Concentration and purification equipment for extracting protein from microalgae

Abstract

The utility model relates to the technical field of microalgae protein extraction, in particular to concentration and purification equipment for extracting protein from microalgae, which performs reflux extraction through a reflux extraction device and heats and evaporates mixed liquid in the reflux extraction device through an evaporation device. A mixed solution in the reflux extraction device is stirred through the stirring device, a mixed solution in the reflux extraction device is temporarily stored through the extraction device, the solution in the extraction device is extracted through the extraction device, the mixed solution is dialyzed through the dialysis device, and the mixed solution is freeze-dried through the freeze-drying device; comprising a console; the device further comprises a reflux extraction device, an evaporation device, a stirring device, an extraction device, an extraction device, a dialysis device and a freeze drying device, the reflux extraction device, the evaporation device, the extraction device, the extraction device, the dialysis device and the freeze drying device are all installed on the operation table, and the stirring device is installed on the reflux extraction device.

Description

Technical Field

[0001] This utility model relates to the technical field of microalgae protein extraction, and in particular to a concentration and purification device for extracting proteins from microalgae. Background Technology

[0002] Microalgae are rich in protein, easy to produce, and competitive with other protein sources. Therefore, this equipment can be used to produce microalgae proteins as key ingredients in functional foods, such as alternative protein sources, functional food components, food additives, and flavor enhancers.

[0003] For example, in a prior art represented by application number CN201922383227.X, the main structure includes a fixed frame, an extraction device with a feed pipe on one side of the top, and a freeze-drying device located on one side of the extraction device. A fixed plate is fixedly connected to one side of the top of the fixed frame via a first fixed rod and a second fixed rod. This concentration and purification equipment for extracting proteins from microalgae, through the coordinated use of a first fixed seat, a second fixed seat, a fixed sleeve, a first sensor, and a second sensor, allows the heating tube to reciprocate up and down under the drive of a motor while simultaneously heating the evaporation tube, further improving the uniform heating effect of the liquid material inside the evaporation tube. Simultaneously, through the coordinated use of a transfer cylinder, a dialysis device, a second water pump, a third water pipe, and a fourth water pipe, the equipment can perform circulating dialysis of the upper phase liquid inside the transfer cylinder, improving the purity of the extraction.

[0004] During use, it was found that existing concentration and purification equipment for extracting proteins from microalgae cannot ensure sufficient mixing of microalgae and extract during reflux extraction, resulting in low extraction efficiency. Therefore, there is an urgent need for a concentration and purification equipment for extracting proteins from microalgae. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a concentration and purification device for extracting proteins from microalgae, which involves reflux extraction using a reflux extraction device, heating and evaporating the mixture inside the reflux extraction device using an evaporation device, stirring the mixture inside the reflux extraction device using a stirring device, temporarily storing the mixed solution inside the reflux extraction device using an extraction device, extracting the solution inside the extraction device using an extraction device, dialyzing the mixture using a dialysis device, and freeze-drying the mixture using a freeze-drying device.

[0006] This invention relates to a concentration and purification device for extracting proteins from microalgae, comprising an operating table; and further comprising a reflux extraction device, an evaporation device, a stirring device, an extraction device, a desorption device, a dialysis device, and a freeze-drying device. The reflux extraction device, evaporation device, extraction device, desorption device, dialysis device, and freeze-drying device are all mounted on the operating table, and the stirring device is mounted on the reflux extraction device. The reflux extraction device performs reflux extraction, the evaporation device performs heating and evaporation, the stirring device performs stirring, the extraction device stores the solution, the desorption device performs desorption, the dialysis device performs dialysis, and the freeze-drying device performs freeze-drying. The process involves reflux extraction via the reflux extraction device, heating and evaporating the mixture inside the reflux extraction device via the evaporation device, stirring the mixture inside the reflux extraction device via the stirring device, temporarily storing the mixed solution inside the reflux extraction device via the extraction device, desorption of the solution inside the extraction device via the desorption device, dialysis of the mixture via the dialysis device, and freeze-drying of the mixture via the freeze-drying device.

