Gas distribution culture system capable of being freely combined

Abstract

The utility model relates to a gas distribution culture system capable of being freely combined. Comprising a plurality of gas supply single paths for supplying different gases, a multi-way joint for communicating the plurality of gas supply single paths, a gas mixing tank communicated with the multi-way joint, a back pressure type pressure controller I communicated with the gas mixing tank, a culture device communicated with the back pressure type pressure controller I through a gas inlet end, and a back pressure type pressure controller II communicated with a gas outlet end of the culture device, the gas supply single path comprises a gas cylinder, a mass flow controller and a one-way valve which are sequentially arranged, the one-way valve is communicated with the multi-way connector through a pipeline, and a filter is connected to the pipeline between the gas cylinder and the mass flow controller. The gas distribution culture system is simple in gas circuit structure, the internal pressure of the gas distribution system can be automatically adjusted in a segmented mode, and the pressure of different stages can be adjusted according to using conditions.

Description

Technical Field

[0001] This application relates to the technical field of equipment related to in vitro embryo culture, and in particular to a freely combinable gas-mixing culture system. Background Technology

[0002] In vitro embryo culture involves placing a culture dish containing fertilized eggs or embryos in a conventional carbon dioxide incubator, tri-gas incubator, or time-zone incubator. Embryo culture requires a highly controlled surrounding air environment. To ensure normal embryo development within the incubator, current techniques involve pre-mixing air, pure carbon dioxide, and pure nitrogen in a specific ratio to create a premixed gas. This premixed gas, at a specific concentration, is then introduced into the embryo culture chamber to meet the requirements of embryo culture.

[0003] The supply of premixed gas requires adjustment and control of the gas circuit. However, the existing gas supply circuit requires manual control of the pressure reducing valve to adjust the gas distribution pressure, which has poor accuracy and stability. In addition, the gas circuit has a complex structure and many parts. Utility Model Content

[0004] The purpose of this application is to provide a freely combinable gas mixing and culture system with a simple gas path structure. The internal pressure of the gas mixing system can be automatically adjusted in segments, and the pressure at different stages can be adjusted according to the usage conditions.

[0005] The freely combinable gas mixing culture system provided in this application adopts the following technical solution:

[0006] A freely combinable gas mixing and culture system includes several gas supply lines supplying different gases, a multi-port connector connecting the several gas supply lines, a mixing tank connected to the multi-port connector, a back pressure type pressure control I connected to the mixing tank, a culture device connected to the back pressure type pressure control I through the gas inlet end, and a back pressure type pressure control II connected to the gas outlet end of the culture device.

[0007] As a preferred technical solution of this application, the gas supply single circuit includes a gas cylinder, a mass flow controller and a one-way valve arranged in sequence, and the one-way valve is connected to a multi-port connector through a pipeline.

[0008] As a preferred technical solution of this application, a filter is connected to the pipeline between the gas cylinder and the mass flow controller.

[0009] As a preferred technical solution of this application, the mass flow controller includes a solenoid valve, a flow measurement unit, and a control system. The solenoid valve and the flow measurement unit are connected in series in the pipeline between the gas cylinder and the check valve, and the control system is connected to the solenoid valve and the flow measurement unit respectively.

[0010] As a preferred technical solution of this application, the outlet end of the back pressure type pressure control device is provided with an observation bottle.

[0011] As a preferred technical solution of this application, the back pressure type one and the back pressure type two have the same structure, including a back pressure valve, a back pressure sensor and a control system, and the control system is connected to the back pressure valve and the back pressure sensor respectively.

[0012] As a preferred technical solution of this application, when the back pressure control one is set to a certain value, the back pressure sensor of the back pressure control two is set to 0, which can achieve constant gas pressure in the mixing tank and stably supply gas to the culture device.

[0013] As a preferred technical solution of this application, when the back pressure control device II is set to a certain value, the back pressure sensor of the back pressure control device I is set to 0, which can achieve constant gas pressure in the culture device and facilitate gas exchange during embryo culture.

