Liquid loading device and biochemical reaction system
By designing the loading module and the liquid application module of the liquid loading device, the problems of difficult cleaning and cross-contamination in the existing technology are solved, realizing efficient, uniform application and low pollution of liquid loading, which is suitable for partitioned loading of open substrates.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-27
- Publication Date
- 2026-03-27
AI Technical Summary
Existing multi-functional liquid applicator tips have complex structures, are difficult to clean, and open slides make it difficult to load different liquids in different areas, resulting in cross-contamination and high reagent consumption.
A liquid loading device was designed, including a loading module and a liquid application module. The loading module directly drips liquid onto an open carrier through a loading tube, and the liquid application module coats the carrier with a liquid application plate to form a liquid film. The loading module and the liquid application module work together to achieve zoned loading and uniform application, reducing cleaning difficulty and cross-contamination.
It achieves efficient, uniform application and low contamination of liquid loading, is highly adaptable, and is suitable for partitioned loading of multiple sample capture areas, reducing cleaning difficulty and reagent consumption.
Smart Images
Figure CN224047344U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of biochemical reaction instrument and equipment, and particularly to a liquid loading device and a biochemical reaction system. BACKGROUND
[0002] The chip used by the second-generation sequencing technology is usually a flow cell, however, the reagent consumption is high during the liquid changing process of the flow cell, and less than 1% of the reagent participates in the reaction, and most of the reagent does not participate in the reaction. Therefore, an open chip is developed, which usually uses soaking, spin coating, spraying and other methods to load reagents, which can effectively reduce reagent consumption. At present, a multifunctional liquid smearing cutter head is mostly used to smear the liquid containing biological samples on the open chip.
[0003] However, the existing multifunctional liquid smearing cutter head has a complex structure and is difficult to clean, and the residual pollution on the cutter head will affect the biochemical reaction. In addition, due to the structural limitation of the open chip, it is difficult to load different liquids in different areas of the same chip. CONTENT OF THE INVENTION
[0004] In view of this, in order to solve at least one of the above defects, it is necessary to provide a liquid loading device.
[0005] In addition, the present application also provides a biochemical reaction system applying the aforementioned liquid loading device.
[0006] In the first aspect, the embodiments of the present application provide a liquid loading device for loading liquid on an open chip, the liquid loading device comprising: a loading module and a liquid smearing module. The loading module comprises at least one loading assembly, the loading assembly comprising a loading fixing seat and at least one loading pipe arranged on the loading fixing seat, the loading module being movable relative to the chip, the loading pipe being in communication with a liquid storage module and dropping liquid onto the chip. The liquid smearing module is movable relative to the chip, the liquid smearing module comprising at least one liquid smearing assembly, the liquid smearing assembly comprising a long strip-shaped liquid smearing sheet, the liquid smearing sheet facing the chip, the liquid smearing assembly being used for coating the liquid droplets dropped on the chip by the loading pipe to form a liquid film.
[0007] In some possible embodiments, the loading fixing seat comprises a first surface and a second surface arranged oppositely, the first surface being used for facing the chip, at least one channel being arranged in the loading fixing seat and penetrating through the first surface and the second surface, the loading pipe being embedded in the channel and extending out of the first surface.
[0008] In some possible embodiments, the channel has a curved structure, and in a direction extending along the channel, the channel comprises a first bending region and a second bending region connected in sequence, the first bending region protrudes towards the first surface, and the second bending region protrudes towards the second surface, so that the loading tube located in the channel forms a detour region.
[0009] In some possible embodiments, the loading seat comprises a first seat body and a second seat body connected detachably, and the first seat body and the second seat body jointly enclose the channel.
[0010] In some possible embodiments, the liquid wiping assembly comprises a first liquid wiping part and a second liquid wiping part connected detachably, the liquid sheet is located in the first liquid wiping part and / or the second liquid wiping part, and the first liquid wiping part and the second liquid wiping part jointly enclose a long strip-shaped gap, and an opening of the gap faces the carrier sheet.
