Portable fluorescence detection device

Abstract

The utility model discloses a portable fluorescence detection device, which relates to the technical field of fluorescence detection and comprises a shell, and at least one laser generator, at least one first optical fiber, at least one sample holder and at least one second optical fiber which are arranged in the shell, a placement groove is formed in the upper portion of the shell, a mobile phone is placed in the placement groove, and a detection hole is formed in the bottom wall of the placement groove; the sample rack is used for placing samples; the two ends of each first optical fiber are arranged opposite to the output end of the laser generator and the sample respectively, and laser beams emitted by the laser generator can be transmitted to the sample through the first optical fibers and excite fluorescent substances in the sample to emit fluorescent light; the two ends of each second optical fiber are respectively arranged opposite to the sample and the detection hole, and the fluorescent light can enter a camera of the mobile phone through each second optical fiber and the detection hole; the mobile phone can be used for fluorescence detection of the sample. The device is low in cost, convenient to operate, simple in structure, wide in application range and high in detection accuracy and sensitivity.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence detection technology, and in particular to a portable fluorescence detection device. Background Technology

[0002] Fluorescence detection is a very common method in biochemical analysis. Traditional fluorescence detection devices include specialized equipment such as PCR amplifiers, large-scale excitation devices, and spectrometers. The method for analyzing fluorescence intensity is as follows: 1. After obtaining the sample, amplify the sample in a laboratory environment using an amplification instrument such as a PCR instrument to obtain a higher fluorescence intensity; 2. Excite the sample with fluorescence using a large-scale excitation device; 3. Analyze the fluorescence intensity using specialized equipment such as a spectrometer. Traditional fluorescence detection devices are expensive; they have many operational requirements, limiting their application to the laboratory; they are complex to operate, time-consuming, and inefficient.

[0003] Chinese patent CN110487767B discloses a portable upconversion fluorescence detector, which includes a main instrument body and a replaceable sample module. The main instrument body contains a main control chip, a light source module for providing near-infrared laser to excite upconversion fluorescence, a detector, and a wireless module. The replaceable sample module contains an optical path system, which includes multiple components such as a lens group and a reflector, and the optical path design is relatively complex. This patent uses image recognition technology to detect different types of samples in different types of replaceable sample chamber modules. The sample is excited by the near-infrared light source, the optical path system collects the signal, the detector collects the fluorescence signal excited by the sample and images it, and the main control chip receives the sample fluorescence signal imaging information and performs image recognition and processing to calculate the sample concentration. This patent requires additional configuration of a dedicated detector and main control chip for analysis and detection, which results in high cost and a relatively complex structure. Utility Model Content

[0004] The purpose of this invention is to provide a portable fluorescence detection device to solve the problems existing in the prior art. It is low in cost, easy to operate, simple in structure, can be applied in a variety of scenarios, has a wide range of applications, and has high detection accuracy and sensitivity.

[0005] To achieve the above objectives, this utility model provides the following solution:

[0006] This invention provides a portable fluorescence detection device, comprising a housing and a laser generator, a first optical fiber, a sample holder, and a second optical fiber disposed within the housing, wherein there is at least one first optical fiber and one second optical fiber; a placement slot is provided on the top of the housing for placing a mobile phone, and a detection hole is provided on the bottom wall of the placement slot; the sample holder is used to place a sample; the two ends of each first optical fiber are respectively positioned opposite to the output end of the laser generator and the sample, and the laser beam emitted by the laser generator can be transmitted to the sample through each first optical fiber and excite the fluorescent substance in the sample to emit fluorescence; the two ends of each second optical fiber are respectively positioned opposite to the sample and the detection hole, and the fluorescence can be incident on the camera of the mobile phone through each second optical fiber and the detection hole; the mobile phone can be used to perform fluorescence detection on the sample.

