High-throughput culture instrument for intestinal flora
The high-throughput gut microbiota culture instrument, with its partitioned heating and modular design, solves the problems of low efficiency and cross-contamination in traditional equipment, achieving efficient and accurate gut microbiota culture.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-22
- Publication Date
- 2026-03-27
AI Technical Summary
Traditional gut microbiota culture equipment suffers from problems such as low culture efficiency, inaccurate temperature control, and easy cross-contamination between different microbiota.
It adopts a zoned heating and modular petri dish design, combined with a precise positioning structure to ensure that each petri dish corresponds to the heating plate, achieving independent temperature control, and prevents culture medium contamination and shedding through sealing heads and limiting nets.
It improves the efficiency and accuracy of gut microbiota culture, avoids temperature interference and cross-contamination between microbiota, and ensures the stability and reliability of culture results.
Smart Images
Figure CN224047372U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the technical field of intestinal microorganism research, and specifically relates to an intestinal flora high-throughput culture instrument. BACKGROUND
[0002] In the field of intestinal microorganism research, the cultivation of intestinal flora is a crucial link. Intestinal flora is not only closely related to human health, but also has wide application value in disease diagnosis, drug development and probiotic research. However, traditional intestinal flora cultivation methods have many limitations, such as low cultivation efficiency, inaccurate temperature control, and cross contamination between different flora, which seriously limit the progress of research and the effect of application.
[0003] Traditional culture equipment usually uses a single heating source, which cannot heat different types of intestinal flora in different zones, resulting in different flora being cultured at the same temperature, which cannot meet their respective optimal growth conditions. In addition, the design of traditional culture dishes is relatively simple, lacking precise positioning and limiting structures, which can easily lead to uneven distribution or shedding of the culture medium, affecting the stability and reliability of the culture results.
[0004] Based on this, the present patent proposes an innovative intestinal flora high-throughput culture instrument, which significantly improves the efficiency, accuracy and operational convenience of intestinal flora cultivation through zoned heating, modular culture dish design and precise positioning structure. SUMMARY
[0005] The utility model aims at providing an intestinal flora high-throughput culture instrument to solve the problems of low cultivation efficiency, inaccurate temperature control and cross contamination between different flora of traditional intestinal flora culture instruments.
[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: an intestinal flora high-throughput culture instrument, comprising a heating instrument, a heating groove is arranged at the center of the upper end of the heating instrument, a limiting shell is connected at the inner upper end of the heating groove, a control console is arranged at the front side of the upper end of the heating instrument, and the control console is composed of a display screen, control buttons and a control knob, four heating plates are arranged inside the lower end of the heating groove, and the heating plates are controlled by the control console, a packaging shell is threadedly connected to the inner upper end of the limiting shell and forms a culture dish with the packaging shell.
[0007] Preferably, an assembly handle groove is arranged at the center of the upper end of the packaging shell, a positioning clamping strip is arranged between the four heating plates, and the positioning clamping strip is fixedly connected to the inside of the heating groove.
[0008] Preferably, the lower end of the limiting shell is internally provided with a positioning clamping groove, and the positioning clamping groove is clamped on the upper end of the positioning clamping strip.
[0009] Preferably, the four culture grooves correspond to the four heating plates one by one, and are respectively located above the four heating plates, and the lower end of the positioning clamping groove is provided with a liquid adding hole, and the liquid adding hole is in communication with the inside of the culture dish.
[0010] Preferably, the liquid adding hole is internally threadedly connected with a plugging head, and the plugging head is completely located inside the liquid adding hole, and the lower end of the plugging head is provided with a plugging buckle slot.
[0011] Preferably, the lower end of the packaging shell is provided with a limiting frame, and the limiting frame is located below the four culture grooves, and the outer portion of the limiting frame is provided with a plurality of limiting clamping blocks, and the upper end of the limiting shell is internally provided with a plurality of limiting clamping grooves.
[0012] Preferably, the plurality of limiting clamping blocks are respectively clamped inside the plurality of limiting clamping grooves, and the inner wall of the limiting frame is fixedly connected with four connecting frames, and the upper end of the four connecting frames is fixedly connected with a limiting net.
[0013] Preferably, the four limiting nets are respectively located inside the four culture grooves and are respectively located above the four heating plates.
[0014] Compared with the prior art, the utility model provides a kind of intestinal flora high flux culture instrument, with the following beneficial effects:
[0015] 1, the utility model discloses a plurality of independent heating plates are set, and culture dish is accurately fixed in heating groove by positioning clamping strip and positioning clamping groove, ensure that each culture groove corresponds with heating plate completely, different types of intestinal flora can be heated by partition, ensure that each flora grows at optimum temperature, not only improve culture efficiency, also avoid temperature interference between different flora.
[0016] 2, the utility model discloses culture dish and adopts modular design, and quick assembly and disassembly are realized by screw connection, convenient operation and cleaning, culture dish is internally provided with a plurality of independent culture grooves, each culture groove corresponds to a heating area, ensure that flora does not interfere with each other in the process of culture.