[0011] In some possible embodiments, the gap is used to communicate with a power source, and under the driving of the power source, the liquid wiping assembly is further used to remove the liquid on the carrier sheet.
[0012] In some possible embodiments, the loading module comprises a plurality of loading assemblies arranged at intervals; and / or the liquid wiping module comprises a plurality of liquid wiping assemblies arranged at intervals.
[0013] In some possible embodiments, the liquid loading device further comprises a scheduling and transferring module connected with the loading module and the liquid wiping module respectively, and used to synchronously or independently move the loading module and the liquid wiping module.
[0014] In the second aspect, the embodiments of the present application provide a biochemical reaction system, comprising: a liquid storage module used to store liquid required by biochemical reaction; a carrier sheet having an open surface, the open surface comprising at least one sample capturing region; a power source; and the liquid loading device as described above. Wherein, the loading tube communicates with the liquid storage module and the power source, the loading tube is used to drop liquid to the sample capturing region, and the liquid wiping module is used to form a liquid film from a liquid drop on the sample capturing region.
[0015] In some possible embodiments, the carrier sheet comprises a plurality of sample capturing regions arranged at intervals; the loading module comprises a plurality of loading assemblies, each of the loading assemblies is used to drop a liquid drop for one of the sample capturing regions; and the liquid wiping module comprises a plurality of liquid wiping assemblies, each of the liquid wiping assemblies is used to form a liquid film from liquid on one of the sample capturing regions.
[0016] The liquid loading device provided in this application embodiment allows liquid to be directly dripped onto an open substrate via the loading tube on the loading module. Cleaning the loading tube is relatively easy, effectively reducing cross-contamination. The number of loading tubes fixed to the loading base can be increased according to the required level of contamination protection, allowing different liquids to be dripped through different loading tubes. Furthermore, the liquid-spreading module can evenly spread the dripped liquid onto the substrate, and can also smooth the liquid film during biochemical reactions, reducing the risk of film shrinkage. In addition, the cooperation between the loading module and the liquid-spreading module allows for adaptability to different open substrates, demonstrating strong adaptability and high loading efficiency. Attached Figure Description
[0017] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments of this application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0018] Figure 1 This is a modular architecture diagram of a biochemical reaction system according to an embodiment of this application.
[0019] Figure 2 This is a schematic diagram of the structure of a liquid loading device according to an embodiment of this application.
[0020] Figure 3 for Figure 2 A schematic diagram of the liquid loading device that performs liquid loading above the carrier.
[0021] Figure 4 for Figure 2 A schematic diagram of the loading module.
[0022] Figure 5 for Figure 4 An exploded view of part of the loaded module.
[0023] Figure 6 for Figure 5 A schematic diagram of the structure of the first seat in the middle.
[0024] Figure 7 for Figure 2 A schematic diagram of the structure of the liquid coating module.
[0025] Figure 8 for Figure 7 Sectional view along II-II.
[0026] Figure 9 for Figure 7 A schematic diagram of the structure of the first loading section.
[0027] Figure 10 For Figure 7 Structure diagram of second loading part.
[0028] Main component symbol explanation
[0029]
[0030] The following detailed description will further describe the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION
[0031] The technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments.
[0032] It should be noted that when a component is referred to as being "fixed" or "mounted" to another component, it can be directly on the other component or there can be an intermediate component between them. When a component is referred to as being "disposed" on another component, it can be directly disposed on the other component or there can be an intermediate component between them. The term "and / or" as used herein includes all and any combinations of one or more relevant items in the list of items.