[0007] Preferably, the operating table includes a base, which is installed in the work area; the base provides support.

[0008] Preferably, the reflux extraction device includes an evaporation tube, an input tube, and a first pipe. The evaporation tube is mounted on a base, the input tube is connected to the side end of the evaporation tube, a first valve is provided on the evaporation tube, and the first pipe is connected to the bottom end of the evaporation tube. Microalgae and a 50-70% alcohol-water solution are conveniently placed into the evaporation tube through the input tube, and the first valve is opened to discharge the mixed solution through the output end of the first pipe.

[0009] Preferably, the evaporation device includes a motor, a lead screw, a lifting plate, a mounting bracket, a guide shaft, a guide sleeve, and an electric heating wire. The motor is mounted at the bottom of the base, and its output end is rotatably connected to the input end of the lead screw. The lifting plate is threaded onto the lead screw. The mounting bracket and the guide shaft are both mounted at the top of the base. The guide sleeve is mounted on the lifting plate and fitted onto the guide shaft. The electric heating wire is mounted on the lifting plate and fitted onto the evaporation tube. By starting the motor, the lead screw is driven to rotate, thereby enabling the lifting plate to reciprocate up and down on the guide shaft via the guide sleeve. The electric heating wire evaporates the mixture inside the evaporation tube.

[0010] Preferably, the stirring device includes a second motor and a rotating shaft. The second motor is installed at the top of the evaporation tube, and the output end of the second motor is rotatably connected to the input end of the rotating shaft. Multiple sets of stirring blades are provided on the rotating shaft. When the second motor is started, the rotating shaft and stirring blades are driven to rotate, thereby stirring the solution inside the evaporation tube.

[0011] Preferably, the extraction device includes a temporary storage tank and a feed pipe. The temporary storage tank is installed at the bottom of the base, and the feed pipe is connected to the top of the temporary storage tank. The output end of the first pipe extends into the interior of the temporary storage tank. The crude liquid flows into the interior of the temporary storage tank through the first pipe, and then the modified glucose-inorganic salt aqueous two-phase is added to the interior of the temporary storage tank for extraction and stratification until the crude liquid completes stratification.

[0012] Preferably, the extraction device includes a power pump, a fixed pipe, a lifting pipe, and a second pipe. The power pump is installed at the top of the base. The input end of the power pump is connected to the output end of the fixed pipe, the output end of the lifting pipe is connected to the input end of the fixed pipe, and the output end of the power pump is connected to the input end of the second pipe. By raising and lowering the lifting pipe on the fixed pipe, the bottom end of the lifting pipe is moved to the upper position, the power pump is started, and the upper phase flows out through the output end of the second pipe through the fixed pipe and the lifting pipe.

[0013] Preferably, the dialysis device includes an adapter tube, a third pipe, a second power pump, a fourth pipe, a dialysis machine, and a fifth pipe. The adapter tube is mounted on a base. The output end of the second pipe is connected to the input end of the adapter tube, the input end of the third pipe is connected to the output end of the adapter tube, and the output end of the third pipe is connected to the input end of the second power pump. The second power pump is mounted at the bottom of the base, and its output end is connected to the input end of the fourth pipe. The output end of the fourth pipe is connected to the input end of the dialysis machine, the output end of the dialysis machine is connected to the input end of the fifth pipe, and the output end of the fifth pipe is connected to the input end of the adapter tube. By starting the second power pump, the solution inside the adapter tube enters through the input end of the third pipe and flows into the dialysis machine through the output end of the fourth pipe. The solution enters the dialysis machine for dialysis, and then flows back to the adapter tube through the fifth pipe, achieving cyclic dialysis.