[0007] Preferably, the device further includes an optical fiber fixing device. The sample holder has at least one first optical fiber hole. The end of each first optical fiber near the sample holder is fixedly connected to one of the first optical fiber holes. The end of each second optical fiber near the sample holder is fixedly connected to one of the first optical fiber holes. The optical fiber fixing device is connected to the inner bottom wall of the housing. The optical fiber fixing device has at least one second optical fiber hole. Each second optical fiber hole is disposed opposite to the detection hole. The end of each second optical fiber away from the sample holder is fixedly connected to one of the second optical fiber holes.

[0008] Preferably, the outer casing includes a casing body and a placement plate. The placement plate is provided with the detection hole. The top of the casing body is provided with a mounting hole. At least two grooves are formed on the upper end face of the side wall of the casing body, and each groove communicates with the mounting hole. The placement plate includes a plate body and at least two protrusions. Each protrusion is fixedly connected to the plate body. By overlapping each protrusion with the inner bottom wall of one of the grooves, the plate body can be connected to the mounting hole of the casing body. The portion of the upper end of the side wall of the casing body that protrudes above the plate body can form a placement groove with the plate body. The inner side wall of the placement groove can restrict the horizontal movement of the mobile phone.

[0009] Preferably, it further includes a filter component and a fixing cylinder. The fixing cylinder can be fixedly connected to the lower surface of the placement plate. The fixing cylinder is disposed opposite to the detection hole. The filter component can be fixedly connected inside the fixing cylinder. The fluorescence emitted from each of the second optical fibers can pass through the filter component and enter the camera of the mobile phone.

[0010] Preferably, the sample rack includes a support frame, a test tube rack, and an optical fiber fixing plate. The test tube rack is fixedly connected to one end of the support frame, and the test tube rack is provided with at least one test tube hole. The optical fiber fixing plate is fixedly connected to the other end of the support frame. The side of the support frame near the optical fiber fixing plate is used to contact the inner bottom wall of the outer shell. The optical fiber fixing plate is provided with at least one first optical fiber hole, and each of the test tube holes is opposite to at least one first optical fiber hole.

[0011] Preferably, the support frame includes a support plate and at least one triangular bracket, each of the triangular brackets being fixedly connected to one end of the support plate, and the test tube rack being disposed at the other end of the support plate. The side of the triangular bracket away from the test tube rack and the side of the support plate away from the test tube rack can both contact the inner bottom wall of the outer shell.

[0012] Preferably, a first through hole is provided on one side wall of the outer shell, the outer side wall of the support plate can match the inner bottom wall of the first through hole, and each of the triangular brackets, the test tube rack and the optical fiber fixing plate can pass through the first through hole.

[0013] Preferably, the sample holder also includes a heating device, which is disposed on the sample holder and is capable of heating the sample.

[0014] Preferably, the housing further includes a cover, the laser generator and the sample holder are respectively disposed on both sides inside the housing, and a second through hole is provided on the side wall of the housing body near the laser generator, and the cover is detachably fixedly connected to the second through hole.

[0015] Preferably, the cover includes a first cover layer and a second cover layer connected to each other, the outer edge of the first cover layer protrudes beyond the outer edge of the second cover layer, and the outer side wall of the second cover layer can be interference-fitted with the inner side wall of the second through hole; the outer shell has an extension surface below the inner bottom wall of the second through hole, and when the second cover layer is inserted into the second through hole, the outer bottom wall of the first cover layer can contact the extension surface.

[0016] The present invention achieves the following technical advantages over the prior art:

[0017] This invention provides a portable fluorescence detection device, including a housing and a laser generator, a first optical fiber, a sample holder, and a second optical fiber disposed within the housing. A placement slot is provided on the top of the housing for placing a mobile phone, and a detection hole is provided on the bottom wall of the placement slot. The two ends of each first optical fiber are respectively positioned opposite the output end of the laser generator and the sample. The laser beam emitted by the laser generator can be transmitted through each first optical fiber to the sample and excite the fluorescent material in the sample to emit fluorescence. The two ends of each second optical fiber are respectively positioned opposite the sample and the detection hole, and the fluorescence can be incident on the camera of the mobile phone through each second optical fiber and the detection hole. The mobile phone can be used to perform fluorescence detection on the sample.