[0017] 3, the utility model discloses culture dish bottom is provided with liquid adding hole and plugging head, and culture medium can be conveniently added by liquid adding hole, and sealing is realized by plugging head, prevent culture medium from spilling or contaminating heating area, ensure the purity of culture medium and the health of culture environment.
[0018] 4. The petri dish of this utility model is equipped with a limiting net to ensure that the culture medium can be evenly distributed and completely cover the limiting net after being added, preventing the culture medium from falling out of the culture tank after solidification, ensuring the stability of the culture medium position during the culture process, thereby improving the reliability of the culture.
[0019] 5. The packaging shell of this utility model culture instrument is provided with an assembly handle groove, which can be used to quickly assemble and disassemble the culture dish by rotating it. After the culture is completed, the culture dish can be quickly disassembled by removing the packaging shell and taking out the limiting frame, which facilitates thorough cleaning and disinfection of each component. Attached Figure Description
[0020] Figure 1 This is a three-dimensional structural diagram of the high-throughput culture instrument of this utility model.
[0021] Figure 2 This is a schematic diagram of the heating tank connection structure of this utility model.
[0022] Figure 3 This is a schematic diagram of the culture dish in the flipped state of this utility model.
[0023] Figure 4 This is a schematic diagram of the disassembled structure of the culture dish of this utility model.
[0024] In the diagram: 1. Heating device; 2. Heating tank; 3. Limiting shell; 4. Control console; 5. Heating plate; 6. Encapsulation shell; 7. Assembly handle groove; 8. Positioning strip; 9. Positioning slot; 10. Culture tank; 11. Liquid filling hole; 12. Sealing head; 13. Sealing handle groove; 14. Limiting frame; 15. Limiting block; 16. Limiting slot; 17. Connecting frame; 18. Limiting mesh. Detailed Implementation
[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0026] This utility model provides, for example Figures 1-4The shown one kind of enteric flora high flux incubator, including heating instrument 1, heating instrument 1 upper end center is provided with heating groove 2, the inside joint of heating groove 2 upper end is limited to the shell 3, heating instrument 1 upper end front side is provided with control console 4, and control console 4 is by display screen, control button and control knob are formed, heating groove 2 lower end inside is provided with four heating plate 5, and heating plate 5 is controlled through control console 4, the inside screw thread connection of limited shell 3 upper end is sealed shell 6, and with sealed shell 6 incubator is formed, in the process of carrying out high flux culture to enteric flora, incubator is by heating instrument 1 and incubator is formed, the incubator of sealed shell 6 and sealed shell 6 is turned over, then the culture medium is melted and is poured into the inside incubator and is cooled and solidified, and the strain of different kinds of enteric flora is seeded on the culture medium, and the incubator is turned over again and is inserted into the inside heating groove 2, finally through control console 4 control four heating plate 5 to the strain of different kinds of enteric flora is heated in partition, so that different kinds of enteric flora can be cultured at the corresponding temperature simultaneously, so that the high flux culture of enteric flora is carried out.
[0027] Preferably, the upper end center of the sealed shell 6 is provided with an assembly hand slot 7, and the sealed shell 6 can be rotated through the assembly hand slot 7 during the assembly of the incubator, so as to be combined with the limited shell 3.
[0028] Preferably, the four heating plates 5 are provided with a positioning clamping strip 8, and the positioning clamping strip 8 is fixedly connected inside the heating groove 2, the lower end of the limiting shell 3 is provided with a positioning clamping groove 9, and the positioning clamping groove 9 is clamped on the upper end of the positioning clamping strip 8, the lower end of the limiting shell 3 is provided with four culture grooves 10, and the positioning clamping groove 9 is located between the four culture grooves 10, the four culture grooves 10 correspond to the four heating plates 5 one by one, and are located above the four heating plates 5 respectively, and the strains of different kinds of enteric flora can be seeded in the culture medium in the four culture grooves 10 respectively, so as to be seeded in partition for different kinds of enteric flora, avoiding mutual influence in the culture process.
[0029] At the same time, the incubator can be positioned with the four heating plates 5 through the positioning clamping strip 8 and the positioning clamping groove 9, so that the four culture grooves 10 can be located just above the four heating plates 5, so as to ensure that the four heating plates 5 can heat the enteric flora in the four culture grooves 10 respectively, so as to heat the enteric flora of different kinds at different temperatures, so as to ensure that the enteric flora of different kinds can be cultured at appropriate temperature, improve the culture efficiency of enteric flora, and the heating temperature of the four heating plates 5 can be controlled separately through the control button and the control knob of the control console 4, and the corresponding temperature is displayed through the display screen.
[0030] Preferably, a liquid adding hole 11 is arranged at the center of the lower end of the positioning slot 9, and the liquid adding hole 11 is in communication with the inside of the culture dish. A plug 12 is threadedly connected to the inside of the liquid adding hole 11, and the plug 12 is completely located in the liquid adding hole 11. A plug handle slot 13 is arranged at the lower end of the plug 12. When the melted culture medium is added to the culture dish, the plug 12 is screwed out through the plug handle slot 13, and the melted culture medium is added through the plug 12. This prevents the lower end opening of the culture tank 10 from being contaminated during the addition of the culture medium, and the amount of the added culture medium does not exceed the position of the lower end opening of the culture tank 10. Then the plug 12 is screwed in again, so that the culture medium does not come into contact with the plurality of heating plates 5 and the positioning clamping strip 8, preventing the culture medium from being contaminated.
