Foot and mouth disease virus-like particle vaccine detection device
By adding a sample top frame and a transparent feeding funnel to the polyacrylamide gel box, the problem of incorrect insertion caused by the compact arrangement of sample slots was solved, achieving efficient and accurate sample addition and saving time and costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-12
- Publication Date
- 2026-04-07
AI Technical Summary
The existing polyacrylamide gel cartridges have a compact arrangement of sample slots, making it easy for pipettes to be inserted into the wrong position, resulting in wasted gel, wasted time, and unnecessary costs.
A sample loading top frame is added to the polyacrylamide gel box, including a transparent loading funnel and a limiting protrusion. The bottom of the transparent loading funnel is equipped with an enlarged structure and an exhaust hole. The operation panel is easy to disassemble and assemble, ensuring accurate sample loading.
It improves the accuracy of sample loading, saves experimental time, avoids unnecessary cost increases, and ensures smooth electrophoretic separation.
Smart Images
Figure CN224095765U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of vaccine development technology, specifically to a foot-and-mouth disease virus-like particle vaccine detection device. Background Technology
[0002] In recent years, virus-like particle vaccines have become an important direction in the research and development of foot-and-mouth disease vaccines. They utilize genetic engineering technology to introduce specific structural protein genes of the foot-and-mouth disease virus into a suitable expression system, allowing them to be expressed and self-assembled to form a structure similar to natural foot-and-mouth disease virus-like particles. Virus-like particle vaccines do not contain the genetic material of the virus and are therefore not infectious.
[0003] When optimizing the purification process of foot-and-mouth disease virus-like particle vaccine, it is necessary to detect the protein purity content of the purified sample. Existing detection methods mostly use the Western blot system, which is based on the principle of extracting proteins from cells, separating them by SDS-PAGE electrophoresis, electrotransferring the separated proteins to a solid membrane, and finally using specific antibodies for an immunoreaction, combined with chemiluminescence detection, and comparing the expression levels of the same protein in multiple samples.
[0004] In the SDS-PAGE electrophoresis separation step, a polyacrylamide gel cartridge is used as the carrier for protein separation. Existing polyacrylamide gel cartridges consist of a polyacrylamide gel, two glass plates on either side of the gel, and a detachable U-shaped clamping frame. Multiple sample slots are located at the top of the polyacrylamide gel. The edges of the gel and the two glass plates are secured within the U-shaped clamping frame. A negative electrode strip is located at the top opening of the clamping frame, and a positive electrode strip is located at the bottom closed end. In use, the polyacrylamide gel cartridge is placed in the electrophoresis pool, buffer solution is poured in, and each protein sample solution is injected into a different sample slot using a pipette. After the power is turned on, the negatively charged protein molecules move towards the bottom. Since proteins of different masses migrate at different rates in the gel, protein separation is achieved. However, the sample slots at the top of the existing polyacrylamide gel are arranged compactly, and the slot openings are small. If the operator is not careful, the pipette tip can easily be inserted into an adjacent sample slot. When two samples are mixed into one sample slot, the gel becomes unusable, resulting in wasted time and unnecessary increased costs.
[0005] In order to solve the above problems, people have been seeking an ideal technological solution. Summary of the Invention
[0006] The purpose of this invention is to address the shortcomings of existing technologies by providing a foot-and-mouth disease virus-like particle vaccine detection device that is easy to operate when adding samples, is less prone to incorrect placement, and thus saves experimental time and avoids unnecessary cost increases.
[0007] To achieve the above objectives, the technical solution adopted by this utility model is: a foot-and-mouth disease virus-like particle vaccine detection device, comprising a polyacrylamide gel box, the polyacrylamide gel box comprising polyacrylamide gel, two glass plates, a detachable U-shaped clamping frame and a sample dispensing top frame, the top of the polyacrylamide gel having a row of sample dispensing slots, the two glass plates being disposed on the front and rear sides of the polyacrylamide gel, the edges of the polyacrylamide gel and the two glass plates being inserted into the U-shaped clamping frame, the tops of the two glass plates being flush, the sample dispensing top frame comprising a frame body and a clamping opening disposed at the bottom of the frame body, the two ends of the frame body extending to the side walls of the U-shaped clamping frame, the clamping opening clamping the outer side of the top of the two glass plates, the frame body having a dispensing funnel in the shape of an inverted quadrangular pyramid corresponding to each sample dispensing slot, the middle of two adjacent dispensing funnels converging to form a sharp edge.