[0014] In summary, this application includes at least one of the following beneficial technical effects:

[0015] 1. The internal pressure of the gas distribution system in this application can be adjusted in segments, making it more convenient to use.

[0016] 2. The gas distribution system of this application has a simple structure and can adjust the pressure at different stages according to conditions.

[0017] 3. One instrument in this system can achieve both positive and back pressure test states of the culture device, eliminating the need for two gas mixing systems. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the cultivation system according to an embodiment of this application;

[0019] In the diagram, 1. Gas cylinder; 10. Observation bottle; 11. Control system; 2. Filter; 3. Mass flow controller; 31. Solenoid valve; 32. Flow measurement unit; 4. Check valve; 5. Multi-port connector; 6. Mixing tank; 7. Back pressure type pressure control one; 71. Back pressure valve; 72. Back pressure sensor; 8. Culture device; 9. Back pressure type pressure control two. Detailed Implementation

[0020] The present application will be further described in detail below with reference to the accompanying drawings.

[0021] Example: This example proposes a freely combinable gas mixing culture system, referring to... Figure 1The system includes a single gas supply line, a multi-port connector 5, a gas mixing tank 6, a back pressure type pressure control 7, and a culture device 8. Several single gas supply lines are connected in parallel to supply different pure or premixed gases. The multi-port connector 5 connects multiple single gas supply lines to mix the various gases together and then enters the gas mixing tank 6 for mixing. The back pressure type pressure control 7 is connected to the gas outlet of the gas mixing tank 6. The culture device 8 is connected to the back pressure type pressure control 7, which controls the pressure value entering the culture device 8.

[0022] In this embodiment, the gas supply line is set with three sets, which are used to supply pure oxygen, pure carbon dioxide and pure nitrogen respectively.

[0023] Each gas supply line includes a gas cylinder 1, a filter 2, a mass flow controller 3, and a one-way valve 4, arranged sequentially. Gas cylinder 1 is located at the very end, and a valve is installed on the pipe outside the cylinder opening to control its opening and closing. Gas cylinder 1 is connected to filter 2 via a pipe; filter 2 filters the gas to remove dust. Filter 2 is connected to mass flow controller 3 via a pipe; mass flow controller 3 controls the gas flow rate. Mass flow controller 3 is connected to one-way valve 4 via a pipe; one-way valve 4 prevents gas backflow from affecting the gas supply ratio. One-way valve 4 is connected to a multi-way connector 5 via a pipe. In this embodiment, multi-way connector 5 is a four-way connector; three connectors connect to three gas supply lines, and the other connector connects to a mixing tank 6 via a pipe.

[0024] The mixing tank 6 is used for gas mixing. By setting the value of the mass flow controller 3 corresponding to each gas, the total amount and proportion of the three gases can be calculated. The mixing tank 6 is connected to the back pressure control 7 through a pipeline. The back pressure control 7 is connected to the culture device 8 through a pipeline. After the mixed gas passes through the back pressure control 7, it is delivered to the culture device 8 for embryo growth.

[0025] In this embodiment, the gas output end of the culture device 8 is connected to a back pressure type pressure controller 9 via a pipeline. The back pressure type pressure controller 9 is used to control the gas pressure output from the culture device 8. The back pressure type pressure controller 9 is connected to an observation bottle 10 via a pipeline, through which the discharged gas can be observed.

[0026] In this embodiment, a back pressure type pressure control 7 and a back pressure type pressure control 9 are respectively set on the air inlet side and air outlet side of the culture device 8 to achieve precise control of the pressure state in the culture device 8.

[0027] The mass flow controller 3 includes a solenoid valve 31, a flow measurement unit 32, and a control system 11. The solenoid valve 31 and the flow measurement unit 32 are connected in series in the pipeline between the filter 2 and the check valve 4. The flow measurement unit 32 is a flow meter. The control system 11 is connected to the solenoid valve 31 and the flow measurement unit 32 respectively. The control system 11 includes a controller part and a display screen part, which are used to control the operation of the entire system and display data of various aspects of the system respectively.