[0033] Please refer to Figure 1 The biochemical reaction system 1000 provided by the embodiments of the present application can be used in the analysis process of the reaction and detection of biochemical substances in the fields of biology, chemistry or medicine, for example, can be used in the process of gene sequencing. The biochemical reaction system 1000 can include a liquid loading device 100, a liquid storage module 200, an open slide 300 and a power source 400. The liquid loading device 100 is used to load liquid onto the open slide 300 and / or remove liquid on the slide 300. Specifically, the liquid loading device 100 is in communication with the liquid storage module 200. The liquid storage module 200 is used to store liquid, which can be at least one of biological samples, reagents, buffers and the like required for biochemical reactions. The liquid loading device 100 is also in communication with the power source 400, which can provide driving force to enable the liquid in the liquid storage module 200 to enter the liquid loading device 100 and be loaded onto the slide 300 through the liquid loading device 100, and / or the power source 400 can also provide driving force to enable the liquid loading device 100 to remove liquid on the slide 300. The open slide 300 can be used for biochemical reactions, for example, the slide 300 can be a sequencing slide and can be used for gene sequencing reactions. Specifically, the liquid loading device 100 can be used for a floor-based or desktop-based sequencing system based on fluorescence imaging. It can be understood that the liquid loading device 100 can also be used for other biochemical liquid loading processes.
[0034] Please refer to Figure 3 As shown, the aforementioned open slide 300 has an open surface 301, which includes a sample capture area 302 for capturing and fixing a biological sample to be tested, and a partition 303 surrounding the sample capture area 302. The partition 303 can divide the open surface 301 into one or more sample capture areas 302. Specifically, the sample capture area 302 has a large number of capture sites arranged in an array for capturing and fixing a biological sample to be tested, and mainly serves as a reaction area of the slide 300 to provide a place for biochemical reactions of the biological sample to be tested. The biological sample to be tested and the required reagents perform biochemical reactions in the sample capture area 302, and then the light signal emitted by the product sample can be detected to achieve detection of the product. The partitioning method of the slide 300 can use hydrophobic coating to form the partition 303 to separate different sample capture areas 302 of the slide 300, or use other processing techniques to make different sample capture areas 302 protrude or recess.
[0035] Please refer to Figure 2 With Figure 3As shown, the liquid loading device 100 comprises a loading module 10 and a liquid wiping module 20. The loading module 10 comprises at least one loading assembly 5, the loading assembly 5 comprises a loading fixing seat 1 and at least one loading tube 2 arranged on the loading fixing seat 1, the loading module 10 is movable relative to the slide 300, the loading tube 2 is in communication with the liquid storage module 200 and is used for dropping liquid on the slide 300. Specifically, the loading module 10 is generally located above the open surface 301 of the slide 300 and can move relative to the slide 300, and during the relative movement, the loading tube 2 can drop the required liquid on the sample capture area 302 of the slide 300, and the liquid drop on the slide 300 is generally in the form of a liquid drop. The liquid wiping module 20 is movable relative to the slide 300, and the liquid wiping module 20 comprises at least one liquid wiping assembly 3. The liquid wiping assembly 3 comprises a long strip-shaped liquid wiping piece 33, the liquid wiping piece 33 faces the slide 300, and the liquid wiping assembly 3 is used for coating the liquid drop on the slide 300 to form a liquid film (very thin liquid film). Specifically, by arranging the loading fixing seat 1, the loading tube 2 can be fixed, and the loading tube 2 is in communication with the liquid storage module 200, and the liquid is dropped on the open slide 300 through the loading tube 2, and the liquid is only in the loading tube 2 and does not enter the loading fixing seat 1, so that only the loading tube 2 needs to be cleaned during cleaning, the cleaning difficulty is reduced, and pollution is reduced.
[0036] In addition, the liquid wiping assembly 3 further comprises a long strip-shaped gap 4, the opening 41 of the gap 4 faces the slide 300, the gap 4 can be in communication with the power source 400, and the liquid wiping assembly 3 further removes the liquid on the slide 300 through the gap 4. Specifically, the gap 4 can be provided with a certain negative pressure by the power source 400 to suck away the liquid (such as waste liquid after reaction) on the slide 300.
