Cell culture device convenient for sampling

Abstract

The utility model relates to the technical field of cell culture, and discloses a cell culture device convenient for sampling, which comprises a bacteria culture dish, the top of the bacteria culture dish is provided with a sealing cover, and the upper surface of the sealing cover is uniformly provided with a plurality of clamp cavities. By arranging the sampling pipe and the valve plate, when the sampling pipe is pressed, the bottom end of the sampling pipe can extrude the valve plate, the valve plate can move downwards by a certain distance after being extruded, when the valve plate moves downwards to the middle of the positioning ring, an annular gap can be generated between the valve plate and the positioning ring, at the moment, the pull rod is pulled upwards, and the valve plate can be pulled out. The drawing rod can drive the drawing plug to slide towards the upper portion of the sampling pipe for sampling, after sampling is completed, when the sampling pipe moves towards the upper portion of the clamping cavity, the valve plate can move upwards to reset under the action of no pressure to plug the opened valve port again, and cells can be rapidly sampled conveniently. And the cells can be prevented from being polluted by external air in the sampling process.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a cell culture device that facilitates sampling. Background Technology

[0002] Cell culture is a method that simulates the in vivo environment (sterile, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structure and function. It requires certain conditions, and the cells are generally placed in a culture dish, which is then placed in a cell culture incubator for cultivation.

[0003] Existing cell culture dishes typically require unscrewing the sealed cap before sampling the cells, which is cumbersome. During sampling, the cells are exposed to air, allowing airborne bacteria to enter and contaminate the cells, thus affecting subsequent cell culture outcomes. Therefore, those skilled in the art have provided a convenient cell culture device for sampling to address the problems mentioned in the background section. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies, such as cumbersome sampling and the potential for contamination of cells in the culture dish during sampling, which can affect subsequent cell culture results. Therefore, this invention proposes a cell culture device that facilitates sampling.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cell culture device for convenient sampling, comprising a bacterial culture dish, the top of which is provided with a sealing cap, and multiple clamping cavities are evenly distributed on the upper surface of the sealing cap. Sampling tubes are clamped into the inner sides of each clamping cavity. A valve cavity is fixedly connected to the middle of the bottom end of each clamping cavity. A leaking plate is fixedly connected to the bottom end of the inner side of the valve cavity. A positioning ring is fixedly connected to the outer edge of the top of the leaking plate. A sliding rod is slidably connected to the middle of the leaking plate. A valve plate is fixedly connected to the top of the sliding rod. The valve plate is slidably connected to the positioning ring. A return spring is provided in the middle between the valve plate and the leaking plate. The return spring is located outside the sliding rod. A limiting plate is provided above the valve plate. The outer side of the limiting plate is fixedly connected to the upper side of the inner side of the valve cavity.

[0006] With the above technical solution, during sampling, pressing the sampling tube causes the column tube fixedly connected to the bottom center of the sampling tube to pass through the limiting plate and squeeze the valve plate. After being squeezed, the valve plate moves downward a certain distance under the restriction of the sliding rod, causing the valve plate to separate from the top of the positioning ring. When the valve plate moves down to the middle of the positioning ring, an annular gap is created between the valve plate and the positioning ring. At this time, the suction force generated by the sampling tube will cause the cells in the bacterial culture dish to pass through the leak plate and the annular gap between the valve plate and the positioning ring and enter the column tube and the sampling tube fixedly connected to the top of the column tube. After sampling, when the sampling tube moves upward to the clamp cavity, the valve plate will move upward to reset under no pressure and reseal the opened valve port to prevent external air from entering the bacterial culture dish. This facilitates rapid cell sampling and helps prevent cell contamination by external air during the sampling process.

[0007] Furthermore, a pumping plug is provided at the bottom of the inner side of the sampling tube, and a pull rod is fixedly connected to the middle of the top of the pumping plug. A fixed plate is slidably connected to the top of the pull rod, and the lower part of the fixed plate is rotatably connected to the upper part of the inner side of the clamp cavity through a thread.