[0018] This invention uses a laser emitter to emit a laser beam, which is transmitted through a first optical fiber and directed onto a sample, exciting the fluorescent material in the sample to produce fluorescence. The fluorescence is then transmitted through a second optical fiber to a detection aperture, where a specific light spot is formed at the end of the second optical fiber. The beam emitted from this spot passes through the second optical fibers and the detection aperture and is then incident on the camera of a mobile phone, enabling fluorescence detection via the phone. This invention completes fluorescence detection using only a laser emitter, a first optical fiber, a second optical fiber, and a mobile phone, eliminating the need for specialized equipment such as PCR amplifiers and spectrometers. It is low-cost, easy to operate, and has a simple structure, making it applicable in various scenarios and with a wide range of applications. The use of the first and second optical fibers for beam transmission simplifies the optical path design and effectively avoids the influence of external stray light, ensuring detection accuracy. Furthermore, this invention completes fluorescence detection via a mobile phone, eliminating the need for additional detectors and further simplifying the structure and reducing costs. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 Explosion of the portable fluorescence detection device provided by this utility model Figure 1 ;

[0021] Figure 2 Explosion of the portable fluorescence detection device provided by this utility model Figure 2 ;

[0022] Figure 3 Schematic diagram of the portable fluorescence detection device provided by this utility model Figure 1 ;

[0023] Figure 4Schematic diagram of the portable fluorescence detection device provided by this utility model Figure 2 ;

[0024] Figure 5 A cross-sectional view of the portable fluorescence detection device provided by this utility model;

[0025] Figure 6 Top view of the portable fluorescence detection device provided by this utility model Figure 1 ;

[0026] Figure 7 Top view of the portable fluorescence detection device provided by this utility model Figure 2 ;

[0027] Figure 8 A schematic diagram of the sample holder provided by this utility model;

[0028] Figure 9 A top view of the sample holder provided by this utility model;

[0029] Figure 10 A schematic diagram of the optical fiber fixing device provided by this utility model;

[0030] Figure 11 A schematic diagram of the structure of the placement plate provided by this utility model;

[0031] In the figure: 100. Portable fluorescence detection device; 1. Outer shell; 101. Shell body; 102. Placement plate; 103. Mounting hole; 104. Groove; 105. Plate body; 106. Protrusion; 107. First through hole; 108. Cover; 109. Second through hole; 110. First cover plate layer; 111. Second cover plate layer; 112. Epitaxial surface; 2. Laser generator; 3. First optical fiber; 4. Sample holder; 401. First optical fiber hole; 402. 403. Support frame; 404. Test tube rack; 405. Fiber optic fixing plate; 406. Test tube hole; 407. Support plate; 408. Triangular bracket; 409. Handle; 4000. Insertion hole; 5. Second fiber optic cable; 6. Placement slot; 7. Detection hole; 801. Fiber optic fixing device; 802. Second fiber optic cable hole; 803. Fiber optic partition; 804. Support column; 9. Filtering component; 10. Fixing cylinder; 11. Heating device; 12. U-shaped frame; 13. Power supply partition. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0033] The purpose of this invention is to provide a portable fluorescence detection device to solve the problems existing in the prior art. It is low in cost, easy to operate, simple in structure, can be applied in a variety of scenarios, has a wide range of applications, and has high detection accuracy and sensitivity.

[0034] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0035] like Figures 1-11 As shown, this utility model provides a portable fluorescence detection device 100, including a housing 1 and a laser generator 2, a first optical fiber 3, a sample holder 4, and a second optical fiber 5 disposed within the housing 1. There is at least one first optical fiber 3 and one second optical fiber 5. A placement slot 6 is provided on the top of the housing 1 for placing a mobile phone. A detection hole 7 is provided on the bottom wall of the placement slot 6. The sample holder 4 is used to place a sample. The two ends of each first optical fiber 3 are respectively positioned opposite to the output end of the laser generator 2 and the sample. The laser beam emitted by the laser generator 2 can be transmitted to the sample through each first optical fiber 3 and excite the fluorescent substance in the sample to emit fluorescence. The two ends of each second optical fiber 5 are respectively positioned opposite to the sample and the detection hole 7. The fluorescence can be incident on the camera of the mobile phone through each second optical fiber 5 and the detection hole 7. The mobile phone can be used to perform fluorescence detection on the sample.

