Cell culture bottle

Abstract

The utility model provides a cell culture bottle and belongs to the technical field of cell culture. Comprising a bottle body and a bottle cap arranged on the bottle body, a liquid taking pipe is coaxially arranged on the bottle cap in a sliding mode, one end of the liquid taking pipe extends into the bottle body, a piston is connected to the outer wall of a pipe section, the other end of the liquid taking pipe extends out of the bottle cap, then is bent downwards and is connected with a plugging head, and a liquid guide pipe used for being matched with the liquid taking pipe in an inserted mode is arranged at the bottom of the bottle body. A plurality of liquid guide holes are formed in the side wall of the liquid guide pipe. According to the cell culture bottle, a part of culture solution can be taken out without opening the bottle cap by a worker, so that the growth condition of cells in the culture solution can be observed, a large amount of impurity bacteria in the external environment are prevented from entering the culture bottle, and the cell culture bottle has the advantage of improving the cell culture effect.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a cell culture flask. Background Technology

[0002] Culture flasks are essential tools for the in vitro culture of cells and tissues. A typical cell culture flask consists of a body and a cap. The operator pours the culture medium containing cells into the body, then closes the cap, allowing the cells to be cultured in a sealed environment.

[0003] Cell growth varies with culture duration. To observe cell growth at different stages, staff need to periodically remove portions of the culture medium in batches and then use instruments to observe the cells within. The traditional method involves opening the bottle cap, pouring out a measured amount of culture medium, and then closing the cap. However, this method exposes the culture medium inside the bottle directly to the air, making it easy for airborne impurities and bacteria to enter the bottle during the removal process, affecting the subsequent growth of the cells in the remaining culture medium. Utility Model Content

[0004] In view of the above problems, this utility model provides a cell culture flask.

[0005] To achieve the above-mentioned objectives, the technical solution adopted by this utility model is as follows:

[0006] A cell culture flask is provided, including a flask body and a cap disposed on the flask body. A liquid dispensing tube is slidably disposed coaxially on the cap. One end of the liquid dispensing tube extends into the flask body and is connected to a piston on the outer wall of the tube section. The other end of the liquid dispensing tube extends out of the cap, bends downward and is connected to a sealing head. A liquid guide tube is provided at the bottom of the flask body for insertion and cooperation with the liquid dispensing tube. Multiple liquid guide holes are opened on the side wall of the liquid guide tube.

[0007] Furthermore, the sealing head includes a stop block, a baffle plate, and a connecting post. The connecting post is fixed between the stop block and the baffle plate. The opening at the end face of the liquid extraction tube extending out of the bottle cap is a non-circular opening. The connecting post is movably inserted through the non-circular opening. One end of the connecting post extends into the inside of the liquid extraction tube and is fixedly connected to the stop block. The other end of the connecting post extends out of the liquid extraction tube and is fixedly connected to the baffle plate. The liquid extraction tube is provided with an elastic element for driving the stop block to move upward and causing the baffle plate to seal the non-circular opening.

[0008] Furthermore, the elastic element includes a spring, one end of which is connected to the stop block, and the other end of which is connected to the inner bottom wall of the liquid extraction tube located at the irregular opening.

[0009] Further, the liquid guide pipe is arranged on the inner bottom wall of the bottle body, the outer wall of the liquid guide pipe is provided with stirring blades for stirring the culture solution, the inner side of the liquid guide pipe is provided with a flow channel with a special-shaped cross section, and the end of the liquid taking pipe away from the plug head is fixedly provided with a connecting head for plug-in cooperation with the flow channel.

[0010] Further, the stirring blades are made of flexible material, and one side of the stirring blades is in extrusion contact with the inner side wall of the bottle body.

[0011] Further, a handle is fixedly arranged on the outer wall of the pipe segment of the liquid taking pipe penetrating through the bottle cap.

[0012] The beneficial effects of the cell culture bottle are as follows: when the bottle cap is installed on the bottle body by the staff, the end of the liquid taking pipe is plug-in arranged at the top of the liquid guide pipe, in the liquid taking process, the plug head is opened and the liquid taking pipe is vertically moved downward, so that the piston is moved downward, and thus the culture solution can enter the liquid taking pipe along the liquid guide pipe under the action of atmospheric pressure, part of the culture solution is taken out, the plug head is closed after the liquid taking is completed, part of the culture solution can be taken out without opening the bottle cap by the staff, the cell growth in the culture solution is observed, a large amount of impurities and bacteria in the external environment are prevented from entering the culture bottle, and the cell culture effect is improved. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 It is a whole structure schematic view of the cell culture bottle.

[0014] Figure 2 It is an assembly schematic view of the bottle cap and the liquid taking pipe of the cell culture bottle.

[0015] Figure 3 It is an internal structure schematic view of the bottle body of the cell culture bottle.