[0008] With the above technical solution, before sampling, rotating the fixed plate separates the thread on the lower outer side of the fixed plate from the thread on the upper inner side of the clamp cavity, thereby separating the fixed plate from the clamp cavity. At this time, pulling the fixed plate upward will cause the fixed plate to slide the pull rod of the middle clamp upward, making it easy to fix the sampling tube into the clamp cavity when not in use.

[0009] Furthermore, a test tube placement cavity is provided on the upper surface of the sealing cap at an oblique angle between the four clamp cavities, and a docking cavity is provided on the outer side of the test tube placement cavity. The docking cavity is opened on the upper surface of the inner side of the bacterial culture dish.

[0010] With the above technical solution, the sampling tube can be placed in the tube placement cavity during sampling, which facilitates the fixation of the tube during sampling. The tube placement cavity and the docking cavity can be interlocked, which helps to improve the stability of the clamp between the sealing cap and the bacterial culture dish.

[0011] Furthermore, a bacterial culture tank is provided on the outside of the clamp cavity. The bacterial culture tank is located inside the bacterial culture dish. A sealing ring is clamped above the inside of the bacterial culture tank. The top of the sealing ring is fixedly connected to the bottom of the test tube placement cavity.

[0012] With the above technical solution, the sealing ring, when the sealing cap and the bacterial culture dish are engaged, will engage with the upper part of the inner side of the bacterial culture tank, which helps to improve the sealing performance of the sealing cap engagement.

[0013] Furthermore, a docking ring is fixedly connected to the outer edge of the upper surface of the bacterial culture dish, and a docking groove is provided on the outer side of the lower surface of the sealing cap, and the docking ring can be engaged with the docking groove.

[0014] The above technical solution, with its designed docking ring and docking groove, enhances the stability of the connection between the bacterial culture dish and the sealing cap when they are engaged.

[0015] Furthermore, a limiting ring is fixedly connected to the upper outer side of the pull rod, and a limiting column cavity is opened in the middle of the inner side of the fixed plate. The fixed plate is slidably connected to the upper outer side of the pull rod through the limiting column cavity and the limiting ring.

[0016] With the above technical solution, when the fixed plate rotates, the limiting cylinder cavity in its middle will rotate around the limiting ring fixedly connected to the upper outer side of the pull rod to one end. When the fixed plate moves up, the fixed plate moves up along the pull rod under the restriction of the limiting cylinder cavity. When the top of the inner side of the limiting cylinder cavity slides and locks to the bottom of the limiting ring, the fixed plate stops sliding up, which makes it easier to limit the trajectory of the fixed plate sliding up and down.

[0017] This utility model has the following beneficial effects:

[0018] 1. In this utility model, a sampling tube, valve plate, slide rod, positioning ring, pull rod, delivery plug, and leak plate are configured. When the sampling tube is pressed, the column tube fixedly connected to the bottom middle of the sampling tube will pass through the limiting plate and squeeze the valve plate. After being squeezed, the valve plate will move downward a certain distance under the restriction of the slide rod, so that the valve plate separates from the top of the positioning ring. When the valve plate moves down to the middle of the positioning ring, an annular gap will be generated between the valve plate and the positioning ring. At this time, the pull rod is pulled upward, and the pull rod will drive the delivery plug to slide upward to the sampling tube. The suction force generated during the upward sliding of the delivery plug will cause the cells in the bacterial culture dish to pass through the leak plate and the annular gap between the valve plate and the positioning ring and enter the column tube and the sampling tube fixedly connected to the top of the column tube. After sampling is completed, when the sampling tube moves upward to the clamp cavity, the valve plate will move upward to reset under no pressure and reseal the opened valve port, which facilitates rapid cell sampling and helps to prevent the cells from being contaminated by external air during the sampling process. Attached Figure Description

[0019] Figure 1 This is a perspective view of a cell culture device for convenient sampling proposed in this utility model;

[0020] Figure 2 A three-dimensional view showing the disassembled bacterial culture dish and sampling tube of a cell culture device for convenient sampling proposed in this utility model.

