Sample movement assembly for living cell imager

Abstract

The utility model relates to the technical field of living cell observation, in particular to a sample movement assembly for a living cell imager. A sample movement assembly for a living cell imager comprises an X-axis linear movement assembly used for driving a sample clamp to move in the X-axis direction and a Y-axis linear movement assembly used for driving the X-axis movement assembly to move in the Y-axis direction. The X-axis linear motion assembly is connected with a sliding block of the Y-axis linear motion assembly through a mounting seat, and the two assemblies are arranged in an orthogonally overlapped manner, so that a sample can move in a two-dimensional plane; one end of the mounting base is fixed to a sliding block of the Y-axis linear movement assembly, the other end of the mounting base makes contact with an external objective table through the adjustable supporting assembly, a simply supported beam structure with the two ends rigidly supported is formed and used for avoiding movement precision loss caused by cantilever deformation, and the adjustable supporting assembly eliminates cantilever deformation and guarantees movement stability. Therefore, accurate movement of the sample is realized, and the device is adaptive to a living cell imager.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of living cell observation, specifically relates to a sample motion subassembly for living cell imaging appearance. BACKGROUND

[0002] In the field of living cell observation, the living cell imaging appearance is one of the commonly used equipment, is used for real -time observation and record to living cell's form, structure and physiological activity etc. However, the existing fixed stage living cell imaging appearance has certain limitation, especially in sample movement: first, the fixed stage equipment depends on manual direct movement sample, is difficult to aim when operating and is easy to cause millimeter level displacement deviation, difficult to reach high -precision movement requirement, there are microscopic field loss or image sequence misalignment etc. Second, the conventional retrofit scheme needs to cut the microscope platform and install the electric displacement table, destroys the original equipment airtightness and is high in cost; Third, the general type microscope displacement table on the market will shield the light path of the condenser lens, and the table surface has centimeter level thickness, which exceeds the millimeter level focal length of the living cell imaging appearance, and cannot be adapted.

[0003] Therefore, it is urgent to provide an accurate and fine sample movement assembly for the existing living cell imaging appearance without changing the appearance of the living cell imaging appearance. CONTENT OF THE UTILITY MODEL

[0004] The utility model provides a sample motion subassembly for living cell imaging appearance for the technical problems existing in the prior art, realizes the movement of the sample and guarantees accuracy and delicacy.

[0005] The technical scheme for solving the above technical problems of the utility model is as follows:

[0006] A sample motion subassembly for living cell imaging appearance, comprising

[0007] The X-axis linear motion assembly is used for driving the sample clamp to move along the X-axis direction;

[0008] The Y-axis linear motion assembly is used for driving the X-axis motion assembly to move along the Y-axis direction;

[0009] The X-axis linear motion assembly is connected with the slider of the Y-axis linear motion assembly through the mounting seat, and the two assemblies are orthogonally stacked;

[0010] One end of the mounting seat is fixed to the slider of the Y-axis linear motion assembly, and the other end is in contact with the external stage through the adjustable support assembly.

[0011] On the basis of the above technical scheme, the utility model can also be improved as follows.

[0012] Further, the adjustable support assembly comprises a limiting screw, a ball bearing pulley, a gasket and a mounting bracket;

[0013] The mounting bracket is connected to one end of the mounting base and has a screw hole inside;

[0014] The upper end of the limiting screw is connected to the mounting bracket through the screw hole, and the lower end of the limiting screw is provided with a limiting hole;

[0015] The ball bearing pulley is installed in the limiting hole and can rotate freely, and the lower end of the ball bearing pulley is in contact with the external sample stage;

[0016] The gasket is located between the limiting screw and the screw hole.

[0017] Further, the mounting base comprises a U-shaped mounting frame and an L-shaped mounting frame, the U-shaped mounting frame is composed of a bottom plate and two side plates, and the two ends of the bottom plate are respectively connected to a side plate vertically; the L-shaped mounting frame is composed of a horizontal plate and a longitudinal plate connected to each other vertically, the upper end of the longitudinal plate is connected to the lower end of the U-shaped mounting frame, and the lower end of the longitudinal plate is connected to the horizontal plate.

[0018] Further, the X-axis linear motion assembly comprises a lead screw, a first guide rail, a first hand wheel and a nut seat;

[0019] The first guide rail is horizontally installed on the bottom plate, the lead screw is connected to the two side plates of the mounting base through bearings at the two axial ends respectively, the nut seat is threadedly connected to the lead screw, the lower end of the nut seat is provided with a sliding groove matched with the first guide rail and is in sliding connection with the first guide rail, the nut seat is connected to a sample clamp, and the first hand wheel is located on one side of the mounting base and is fixedly connected to the lead screw to drive the lead screw to rotate.