[0036] This invention emits a laser beam through a laser emitter, which is transmitted to the sample via a first optical fiber 3. This excites the fluorescent material in the sample to produce fluorescence. The fluorescence is then transmitted through a second optical fiber 5 to a detection aperture 7, where a specific light spot is formed at the end of the second optical fiber 5. The beam emitted from this spot passes through the second optical fibers 5 and the detection aperture 7 and is then incident on the camera of a mobile phone (with the phone's camera facing the detection aperture 7), enabling fluorescence detection via a mobile phone. This invention achieves fluorescence detection using only a laser emitter, the first optical fiber 3, the second optical fiber 5, and a mobile phone, eliminating the need for specialized equipment such as PCR amplifiers and spectrometers. It is low-cost, easy to operate, and has a simple structure, making it applicable in various scenarios and with a wide range of applications. The invention simplifies the optical path design by using the first and second optical fibers 3 and 5 for beam transmission, effectively avoiding the influence of external stray light, reducing dissipation, and ensuring detection accuracy and high sensitivity. Furthermore, this invention achieves fluorescence detection via a mobile phone, eliminating the need for additional detectors and further simplifying the structure and reducing costs.

[0037] This invention also includes an optical fiber fixing device 8. The sample holder 4 is provided with at least one first optical fiber hole 401. The end of each first optical fiber 3 near the sample holder 4 is fixedly connected to a first optical fiber hole 401. The end of each second optical fiber 5 near the sample holder 4 is fixedly connected to a first optical fiber hole 401. The optical fiber fixing device 8 is connected to the inner bottom wall of the outer shell 1. The optical fiber fixing device 8 is provided with at least one second optical fiber hole 801. Each second optical fiber hole 801 is arranged opposite to the detection hole 7. The end of each second optical fiber 5 away from the sample holder 4 is fixedly connected to a second optical fiber hole 801. The sample holder 4 fixes the emitting end of the first optical fiber 3 and the incident end of the second optical fiber 5 so that the emitting end of the first optical fiber 3 and the incident end of the second optical fiber 5 can be kept relative to the sample. The optical fiber fixing device 8 fixes the emitting end of the second optical fiber 5 so that the emitting end of the second optical fiber 5 can be opposite to the mobile phone camera, so that the laser beam can be incident on the sample from the emitting end of the first optical fiber 3, and the fluorescence reflected by the fluorescent material in the sample can be incident on the incident end of the second optical fiber 5 and then incident on the mobile phone camera from the emitting end of the second optical fiber 5, so that the mobile phone camera can image the specific light spot.

[0038] In this utility model, the outer shell 1 includes a shell body 101 and a placement plate 102. The placement plate 102 is provided with a detection hole 7. The top of the shell body 101 is provided with a mounting hole 103. At least two grooves 104 are formed on the upper end face of the side wall of the shell body 101, and each groove 104 communicates with the mounting hole 103. The placement plate 102 includes a plate body 105 and at least two protrusions 106. Each protrusion 106 is fixedly connected to the plate body 105. The protrusions 106 are overlapped with a groove 102. The inner bottom wall of the groove 104 allows the plate body 105 to be connected to the mounting hole 103 of the shell body 101, which facilitates the disassembly and installation of the placement plate 102. After removing the placement plate 102, it is convenient to repair and maintain the internal structure of the shell body 101. The upper part of the side wall of the shell body 101 that protrudes above the plate body 105 can form a placement groove 6 with the plate body 105. The inner side wall of the placement groove 6 can restrict the horizontal movement of the mobile phone, prevent the mobile phone from falling, and ensure the stability of the mobile phone when detecting fluorescence.

[0039] In a preferred embodiment, there are two grooves 104 and two protrusions 106, with the two grooves 104 arranged opposite each other and the two protrusions 106 arranged opposite each other.