[0021] Figure 3A schematic diagram of the connection structure between the bacterial culture dish and the sealing cap of a cell culture device for convenient sampling proposed in this utility model.

[0022] Figure 4 This is an enlarged structural diagram of section A of a cell culture device for convenient sampling proposed in this utility model.

[0023] Legend:

[0024] 1. Bacterial culture dish; 2. Sealing cap; 3. Test tube placement cavity; 4. Fixing plate; 5. Clamp cavity; 6. Bacterial culture tank; 7. Docking cavity; 8. Sampling tube; 9. Pull rod; 10. Pumping plug; 11. Valve cavity; 12. Limiting plate; 13. Valve plate; 14. Positioning ring; 15. Slot plate; 16. Slide rod; 17. Return spring. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Reference Figure 1-4 This utility model provides an embodiment of a cell culture device for convenient sampling, comprising a bacterial culture dish 1, a sealing cap 2 on the top of the bacterial culture dish 1, a plurality of clamping cavities 5 evenly distributed on the upper surface of the sealing cap 2, and sampling tubes 8 clamped inside each of the clamping cavities 5. A valve cavity 11 is fixedly connected to the middle of the bottom end of the clamping cavity 5, and a leak plate 15 is fixedly connected to the bottom end of the inner side of the valve cavity 11. A positioning ring 14 is fixedly connected to the outer edge of the top of the leak plate 15. A sliding rod 16 is slidably connected to the middle of the leak plate 15, and a valve plate 13 is fixedly connected to the top of the sliding rod 16. The valve plate 13 is slidably connected to the positioning ring 14, and the valve plate 13 and the leak plate 15 are connected in a sliding manner. A return spring 17 is provided in the middle of the valve plate 13. The return spring 17 is located outside the slide rod 16. A limiting plate 12 is provided above the valve plate 13. The outer side of the limiting plate 12 is fixedly connected to the upper side of the inner side of the valve cavity 11. A pumping plug 10 is provided at the bottom of the inner side of the sampling tube 8. A pull rod 9 is fixedly connected to the middle of the top of the pumping plug 10. A fixed plate 4 is slidably connected to the top of the pull rod 9. The lower part of the fixed plate 4 is rotatably connected to the upper side of the inner side of the clamp cavity 5 through a thread. A limiting ring is fixedly connected to the upper side of the outer side of the pull rod 9. A limiting column cavity is opened in the middle of the inner side of the fixed plate 4. The fixed plate 4 is slidably connected to the upper side of the outer side of the pull rod 9 through the limiting column cavity and the limiting ring.

[0027] Preferably, a test tube placement cavity 3 is provided on the upper surface of the sealing cap 2 at an oblique angle between the four clamping cavities 5. A docking cavity 7 is provided on the outer side of the test tube placement cavity 3. The docking cavity 7 is opened on the upper surface of the inner side of the bacterial culture dish 1. A bacterial culture tank 6 is provided on the outer side of the clamping cavity 5. The bacterial culture tank 6 is located on the inner side of the bacterial culture dish 1. A sealing ring is clamped on the upper side of the inner side of the bacterial culture tank 6. The top of the sealing ring is fixedly connected to the bottom of the test tube placement cavity 3. A docking ring is fixedly connected to the outer edge of the upper surface of the bacterial culture dish 1. A docking groove is opened on the outer side of the lower surface of the sealing cap 2. The docking ring can be engaged with the docking groove.