[0016] Figure 4 It is a structure schematic view of the plug head of the cell culture bottle.

[0017] 1, bottle body; 2, bottle cap; 3, liquid taking pipe; 31, special-shaped port; 32, connecting head; 321, notch; 4, piston; 5, plug head; 51, stop block; 52, baffle; 53, connecting column; 6, liquid guide pipe; 61, liquid guide hole; 7, spring; 8, stirring blade; 9, handle. DETAILED DESCRIPTION

[0018] In order to better understand the above technical solutions, the above technical solutions will be described in detail in combination with the drawings of the specification and specific embodiments.

[0019] The cell culture bottle is disclosed in the embodiments of the application, which mainly comprises Figure 1 , Figure 2 andFigure 3 The cell culture bottle comprises a bottle body 1 and a bottle cap 2 threadedly connected to the bottle body 1, a liquid taking pipe 3 is coaxially arranged on the bottle cap 2, one end of the liquid taking pipe 3 extends into the bottle body 1 and is fixedly connected with a piston 4 on the outer wall of the pipe segment, the other end of the liquid taking pipe 3 extends out of the bottle cap 2, is bent downward and is connected with a blocking head 5, and a liquid guide pipe 6 for plugging cooperation with the liquid taking pipe 3 is arranged at the bottom of the bottle body 1, and a plurality of liquid guide holes 61 are arranged on the side wall of the liquid guide pipe 6.

[0020] In the embodiment of the present application, a through hole is arranged on the bottle cap 2 for the liquid taking pipe 3 to pass through, and the piston 4 is in extrusion contact with the inner side wall of the bottle body 1. When the staff installs the bottle cap 2 on the bottle body 1, the one end of the liquid taking pipe 3 can be plugged on the top of the liquid guide pipe 6, and in the process of taking liquid, the blocking head 5 is opened and the liquid taking pipe 3 is vertically moved downward, so that the piston 4 is moved downward, so that the culture solution can enter the liquid taking pipe 3 along the liquid guide pipe 6 under the action of atmospheric pressure, so as to take out part of the culture solution, and the blocking head 5 is closed after the liquid taking is completed. The cell culture bottle of the present application can take out part of the culture solution without opening the bottle cap 2 by the staff, so as to observe the growth of cells in the culture solution, avoid a large amount of impurity bacteria in the external environment from entering the culture bottle, and has the advantage of improving the cell culture effect.

[0021] Reference Figure 4 The blocking head 5 comprises a stop block 51, a baffle 52 and a connecting column 53, the connecting column 53 is fixedly arranged between the stop block 51 and the baffle 52, the opening at the end face of the one end of the liquid taking pipe 3 extending out of the bottle cap 2 is an irregular-shaped opening 31, and the connecting column 53 is movably arranged at the irregular-shaped opening 31. One end of the connecting column 53 extends into the inner side of the liquid taking pipe 3 and is welded and fixed with the stop block 51, the other end of the connecting column 53 extends out of the liquid taking pipe 3 and is welded and fixed with the baffle 52, and the liquid taking pipe 3 is provided with an elastic member for driving the stop block 51 to move upward and making the baffle 52 block the irregular-shaped opening 31.

[0022] In the embodiment of the present application, when the staff moves the liquid taking pipe 3 downward and makes the culture solution enter the liquid taking pipe 3, the baffle 52 can be moved downward under the action of pressure and overcome the elastic force of the elastic member, so that the culture solution can be discharged from the irregular-shaped opening 31. After the liquid taking is completed, the staff releases the liquid taking pipe 3, the stop block 51 moves upward under the action of the elastic force of the elastic member, so that the baffle 52 blocks the irregular-shaped opening 31, and the convenience of the liquid taking work is further improved.

[0023] Specifically, the elastic member comprises a spring 7, one end of the spring 7 is connected with the stop block 51, and the other end of the spring 7 is connected with the inner bottom wall of the end portion of the liquid taking pipe 3 at the irregular-shaped opening 31.

[0024] In the embodiment of the present application, the two ends of the spring 7 are respectively in abutment with the stop block 51 and the inner bottom wall of the end portion of the liquid taking pipe 3 at the irregular-shaped opening 31.

[0025] Further, the liquid guide pipe 6 is rotatably arranged on the inner bottom wall of the bottle body 1, and the outer wall of the liquid guide pipe 6 is provided with stirring blades 8 for stirring the culture solution, the inner side of the liquid guide pipe 6 is provided with a flow channel with a special cross section, and the end of the liquid taking pipe 3 away from the plug head 5 is integrally provided with a connecting head 32 for plugging with the flow channel, and a plurality of notches 321 are formed in the side wall of the connecting head 32.

