Liquid suction and drainage device with pressure monitoring function

Abstract

The liquid suction and drainage device with the pressure monitoring function comprises a pipette connector, the pipette connector comprises a connecting body, the connecting body is internally provided with a cavity part, the lower end of the cavity part is open, the lower end of the cavity part is provided with a bottom cover, the lower end of the bottom cover is provided with a connecting nozzle, and the connecting nozzle is used for being connected with a pipette in a sleeved mode. A negative pressure air inlet and a positive pressure air inlet which are communicated with the cavity part are formed in the upper end of the connecting main body, an air pressure monitoring hole is formed in the side wall of the cavity part, and a pressure gauge is arranged at the air pressure monitoring hole, so that the problem that the reagent suction amount of a pipette is inaccurate due to inaccurate pressure monitoring in an automatic pipetting mechanism is solved.

Description

Technical Field

[0001] This utility model relates to the field of glass slide preparation, and in particular to a liquid suction and discharge device with pressure monitoring. Background Technology

[0002] In liquid-based slide preparation technology, cells or tissues are collected using a brush or other collection tools, and the samples are stored in a sampling bottle containing a preservation solution. The bottle is then sealed and transported to the slide preparation station, where necessary reagents are added to the cell samples. The samples are then transferred to the slide preparation chamber of a centrifuge for centrifugation and are made into glass slides.

[0003] In automated slide preparation equipment, due to the large workload and high precision requirements, traditional manual operation is no longer sufficient for quantitative reagent addition and sample transfer. Therefore, automated pipettes are typically used for reagent addition and sample transfer. Traditional pipettes usually use negative pressure for liquid aspiration. After aspiration, the negative pressure valve switches to a closed state, maintaining negative pressure in the pipette. When liquid needs to be expelled, the pipette switches to positive pressure to expel the liquid from the pipette. Because quantitative reagent addition is required, a negative pressure gauge is generally installed in the negative pressure circuit to monitor the pressure. Based on the pressure, the liquid level in the end of the pipette can be calculated, thus determining the amount of reagent aspirated.

[0004] Traditional negative pressure monitoring meters are installed in the gas path system, which is far from the end pipette. Because there are many components in the gas path, there may be slight air leakage at each joint. This results in a difference between the actual negative pressure at the pipette and the pressure at the installation location of the negative pressure monitoring meter, leading to inaccurate monitoring and a mismatch between the amount of reagent drawn and the set amount. Utility Model Content

[0005] This invention provides a liquid aspiration and dispensing device with pressure monitoring, which solves the problem of inaccurate reagent aspiration volume caused by inaccurate pressure monitoring in automatic liquid transfer mechanisms.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is: a liquid suction and discharge device with pressure monitoring, including a pipette connector, the pipette connector including a connecting body, a cavity part provided inside the connecting body, an opening at the lower end of the cavity part, a bottom cover provided at the lower end of the cavity part, a connecting nozzle provided at the lower end of the bottom cover, the connecting nozzle being used to connect with a pipette, a negative pressure air inlet and a positive pressure air inlet communicating with the cavity part at the upper end of the connecting body, a pressure monitoring hole provided on the side wall of the cavity part, and a pressure gauge provided at the pressure monitoring hole.

[0007] In a preferred embodiment, the negative pressure air inlet and the positive pressure air inlet are provided with a convex ring structure at one end near the cavity. The convex ring structure of the negative pressure air inlet and the convex ring structure of the positive pressure air inlet are respectively fitted with a first porous cover and a second porous cover. The outer side of the first porous cover is fitted with a porous filter cover, and the inner side of the second porous cover is provided with a porous filter sheet.

[0008] In a preferred embodiment, a separating rib is provided between the convex ring structure of the negative pressure air inlet and the convex ring structure of the positive pressure air inlet inside the cavity.

[0009] In a preferred embodiment, the connector is provided with multiple cylindrical sleeve portions arranged in a stepped manner, which are used to connect with the pipette.

[0010] In a preferred embodiment, a vertically movable lower extension rod connecting plate is also included. The pipette connector is located on the lower extension rod connecting plate. A flange is provided on the outer edge of the upper end of the pipette. A pipette unloading rack is provided on one side of the pipette connector. A lever is provided at the lower end of the unloading rack, and the end of the lever is attached to the outer wall of the connecting nozzle.