[0037] In some embodiments, the loading module 10 and the liquid spreading module 20 can be driven to move relative to the slide 300. In this case, the liquid loading device 100 can further comprise a scheduling transfer module 30, which can be connected with the loading module 10 and the liquid spreading module 20 to drive the loading module 10 and the liquid spreading module 20 to move synchronously or independently. For example, the scheduling transfer module 30 can drive the loading module 10 to move above the slide 300 and move above the slide 300 to drop liquid droplets on the surface of the slide 300. The scheduling transfer module 30 can also drive the liquid spreading module 20 to move above the slide 300 and move above the slide 300 to spread the liquid droplets on the slide 300 into a liquid film, and to remove the liquid on the slide 300.
[0038] Please refer to Figures 4 to 6 As shown, the loading fixture 1 comprises a first surface 11 and a second surface 12 arranged oppositely, and the first surface 11 is used to face the slide 300. That is, during liquid loading, the first surface 11 of the loading fixture 1 faces the surface of the slide 300 to facilitate loading of liquid on the slide 300. The loading fixture 1 is provided with at least one channel 13 penetrating through the first surface 11 and the second surface 12, and the loading tube 2 is embedded in the channel 13 and extends out of the first surface 11. It can be understood that the loading tube 2 can be a hose to be embedded in the channel 13. Embedding the loading tube 2 in the channel 13 of the loading fixture 1 can improve the stability of the loading tube 2 during liquid loading above the slide 300. In addition, the loading tube 2 extends out of the first surface 11 of the loading fixture 1, which can ensure that the liquid only contacts the loading tube 2 during droplet loading, and the liquid flowing out of the loading tube 2 does not contact the loading fixture 1, which can further reduce the difficulty of cleaning and reduce pollution.
[0039] In addition, according to the area of the slide 300 requiring liquid loading and the amount of liquid loading, the number of loading tubes 2 on the loading fixture 1 can be flexibly set. For example, one channel 13 can be provided on the loading fixture 1 to embed one loading tube 2, or multiple channels 13 can be provided to embed multiple loading tubes 2. For example, according to the level of pollution protection requirement, the number of loading tubes 2 on each loading assembly 5 can be increased, one loading tube 2 is used to drop one kind of liquid, and another loading tube 2 is used to drop another kind of liquid, which can further reduce the frequency of cleaning and reduce pollution.
[0040] In some embodiments, the loading tube 2 can be detachably arranged in the channel 13, so that the loading tube 2 can be maintained and replaced as needed to further reduce pollution and improve convenience.
[0041] As Figure 5 and Figure 6As shown, the channel 13 is in a curved structure, so the loading tube 2 embedded in the channel 13 is also in a curved structure. Further, along the extension direction of the channel 13, the channel 13 comprises a first bending area 14 and a second bending area 15 connected in sequence, the first bending area 14 is convex to the first surface 11, and the second bending area 15 is convex to the second surface 12, so that after embedding the loading tube 2 in such a channel 13, a detour area A is formed on the channel 13, which is beneficial to buffer the liquid and improve the controllability of dropping the droplets, so as to prevent the droplets from being dropped too quickly and the amount of the liquid being dropped from being out of control.
[0042] As shown in the figure, Figure 5 As shown in the figure, Figure 6 As shown, the loading fixture 1 comprises a first seat body 16 and a second seat body 17 which are detachably connected, and the first seat body 16 and the second seat body 17 jointly enclose the channel 13. Specifically, along a first direction X from the second surface 12 to the first surface 11, the first seat body 16 comprises a first part 161 and a second part 162 connected to each other, the second surface 12 is located on the first part 161, and the first surface 11 is located on the first part 161 and the second part 162. Along a second direction Y perpendicular to the first direction X, the thickness of the first part 161 is greater than the thickness of the second part 162, so that the first part 161 and the second part 162 form a stepped groove 163, and the second seat body 17 is located at the stepped groove 163 and detachably connected with the second part 162. When a channel 13 is arranged in the loading fixture 1, a through hole 164 is arranged through the first part 161 along the first direction X, and a groove 165 is arranged on the second part 162, and the through hole 164 and the groove 165 are connected and constitute the channel 13. When installing the loading tube 2, the loading tube 2 can first pass through the through hole 164, the through hole 164 can first fix the loading tube 2, and then the loading tube 2 is embedded in the groove 165, so that the stability of the loading tube 2 is better when assembling the second seat body 17, and the assembly is easy; moreover, the formation of the channel 13 is facilitated.