[0020] Further, the X-axis linear motion assembly further comprises a protective cover, and the surface of the protective cover is provided with a scale.

[0021] Further, the Y-axis linear motion assembly comprises an inclined rack, an inclined gear, a second hand wheel, a second guide rail, a sliding block and a mounting plate;

[0022] The second guide rail is installed on the surface of the mounting plate, the inclined rack is installed on the surface of the second guide rail, the inclined gear is in engagement with the inclined rack, the sliding block is provided with a sliding groove matched with the second guide rail and is in sliding connection with the second guide rail, the sliding block is provided with a through hole, and the second hand wheel is connected to the inclined gear through a set screw passing through the through hole and drives the inclined gear to rotate.

[0023] Further, the material of the inclined rack is copper or stainless steel.

[0024] Further, the modulus of the inclined rack and the inclined gear is 0.5 module, and the number of teeth of the inclined gear is 15-17 teeth.

[0025] Further, an adjustable gap adjusting gasket is arranged between the contact surfaces of the inclined gear and the second hand wheel.

[0026] Further, the horizontal plate upper surface is provided with the sliding block, and the mounting plate lower surface is provided with the second guide rail, and the sliding block is in sliding connection with the second guide rail.

[0027] Further, the stroke ratio of the Y-axis linear motion assembly and the X-axis linear motion assembly is set as 1:1 to 1:2, the maximum stroke range of the X-axis is 140-160 mm, and the maximum stroke range of the Y-axis is 80-160 mm.

[0028] The utility model discloses the beneficial effects are:

[0029] 1, the mounting seat one end is fixed in the sliding block of Y-axis linear motion assembly, and the other end is contacted with external object carrier through adjustable support component, forms the simply supported beam structure of two end rigid support, adjusts X-axis linear motion assembly with object carrier parallel through adjustable support component, and makes sample clamp hang in the air, keeps the interval of sample clamp and object carrier 1mm-2mm, and simply supported beam structure can avoid the gravity deviation of cantilever beam structure under because sample moves, leads to the slight bending of X-axis linear motion assembly, thereby avoids sample clamp and object carrier collision and focal length change.

[0030] 2, the utility model discloses X-axis linear motion assembly and Y-axis linear motion assembly adopt the orthogonal laminated arrangement, and X-axis linear motion assembly adopts screw guide rail transmission, and Y-axis linear motion assembly adopts gear and rack transmission, realizes the accurate movement of sample clamp in two-dimensional plane. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 It is the structure schematic view of sample motion assembly for the utility model embodiment;

[0032] Figure 2 It is the structure schematic view of X-axis linear motion assembly for the utility model embodiment;

[0033] Figure 3 It is the structure schematic view of Y-axis linear motion assembly for the utility model embodiment.

[0034] In the drawings, the component list represented by each sign is as follows:

[0035] 2, Y-axis linear motion assembly, 21, slider, 22, helical rack, 23, mounting plate, 24, gap adjusting washer, 25, second hand wheel, 26, second guide rail, 3, X-axis linear motion assembly, 31, screw rod, 32, nut seat, 33, first guide rail, 34, bearing, 35, first hand wheel, 36, mounting seat, 361, bottom plate, 362, side plate, 363, horizontal plate, 364, longitudinal plate, 365, scale, 366 protective cover, 37, mounting bracket, 38, washer, 39, limit screw, 4, sample clamp. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0037] In the description of the present application, the terms "first", "second" are used only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0038] In the description of the present application, the term "for example" is used to indicate "as an example, illustration or description". Any embodiment described as "for example" in the present application is not necessarily interpreted as more preferred or more advantageous than other embodiments. The following description is given in order to enable any person skilled in the art to implement and use the present application. In the following description, details are listed for the purpose of explanation. It should be understood that those skilled in the art can realize the present application without using these specific details. In other examples, well-known structures and processes will not be described in detail to avoid unnecessary details making the description of the present application obscure. Therefore, the present application is not intended to be limited to the shown embodiments, but is consistent with the broadest scope of principles and features disclosed in the present application.

[0039] Embodiments

[0040] As Figures 1-2As shown, a sample motion assembly for a live cell imager includes an X-axis linear motion assembly 3 for driving a sample holder 4 to move along an X-axis direction and a Y-axis linear motion assembly 2 for driving the X-axis linear motion assembly 3 to move along a Y-axis direction; the X-axis linear motion assembly 3 is connected with a slider 21 of the Y-axis linear motion assembly 2 through a mounting seat 36, the two assemblies are orthogonally stacked to realize the movement of the sample in a two-dimensional plane; one end of the mounting seat 36 is fixed to the slider 21 of the Y-axis linear motion assembly 2, and the other end is in contact with an external stage through an adjustable support assembly, forming a simply supported beam structure with rigid support at both ends, which is used to avoid the loss of movement accuracy caused by cantilever deformation, and the adjustable support assembly eliminates the cantilever deformation to ensure the stability of the movement.

