Environment DNA enrichment device applied to water body

Abstract

The utility model relates to an environmental DNA (deoxyribonucleic acid) enrichment device applied to water, which belongs to the field of water sampling equipment and comprises a water suction pump and a water suction pipe communicated with the water suction pump, and further comprises a water delivery pipe and an enrichment component arranged between the water suction pipe and the water delivery pipe. The two sides of the coating film are communicated with butt joint pipes, butt joint sleeves are arranged at ports of the butt joint pipes, detachable sealing plugs are arranged in the butt joint sleeves, the filter membrane comprises an edge pressing area and a filter area, a tearing line is arranged between the edge pressing area and the filter area, the coating film is of a two-section structure and clamps the edge pressing area of the filter membrane from the two sides, a water inlet pipe is arranged at the water inlet end of the water conveying pipe, and a water outlet pipe is arranged at the water outlet end of the water conveying pipe. A water inlet cover is arranged on the water inlet pipe; and a filter screen is arranged at a water inlet of the water inlet cover. According to the utility model, the filter membrane, the coating membrane, the butt joint pipe and the butt joint sleeve are matched, so that the enrichment assembly is endowed with detachable and foldable functions, and the convenience and the operation efficiency of collecting the environmental DNA of the water body are effectively improved.

Description

Technical Field

[0001] This utility model belongs to the field of water sampling equipment, specifically relating to an environmental DNA enrichment device for use in water bodies. Background Technology

[0002] Existing methods for enriching environmental DNA in water involve filtering water using specialized membranes. After filtering a sufficient volume of water, the membrane accumulates a relatively comprehensive amount of environmental DNA. However, this process requires outdoor collection, necessitating laboratory testing of the membrane. To prevent DNA loss, cryovials and associated cryopreservation equipment are also required. Furthermore, the membranes are often small, limiting filtration efficiency and leading to lengthy collection times. To prevent contamination, existing environmental DNA enrichment devices for water use are equipped with detachable filter elements to isolate the membrane from the external environment. After collection, the filter element must be opened and the membrane removed on-site, posing a high risk of contamination. To address these issues, this invention proposes an environmental DNA enrichment device for water. Utility Model Content

[0003] The purpose of this invention is to provide an environmental DNA enrichment device for use in water bodies in order to solve the above-mentioned problems.

[0004] This utility model achieves the above objectives through the following technical solutions:

[0005] This utility model provides an environmental DNA enrichment device for use in water bodies, including a water pump and a water pumping pipe connected to the water pump, as well as a water delivery pipe and an enrichment component disposed between the water pumping pipe and the water delivery pipe.

[0006] The enrichment component includes a filter membrane, a coating membrane is sleeved on the outside of the filter membrane, and connecting pipes are connected to both sides of the coating membrane. A connecting sleeve is provided at the port of the connecting pipe, and a removable sealing plug is provided inside the connecting sleeve.

[0007] As a further optimization of this utility model, the filter membrane includes a pressing area and a filtering area, and a tear line is provided between the pressing area and the filtering area.

[0008] As a further optimization of this utility model, the coating membrane adopts a two-lobed structure and clamps the edge area of ​​the filter membrane from both sides.

[0009] As a further optimization of this utility model, the water inlet end of the water supply pipe is provided with a water inlet pipe, the water inlet pipe is provided with a water inlet cover, and the water inlet of the water inlet cover is provided with a filter screen.

[0010] As a further optimization of this utility model, a retainer is provided on the outer side of the water inlet cover, and the part of the retainer to be engaged has a missing ring structure.

[0011] As a further optimization of this utility model, the connecting sleeve is connected to the ports of the pumping pipe and the water supply pipe by threaded engagement.

[0012] The beneficial effects of this utility model are as follows:

[0013] This environmental DNA enrichment device for water bodies employs an enrichment assembly composed of a filter membrane, a coating membrane, a connecting pipe, and a docking sleeve. The docking sleeve, in conjunction with the pumping and supply pipes, enables the enrichment assembly to be easily assembled and disassembled. The filter membrane, coating membrane, and connecting pipe within the enrichment assembly are all made of flexible materials, allowing for easy folding. The folded assembly is compact and can be stored entirely in a cryogenic storage device, avoiding contamination issues caused by removing the filter membrane on-site. Furthermore, the enrichment assembly provides ample filtration space when unfolded, effectively improving filtration efficiency compared to existing small-sized filters, thereby enhancing the collection efficiency of environmental DNA from the water and solving the problems mentioned in the background technology. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall appearance of this utility model;

[0015] Figure 2 This is a schematic diagram showing the disassembled structure of this utility model;

[0016] Figure 3 This is a cross-sectional view of the enriched components;

[0017] Figure 4 This is a schematic diagram of a filter membrane;

[0018] Figure 5 This is a diagram showing the fit of the water inlet pipe, water inlet cover, and clamp.

[0019] In the diagram: 1. Water pump; 2. Pumping pipe; 3. Enrichment module; 301. Filter membrane; 3011. Edge pressing area; 3012. Filtration area; 3013. Tear line; 302. Coating membrane; 303. Connecting pipe; 304. Connecting sleeve; 305. Sealing plug; 4. Water supply pipe; 5. Inlet pipe; 6. Inlet cover; 7. Compression sleeve. Detailed Implementation

[0020] The present application will now be described in further detail. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0021] Example 1

[0022] like Figure 1-5 As shown, an environmental DNA enrichment device for water bodies according to this embodiment includes a water pump 1 and a water pumping pipe 2 connected to the water pump 1, and also includes a water delivery pipe 4 and an enrichment component 3 disposed between the water pumping pipe 2 and the water delivery pipe 4.