[0043] As shown in the figure, Figure 3 As shown, the loading module 10 can comprise a plurality of loading assemblies 5 arranged at intervals, and each loading assembly 5 is used to load droplets to a sample capture area 302, so that a plurality of loading assemblies 5 can simultaneously load droplets to a plurality of different sample capture areas 302. It can be understood that the plurality of loading assemblies 5 can load the same liquid, or can load different liquids to realize partitioned loading on the slide 300.
[0044] As shown in the figure, Figures 1 to 3As shown, the loading module 10 further comprises a loading mounting plate 6, the loading fixture 1 is mounted on the loading mounting plate 6, and the loading mounting plate 6 can be further connected to the dispatching and transferring module 30. The loading mounting plate 6 can drive the loading fixture 1 to move along the first moving direction a (e.g. the length direction of the slide 300) on the surface of the slide 300. When multiple linear and side-by-side droplets need to be output on the surface of the slide 300, the loading mounting plate 6 can be stretched or contracted to drive the loading fixture 1 to move along the second moving direction b (e.g. the width direction of the slide 300) so that multiple droplets are formed on the surface of the slide 300, and further, the multiple droplets can be connected and fused together to form a long droplet. The second moving direction b can be perpendicular to the first moving direction a. Figure 2 Figure 2
[0045] Please refer to Figure 1 , Figure 2 and Figure 5 again. The hollow loading tube 2 can be an integrated structure, one end of which is connected to the liquid storage module 200, and the other end is fixed to the loading fixture 1 and extends from the loading fixture 1. In this way, the liquid only contacts the loading tube 2, and only the loading tube 2 needs to be cleaned during cleaning, which is relatively low in difficulty. In some embodiments, as mentioned above, the loading tube 2 can be a hose, which is convenient for installation and movement.
[0046] Please refer to Figures 7 to 10 together. The liquid wiping assembly 3 comprises a first liquid wiping part 31 and a second liquid wiping part 32 which are detachably connected, and the liquid wiping sheet 33 can be formed on the first liquid wiping part 31 and / or the second liquid wiping part 32. The first liquid wiping part 31 and the second liquid wiping part 32 can be oppositely arranged along the first moving direction a. Specifically, the liquid wiping sheet 33 can be formed on the end of the first liquid wiping part 31 or the second liquid wiping part 32 which faces the slide 300, so that when the liquid wiping assembly 3 moves, the long strip-shaped liquid wiping sheet 33 can form the longest liquid bridge with the slide 300 to improve the liquid wiping speed and the uniformity of the liquid film. The liquid wiping sheet 33 can also be arranged on the end of the first liquid wiping part 31 and the second liquid wiping part 32 which faces the slide 300, so that when the liquid wiping assembly 3 reciprocally moves along the first moving direction a, liquid wiping can be achieved.
[0047] In some embodiments, the first liquid application part 31 and the second liquid application part 32 jointly enclose the gap 4. Specifically, the surface of the first liquid application part 31 towards the second liquid application part 32 forms a shallow groove structure, which, after being assembled with the second liquid application part 32, encloses the gap 4. In the liquid application mode, the width of the gap 4 is small, which can further form a capillary effect, strengthen the ability to absorb liquid droplets, and further facilitate the formation of a liquid bridge between the sample capture area 302 of the slide 300, and then uniformly apply the liquid droplets on the sample capture area 302. It should be noted that the capillary phenomenon of the gap 4 only further facilitates the formation of a liquid bridge, and in fact the liquid bridge phenomenon does not necessarily have to be a capillary phenomenon. In addition, in the liquid removal mode, the gap 4 can be provided with negative pressure by the power source 400, so as to suck away the waste liquid on the slide 300. It can be understood that the gap 4 can also be blown by the power source 400, so as to blow away the waste liquid on the slide 300 to achieve the purpose of liquid removal.