[0041] Specifically, the adjustable support assembly includes a limiting screw 39, a ball pulley, a gasket 38 and a mounting bracket 37; the mounting bracket 37 is connected to one end of the mounting seat 36 and has a screw hole inside; the limiting screw 39 is connected to the mounting bracket 37 through the screw hole at the upper end and has a limiting hole at the lower end; the ball pulley is installed in the limiting hole and can rotate freely, and the lower end of the ball pulley is in contact with the external stage; the gasket 38 is located between the limiting screw 39 and the screw hole. A certain number of gaskets 38 with a thickness of 0.1 mm are used to adjust the parallelism between the X-axis linear motion assembly 3 and the stage, and the sample holder 4 is suspended, keeping the distance between the sample holder 4 and the stage at 1-2 mm; the simply supported beam structure can avoid the displacement of the center of gravity caused by the movement of the sample under the cantilever beam structure, which causes the X-axis linear motion assembly 3 to bend slightly, thereby avoiding the collision between the sample holder 4 and the stage and the change of focal length.

[0042] In a preferred embodiment, the mounting seat 36 includes a U-shaped mounting frame and an L-shaped mounting frame; the U-shaped mounting frame is composed of a bottom plate 361 and two side plates 362, and the two ends of the bottom plate 361 are respectively connected with a side plate 362 perpendicularly; the L-shaped mounting frame is composed of a horizontal plate 363 and a longitudinal plate 364 connected perpendicularly, and the upper end of the longitudinal plate 364 is connected with the lower end of the U-shaped mounting frame, and the lower end is connected with the horizontal plate 363.

[0043] In a preferred embodiment, the X-axis linear motion component 3 includes a lead screw 31, a first guide rail 33, a first handwheel 35, a nut seat 32, and a protective cover 366. The first guide rail 33 is horizontally mounted on the base plate 361. The two ends of the lead screw 31 are connected axially to the two side plates 362 of the mounting base 36 via bearings 34. The nut seat 32 is threadedly connected to the lead screw 31, and its lower end has a groove that matches and slidably connects to the first guide rail 33. The nut seat 32 is connected to the sample clamp 4 via a connector. The first handwheel 35 is located on one side of the mounting base 36 and is fixedly connected to the lead screw 31, driving the lead screw 31 to rotate. The protective cover 366 has a scale 365 on its surface. The lead screw and guide rail transmission mechanism provides high-precision linear movement, the first handwheel 35 facilitates fine-tuning, and the first guide rail 33 provides guidance for the nut seat 32. Rotating the first handwheel 35 drives the sample clamp 4 to move along the X-axis.

[0044] like Figure 3 As shown, in a preferred embodiment, the Y-axis linear motion component 2 includes a helical rack 22, a helical gear, a second handwheel 25, a second guide rail 26, a slider 21, and a mounting plate 23. The second guide rail 26 is mounted on the surface of the mounting plate 23, and the helical rack 22 is mounted on the surface of the second guide rail 26. The helical gear meshes with the helical rack 22. The slider 21 has a groove that matches the second guide rail 26 and is slidably connected to it. The slider 21 has a through hole, and the second handwheel 25 is connected to the helical gear through the through hole via a set screw and drives its rotation. High-precision linear movement is achieved using rack and pinion transmission. Rotating the second handwheel 25 drives the slider 21 to move along the Y-axis. The X-axis linear motion component 3 is connected to the slider 21 of the Y-axis linear motion component 2 via a mounting base 36. The movement of the slider 21 drives the movement of the Y-axis linear motion component 2.

[0045] The slider 21 is mounted on the upper surface of the horizontal plate 363, and the second guide rail 26 is mounted on the lower surface of the mounting plate 23. The slider 21 is slidably connected to the second guide rail 26. The inverted guide rail and slider 21 design reduces the overall height and avoids interference with the optical path of the microscope.

[0046] Specifically, the helical rack 22 is made of copper, which is not easily corroded.

[0047] Specifically, the helical rack 22 and the helical gear have a module of 0.5, and the helical gear has 15-17 teeth.

[0048] Specifically, an adjustable gap adjustment shim 24 is provided between the contact surface of the helical gear and the second handwheel 25.

[0049] The working process of this utility model is as follows: the ball pulley of the adjustable support component is placed on the stage, the number of shims 38 is adjusted to make the X-axis component 3 parallel to the stage, and the sample clamp 4 is suspended in the air, keeping the distance between the sample clamp 4 and the stage at 1mm-2mm. The sample clamp 4 is driven to move along the X-axis direction by rotating the first handwheel 35, and the sample clamp 4 is driven to move along the Y-axis direction by rotating the second handwheel 25, thereby realizing the precise movement of the sample clamp 4 in the two-dimensional plane.