[0023] The enrichment component 3 includes a filter membrane 301, a covering membrane 302 is sleeved on the outside of the filter membrane 301, and a connecting pipe 303 is connected to both sides of the covering membrane 302. A connecting sleeve 304 is provided at the port of the connecting pipe 303, and a detachable sealing plug 305 is provided inside the connecting sleeve 304. The connecting sleeve 304 is connected to the port of the pumping pipe 2 and the water supply pipe 4 by threaded engagement.

[0024] The covering membrane 302 and the connecting pipe 303 are both made of thin film material, the connecting sleeve 304 is made of hard plastic or silicone material, the sealing plug 305 is made of silicone material, and the water pumping pipe 2 and the water supply pipe 4 are both flexible hoses.

[0025] Before and after collection, the enrichment component 3 can be folded and stored. Specifically, the coating membrane 302 and the connecting tube 303 are flattened and then rolled up. This storage method can reduce the volume of the enrichment component 3 itself, and also prevent the filter membrane 301 inside the enrichment component 3 from being contaminated. Moreover, when the enrichment component 3 is fully unfolded, it can provide a larger filtration space, which is conducive to improving the water filtration efficiency and thus improving the enrichment efficiency of environmental DNA in the water.

[0026] The specific usage method is as follows: Before collection, first take out the enrichment component 3 and flatten it. After taking out the sealing plug 305 (using tools such as tweezers), screw the two docking sleeves 304 on the enrichment component 3 onto the ports of the pumping pipe 2 and the water supply pipe 4 respectively. Then, place the end of the water supply pipe 4 underwater and close to the water surface, and then turn on the pumping pump 1. Under the action of the pumping pump 1, the water flows through the water supply pipe 4, the enrichment component 3, the pumping pipe 3 and the pumping pump 1 in sequence and is discharged. After a large amount of water flows through the filter membrane 301 in the enrichment component 2, a sufficient amount of aquatic environmental DNA can be collected on the filter membrane 301. After completion, remove the enrichment component 3 by rotating the docking sleeve 304, squeeze out the water remaining in the enrichment component 3, and put the sealing plug 305 back in. Finally, roll up and fold the enrichment component 3, and place the folded enrichment component 3 in the cryopreservation equipment. After bringing the enrichment component 3 to the laboratory, break the filter membrane 301 and take out the filter membrane 301 and extract the environmental DNA from it.

[0027] Preferably, the filter membrane 301 includes a pressing area 3011 and a filtering area 3012, and a tear line 3013 is provided between the pressing area 3011 and the filtering area 3012;

[0028] The coating membrane 302 adopts a two-part structure and clamps the edge pressing area 3011 of the filter membrane 301 from both sides;

[0029] The pressing and pressing area 3011 of the coating membrane 302 serves to fix the filter membrane 301, which can prevent the filter membrane 301 from folding or curling under water impact, thus affecting the problem of insufficient collection of environmental DNA. When removing the filter membrane 301, an opening can be made directly on the outer edge of the coating membrane 302, and then one side of the coating membrane 302 can be lifted and the filter area 3012 can be torn off along the tear line 3013. The operation is convenient and efficient.

[0030] Preferably, the water inlet end of the water supply pipe 4 is provided with a water inlet pipe 5, the water inlet pipe 5 is provided with a water inlet cover 6, and the water inlet of the water inlet cover 6 is provided with a filter screen. The filter screen can block solid impurities in the water and reduce interference with the environmental DNA collected by the filter membrane 301.

[0031] Preferably, a retaining sleeve 7 is provided on the outer side of the water inlet cover 6. The part of the retaining sleeve 7 to be snapped is a ring-shaped structure. The retaining sleeve 7 can be used with the existing telescopic rod. After the water inlet pipe 5 and the water inlet cover 6 are connected and snapped onto the end of the telescopic rod, long-distance water pumping can be performed, making the operation more convenient and flexible.

[0032] The embodiments described above are merely examples of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these modifications and improvements all fall within the protection scope of this utility model.

Claims

1. An environmental DNA enrichment device applied to a water body, comprising a water suction pump (1) and a water suction pipe (2) in communication with the water suction pump (1), characterized in that, The water delivery pipe (4) and the enrichment assembly (3) arranged between the water pumping pipe (2) and the water delivery pipe (4) are further included. The enrichment assembly (3) comprises a filter membrane (301), the outer side of the filter membrane (301) is sleeved with a cladding film (302), both sides of the cladding film (302) are communicated with a butt joint pipe (303), a butt joint sleeve (304) is arranged at the port of the butt joint pipe (303), and a detachable sealing plug (305) is arranged in the butt joint sleeve (304).

2. The environmental DNA enrichment device for application to a water body according to claim 1, wherein, The filter membrane (301) comprises a pressing edge area (3011) and a filtering area (3012), and a tear line (3013) is arranged between the pressing edge area (3011) and the filtering area (3012).

3. The environmental DNA enrichment device for application to a water body according to claim 2, wherein, The cladding film (302) adopts a two-limb structure and clamps the pressing edge area (3011) of the filter membrane (301) from both sides.

4. The environmental DNA enrichment device for application to a water body according to claim 1, wherein, The water inlet end of the water delivery pipe (4) is provided with a water inlet pipe (5), the water inlet pipe (5) is provided with a water inlet cover (6), and the water inlet opening of the water inlet cover (6) is provided with a filter screen.

5. The environmental DNA enrichment device for application to a water body according to claim 4, wherein, The outer side of the water inlet cover (6) is sleeved with a clamping sleeve (7), and the part to be clamped on the clamping sleeve (7) is in a ring-shaped structure.

6. The environmental DNA enrichment device for application to a water body according to claim 4, wherein, The butt joint sleeve (304) is connected with the ports of the water pumping pipe (2) and the water delivery pipe (4) through thread rotation.

Images