[0048] In some embodiments, the liquid application assembly 3 further comprises a long strip-shaped cavity 42, which is in communication with the gap 4, and the cavity 42 can be in communication with the power source 400. For example, the cavity 42 and the gap 4 can be in a negative pressure state by the power source 400, so as to suck away the liquid on the slide 300. The liquid sucked by the gap 4 can be stored in the cavity 42, and then further discharged from the liquid application assembly 3. In addition, for the air blowing liquid removal mode, the cavity 42 can be used to store gas, so that the gap 4 can uniformly blow out air.
[0049] The liquid application module 20 can comprise a plurality of spaced liquid application assemblies 3, and the plurality of liquid application assemblies 3 are in an integrated structure. That is, a plurality of liquid application pieces 33 are spaced, and each liquid application piece 33 corresponds to one sample capture area 302 on the slide 300, so as to achieve uniform application of liquid droplets on a plurality of sample capture areas 302, and improve the liquid application efficiency.
[0050] In some embodiments, when the liquid application module 20 comprises a plurality of liquid application assemblies 3, the liquid application piece 33 can be formed on the first liquid application part 31 and the second liquid application part 32, and a plurality of spaced groove structures can be formed on the first liquid application part 31. Then, the second liquid application part 32 is covered on the first liquid application part 31 to jointly enclose a plurality of independently arranged gaps 4, and each gap 4 corresponds to one sample capture area 302 on the slide 300, so as to achieve uniform application of liquid droplets on a plurality of sample capture areas 302 and liquid removal.
[0051] The liquid application module 20 further comprises a liquid application mounting plate 7, which can be mounted on the dispatching and transferring module 30.
[0052] In use, the liquid loading process of a single sample capture area 302 comprises the following steps:
[0053] In the first step, the loading module 10 and the liquid wiping module 20 can be moved above the sample capture area 302 of the slide 300 by the dispatching and transferring module 30. The loading module 10 and the liquid wiping module 20 can be arranged side by side along the first moving direction a of the loading module 10, and the loading module 10 can be located in front of the moving path. During the loading process, the loading module 10 is moved above the sample capture area 302 of the slide 300 by the dispatching and transferring module 30, and a droplet is added to the sample capture area 302 by the loading tube 2. It can be understood that the droplet can be only one droplet. If the surface of the slide 300 is large and the area of the sample capture area 302 is large, a plurality of droplets arranged in a row can be loaded on the sample capture area 302. At this time, the loading assembly 5 is moved on the surface of the slide 300 along the second moving direction b by the telescopic loading mounting plate 6, so that a plurality of droplets are formed on the surface of the slide 300. Further, the plurality of droplets can be connected and fused together to form a long droplet.
[0054] In the second step, after the addition is completed, the droplet on the sample capture area 302 is evenly wiped by the liquid wiping module 20 driven by the dispatching and transferring module 30, so as to form a uniform liquid film. Since the liquid loading is realized by the loading module 10, the liquid wiping module 20 is mainly used to evenly wipe the droplet, and the cleaning difficulty is small and easy to clean, which can further reduce the pollution.
[0055] In the third step, after the reaction is completed, the liquid wiping module 20 can be moved above the sample capture area 302 by the dispatching and transferring module 30. As described above, the waste liquid on the slide 300 can be removed by the liquid suction or air blowing.
[0056] It can be understood that, during the loading process, the loading module 10 can be moved above the sample capture area 302 of the slide 300 by the dispatching and transferring module 30, and a droplet can be added to the sample capture area 302 by the loading tube 2. After the addition is completed, the loading module 10 is removed by the dispatching and transferring module 30, and the liquid wiping module 20 is moved above the sample capture area 302, and the droplet on the sample capture area 302 is evenly wiped to form a uniform liquid film.