[0040] This invention also includes a filter component 9 and a fixing cylinder 10. The fixing cylinder 10 is fixedly connected to the lower surface of the placement plate 102 and is positioned opposite to the detection hole 7. The filter component 9 is fixedly connected inside the fixing cylinder 10. The fluorescence emitted from each of the second optical fibers 5 can pass through the filter component 9 and enter the camera of the mobile phone. The filter component 9 can filter out stray light of other wavelengths, ensuring the accuracy of the beam emitted by the light spot in the mobile phone camera. As a preferred embodiment, the filter component 9 can be detachably fixedly connected inside the fixing cylinder 10. The detachable design of the placement plate 102 and the shell body 101 facilitates the replacement of the filter component 9.

[0041] In a preferred embodiment, a snap-fit ​​protrusion is provided inside the fixed cylinder 10, and the filter component 9 is snapped into the fixed cylinder 10 through the snap-fit ​​protrusion, which facilitates the installation of the filter component 9, which is a filter sheet.

[0042] In this invention, the sample holder 4 includes a support frame 402, a test tube rack 403, and an optical fiber fixing plate 404. The test tube rack 403 is fixedly connected to one end of the support frame 402, and has at least one test tube hole 405. The optical fiber fixing plate 404 is fixedly connected to the other end of the support frame 402. The side of the support frame 402 near the optical fiber fixing plate 404 is used to contact the inner bottom wall of the outer casing 1. The optical fiber fixing plate 404 has at least one first optical fiber hole 401, and each test tube hole 405 is opposite to at least one first optical fiber hole 401. The optical fiber is fixed to the bottom of the test tube rack 403, allowing the laser beam to penetrate the entire test tube and obtain as much sample information as possible. In a preferred embodiment, the first optical fiber hole 401 and the test tube hole 405 are coaxial.

[0043] In this invention, the support frame 402 includes a support plate 406 and at least one triangular bracket 407. Each triangular bracket 407 is fixedly connected to one end of the support plate 406, and the test tube rack 403 is disposed at the other end of the support plate 406. The side of the triangular bracket 407 away from the test tube rack 403 and the side of the support plate 406 away from the test tube rack 403 can both contact the inner bottom wall of the outer shell 1. Preferably, there are two triangular brackets 407. The triangular brackets 407 can ensure the stability of the sample rack 4 and prevent the sample rack 4 from swaying or tipping back and forth.

[0044] In this invention, a first through hole 107 is provided on one side wall of the outer shell 1. The outer side wall of the support plate 406 can match the inner bottom wall of the first through hole 107, and each triangular bracket 407, test tube rack 403, and fiber optic fixing plate 404 can pass through the first through hole 107. Sufficient space is left inside the outer shell 1 near the first through hole 107, allowing the support plate 406 to be fitted into the first through hole 107, i.e., the outer side wall of the support plate 406 contacts the inner side wall of the first through hole 107. At this time, the triangular bracket 407, test tube rack 403, and fiber optic fixing plate 404 are located inside the outer shell 1. After the support plate 406 is inserted into the first through hole 107, it can close the first through hole 107, forming a relatively enclosed testing space inside the shell. By pulling the support plate 406 out of the first through hole 107, the test tube rack 403 can be removed for the installation or removal of test tubes. The structure is simple and easy to use.

[0045] In this invention, the test tube rack 403 is detachably connected to the support plate 406. The support rack 402 also includes a handle 408, which is preferably a square handle. The handle 408 is fixedly connected to the side of the support plate 406 away from the test tube rack 403, making it convenient for the user to pull it out to replace the test tube rack 403 or the test tubes in the test tube rack 403.

[0046] This invention also includes a heating device 11, which is mounted on the sample holder 4 and is capable of heating the sample.

[0047] In this invention, the outer shell 1 also includes a cover 108. The laser generator 2 and the sample holder 4 are respectively disposed on both sides inside the outer shell 1. A second through hole 109 is provided on the side wall of the shell body 101 near the laser generator 2. The cover 108 is detachably and fixedly connected to the second through hole 109. Removing the cover 108 allows for convenient disassembly and assembly of components such as the laser generator 2. In a preferred embodiment, the first through hole 107 and the second through hole 109 are arranged opposite to each other.