[0028] The back pressure type pressure control system 7 includes a back pressure valve 71, a back pressure sensor 72, and a control system 11. The back pressure valve 71 is connected to the main pipeline, and the back pressure sensor 72 is connected to the pipeline at the inlet end of the back pressure valve 71. The control system 11 is connected to both the back pressure valve 71 and the back pressure sensor 72. All equipment parameters in this gas circuit system are set through the control system 11, ensuring high precision and accuracy in gas flow and pressure control.

[0029] When back pressure control 7 is set to a certain value, back pressure sensor 72 of back pressure control 9 is set to 0, which can keep the gas pressure in mixing tank 6 constant and stably supply gas to culture device 8. This is equivalent to pre-pressurizing the mixed gas to facilitate gas system circulation; this method is a positive pressure control system.

[0030] When the back pressure control 29 is set to a certain value, the back pressure sensor 72 of the back pressure control 17 is set to 0, which can keep the gas pressure in the culture device 8 constant and increase the gas concentration in the reaction chamber of the culture device 8, facilitating gas exchange during embryo culture. By setting two different back pressure control settings, the exploration of the culture gas environment can be satisfied. This method is a back pressure control system.

[0031] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Identical components are represented by the same reference numerals. Therefore, all equivalent changes made to the structure, shape, and principle of this application should be covered within the scope of protection of this application.

Claims

1. A freely combinable gas distribution culture system, characterized by, The application relates to a gas supply device for embryo culture, which comprises a plurality of gas supply lines, a multi-way joint (5) for connecting the gas supply lines, a gas mixing tank (6) connected with the multi-way joint (5), a back pressure type pressure control I (7) connected with the gas mixing tank (6), a culture device (8) connected with the back pressure type pressure control I (7) through an air inlet end, and a back pressure type pressure control II (9) connected with an air outlet end of the culture device (8).

2. The freely combinable air distribution incubation system according to claim 1, characterized in that, The gas supply line comprises a gas cylinder (1), a mass flow controller (3) and a check valve (4) arranged in sequence, and the check valve (4) is connected with the multi-way joint (5) through a pipeline.

3. The freely combinable air distribution incubation system according to claim 2, characterized in that, A filter (2) is connected to the pipeline between the gas cylinder (1) and the mass flow controller (3).

4. The freely combinable air distribution incubation system according to claim 2, wherein, The mass flow controller (3) comprises an electromagnetic valve (31), a flow measurement unit (32) and a control system (11), the electromagnetic valve (31) and the flow measurement unit (32) are connected in series to the pipeline between the gas cylinder (1) and the check valve (4), and the control system (11) is connected with the electromagnetic valve (31) and the flow measurement unit (32) respectively.

5. The freely combinable air distribution incubation system according to any one of claims 1-4, characterized in that, An observation bottle (10) is arranged at the air outlet end of the back pressure type pressure control II (9).

6. The freely combinable air distribution incubation system according to any one of claims 1-4, characterized in that, The back pressure type pressure control I (7) and the back pressure type pressure control II (9) have the same structure, and each comprises a back pressure valve (71), a back pressure sensor (72) and a control system (11), and the control system (11) is connected with the back pressure valve (71) and the back pressure sensor (72) respectively.

7. The freely combinable air distribution incubation system according to claim 6, characterized in that, When the back pressure type pressure control I (7) is set to a certain value, the back pressure sensor (72) of the back pressure type pressure control II (9) is set to 0, the gas pressure in the gas mixing tank (6) can be kept constant, and the culture device (8) can be provided with stable gas.

8. The freely combinable air distribution incubation system according to claim 6, characterized in that, When the back pressure type pressure control II (9) is set to a certain value, the back pressure sensor (72) of the back pressure type pressure control I (7) is set to 0, the gas pressure in the culture device (8) can be kept constant, and the gas exchange during embryo culture is facilitated.

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