[0011] In a preferred embodiment, a third linear motion mechanism is also included. The third linear motion mechanism includes a base frame, on which a lifting frame plate that can move up and down is provided. A lower extension rod is provided on the lifting frame plate, and the lower end of the lower extension rod is connected to a lower extension rod connecting plate. A lower extension rod guide seat is provided on the base frame, and the lower extension rod guide seat is slidably sleeved with the lower extension rod. A lifting plate guide rod is provided at the upper end of the pipe unloading frame, and a guide rod guide seat is also provided on the base frame. The lifting plate guide rod is slidably sleeved with the guide rod guide seat. A spring balancer is provided at the upper end of the base frame, and the spring balancer suspends the upper end of the lifting plate guide rod.

[0012] In a preferred embodiment, the lower end of the pipette rack has a hollow section with a hinge shaft at the hollow section. One end of the lever is hinged to the hinge shaft, and the lever has a first countersunk hole. A baffle is provided on the side of the hinge shaft away from the pipette connector, and a second countersunk hole is provided inside the baffle. A spring is also provided, with one end of the spring inserted into the first countersunk hole and abutting against the bottom of the first countersunk hole, and the other end of the spring inserted into the second countersunk hole and abutting against the bottom of the second countersunk hole.

[0013] The beneficial effects of this utility model are as follows: An electronic pressure monitoring gauge is connected to the side wall of the cavity of the connecting body, and the monitoring position is closer to the pipette, so the monitored value is closer to the actual value; the cavity of the connecting body has a large volume, so if the air pressure monitoring is inaccurate or the pressure fluctuates, causing the amount of reagent or sample drawn to exceed the set value, the cavity can act as a buffer to accommodate a large amount of liquid, avoiding the liquid being sucked into the gas path system and causing contamination of the gas path system; the positive pressure gas path and the negative pressure gas path are set separately, so there is no need to wait for the negative pressure gas path to fill the positive pressure, the liquid discharge action can be faster, and the gas path design is simpler; a detachable pipette is connected through the pipette connector, and the pipette connector is provided with cylindrical sleeves of various diameters to accommodate various pipette specifications; the lever can swing and always stays close to the outer wall of the connecting nozzle. After the liquid is pipetted, the pipette is automatically removed by the lever and replaced with a new clean pipette, avoiding cross-contamination of the sample. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0015] Figure 1 This is a schematic diagram of a pipette and its Cartesian three-dimensional movement mechanism.

[0016] Figure 2 This is a schematic diagram of the up-and-down movement mechanism of a pipette.

[0017] Figure 3 This is a partial view of the pipette connector.

[0018] Figure 4 This is a diagram of the external structure of a pipette connector.

[0019] Figure 5 This is a cross-sectional view of the connector.

[0020] Figure 6 This is a diagram showing the non-working position of the paddle shifter.

[0021] Figure 7 This is a schematic diagram of the lowest working position of the paddle shifter.

[0022] Figure 8 This is a cross-sectional view of a pipette connector.

[0023] Figure 9 This is an exploded view of a pipette connector.

[0024] Figure 10 This is a diagram showing the installation location of the pressure gauge.

[0025] Figure 11 It is a diagram showing the internal structure of the connected main body.

[0026] In the figure: First linear movement mechanism 1; transverse frame 101; base plate 102; first guide rail slider device 103; first drive motor 104; first lead screw 105; first lead screw nut 106; Second linear movement mechanism 2; base frame 201; cantilever frame 202; second guide rail slider device 203; second drive motor 204; synchronous belt mechanism 205; synchronous belt clamping block 206; Third linear movement mechanism 3; lifting frame plate 301; third drive motor 302; second lead screw 303; second lead screw nut 304; third guide rail slider device 305; lower extension rod 4; lower extension rod connecting plate 401; lower extension rod guide seat 402; positioning hole 403; locking screw 404; pipette connector 5; connecting nozzle 501; Cylindrical sleeve part 502; Negative pressure air inlet 503; Positioning protrusion 504; Annular groove part 505; Notch part 506; Air connector 507; Positive pressure air inlet 508; Connecting body 509; Cavity part 510; Bottom cover 511; Air pressure monitoring hole 512; First porous cover 513; Second porous cover 514; Protruding ring structure 515; Filter cover 516; Filter sheet 517; Separating rib 518; Unloading pipe rack 6; Paddle 601; Lifting plate guide rod 602; Guide rod guide seat 603; Slot part 604; Hollowed-out part 605; Hinge shaft 606; First countersunk hole 607; Spring 608; Baffle 609; Second countersunk hole 610; Pipette 7; Flange edge 701; Spring balancer 8; Pressure gauge 9. Detailed Implementation

[0027] like Figure 1-11 In the present invention, a pressure monitoring liquid aspiration and dispensing device includes a pipette connector 5, which includes a connecting body 509, a cavity 510 inside the connecting body 509, an opening at the lower end of the cavity 510, a bottom cover 511 at the lower end of the cavity 510, a connecting nozzle 501 at the lower end of the bottom cover 511, the connecting nozzle 501 being used to connect with a pipette 7, a negative pressure air inlet 503 and a positive pressure air inlet 508 communicating with the cavity 510 at the upper end of the connecting body 509, a pressure monitoring hole 512 on the side wall of the cavity 510, and a pressure gauge 9 at the pressure monitoring hole 512.