[0048] This invention also includes a U-shaped frame 12 for placing the laser generator 2. The laser generator 2 is preferably cylindrical, which ensures the stability of the laser generator 2.

[0049] In this invention, a power supply is provided on one side of the U-shaped frame 12. The power supply is detachably connected to the outer casing 1. A power supply partition 13 is provided between the U-shaped frame 12 and the power supply. The power supply partition 13 and the inner side wall of the outer casing 1 opposite to the power supply partition 13 can limit the position of the power supply, ensuring the stability of the power supply placement. Removing the cover 108 allows for convenient disassembly and assembly of the power supply.

[0050] In this invention, the optical fiber fixing device 8 includes an optical fiber partition 802 and at least two support columns 803. Preferably, there are two support columns 803. One end of each support column 803 is fixedly connected to the optical fiber partition 802, and the other end of each support plate 803 is connected to the bottom wall of the outer casing 1. The optical fiber partition 802 has at least one second optical fiber hole 801, preferably multiple second optical fiber holes 801, such as four. The inner bottom wall of the outer casing 1 has at least two mounting slots, and each support column 803 is inserted into one mounting slot to achieve a detachable connection. The structure is simple and easy to assemble and disassemble.

[0051] In this invention, a semi-circular opening is provided on each side of the fiber optic partition 802 to facilitate user access.

[0052] In this invention, the cover 108 includes a first cover layer 110 and a second cover layer 111 connected to each other. The outer edge of the first cover layer 110 protrudes beyond the outer edge of the second cover layer 111, meaning the longitudinal section of the cover 108 is T-shaped. The outer wall of the second cover layer 111 can be interference-fitted with the inner wall of the second through hole 109, meaning the second cover layer 111 can be inserted into the second through hole 109 by friction. The outer shell 1 has an extension surface 112 below the inner bottom wall of the second through hole 109. When the second cover layer 111 is inserted into the second through hole 109, the outer bottom wall of the first cover layer 110 can contact the extension surface 112, and the extension surface 112 can support the first cover layer 110, preventing the cover 108 from falling off under gravity or external force.

[0053] In this invention, the heating device 11 is fixedly connected to the optical fiber fixing plate 404, and the heating device 11 includes at least one heating element. In a preferred embodiment, each heating element corresponds to a test tube. A rectangular window is provided on the side of the test tube rack 403 away from the support plate 406. The optical fiber fixing plate 404 has multiple insertion holes 409 pre-drilled, allowing the pins of each heating element to be inserted into the insertion holes 409 for installation. The heating elements are located on the side of the test tube away from the support plate 406, facilitating their installation and removal. The heating elements can contact or adhere to the outer wall of the test tube to improve the heating effect.

[0054] In a preferred embodiment, each test tube hole 405 corresponds to one first optical fiber hole 401 and one first optical fiber 3, and each first optical fiber 3 corresponds to one second optical fiber 5. There are four test tube holes 405, four first optical fiber holes 401, and four second optical fiber holes 801.

[0055] It should be noted that the phone has built-in fluorescence detection software, which obtains biochemical information by analyzing information such as light spot intensity. The wavelength emitted by laser generator 2 is determined according to the fluorescent substance to be detected, and the emitted laser beam must be able to excite the fluorescent substance to produce fluorescence.

[0056] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A portable fluorescence detection device, characterized by: The utility model provides a portable fluorescence detection device, including shell and the laser generator, first optical fiber, sample holder and second optical fiber of setting in the shell, first optical fiber and second optical fiber are at least one, the top of shell is provided with the placement slot, the placement slot is used for placing cell -phone, the bottom wall of placement slot is provided with detection hole, sample holder is used for placing sample, the both ends of each first optical fiber are opposite with the output of laser generator and sample setting, the laser beam that laser generator emits can be transmitted to sample through each first optical fiber and excite the fluorescence of fluorescent substance in sample to emit, The both ends of each second optical fiber are opposite with sample and detection hole setting, the fluorescence can be through each second optical fiber and detection hole and be incident to the camera of cell -phone, cell -phone can be used for fluorescence detection to sample.