[0028] Working principle: During sampling, the sampling tube is placed in the tube placement cavity 3. Rotating the fixing plate 4 separates the thread on the lower outer side of the fixing plate 4 from the thread on the upper inner side of the clamp cavity 5, thus separating the fixing plate 4 from the clamp cavity 5. At this time, pulling the fixing plate 4 upward will cause the pull rod 9 of the middle clamp to slide upward a certain distance. Then, pressing the sampling tube 8 will cause the column tube fixedly connected to the bottom middle of the sampling tube 8 to pass through the limiting plate 12 and squeeze the valve plate 13. After being squeezed, the valve plate 13 will move downward a certain distance under the restriction of the sliding rod 16, so that the top part of the valve plate 13 and the positioning ring 14 are separated. When the valve plate 13 moves down to the middle of the positioning ring 14, an annular gap will be generated between the valve plate 13 and the positioning ring 14. At this time, pull the pull rod 9 upward, and the pull rod 9 will drive the pump plug 10 to slide upward to the sampling tube 8. The pumping force generated during the upward sliding of the pump plug 10 will cause the cells in the bacterial culture dish 1 to pass through the leak plate 15 and the annular gap between the valve plate 13 and the positioning ring 14 and enter the column tube and the sampling tube 8 fixedly connected to the top of the column tube. After the sampling is completed, when the sampling tube 8 moves upward to the clamp cavity 5, the valve plate 13 will move upward to reset under no pressure and reseal the opened valve port.

[0029] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A cell culture device for convenient sampling comprising a bacterial culture dish (1) characterized in that: The top of the bacterial culture dish (1) is provided with a sealing cover (2), the upper surface of the sealing cover (2) is uniformly provided with a plurality of clamp cavities (5), the inner side of the plurality of clamp cavities (5) is clamped with a sampling tube (8), the middle of the bottom end of the clamp cavity (5) is fixedly connected with a valve cavity (11), the inner side of the bottom end of the valve cavity (11) is fixedly connected with a sieve plate (15), the outer edge of the top end of the sieve plate (15) is fixedly connected with a positioning ring (14), the middle of the sieve plate (15) is slidably connected with a slide rod (16), the top of the slide rod (16) is fixedly connected with a valve plate (13), the valve plate (13) is slidably connected with the positioning ring (14), the middle between the valve plate (13) and the sieve plate (15) is provided with a return spring (17), the return spring (17) is located on the outer side of the slide rod (16), the upper side of the valve plate (13) is provided with a limiting plate (12), the outer side of the limiting plate (12) is fixedly connected with the upper side of the inner side of the valve cavity (11).

2. The cell culture device of claim 1, wherein: The bottom end of the inner side of the sampling tube (8) is provided with a pumping plug (10), the middle of the top end of the pumping plug (10) is fixedly connected with a pulling rod (9), the top of the pulling rod (9) is slidably connected with a fixed disc (4), the lower side of the fixed disc (4) is threadedly rotatably connected with the upper side of the inner side of the clamp cavity (5).

3. The cell culture device of claim 1, wherein: The obliquely opposite corners between the four clamp cavities (5) on the upper surface of the sealing cover (2) are provided with a test tube placing cavity (3), the outer side of the test tube placing cavity (3) is provided with a butt joint clamping cavity (7), the butt joint clamping cavity (7) is formed on the upper surface of the inner side of the bacterial culture dish (1).

4. The cell culture device of claim 1, wherein: The outer side of the clamp cavity (5) is provided with a bacterial culture tank (6), the bacterial culture tank (6) is located on the inner side of the bacterial culture dish (1), the inner side of the bacterial culture tank (6) is clamped with a sealing ring on the upper side, and the top of the sealing ring is fixedly connected with the bottom of the test tube placing cavity (3).

5. The cell culture device of claim 1, wherein: The outer edge of the upper surface of the bacterial culture dish (1) is fixedly connected with a butt joint clamping ring, the outer side of the lower surface of the sealing cover (2) is provided with a butt joint clamping groove, and the butt joint clamping ring and the butt joint clamping groove are clamped with each other.

6. The cell culture device of claim 2, wherein: The upper side of the outer side of the pulling rod (9) is fixedly connected with a limiting ring, the middle of the inner side of the fixed disc (4) is provided with a limiting column cavity, and the fixed disc (4) is slidably connected with the upper side of the outer side of the pulling rod (9) through the limiting column cavity and the limiting ring.

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