[0014] Preferably, the freeze-drying apparatus includes a sixth pipe and a freeze dryer. The output end of the dialysis machine is connected to the input end of the sixth pipe. A second valve is installed on the sixth pipe. The freeze dryer is installed at the bottom of the base. The output end of the sixth pipe is connected to the input end of the freeze dryer. After dialysis is completed and a puerarin aqueous solution is obtained, the second valve is opened, and the puerarin aqueous solution flows into the interior of the freeze dryer through the sixth pipe for cooling and drying.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows: reflux extraction is performed by a reflux extraction device, the mixture inside the reflux extraction device is heated and evaporated by an evaporation device, the mixture inside the reflux extraction device is stirred by a stirring device, the mixed solution inside the reflux extraction device is temporarily stored by an extraction device, the solution inside the extraction device is extracted by an extraction device, the mixture is dialyzed by a dialysis device, and the mixture is freeze-dried by a freeze-drying device. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 yes Figure 1 A schematic diagram of the structure of the operating table and reflux extraction device in this utility model;

[0018] Figure 3 yes Figure 1 A structural cross-sectional view of the operating table and reflux extraction device in this utility model;

[0019] Figure 4 yes Figure 1 Enlarged structural isometric view of the extraction device and dialysis device in this utility model;

[0020] Figure 5 yes Figure 1 An enlarged side view of the dialysis device and cooling drying device in this utility model.

[0021] The attached diagram is labeled as follows: 01, operating table; 11, base; 02, reflux extraction device; 21, evaporation tube; 22, input tube; 23, first valve; 24, first pipeline; 03, evaporation device; 31, motor one; 32, lead screw; 33, lifting plate; 34, mounting bracket; 35, guide shaft; 36, guide sleeve; 37, electric heating wire; 04, stirring device; 41, motor two; 42, rotating shaft; 43, stirring blade; 05, extraction device; 51, temporary storage box; 52, feed pipe; 06, extraction device; 61, power pump one; 62, fixed pipe; 63, lifting pipe; 64, second pipeline; 07, dialysis device; 71, adapter cylinder; 72, third pipeline; 73, power pump two; 74, fourth pipeline; 75, dialysis machine; 76, fifth pipeline; 08, freeze-drying device; 81, sixth pipeline; 82, second valve; 83, freeze dryer. Detailed Implementation

[0022] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. This utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of this utility model more thorough and complete.

[0023] Example 1

[0024] A concentration and purification device for extracting proteins from microalgae includes an operating table 01; characterized in that it further includes a reflux extraction device 02, an evaporation device 03, a stirring device 04, an extraction device 05, an extraction device 06, a dialysis device 07, and a freeze-drying device 08, wherein the reflux extraction device 02, evaporation device 03, extraction device 05, extraction device 06, dialysis device 07, and freeze-drying device 08 are all installed on the operating table 01, and the stirring device 04 is installed on the reflux extraction device 02;

[0025] Reflux extraction device 02 performs reflux extraction, evaporation device 03 performs heating and evaporation, stirring device 04 performs stirring, extraction device 05 performs storage, extraction device 06 performs extraction, dialysis device 07 performs dialysis, and freeze-drying device 08 performs freeze-drying.

[0026] The operating console 01 includes a base 11, which is installed in the work area;

[0027] The reflux extraction device 02 includes an evaporation tube 21, an input tube 22, and a first pipe 24. The evaporation tube 21 is mounted on the base 11, the input tube 22 is connected to the side end of the evaporation tube 21, a first valve 23 is provided on the evaporation tube 21, and the first pipe 24 is connected to the bottom end of the evaporation tube 21.