[0031] Preferably, a limiting frame 14 is arranged at the lower end of the packaging shell 6, and the limiting frame 14 is located below the four culture tanks 10. A plurality of limiting clamping blocks 15 are arranged outside the limiting frame 14. A plurality of limiting clamping slots 16 are arranged inside the upper end of the limiting shell 3. The plurality of limiting clamping blocks 15 are respectively clamped inside the plurality of limiting clamping slots 16. During the assembly of the culture dish, the limiting frame 14, the four connecting frames 17 and the four limiting nets 18 can form a limiting frame. The limiting frame is prevented from being located inside the upper end of the limiting shell 3, and is limited by the plurality of limiting clamping blocks 15 and the plurality of limiting clamping slots 16. The four limiting nets 18 are located just inside the four culture tanks 10. The limiting frame is completely fixed by assembling the packaging shell 6.
[0032] Preferably, the limiting frame 14 is fixedly connected to four connecting frames 17 on the inner wall. The four connecting frames 17 are fixedly connected to limiting nets 18 on the upper end. The four limiting nets 18 are respectively located inside the four culture tanks 10 and above the four heating plates 5. During the process of adding the melted culture medium to the culture dish, the culture medium does not exceed the position of the lower end opening of the culture tank 10, and the culture medium completely submerges the four limiting nets 18. After the culture medium solidifies, the limiting frame and the culture dish are limited, and the solidified culture medium is limited inside the four culture tanks 10. This prevents the solidified culture medium from separating from the lower end opening of the culture tank 10 due to the large opening of the lower end of the culture tank 10, ensures the stability of the position of the culture medium, and ensures the stability of the culture process of the intestinal flora.
[0033] Preferably, after the culture is completed, the culture dish is disassembled by unscrewing the packaging shell 6 and removing the limiting frame, so that the culture dish can be more conveniently cleaned and disinfected.
[0034] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.
Claims
1. A high-throughput incubator for gut microbiota, characterized in that, Including heating instrument (1), heating groove (2) is arranged at the upper end center of heating instrument (1), limit shell (3) is clamped inside the upper end of heating groove (2), control console (4) is arranged at the upper end front side of heating instrument (1), and control console (4) is composed of display screen, control button and control knob, four heating plates (5) are arranged inside the lower end of heating groove (2), and heating plate (5) is controlled by control console (4), and packaging shell (6) is threadedly connected inside the upper end of limit shell (3) and is composed of culture dish with packaging shell (6).
2. The high-throughput culture apparatus for intestinal flora according to claim 1, characterized in that: Assembling buckle groove (7) is arranged at the upper end center of the packaging shell (6), positioning clamping strip (8) is arranged between the four heating plates (5), and the positioning clamping strip (8) is fixedly connected inside the heating groove (2).
3. The high-throughput culturing apparatus for intestinal flora according to claim 2, characterized in that: Positioning clamping groove (9) is opened in the lower end inside the limit shell (3), and the positioning clamping groove (9) is clamped on the upper end outside of the positioning clamping strip (8), four culture grooves (10) are opened in the lower end inside the limit shell (3), and the positioning clamping groove (9) is located between the four culture grooves (10).
4. The high-throughput culturing apparatus for intestinal flora according to claim 3, characterized in that: The four culture grooves (10) correspond to the four heating plates (5) one by one and are located above the four heating plates (5) respectively, liquid adding hole (11) is opened at the lower end center of the positioning clamping groove (9), and the liquid adding hole (11) is communicated with the inside of the culture dish.
5. The high-throughput culturing apparatus for intestinal flora according to claim 4, characterized in that: The plugging head (12) is threadedly connected inside the liquid adding hole (11), and the plugging head (12) is completely located inside the liquid adding hole (11), the plugging buckle groove (13) is arranged at the lower end center of the plugging head (12).
6. The high-throughput culturing apparatus for intestinal flora according to claim 5, characterized in that: The limit frame (14) is arranged at the lower end of the packaging shell (6), and the limit frame (14) is located below the four culture grooves (10), a plurality of limit clamping blocks (15) are arranged outside the limit frame (14), a plurality of limit clamping grooves (16) are opened in the upper end inside the limit shell (3).
7. The high-throughput culturing apparatus for enteric flora according to claim 6, characterized in that: A plurality of limit clamping blocks (15) are clamped inside a plurality of limit clamping grooves (16) respectively, four connecting frames (17) are fixedly connected to the inner wall of the limit frame (14), and the upper end of the four connecting frames (17) is fixedly connected with limit net (18).
8. The high-throughput culturing apparatus for intestinal flora according to claim 7, characterized in that: The four limit nets (18) are located inside the four culture grooves (10) respectively and are located above the four heating plates (5) respectively.