Sampling device suitable for bioreactor

Abstract

The utility model discloses a sampling device suitable for a bioreactor, which comprises the bioreactor, the top of the bioreactor is connected with a top cover, the top cover is respectively connected with an exhaust pipe and a sampling pipe extending into the bioreactor, and a return pipe is connected between the exhaust pipe and the sampling pipe; the top cover is connected with a pipe diameter control assembly around the exhaust pipe, and the end part of the sampling pipe is also connected with a gas supply pipe. Compared with the prior art, the sampling device suitable for the bioreactor has the advantages that a peristaltic pump is not needed, the waste of a culture solution is reduced, and the residue of a pipe body is reduced.

Description

Technical Field

[0001] This utility model relates to the field of sampling equipment technology, specifically to a sampling device suitable for bioreactors. Background Technology

[0002] A bioreactor is a reaction system that utilizes naturally occurring microorganisms or microorganisms with special degradation capabilities to inoculate the liquid or solid phase.

[0003] In microbial research, bioreactors are often used for cell culture. During cell culture, aseptic sampling of the bioreactor is required for detection.

[0004] Currently, aseptic sampling is performed using a sampling tube (usually a silicone tube) in conjunction with a peristaltic pump to extract the culture medium.

[0005] During operation, residual liquid often remains in the sampling tube, which can lead to waste of culture medium. Utility Model Content

[0006] (I) Problems to be solved

[0007] The technical problem to be solved by this utility model is to overcome the above-mentioned technical defects and provide a sampling device suitable for bioreactors that does not require the use of a peristaltic pump, reduces culture medium waste, and reduces tube residue.

[0008] (II) Technical Solution

[0009] To solve the above-mentioned technical problems, the technical solution provided by this utility model is as follows: a sampling device suitable for a bioreactor, including a bioreactor, a top cover connected to the top of the bioreactor, an exhaust pipe and a sampling pipe extending into the bioreactor respectively connected to the top cover, and a return pipe connected between the exhaust pipe and the sampling pipe.

[0010] The top cover is provided with a pipe diameter control component connected around the exhaust pipe, and the end of the sampling pipe is also connected with an air supply pipe.

[0011] As an improvement, a three-way valve one and a three-way valve two are respectively sleeved on the sampling tube, the end of the return tube is connected to the three-way valve one, and the end of the gas supply tube is connected to the three-way valve two.

[0012] As an improvement, the pipe diameter control assembly includes a support leg connected to the top of the top cover and a collar fixed to the top of the support leg, the collar being sleeved on the outside of the exhaust pipe.

[0013] The collar also has recessed threaded grooves on both sides, and an adjusting screw is rotatably connected in the threaded grooves. A pressure stop block is connected to the end of the adjusting screw near the exhaust pipe.

[0014] As an improvement, control valve one and control valve two are respectively connected to the return pipe and the air supply pipe.

[0015] As an improvement, the sampling tube, exhaust tube, and return tube are all made of flexible tubing.

[0016] (III) Beneficial Effects

[0017] The advantages of this utility model compared with the prior art are as follows: When used in this application, the structural design of the exhaust pipe, sampling pipe, and return pipe, combined with control valve one, control valve two, and pipe diameter control components, can control the pressure inside the reactor by changing the exhaust pipe diameter of the bioreactor, thereby sampling in the form of pressure overflow. After sampling, the sampling pipe can be backflushed by the gas during exhaust, thereby reducing the residue of the sampling liquid in the sampling pipe. In this application, the additional gas supply pipe can also increase the tank pressure by injecting gas from the outside and then sampling through the sampling pipe, thereby increasing the sampling efficiency. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of a sampling device suitable for bioreactors.

[0019] Figure 2 This is a second-view structural schematic diagram of a sampling device suitable for bioreactors.

[0020] Figure 3 This is a structural schematic diagram of the cross-section of the pipe diameter control component.

[0021] As shown in the figure: 1. Bioreactor; 2. Top cover; 3. Exhaust pipe; 4. Sampling pipe; 5. Return pipe; 6. Gas supply pipe; 7. Three-way valve one; 8. Three-way valve two; 9. Support leg; 10. Collar; 11. Adjusting screw; 12. Pressure stop block; 13. Control valve one; 14. Control valve two. Detailed Implementation

[0022] The specific embodiments of this utility model will be further described below with reference to the accompanying drawings. Identical components are indicated by the same reference numerals.

[0023] It should be noted that the terms “front,” “back,” “left,” “right,” “up,” and “down” used in the following description refer to the directions shown in the attached diagram, while the terms “inside” and “outside” refer to the directions toward or away from the geometric center of a specific component, respectively.

[0024] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly associated with those skilled in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments and is not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0026] To make the content of this utility model easier to understand, the technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings.