[0028] The evaporation device 03 includes a motor 31, a lead screw 32, a lifting plate 33, a mounting bracket 34, a guide shaft 35, a guide sleeve 36, and an electric heating wire 37. The motor 31 is installed at the bottom of the base 11, and the output end of the motor 31 is rotatably connected to the input end of the lead screw 32. The lifting plate 33 is installed on the lead screw 32 by a threaded connection. The mounting bracket 34 and the guide shaft 35 are both installed at the top of the base 11. The guide sleeve 36 is installed on the lifting plate 33 and is fitted onto the guide shaft 35. The electric heating wire 37 is installed on the lifting plate 33 and is fitted onto the evaporation tube 21.

[0029] The extraction device 05 includes a temporary storage box 51 and a feed pipe 52. The temporary storage box 51 is installed at the bottom of the base 11, and the feed pipe 52 is connected to the top of the temporary storage box 51. The output end of the first pipe 24 extends into the interior of the temporary storage box 51.

[0030] The extraction device 06 includes a power pump 61, a fixed pipe 62, a lifting pipe 63, and a second pipe 64. The power pump 61 is installed on the top of the base 11. The input end of the power pump 61 is connected to the output end of the fixed pipe 62. The output end of the lifting pipe 63 is connected to the input end of the fixed pipe 62. The output end of the power pump 61 is connected to the input end of the second pipe 64.

[0031] The dialysis device 07 includes an adapter tube 71, a third pipe 72, a second power pump 73, a fourth pipe 74, a dialysis machine 75, and a fifth pipe 76. The adapter tube 71 is mounted on the base 11. The output end of the second pipe 64 is connected to the input end of the adapter tube 71. The input end of the third pipe 72 is connected to the output end of the adapter tube 71. The output end of the third pipe 72 is connected to the input end of the second power pump 73. The second power pump 73 is mounted on the bottom of the base 11. The output end of the second power pump 73 is connected to the input end of the fourth pipe 74. The output end of the fourth pipe 74 is connected to the input end of the dialysis machine 75. The output end of the dialysis machine 75 is connected to the input end of the fifth pipe 76. The output end of the fifth pipe 76 is connected to the input end of the adapter tube 71.

[0032] The freeze-drying apparatus 08 includes a sixth pipe 81 and a freeze dryer 83. The output end of the dialysis machine 75 is connected to the input end of the sixth pipe 81. A second valve 82 is provided on the sixth pipe 81. The freeze dryer 83 is installed at the bottom end of the base 11. The output end of the sixth pipe 81 is connected to the input end of the freeze dryer 83.

[0033] The reflux extraction device 02 performs reflux extraction, the evaporation device 03 performs heating evaporation, the extraction device 05 performs storage, the extraction device 06 performs extraction, the dialysis device 07 performs dialysis, and the freeze-drying device 08 performs freeze-drying.

[0034] Microalgae and a 50-70% alcohol-water solution are conveniently placed into the evaporation tube 21 through the input pipe 22. The first valve 23 is opened, and the mixed solution is discharged through the output end of the first pipe 24. Then, the motor 31 is started, driving the lead screw 32 to rotate, causing the lifting plate 33 to reciprocate on the guide shaft 35 via the guide sleeve 36. The mixture inside the evaporation tube 21 is evaporated by the electric heating wire 37. Then, the first valve 23 is opened, and the crude liquid flows into the temporary storage tank 51 through the first pipe 24. Modified glucose-inorganic salt aqueous two-phase solution is then added to the temporary storage tank 51 for extraction and layering. After the crude liquid has completed layering, the lifting pipe 63 is used to raise the liquid from the fixed pipe 62. Lower the bottom of the lifting pipe 63 to the top, start the power pump 61, and the upper phase flows out through the output end of the second pipe 64 to the inside of the transfer cylinder 71 through the fixed pipe 62 and the lifting pipe 63. With the start of the second power pump 73, the solution inside the transfer cylinder 71 enters through the input end of the third pipe 72 and flows into the inside of the dialysis machine 75 through the output end of the fourth pipe 74. The solution enters the dialysis machine 75 for dialysis. The solution flows back to the transfer cylinder 71 through the fifth pipe 76 to achieve cyclic dialysis. After dialysis is completed, a puerarin aqueous solution is obtained. Open the second valve 82, and the puerarin aqueous solution flows into the inside of the freeze dryer 83 through the sixth pipe 81 for cooling and drying.