[0008] Based on the above, each of the feeding funnels is a transparent feeding funnel, and each of the feeding funnels has a magnifying structure at its bottom end, which consists of four cylindrical lenses disposed at the bottom outlet edge of the feeding funnel.
[0009] Based on the above, each of the feeding funnels has several vent holes on its wall.
[0010] Based on the above, a limiting protrusion is provided at the inner root of the clamping opening corresponding to the top of the two glass plates.
[0011] Based on the above, the sample loading top frame also includes two operating plates, which are arranged on the front and rear sides of the top of the frame. By squeezing the two operating plates inward, the clamping opening is driven to open.
[0012] This invention has substantial features and advancements compared to existing technologies. Specifically, based on the traditional polyacrylamide gel box, this invention adds a sample loading top frame and sets the tops of the two glass plates to be flush. The feeding funnel maximizes the feeding area, making it easier to insert the pipette tip and less likely to insert it in the wrong position. After the sample loading is completed, the sample loading top frame can be removed, and electrophoretic separation can be performed normally.
[0013] Furthermore, the magnification structure can magnify the sample solution (which is colored) already added to the sample dispensing tank, making it easier for staff to identify and less likely to add the sample to the wrong location.
[0014] Furthermore, by providing several vent holes on the wall of the feeding funnel, air between the top frame of the sample feeding and the buffer solution can be discharged during sample feeding, making the sample feeding smoother.
[0015] Furthermore, the setting of the limiting protrusion facilitates the positioning of the relative position of the sample feeding top frame and the glass plate, ensuring the stable docking position of the feeding funnel and the sample feeding groove; the setting of the two operation plates facilitates the disassembly and assembly of the sample feeding top frame by the staff. Attached Figure Description
[0016] Figure 1 This is a schematic diagram of the front structure of the foot-and-mouth disease virus-like particle vaccine detection device in Example 1.
[0017] Figure 2 This is a schematic diagram of the disassembly and assembly structure of the sample addition top cover in Example 1.
[0018] Figure 3 This is a top view of the sample-adding top cover in Example 1.
[0019] Figure 4 This is an end view of the top cover for adding samples in Example 1.
[0020] Figure 5 This is a schematic diagram of the vertical cross-section of the sample addition funnel in Example 2.
[0021] In the figure: 1. Polyacrylamide gel; 2. Glass plate; 3. U-shaped clamping frame; 4. Sample top frame; 5. Sample groove; 6. Vent hole; 7. Limiting protrusion; 41. Frame body; 42. Clamping port; 43. Feeding funnel; 44. Sharp edge; 45. Operation panel; 46. Cylindrical lens. Detailed Implementation
[0022] The technical solution of this utility model will be further described in detail below through specific embodiments. Example 1
[0023] like Figure 1-4 As shown, a foot-and-mouth disease virus-like particle vaccine detection device includes a polyacrylamide gel box. The polyacrylamide gel box includes a polyacrylamide gel 1, two glass plates 2, and a detachable U-shaped clamping frame 3. A row of sample slots 5 is opened at the top of the polyacrylamide gel 1. The two glass plates 2 are arranged on the front and rear sides of the polyacrylamide gel 1. The edges of the polyacrylamide gel 1 and the two glass plates 2 are inserted into the U-shaped clamping frame 3. This part adopts the common structure of polyacrylamide gel boxes on the market and does not require any creative effort.
[0024] To prevent the pipette tip from being mistakenly inserted into an adjacent sample slot 5 during sample addition, the polyacrylamide gel box also includes a sample addition top frame 4. The tops of the two glass plates 2 are flush. The sample addition top frame 4 includes a frame body 41 and a clamping port 42 located at the bottom of the frame body 41. The two ends of the frame body 41 extend to the side walls of the U-shaped clamping frame 3. The clamping port 42 clamps the outer top of the two glass plates 2. The frame body 41 is provided with an inverted quadrangular pyramid-shaped feeding funnel 43 corresponding to each sample slot 5. The middle of two adjacent feeding funnels 43 converges to form a sharp ridge 44, so that the inlet of the feeding funnel 43 is as large as possible, and the pipette tip can be inserted more easily and is less likely to be inserted incorrectly.