[0028] Pressure gauge 9 can be fixed on the lower extension rod connecting plate and connected to the air pressure monitoring hole 512 through a short air tube.

[0029] The negative pressure air inlet 503 is connected to the negative pressure air path of the air path system, and the positive pressure air inlet 508 is connected to the positive pressure air path of the air path system.

[0030] Pressure gauge 9 is an electronic pressure gauge that can monitor the positive and negative pressure inside the cavity 510 and feed the signal back to the air circuit system to dynamically adjust the air pressure in the cavity 510 to keep it stable.

[0031] During liquid aspiration, pipette 7 is inserted into the reagent bottle, the negative pressure air path is connected, and the positive pressure air path is closed. Pressure gauge 9 monitors the negative pressure to ensure that pipette 7 can aspirate the liquid to the set height. During liquid drainage, the negative pressure air path is closed, and the positive pressure air path is connected. Pressure gauge 9 monitors the positive pressure to confirm that the positive pressure air path is not blocked, that the positive pressure can reach the set value and be maintained for a period of time, and that the liquid aspirated in pipette 7 is completely drained.

[0032] In a preferred embodiment, the negative pressure air inlet 503 and the positive pressure air inlet 508 are provided with a convex ring structure 515 at one end near the cavity 510. The convex ring structure 515 of the negative pressure air inlet 503 and the convex ring structure 515 of the positive pressure air inlet 508 are respectively fitted with a first porous cover 513 and a second porous cover 514. A porous filter cover 516 is fitted on the outside of the first porous cover 513, and a porous filter sheet 517 is provided inside the second porous cover 514.

[0033] The first porous cover 513 and the second porous cover 514 serve as load-bearing support structures. The filter cover 516 and the filter sheet 517 are made of porous sponge-like material, which can prevent pollutants in the airflow from entering the positive and negative pressure air paths.

[0034] In a preferred embodiment, a separating rib 518 is provided between the convex ring structure 515 of the negative pressure air inlet 503 and the convex ring structure 515 of the positive pressure air inlet 508 in the cavity 510.

[0035] When blowing air under positive pressure, avoid excessive lateral airflow to blow contaminants attached to the filter cover 516 into the reagent bottle or sample bottle.

[0036] In a preferred embodiment, the connector 501 is provided with a plurality of cylindrical sleeve portions 502 arranged in a stepped manner, the cylindrical sleeve portions 502 being used to sleeve with the pipette 7.

[0037] The diameter of the lower cylindrical sleeve portion 502 is slightly smaller than that of the upper cylindrical sleeve portion 502, so the connecting nozzle 501 can be fitted with various pipettes 7 of different diameters.

[0038] In a preferred embodiment, a vertically movable lower extension rod connecting plate 401 is also included. The pipette connector 5 is disposed on the lower extension rod connecting plate 401. A flange edge 701 is provided on the upper outer edge of the pipette 7. A pipette unloading rack 6 is provided on one side of the pipette connector 5. A lever 601 is provided at the lower end of the pipette unloading rack 6. The end of the lever 601 is attached to the outer wall of the connecting nozzle 501.

[0039] The pipette 7 adopts a customized structure with an outwardly protruding flange 701 on the outer edge of the upper port. When the lower extension rod connecting plate 401 moves the pipette connector 5 upward, the paddle 601 has a slot 604. The slot 604 abuts against the flange 701 and scrapes the pipette 7 off.

[0040] In a preferred embodiment, a third linear motion mechanism 3 is also included. The third linear motion mechanism 3 includes a base frame 201, on which a vertically movable lifting frame plate 301 is provided. A lower extension rod 4 is provided on the lifting frame plate 301, and the lower end of the lower extension rod 4 is connected to a lower extension rod connecting plate 401. A lower extension rod guide seat 402 is provided on the base frame 201, and the lower extension rod guide seat 402 is slidably sleeved with the lower extension rod 4. A lifting plate guide rod 602 is provided at the upper end of the pipe unloading frame 6. A guide rod guide seat 603 is also provided on the base frame 201, and the lifting plate guide rod 602 is slidably sleeved with the guide rod guide seat 603. A spring balancer 8 is provided at the upper end of the base frame 201, and the spring balancer 8 suspends the upper end of the lifting plate guide rod 602.

