the second guide plate 631 do not contact the surface of the filter screen 4, while the bottom ends of the first scraper 64 and the second scraper 641 contact the surface of the filter screen 4.

[0037] See attached document Figure 2 and attached Figure 7A connecting assembly 7 is provided at one end of the first screw 65 and the second screw 651 extending to the outside of the filter box 1. The connecting assembly 7 is used for linkage between the first screw 65 and the second screw 651. The connecting assembly 7 includes a pulley 71 fixedly disposed at one end of the first screw 65 and the second screw 651 and a connecting belt 72 sleeved between two adjacent sets of pulleys 71. A cover 73 for shielding the connecting belt 72 is fixedly disposed on the side of the filter box 1. That is, when the forward and reverse motor 66 is turned on, the first screw 65 and the second screw 651 rotate synchronously under the connection of multiple sets of connecting belts 72, thereby correspondingly controlling the movement of the first guide plate 63 and the first scraper 64, as well as the second guide plate 631 and the second scraper 641.

[0038] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A placental tissue stem cell filtering device, comprising a filtering box (1), a fixed frame (2) and a receiving box (5) fixedly arranged inside the filtering box (1), a filter screen (4) arranged inside the fixed frame (2), and a feeding pipe (51) connected with the filtering box (1), characterized in that, The upper part of the plurality of groups of filter screens (4) is provided with an auxiliary mechanism (6), and the auxiliary mechanism (6) comprises: A receiving box (61) is fixedly arranged on the top surface of the fixed frame (2), and a guide box (62) for positioning and moving the receiving box (61) is arranged on the top surface of the fixed frame (2). A first guide plate (63) and a first scraper (64) are fixedly arranged on the bottom surface of the receiving box (61). A second guide plate (631) and a second scraper (641) are slidably arranged on the top surface of a group of filter screens (4). A first screw rod (65) is rotatably arranged in the guide box (62), and a forward-reverse motor (66) for bidirectional rotation of the first screw rod (65) is fixedly arranged on the side wall of the filter box (1). A second screw rod (651) is rotatably arranged in the fixed frame (2), and the second guide plate (631) and the second scraper (641) are driven to reciprocate along the top surface of a group of filter screens (4) by the second screw rod (651).

2. The placental tissue stem cell filtration device of claim 1, wherein: The inner wall of the fixed frame (2) is fixedly provided with a frame (3) for supporting the filter screen (4), and the lengths of the first guide plate (63) and the first scraper (64) and the second guide plate (631) and the second scraper (641) are equal to the inner diameter of the frame (3).

3. The placental tissue stem cell filtration device of claim 2, wherein: The bottom ends of the first guide plate (63) and the second guide plate (631) are not in contact with the surface of the filter screen (4), and the bottom ends of the first scraper (64) and the second scraper (641) are in contact with the surface of the filter screen (4).

4. The placental tissue stem cell filtration device of claim 1, wherein: The first screw rod (65) and the second screw rod (651) extend to one end outside the filter box (1) and are provided with a connecting assembly (7), and the connecting assembly (7) is used for linkage between the first screw rod (65) and the second screw rod (651).

5. The placental tissue stem cell filtration device of claim 4, wherein: The connecting assembly (7) comprises a belt pulley (71) fixedly arranged at one end of the first screw rod (65) and the second screw rod (651), and a connecting belt (72) sleeved between adjacent two groups of belt pulleys (71).

6. The placental tissue stem cell filtration device of claim 5, wherein: A cover (73) for shielding the connecting belt (72) is fixedly arranged on the side surface of the filter box (1).

7. The placental tissue stem cell filtration device of claim 1, wherein: The guide box (62) and the interior of the fixed frame (2) are fixedly connected with a guide rod (8).

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