[0026] In the embodiment of the present application, the liquid taking pipe 3 is a cylindrical pipe, so the liquid taking pipe 3 is arranged in the through hole, which can not only coaxially slide relative to the bottle cap 2, but also rotate. After the culture solution is poured into the bottle body 1 and the bottle cap 2 is covered, the liquid taking pipe 3 is rotated, so that the liquid guide pipe 6 is rotated, and the stirring blades 8 can uniformly stir the culture solution.

[0027] Further, the stirring blades 8 are made of flexible material, and one side of the stirring blades 8 is in extrusion contact with the inner side wall of the bottle body 1.

[0028] In the embodiment of the present application, the stirring blades 8 can be made of rubber. One side of the stirring blades 8 is in extrusion contact with the inner side wall of the bottle body 1, which can ensure that the culture solution adhered to the inner side wall of the bottle body 1 can also be uniformly stirred.

[0029] In order to facilitate the staff to move the liquid taking pipe 3, a handle 9 is integrally arranged on the outer wall of the pipe segment of the liquid taking pipe 3.

[0030] The implementation principle of the cell culture bottle in the embodiment of the present application is as follows: when the staff installs the bottle cap 2 on the bottle body 1, the end of the liquid taking pipe 3 is plugged on the top of the liquid guide pipe 6, in the process of taking liquid, the plug head 5 is opened and the liquid taking pipe 3 is vertically and downwardly moved, so that the piston 4 is downwardly moved, and the culture solution can enter the liquid taking pipe 3 along the liquid guide pipe 6 under the action of atmospheric pressure, so as to take out part of the culture solution, and the plug head 5 is closed after the liquid taking is completed. The cell culture bottle of the present application can take out part of the culture solution without opening the bottle cap 2, so as to observe the growth of cells in the culture solution, which avoids a large amount of impurities and bacteria in the external environment from entering the culture bottle, and has the advantage of improving the cell culture effect.

[0031] Those skilled in the art will appreciate that although the preferred embodiments of the present application have been described, once the basic creative concept is known, those skilled in the art can make other changes and modifications to the embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications falling within the scope of the present application. Obviously, those skilled in the art can make various modifications and changes to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and changes of the present application fall within the scope of the claims of the present application and their equivalents, the present application also intends to include these modifications and changes.

Claims

1. A cell culture flask, characterized by: The utility model provides bottle body (1) and the bottle cover (2) of setting on bottle body (1), coaxially sliding setting has the liquid taking pipe (3) on the bottle cover (2), one end of the liquid taking pipe (3) extends into bottle body (1) and is connected with piston (4) on the outer wall of pipe section, the other end of the liquid taking pipe (3) extends out the bottle cover (2) and is connected with the obturator (5) after bending down, the bottom of bottle body (1) is equipped with the liquid guide pipe (6) for with the plug -in cooperation of liquid taking pipe (3), a plurality of liquid guide holes (61) are set up on the lateral wall of liquid guide pipe (6).

2. The cell culture flask of claim 1, wherein: The obturator (5) includes a stop block (51), a baffle (52), and a connecting column (53), the connecting column (53) is fixed between the stop block (51) and the baffle (52), the opening at the end face of the one end of the liquid taking pipe (3) extending out of the bottle cover (2) is set as a special-shaped opening (31), the connecting column (53) is movably arranged at the special-shaped opening (31), one end of the connecting column (53) extends into the inside of the liquid taking pipe (3) and is fixedly connected with the stop block (51), the other end of the connecting column (53) extends out of the liquid taking pipe (3) and is fixedly connected with the baffle (52), and the liquid taking pipe (3) is provided with an elastic member for driving the stop block (51) to move upwards and making the baffle (52) block the special-shaped opening (31).

3. The cell culture flask of claim 2, wherein: The elastic member includes a spring (7), one end of the spring (7) is connected with the stop block (51), and the other end of the spring (7) is connected with the inner bottom wall of the end of the liquid taking pipe (3) at the special-shaped opening (31).

4. The cell culture flask of claim 1, wherein: The liquid guide pipe (6) is rotatably arranged on the inner bottom wall of the bottle body (1), the outer wall of the liquid guide pipe (6) is provided with stirring blades (8) for stirring the culture solution, the inside of the liquid guide pipe (6) is provided with a flow channel with a special-shaped cross section, one end of the liquid taking pipe (3) away from the obturator (5) is fixedly provided with a connecting head (32) for plug-in cooperation with the flow channel, and a plurality of notches (321) are formed in the side wall of the connecting head (32).

5. The cell culture flask of claim 4, wherein: The stirring blades (8) are made of flexible material, and one side of the stirring blades (8) is in extrusion contact with the inner side wall of the bottle body (1).

6. The cell culture flask of claim 4, wherein: A handle (9) is fixedly arranged on the outer wall of the pipe section of the liquid taking pipe (3) extending out of the bottle cover (2).

Images