[0050] In summary, by orthogonally stacking the X-axis linear motion component 3 and the Y-axis linear motion component 2, along with a simply supported beam design, and utilizing lead screw and rack / pinion transmission, precise sample positioning under manual drive is achieved while ensuring stability, making it compatible with live-cell imaging instruments. This invention achieves precise sample positioning through the diagonal thrust of the spring clip 41 and the reference plane within the positioning frame 41, ensuring no sample offset. Different samples can be adapted by replacing the transition groove 412 or the perforated plate, and the overall hollowed-out bottom design effectively prevents interference microscope focusing.

[0051] While embodiments or examples of this disclosure have been described with reference to the accompanying drawings, it should be understood that the methods, systems, and devices described above are merely exemplary embodiments or examples, and the scope of this utility model is not limited by these embodiments or examples, but only by the granted claims and their equivalents. Various elements in the embodiments or examples may be omitted or replaced by their equivalents. Furthermore, the steps may be performed in a different order than that described in this disclosure. Further, various elements in the embodiments or examples may be combined in various ways. Importantly, as technology evolves, many elements described herein can be replaced by equivalents that appear after this disclosure.

Claims

1. A sample motion assembly for a live cell imager, comprising: The utility model relates to a sample holder linear motion mechanism for electron microscope X-axis linear motion component for driving sample clamp along X-axis direction movement; Y-axis linear motion component for driving X-axis motion component along Y-axis direction movement; The X-axis linear motion component is connected with the slider of Y-axis linear motion component through the mounting base, and the two components are orthogonally laminated; One end of the mounting base is fixed to the slider of Y-axis linear motion component, and the other end is connected with the external stage through the adjustable support component.

2. The sample motion assembly for a live cell imager of claim 1, wherein, The adjustable support component includes a limiting screw, a ball bearing pulley, a gasket and a mounting bracket; The mounting bracket is connected to one end of the mounting base and has a screw hole inside; The limiting screw is connected to the mounting bracket through the screw hole, and the lower end of the limiting screw has a limiting hole; The ball bearing pulley is installed in the limiting hole and can rotate freely, and the lower end of the ball bearing pulley is in contact with the external stage; The gasket is located between the limiting screw and the screw hole.

3. The sample motion assembly for a live cell imager of claim 1, wherein, The mounting base includes a U-shaped mounting frame and an L-shaped mounting frame, the U-shaped mounting frame is composed of a bottom plate and two side plates, one side plate is vertically connected to each end of the bottom plate;The L-shaped mounting frame is composed of a horizontal plate and a longitudinal plate connected perpendicularly, the upper end of the longitudinal plate is connected to the lower end of the U-shaped mounting frame, and the lower end is connected to the horizontal plate.

4. The sample motion assembly for a live cell imager of claim 3, wherein, The X-axis linear motion component includes a lead screw, a first guide rail, a first hand wheel and a nut seat; The first guide rail is horizontally installed on the bottom plate, the lead screw is connected to the two side plates of the mounting base through bearings at both ends, the nut seat is threadedly connected to the lead screw, a sliding groove matched with the first guide rail is formed in the lower end of the nut seat, the nut seat is connected to the sample holder, and the first hand wheel is located on one side of the mounting base and is fixedly connected to the lead screw to drive the rotation of the lead screw.

5. The sample motion assembly for a live cell imager of claim 4, wherein, The X-axis linear motion component further includes a protective cover, and the surface of the protective cover is provided with a scale.

6. The sample motion assembly for a live cell imager of claim 3, wherein, The Y-axis linear motion component includes a helical rack, a helical gear, a second hand wheel, a second guide rail, a slider and a mounting plate; The second guide rail is installed on the surface of the mounting plate, the helical rack is installed on the surface of the second guide rail, the helical gear is engaged with the helical rack, the slider has a sliding groove matched with the second guide rail and is slidably connected with the second guide rail, the slider has a through hole, and the second hand wheel is connected to the helical gear through a set screw and drives the rotation of the helical gear.

7. The sample motion assembly for a live cell imager of claim 6, wherein, The material of the helical rack is copper or stainless steel.

8. The sample motion assembly for a live cell imager of claim 6, wherein, The modulus of the helical rack and the helical gear is 0.5 module, and the number of teeth of the helical gear is 15-17 teeth.

9. The sample motion assembly for a live cell imager of claim 6, wherein, An adjustable gap adjusting gasket is arranged between the contact surfaces of the helical gear and the second hand wheel.

10. The sample motion assembly for a live cell imager of claim 6, wherein, The slider is slidably connected with the second guide rail.

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