[0025] In practical use, after clamping the two glass plates 2 on both sides of the polyacrylamide gel 1, the edges are clamped with a U-shaped clamping frame 3, and the whole thing is placed into the electrophoresis pool. Buffer solution is injected into the pool, and then the clamping port 42 on the top frame 4 is clamped to the outer top of the two glass plates 2. The bottom outlet of the feeding funnel 43 extends into the sample loading tank 5. The sample solution is drawn up with a pipette, and the pipette tip is inserted into the feeding funnel 43. The tip extends out of the outlet of the feeding funnel 43 under the guidance of the funnel wall. After the sample is loaded, the top frame 4 is removed, and electrophoresis separation can be performed normally.
[0026] To ensure smooth sample addition, in another embodiment, several vent holes 6 can be opened on the wall of each feeding funnel 43 to promptly expel the air between the top frame 4 and the buffer solution during sample addition, thereby preventing internal air pressure from rising and ensuring smooth sample addition.
[0027] To ensure a stable connection between the feeding funnel 43 and the sample loading groove 5, and to prevent them from being too close or too far apart, a limiting protrusion 7 is provided at the inner root of the clamping opening 42 corresponding to the top of the two glass plates 2.
[0028] For ease of operation, the sample loading top frame 4 also includes two operating plates 45. The two operating plates 45 are located on the front and rear sides of the top of the frame 41. The frame 41 is supported between the operating plates 45 and the clamping opening 42. By squeezing the two operating plates 41 inward, the clamping opening 42 is opened, making it easy to assemble and disassemble the sample loading top frame 4. Example 2
[0029] like Figure 5As shown, the difference between this embodiment and Embodiment 1 is that each of the feeding funnels 43 is a transparent feeding funnel, and each feeding funnel 43 has a magnifying structure at its bottom. The magnifying structure consists of four cylindrical lenses 46 disposed at the edge of the outlet at the bottom of the feeding funnel 43. In use, the cylindrical lenses 46 can magnify the sample solution in the sample dispensing tank 5. Since the sample solution is colored, it is easy for the operator to identify whether a sample solution has been added to the sample dispensing tank 5, thus reducing the likelihood of incorrect addition.
[0030] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and not to limit it; although the utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications can still be made to the specific implementation of this utility model or equivalent substitutions can be made to some technical features without departing from the spirit of the technical solution of this utility model, and all such modifications and substitutions should be covered within the scope of the technical solution claimed by this utility model.
Claims
1. A foot-and-mouth disease virus-like particle vaccine detection device, comprising a polyacrylamide gel box, the polyacrylamide gel box including polyacrylamide gel, two glass plates and a detachable U-shaped clamping frame, wherein a row of sample application slots is formed at the top of the polyacrylamide gel, the two glass plates are disposed on the front and rear sides of the polyacrylamide gel, and the edges of the polyacrylamide gel and the two glass plates are inserted into the U-shaped clamping frame, characterized in that: It also includes a sample loading top frame, with the tops of the two glass plates flush. The sample loading top frame includes a frame body and a clamping opening at the bottom of the frame body. The two ends of the frame body extend to the two side walls of the U-shaped clamping frame. The clamping opening is clamped on the outer side of the top of the two glass plates. The frame body is provided with a feeding funnel in the shape of an inverted quadrangular pyramid for each of the sample loading slots. The middle of two adjacent feeding funnels merges into a sharp edge.
2. The foot-and-mouth disease virus-like particle vaccine detection device according to claim 1, characterized in that: Each of the feeding funnels is a transparent feeding funnel, and each of the feeding funnels has a magnifying structure at its bottom, which consists of four cylindrical lenses disposed at the edge of the outlet at the bottom of the feeding funnel.
3. The foot-and-mouth disease virus-like particle vaccine detection device according to claim 2, characterized in that: Each of the feeding funnels has several vent holes on its wall.
4. The foot-and-mouth disease virus-like particle vaccine detection device according to any one of claims 1-3, characterized in that: The inner root of the clamping opening is provided with a limiting protrusion corresponding to the top of the two glass plates.
5. The foot-and-mouth disease virus-like particle vaccine detection device according to claim 4, characterized in that: The sample loading top frame also includes two operating plates, which are located on the front and rear sides of the top of the frame. By squeezing the two operating plates inward, the clamping opening is opened.