[0057] Of course, in the above embodiment, the loading module 10 and the liquid wiping module 20 are moved, and the slide 300 is stationary. In other embodiments, the loading module 10 and the liquid wiping module 20 can be stationary, and the slide 300 can be moved. The loading module 10, the liquid wiping module 20 and the slide 300 can all be moved. In summary, the loading module 10 and the liquid wiping module 20 can move relative to the slide 300.
[0058] The conventional slide usually has only one sample capture area, and only one reagent needs to be loaded during the liquid loading process. For example, the sample capture area 302 of the slide 300 is loaded with a droplet of a reagent by the loading module 10, and then the droplet is evenly wiped by the liquid wiping module 20 to form a uniform liquid film. After the reaction is completed, the waste liquid on the slide 300 is removed by the liquid wiping module 20. Figure 2As shown, the slide 300 of the present application can have a plurality of sample capture areas 302, and it is difficult to implement partitioned liquid loading in a conventional liquid loading manner, and it is more difficult to implement partitioned loading of different liquids. Therefore, the aforementioned liquid loading device 100 provided by the present application can implement partitioned liquid loading of the slide 300, and can also implement partitioned loading of different liquids. Specifically, the loading module 10 can include a plurality of loading assemblies 5 arranged at intervals, and each loading assembly 5 is used to load a sample capture area 302 with a droplet, so that a plurality of loading assemblies 5 can simultaneously load a plurality of different sample capture areas 302 with droplets. Of course, one loading assembly 5 can also load a plurality of sample capture areas 302 with droplets by extending and retracting the loading mounting plate 6 along the second moving direction b. It can be understood that the plurality of loading assemblies 5 can load the same liquid, or can load different liquids to achieve partitioned loading on the slide 300. At the same time, the liquid wiping module 20 can include a plurality of liquid wiping assemblies 3 arranged at intervals, each liquid wiping assembly 3 can cooperate with a loading assembly 5 to evenly spread the droplet to form a uniform liquid film on the sample capture area 302, and can also remove the waste liquid on the sample capture area 302. This partitioned mode can reduce cross contamination between different sample capture areas 302.
[0059] Therefore, the timing of partitioned liquid loading is as follows:
[0060] Firstly, the loading module 10 is moved to the upper side of the slide 300 by the scheduling transfer module 30, and a plurality of loading assemblies 5 correspond to a plurality of sample capture areas 302 respectively, at this time, the liquid wiping module 20 is controlled to be located outside the slide 300 by the scheduling transfer module 30.
[0061] Secondly, different liquids are injected into different loading tubes 2 fixed by different loading assemblies 5 respectively, and the loading module 10 is moved above the slide 300 by the scheduling transfer module 30 (the slide 300 can also be moved while the loading module 10 is stationary), and different reagents are dropped on different sample capture areas 302 at one time.
[0062] It can be understood that if there is only one loading assembly 5 in the loading module 10, the loading module 10 can also be moved along the second moving direction b by the scheduling transfer module 30 to drop the liquid on different sample capture areas 302.
[0063] Thirdly, after the liquid dropping is completed, the loading module 10 is moved out of the upper side of the slide 300 by the scheduling transfer module 30, and the liquid wiping module 20 is placed above the slide 300. A plurality of liquid wiping assemblies 3 in the liquid wiping module 20 correspond to different sample capture areas 302 respectively, and form liquid bridges with the sample capture areas 302, and the slide 300 and the liquid wiping assemblies 3 move relatively in the first moving direction a, so that the liquid is uniformly distributed in the plurality of sample capture areas 302.
[0064] In addition, when the reaction time is longer, the liquid bridge can be formed between the wiping assembly 3 and the surface of the sample capture area 302, and the sample capture area 302 is moved back and forth along the first moving direction a, so as to prevent the liquid film on the sample capture area 302 from evaporating and shrinking.
[0065] In the fourth step, after the reaction is completed, the wiping module 20 can be controlled by the scheduling transfer module 30 to remove the waste liquid on the slide 300.