[0041] When the pipette connector 5 moves downward, its lower end abuts against the lever 601, causing the pipette rack 6 to descend. The connector 501 is inserted into the pipette 7 on the lower pipette rack. Since the pipette 7 is made of plastic, it has a certain degree of elasticity. The beveled structure at the lower end of the cylindrical sleeve 502 is inserted into the pipette 7 first, and the pipette opening expands slightly and fits onto the cylindrical sleeve 502, which can form a seal and has a certain clamping force.

[0042] Subsequently, the lifting platform 301 moves upward, causing the pipette 7 to detach from the pipe rack. The spring balancer 8 adjusts the tension to be slightly greater than the total weight of the unloading rack 6 and related structures. Under the action of the spring balancer 8, the unloading rack 6 moves upward and resets along with the pipette connector 5. When the upper end of the unloading rack 6 is stopped by the guide rod guide seat 603, it no longer moves upward. At this time, the pipette connector 5 continues to move upward, and the lever 601 can scrape off the pipette 7.

[0043] In a preferred embodiment, the lower end of the tube unloading rack 6 is provided with a hollow portion 605, and a hinge shaft 606 is provided at the hollow portion 605. One end of the lever 601 is hinged to the hinge shaft 606. The lever 601 is provided with a first countersunk hole 607. A baffle 609 is provided on the side of the hinge shaft 606 away from the pipette connector 5. A second countersunk hole 610 is provided in the baffle 609. A spring 608 is also provided. One end of the spring 608 is inserted into the first countersunk hole 607 and abuts against the bottom end of the first countersunk hole 607. The other end of the spring 608 is inserted into the second countersunk hole 610 and abuts against the bottom end of the second countersunk hole 610.

[0044] Under the elastic force of spring 608, the end of the paddle 601 is always tightly pressed against the outer wall of the connector 501. No matter what diameter pipette 7 is fitted onto the connector 501, the slot 604 can abut against the cylindrical sleeve 502 of the next level. When the connector 501 moves upward relative to the paddle 601, the pipette 7 is scraped off.

[0045] The lower extension rod connecting plate 401 is provided with a positioning hole 403, and the upper end of the pipette connector 5 is provided with a positioning protrusion 504. The positioning protrusion 504 is sleeved with the positioning hole 403. The outer wall of the positioning protrusion 504 is provided with an annular groove 505. The side wall of the positioning hole 403 is provided with a threaded locking screw 404. The locking screw 404 is inserted into the annular groove 505 and presses against the positioning protrusion 504.

[0046] The positioning protrusion 504 has a notch 506 at the negative pressure air inlet 503 and the positive pressure air inlet 508, and an air connector 507 is provided at the negative pressure air inlet 503, the positive pressure air inlet 508 and the air pressure monitoring hole 512.

[0047] The air connector 507 is used to connect to an external negative pressure and positive pressure switching air pipe.

[0048] It also includes a third linear motion mechanism 3, which includes a base frame 201. The base frame 201 is provided with a second lead screw 303 and a third guide rail slider device 305. A second lead screw nut 304 is provided on one side of the lifting frame plate 301. The second lead screw nut 304 is sleeved with the second lead screw 303. One end of the second lead screw nut 304 is slidably connected to the base frame 201 through the third guide rail slider device 305. A third drive motor 302 is also provided on the base frame 201. The shaft end of the third drive motor 302 is connected to the second lead screw 303.

[0049] The system also includes a first linear moving mechanism 1 and a second linear moving mechanism 2. The first linear moving mechanism 1 includes a transverse frame 101 and a base plate 102. A first guide rail slider device 103 is provided on the base plate 102. The transverse frame 101 and the base plate 102 are slidably connected by the first guide rail slider device 103. A first lead screw 105 is provided on the base plate 102. A first drive motor 104 is provided at the shaft end of the first lead screw 105. A first lead screw nut 106 is also provided on the side of the transverse frame 101 near the base plate 102. 106 is sleeved with the first lead screw 105. The second linear movement mechanism 2 includes a cantilever 202. The cantilever 202 is provided with a second guide rail slider 203. The base frame 201 is slidably connected to the cantilever 202 through the second guide rail slider 203. The cantilever 202 is provided with a second drive motor 204 and a synchronous belt mechanism 205. The base frame 201 is provided with a synchronous belt clamping block 206. The second drive motor 204 drives the base frame 201 to move through the synchronous belt mechanism 205.