2. The portable fluorescence detection device of claim 1, wherein: It also includes an optical fiber fixing device, the sample holder is provided with at least one first optical fiber hole, each first optical fiber is fixedly connected to one first optical fiber hole near one end of the sample holder, each second optical fiber is fixedly connected to one first optical fiber hole near one end of the sample holder, the optical fiber fixing device is connected with the inner bottom wall of the shell, the optical fiber fixing device is provided with at least one second optical fiber hole, each second optical fiber hole is opposite to the detection hole, and each second optical fiber is fixedly connected to one second optical fiber hole away from one end of the sample holder.

3. The portable fluorescence detection device of claim 1, wherein: The shell includes a shell body and a placement plate, the placement plate is provided with the detection hole, the top of the shell body is provided with a mounting hole, the upper end surface of the side wall of the shell body is provided with at least two grooves, and each groove is communicated with the mounting hole; the placement plate includes a plate body and at least two protrusions, each protrusion is fixedly connected with the plate body, and the plate body can be connected in the mounting hole of the shell body by overlapping each protrusion on the inner bottom wall of one groove, and the part of the upper end of the side wall of the shell body protruding from the plate body can surround the placement slot with the plate body, and the inner side wall of the placement slot can limit the horizontal movement of the cell phone.

4. The portable fluorescence detection device of claim 3, wherein: It also includes a filter component and a fixing cylinder, the fixing cylinder can be fixedly connected with the lower surface of the placement plate, the fixing cylinder is opposite to the detection hole, and the filter component can be fixedly connected in the fixing cylinder, the fluorescence emitted by each second optical fiber can pass through the filter component and be incident into the camera of the cell phone.

5. The portable fluorescence detection apparatus of claim 1, wherein: The sample holder includes a support frame, a test tube holder, and an optical fiber fixing plate, the test tube holder is fixedly connected with one end of the support frame, and the test tube holder is provided with at least one test tube hole; the optical fiber fixing plate is fixedly connected with the other end of the support frame, the side of the support frame close to the optical fiber fixing plate is used to contact the inner bottom wall of the shell, the optical fiber fixing plate is provided with at least one first optical fiber hole, and each test tube hole is opposite to at least one first optical fiber hole.

6. The portable fluorescence detection device of claim 5, wherein: The support frame comprises a support plate and at least one triangular support, each triangular support being fixedly connected to one end of the support plate, and a test tube rack being arranged at the other end of the support plate, and the side of the triangular support away from the test tube rack and the end of the support plate away from the test tube rack are both capable of contacting the inner bottom wall of the shell.

7. The portable fluorescence detection apparatus of claim 6, wherein: A first through hole is arranged on one side wall of the shell, and the outer side wall of the support plate is capable of matching the inner bottom wall of the first through hole, and each triangular support, the test tube rack and the optical fiber fixing plate are capable of passing through the first through hole.

8. The portable fluorescence detection apparatus of claim 1, wherein: The heating device is arranged on the sample rack, and the heating device is capable of heating the sample.

9. The portable fluorescence detection apparatus of claim 3, wherein: The shell further comprises a cover, the laser generator and the sample rack are respectively arranged on both sides in the shell, a second through hole is arranged on the side wall of the shell body close to the laser generator, and the cover is detachably fixedly connected with the second through hole.

10. The portable fluorescence detection apparatus of claim 9, wherein: The cover comprises a first cover plate layer and a second cover plate layer connected with each other, the outer edge of the first cover plate layer protrudes from the outer edge of the second cover plate layer, the outer side wall of the second cover plate layer is capable of interference fit with the inner side wall of the second through hole, the shell is provided with an extension surface below the inner bottom wall of the second through hole, and when the second cover plate layer is inserted into the second through hole, the outer bottom wall of the first cover plate layer is capable of contacting the extension surface. The heating device is arranged on the sample rack, and the heating device is capable of heating the sample.

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