[0035] Example 2

[0036] like Figures 1 to 5 As shown, in addition to Embodiment 1, a stirring device 04 is also included;

[0037] The stirring device 04 includes a second motor 41 and a rotating shaft 42. The second motor 41 is installed at the top of the evaporation tube 21. The output end of the second motor 41 is rotatably connected to the input end of the rotating shaft 42. Multiple sets of stirring blades 43 are provided on the rotating shaft 42.

[0038] Stirring device 04 is used for stirring;

[0039] When the motor 41 is started, the rotating shaft 42 and the stirring blade 43 are driven to rotate, stirring the solution inside the evaporation tube 21.

[0040] This invention relates to a concentration and purification device for extracting proteins from microalgae. During operation, microalgae and a 50-70% alcohol-water solution are first conveniently placed into the evaporation tube 21 via the input pipe 22. The first valve 23 is opened, and the mixed solution is discharged through the output end of the first pipe 24. Then, the second motor 41 is started, driving the rotating shaft 42 and stirring blades 43 to rotate, stirring the solution inside the evaporation tube 21. Simultaneously, the first motor 31 is started, driving the lead screw 32 to rotate, causing the lifting plate 33 to reciprocate on the guide shaft 35 via the guide sleeve 36. The mixture inside the evaporation tube 21 is evaporated by the electric heating wire 37. Then, the first valve 23 is opened, and the crude liquid flows into the temporary storage tank 51 through the first pipe 24. Finally, a modified glucose-inorganic salt aqueous two-phase solution is added to the temporary storage tank 51. Extraction and stratification are performed inside the dialysis unit 1. After the crude liquid has completed stratification, the bottom end of the lifting tube 63 is moved to the upper phase by raising and lowering the lifting tube 63 above the fixed tube 62. The power pump 61 is started, and the upper phase flows out through the output end of the second pipe 64 to the inside of the transfer cylinder 71 through the fixed tube 62 and the lifting tube 63. The solution inside the transfer cylinder 71 is then introduced through the input end of the third pipe 72 and flows into the inside of the dialysis machine 75 through the output end of the fourth pipe 74. The solution enters the dialysis machine 75 for dialysis and then flows back to the transfer cylinder 71 through the fifth pipe 76 to achieve cyclic dialysis. After dialysis is completed, a puerarin aqueous solution is obtained. The second valve 82 is opened, and the puerarin aqueous solution flows into the freeze dryer 83 through the sixth pipe 81 for cooling and drying.

[0041] The motor 31, motor 41, power pump 61, power pump 73, and freeze dryer 83 of this utility model are commercially available. Technical personnel in this industry only need to install and operate them according to the accompanying instruction manual, without requiring any creative work from those skilled in the art.

[0042] The main function of this invention is to stir the solution in the reflux extraction device, thereby improving efficiency.

[0043] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A concentration and purification device for extracting proteins from microalgae, comprising an operating table (01); characterized in that, It also includes a reflux extraction device (02), an evaporation device (03), a stirring device (04), an extraction device (05), an extraction device (06), a dialysis device (07), and a freeze-drying device (08). The reflux extraction device (02), evaporation device (03), extraction device (05), extraction device (06), dialysis device (07), and freeze-drying device (08) are all installed on the operating table (01), and the stirring device (04) is installed on the reflux extraction device (02). The reflux extraction device (02) performs reflux extraction, the evaporation device (03) performs heating evaporation, the stirring device (04) performs stirring, the extraction device (05) performs storage, the extraction device (06) performs extraction, the dialysis device (07) performs dialysis, and the freeze-drying device (08) performs freeze-drying.

2. The concentration and purification equipment for extracting proteins from microalgae as described in claim 1, characterized in that, The operating console (01) includes a base (11), which is installed in the work area.