[0050] The first linear movement mechanism 1 is installed on the frame of the slide preparation machine. The first linear movement mechanism 1, the second linear movement mechanism 2 and the third linear movement mechanism 3 constitute a Cartesian movement mechanism, which drives the pipette connector 5 to move freely in three orthogonal directions, ensuring that liquid can be aspirated, pipetted and added at any position in the space of the slide preparation equipment.

[0051] The above embodiments are merely preferred technical solutions of this utility model and should not be considered as limitations on this utility model. The protection scope of this utility model should be the technical solution described in the claims, including equivalent substitutions of the technical features described in the claims. That is, equivalent substitutions and improvements within this scope are also within the protection scope of this utility model.

Claims

1. A suction and drainage device with pressure monitoring, characterized by: The utility model provides a pipette connector (5) comprising a connecting body (509) having a cavity (510) formed therein, the cavity (510) having an open lower end, the cavity (510) having a bottom cover (511) formed at the lower end, the bottom cover (511) having a connecting nozzle (501) formed at the lower end, the connecting nozzle (501) being configured to be fitted with a pipette (7), the connecting body (509) having a negative pressure air inlet hole (503) and a positive pressure air inlet hole (508) formed at the upper end and communicating with the cavity (510), the cavity (510) having a pressure monitoring hole (512) formed in the side wall, and the pressure monitoring hole (512) having a pressure gauge (9) formed therein.

2. The liquid suction device with pressure monitoring according to claim 1, characterized in that: The negative pressure air inlet hole (503) and the positive pressure air inlet hole (508) have a convex ring structure (515) formed at one end close to the cavity (510), the convex ring structure (515) of the negative pressure air inlet hole (503) and the convex ring structure (515) of the positive pressure air inlet hole (508) having a first porous cover (513) and a second porous cover (514) fitted thereon, respectively, the first porous cover (513) having a porous filter cover (516) fitted thereon, and the second porous cover (514) having a porous filter sheet (517) formed therein.

3. The liquid suction device with pressure monitoring according to claim 2, characterized in that: The cavity (510) has a partition rib (518) formed between the convex ring structure (515) of the negative pressure air inlet hole (503) and the convex ring structure (515) of the positive pressure air inlet hole (508).

4. The liquid suction device with pressure monitoring according to claim 1, characterized in that: The connecting nozzle (501) has a plurality of cylindrical sleeve portions (502) arranged in a stepped manner, and the cylindrical sleeve portions (502) are configured to be fitted with the pipette (7).

5. The apparatus for monitoring the pressure of claim 1, wherein: The utility model also comprises a vertically movable lower extension rod connecting plate (401), the pipette connector (5) being arranged on the lower extension rod connecting plate (401), the pipette (7) having a flange (701) formed at the outer edge of the upper end, one side of the pipette connector (5) having a tube dismounting rack (6), the tube dismounting rack (6) having a push piece (601) formed at the lower end, and the push piece (601) abutting against the outer wall of the connecting nozzle (501).

6. The liquid suction device with pressure monitoring according to claim 5, characterized in that: The utility model also comprises a third linear movement mechanism (3), the third linear movement mechanism (3) comprising a base frame (201), the base frame (201) having a vertically movable lifting frame plate (301) arranged thereon, the lifting frame plate (301) having a lower extension rod (4) arranged thereon, the lower extension rod (4) being connected to the lower extension rod connecting plate (401), the base frame (201) having a lower extension rod guide seat (402) arranged thereon, the lower extension rod guide seat (402) being slidably fitted with the lower extension rod (4), the tube dismounting rack (6) having a lifting plate guide rod (602) arranged at the upper end, the base frame (201) further having a guide rod guide seat (603) arranged at the upper end, the lifting plate guide rod (602) being slidably fitted with the guide rod guide seat (603), and the base frame (201) having a spring balancer (8) arranged at the upper end, the spring balancer (8) being hung with the upper end of the lifting plate guide rod (602).

7. The liquid suction device with pressure monitoring according to claim 5, characterized in that: The lower end of the pipe rack (6) is provided with a hollow part (605), the hollow part (605) is provided with a hinge shaft (606), one end of the push piece (601) is hinged with the hinge shaft (606), the push piece (601) is provided with a first counterbore (607), the side of the hinge shaft (606) away from the pipette connector (5) is provided with a blocking piece (609), the blocking piece (609) is provided with a second counterbore (610), and a spring (608) is further arranged, one end of the spring (608) is inserted into the first counterbore (607) and abuts against the bottom end of the first counterbore (607), and the other end of the spring (608) is inserted into the second counterbore (610) and abuts against the bottom end of the second counterbore (610).

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