[0066] In the liquid loading device 100 provided by the embodiment of the present application, the loading tube 2 on the loading module 10 can directly drop the liquid on the open slide 300, the cleaning difficulty of the loading tube 2 is small, and the cross contamination problem can be effectively reduced; the number of loading tubes 2 on each loading assembly 5 can be increased according to the level of contamination protection requirement, and different liquids can be dropped through different loading tubes 2. The wiping assembly 3 in the wiping module 20 can uniformly spread the liquid drops dropped on the slide 300, and can also remove the liquid on the slide 300, and the loading liquid does not need to use the wiping module 20, the cleaning difficulty of the wiping module 20 is small, and the pollution can be further reduced. In addition, the liquid loading device 100 of the present application has strong expansibility, and through the cooperation of the loading module 10 and the wiping module 20, different open slides 300 can be adapted, especially the partition loading with multiple sample capture areas 302 can be adapted, the adaptability is strong, and the loading efficiency is high.
[0067] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and not to limit, although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.
Claims
1. A liquid loading device for loading liquid onto an open slide, characterized in that, The liquid loading device comprises: a loading module comprising at least one loading assembly, the loading assembly comprising a loading fixing base and at least one loading tube arranged on the loading fixing base, the loading module being movable relative to the slide, the loading tube being in communication with a liquid storage module and being used to drop liquid onto the slide; and a liquid wiping module, the liquid wiping module being movable relative to the slide, the liquid wiping module comprising at least one liquid wiping assembly, the liquid wiping assembly comprising a long strip-shaped liquid wiping piece, the liquid wiping piece facing the slide, the liquid wiping assembly being used to spread the liquid drop dropped on the slide by the loading tube to form a liquid film.
2. The liquid loading device of claim 1, wherein, The loading fixing base comprises oppositely arranged first and second surfaces, the first surface being used to face the slide, the loading fixing base being provided with at least one channel penetrating through the first and second surfaces, the loading tube being embedded in the channel and extending out of the first surface.
3. The liquid loading device of claim 2, wherein, The channel has a curved structure, and in the direction along which the channel extends, the channel comprises sequentially connected first and second bending regions, the first bending region being convex towards the first surface, and the second bending region being convex towards the second surface, so that the loading tube located in the channel forms a detour region.
4. The liquid loading device of claim 2, wherein, The loading fixing base comprises detachably connected first and second seat bodies, and the first and second seat bodies jointly enclose the channel.
5. The liquid loading device of claim 1, wherein, The liquid wiping assembly comprises detachably connected first and second liquid wiping parts, the liquid wiping piece being located in the first and / or second liquid wiping part, the first and second liquid wiping parts jointly enclosing a long strip-shaped gap, and the opening of the gap facing the slide.
6. The liquid loading device of claim 5, wherein, The gap is used to communicate with a power source, and under the driving of the power source, the liquid wiping assembly is further used to remove the liquid on the slide.
7. The liquid loading device of claim 1, wherein, The loading module comprises a plurality of spaced loading assemblies; and / or the liquid wiping module comprises a plurality of spaced liquid wiping assemblies.
8. The liquid loading device of claim 1, wherein, Further comprising a scheduling transfer module connected with the loading module and the liquid wiping module respectively, and used to synchronously or independently move the loading module and the liquid wiping module.
9. A biochemical reaction system, characterized by, comprise: a liquid storage module used to store liquid required by biochemical reaction; a slide having an open surface, the open surface comprising at least one sample capturing region; a power source; and The liquid loading device according to any one of claims 1 to 8, wherein the loading tube is in communication with the liquid storage module and the power source, the loading tube being used to drop liquid to the sample capturing region, and the liquid wiping module being used to form a liquid film from the liquid drop on the sample capturing region. The slide comprises a plurality of spaced sample capturing regions; 10. The biochemical reaction system of claim 9, wherein, The loading module comprises a plurality of loading assemblies, each of the loading modules being used to drop liquid drop for one of the sample capturing regions; The liquid wiping module comprises a plurality of liquid wiping assemblies, each of the liquid wiping modules being used to form a liquid film from the liquid on one of the sample capturing regions.