[0027] Please refer to the appendix carefully. Figure 1-3 A sampling device suitable for a bioreactor includes a bioreactor 1. A top cover 2 is connected to the top of the bioreactor 1. An exhaust pipe 3 and a sampling pipe 4 extending into the bioreactor 1 are respectively connected to the top cover 2. A return pipe 5 is connected between the exhaust pipe 3 and the sampling pipe 4. A pipe diameter control component is connected around the exhaust pipe 3 on the top cover 2. An air supply pipe 6 is also connected to the end of the sampling pipe 4. In order to ensure the control of the connection state of each pipe, a control valve 13 and a control valve 2 14 are respectively connected to the return pipe 5 and the air supply pipe 6.

[0028] Three-way valve 7 and three-way valve 8 are respectively sleeved on the sampling tube 4. The end of the return pipe 5 is connected to three-way valve 7, and the end of the air supply pipe 6 is connected to three-way valve 8, which facilitates subsequent disassembly, assembly and maintenance.

[0029] To control the inner diameter of the exhaust pipe 3 and thus the pressure inside the reactor, the pipe diameter control assembly includes a support leg 9 connected to the top of the top cover 2 and a collar 10 fixed to the top of the support leg 9. The collar 10 is sleeved on the outside of the exhaust pipe 3. More specifically, the collar 10 has recessed threaded grooves on both sides, and an adjusting screw 11 is rotatably connected in the threaded grooves. A pressure stop block 12 is connected to the end of the adjusting screw 11 near the exhaust pipe 3. The inner diameter of the exhaust pipe 3 is controlled to decrease by pressing the exhaust pipe 3 with the pressure stop block 12.

[0030] In practical applications, the sampling tube 4, the exhaust tube 3, and the return tube 5 are all made of flexible tubing.

[0031] In a specific implementation of this invention, control valve one and control valve two are in the closed state. At this time, the inner diameter of the exhaust pipe is reduced by turning the adjusting screw. When the inner diameter of the exhaust pipe is reduced, the pressure inside the reactor is in the rising state. At this time, the sampling liquid is discharged through the sampling tube. After sampling, control valve one is opened, and part of the gas in the exhaust pipe flows back to the sampling tube through the return pipe. The end of the sampling tube is in the closed state, and the residual liquid flows back to the reactor under the pressure of the gas.

[0032] During another sampling operation, the exhaust pipe is stopped by adjusting the screw to control the pressure stop block. The reactor is then filled with gas through the gas supply pipe by opening control valve two. After filling, control valve two is closed. Due to the high pressure inside the reactor, the sample liquid flows back to the outside through the sampling tube for sampling. After sampling, the control screw is opened to release the gas, and control valve one is opened at the same time to allow some gas to flow back to the sampling tube, thereby allowing the residual liquid to flow back to the reactor under gas pressure.

[0033] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0034] All standard parts used in this utility model can be purchased from the market, and irregular parts can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art. In addition, the circuit connection adopts conventional connection methods in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0035] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit of the present invention, such designs should fall within the protection scope of the present invention.

Claims

1. A sampling device suitable for a bioreactor, comprising a bioreactor (1), a top cover (2) connected to the top of the bioreactor (1), characterized in that: an exhaust pipe (3) and a sampling pipe (4) extending into the bioreactor (1) are connected to the top cover (2) respectively, a backflow pipe (5) is connected between the exhaust pipe (3) and the sampling pipe (4); a pipe diameter control assembly is connected around the exhaust pipe (3) on the top cover (2), and an air supply pipe (6) is further connected to the end of the sampling pipe (4).

2. A sampling device suitable for use with a bioreactor according to claim 1, wherein: A three-way valve I (7) and a three-way valve II (8) are respectively sleeved on the sampling pipe (4), the end of the backflow pipe (5) is connected to the three-way valve I (7), and the connecting end of the air supply pipe (6) is connected to the three-way valve II (8).

3. A sampling device suitable for use with a bioreactor according to claim 1, wherein: The pipe diameter control assembly comprises a leg (9) connected to the top of the top cover (2) and a sleeve ring (10) fixed to the top of the leg (9), and the sleeve ring (10) is sleeved outside the exhaust pipe (3); Both sides of the sleeve ring (10) are further recessed with screw grooves, and an adjusting screw (11) is rotatably connected in the screw grooves, and the end of the adjusting screw (11) is connected with a pressing block (12) near one side of the exhaust pipe (3).

4. A sampling device for a bioreactor according to any one of claims 1 to 3, wherein: Control valve I (13) and control valve II (14) are respectively connected to the backflow pipe (5) and the air supply pipe (6).

5. A sampling device suitable for use with a bioreactor according to claim 4, wherein: The sampling pipe (4), the exhaust pipe (3) and the backflow pipe (5) are all made of soft pipe material.

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