3. The concentration and purification equipment for extracting proteins from microalgae as described in claim 2, characterized in that, The reflux extraction device (02) includes an evaporation tube (21), an input tube (22), and a first pipe (24). The evaporation tube (21) is mounted on the base (11), the input tube (22) is connected to the side end of the evaporation tube (21), a first valve (23) is provided on the evaporation tube (21), and the first pipe (24) is connected to the bottom end of the evaporation tube (21).

4. The concentration and purification equipment for extracting proteins from microalgae as described in claim 3, characterized in that, The evaporation device (03) includes a motor (31), a lead screw (32), a lifting plate (33), a mounting bracket (34), a guide shaft (35), a guide sleeve (36), and an electric heating wire (37). The motor (31) is installed at the bottom of the base (11), and the output end of the motor (31) is rotatably connected to the input end of the lead screw (32). The lifting plate (33) is installed on the lead screw (32) by a threaded connection. The mounting bracket (34) and the guide shaft (35) are both installed at the top of the base (11). The guide sleeve (36) is installed on the lifting plate (33) and is fitted onto the guide shaft (35). The electric heating wire (37) is installed on the lifting plate (33) and is fitted onto the evaporation tube (21).

5. The concentration and purification equipment for extracting proteins from microalgae as described in claim 3, characterized in that, The stirring device (04) includes a second motor (41) and a rotating shaft (42). The second motor (41) is installed at the top of the evaporation tube (21). The output end of the second motor (41) is rotatably connected to the input end of the rotating shaft (42). Multiple sets of stirring blades (43) are provided on the rotating shaft (42).

6. The concentration and purification equipment for extracting proteins from microalgae as described in claim 3, characterized in that, The extraction device (05) includes a temporary storage box (51) and a feed pipe (52). The temporary storage box (51) is installed at the bottom of the base (11), and the feed pipe (52) is connected to the top of the temporary storage box (51). The output end of the first pipe (24) extends into the interior of the temporary storage box (51).

7. The concentration and purification equipment for extracting proteins from microalgae as described in claim 2, characterized in that, The extraction device (06) includes a power pump (61), a fixed pipe (62), a lifting pipe (63), and a second pipe (64). The power pump (61) is installed at the top of the base (11). The input end of the power pump (61) is connected to the output end of the fixed pipe (62). The output end of the lifting pipe (63) is connected to the input end of the fixed pipe (62). The output end of the power pump (61) is connected to the input end of the second pipe (64).

8. The concentration and purification equipment for extracting proteins from microalgae as described in claim 7, characterized in that, The dialysis device (07) includes an adapter tube (71), a third pipe (72), a second power pump (73), a fourth pipe (74), a dialysis machine (75), and a fifth pipe (76). The adapter tube (71) is mounted on the base (11). The output end of the second pipe (64) is connected to the input end of the adapter tube (71). The input end of the third pipe (72) is connected to the output end of the adapter tube (71). The output end of the third pipe (72) is connected to the input end of the second power pump (73). The second power pump (73) is mounted on the bottom of the base (11). The output end of the second power pump (73) is connected to the input end of the fourth pipe (74). The output end of the fourth pipe (74) is connected to the input end of the dialysis machine (75). The output end of the dialysis machine (75) is connected to the input end of the fifth pipe (76). The output end of the fifth pipe (76) is connected to the input end of the adapter tube (71).

9. The concentration and purification equipment for extracting proteins from microalgae as described in claim 8, characterized in that, The freeze-drying apparatus (08) includes a sixth pipe (81) and a freeze dryer (83). The output end of the dialysis machine (75) is connected to the input end of the sixth pipe (81). A second valve (82) is provided on the sixth pipe (81). The freeze dryer (83) is installed at the bottom end of the base (11). The output end of the sixth pipe (81) is connected to the input end of the freeze dryer (83).

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