Rapid detection test strip for African swine fever virus

Abstract

The utility model provides a rapid test strip for African swine fever virus, which relates to the technical field of biological detection, and comprises a hoop and a test strip, the top of the hoop is provided with a placer, both sides in the placer are provided with bayonets, a bottom sheet is clamped in the placer, the top of the bottom sheet is bonded with the test strip, one side of the top of the test strip is provided with a sample adding port, and the other side of the top of the test strip is provided with a sample outlet. A feeding inner head is embedded in a sample adding opening and is in a circular truncated cone shape, capillary channels are annularly arrayed at the bottom of the feeding inner head, and a hoop is adjustable and worn on a hand, so that a detector can flexibly operate, a detection strip, a liquid storage bag and an arc sleeve are integrated by matching with a bottom piece, and a plurality of parts do not need to be additionally carried. After the sample is collected, the sample and the reaction liquid can be directly mixed in the test strip matching assembly, so that the complicated sample transfer and additional treatment steps are omitted, and the operation process is greatly simplified. And compared with the traditional detection means, the detection time is greatly shortened.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological detection technical field especially, relates to a kind of African swine fever virus rapid detection test strip. BACKGROUND

[0002] As a kind of extremely destructive epidemic disease to pig industry, African swine fever has caused several large-scale epidemics in the global range since first discovery, and has brought immeasurable economic losses to the pig breeding industry of each country. Its characteristics of fast transmission speed and high mortality rate make timely and accurate detection of virus become the key link of epidemic prevention and control. African swine fever virus has complex genomic structure, and can rapidly spread in pig population. Whether it is direct contact with infected pigs or indirect route through contaminated feed, water source, transportation tool, etc., it can cause new outbreak of epidemic. In the process of epidemic prevention and control, screening infected pigs quickly, and taking measures such as isolation and culling, play a decisive role in blocking virus transmission chain and reducing the risk of epidemic spread.

[0003] At present, although the laboratory detection method is accurate, it needs professional equipment and technical personnel, and the detection period is long, which is difficult to meet the demand of on-site rapid screening in breeding farm, slaughterhouse and the like. The existing African swine fever virus detection means has many problems in actual application: 1. Traditional molecular biology detection method, such as PCR technology, needs complex instrument and equipment and professional laboratory environment, and has high technical requirements for operator and high detection cost, so it cannot be used for rapid detection on site; 2. Some existing rapid detection test strips have complicated operation process, sample processing and detection process are separated from each other, which increases detection time and pollution risk, and the structure design of test strip is unreasonable, so it cannot effectively control sample flow rate, resulting in that the accuracy of detection result is affected, so a kind of African swine fever virus rapid detection test strip is needed to solve the above problems. UTILITY MODEL CONTENT

[0004] The utility model aims at solving the shortcomings in the prior art, and provides an African swine fever virus rapid detection test strip.

[0005] In order to achieve the above object, the utility model adopts the following technical scheme: an African swine fever virus rapid detection test strip, comprising a sleeve and a detection strip, a placing device is installed at the top of the sleeve, a bottom plate is connected to the inside of the sleeve through the clamping of the two sides of the inside of the placing device, the top of the bottom plate is bonded with the detection strip, a sample adding port is arranged on one side of the top of the detection strip, a discharging inner head is embedded in the inside of the sample adding port, the shape of the discharging inner head is circular truncated cone, and a capillary channel is arranged in the bottom of the discharging inner head.

[0006] Preferably, the other side of the top of the detection strip is provided with an observation window, the inside of the detection strip is provided with a nitrocellulose membrane, one side of the top of the nitrocellulose membrane is provided with a first detection line, one side of the first detection line is provided with a second detection line, and one side of the second detection line is provided with a quality control line.

[0007] Preferably, the first detection line, the second detection line and the quality control line are located at the observation window.

[0008] Preferably, one side of the bottom plate is embedded with a grommet, the inside of the grommet is provided with a rack, and the surface of the rack is sharp.

[0009] Preferably, one side of the bottom of the bottom plate is bonded with an arc sleeve, and the inside of the arc sleeve is placed with a test tool.

[0010] Preferably, the test tool contains two kinds of cotton swabs and droppers.

[0011] Preferably, the other side of the bottom of the bottom plate is bonded with a liquid storage bag, and the front of the liquid storage bag is screw-connected with a sealing cover.

[0012] Beneficial effects

[0013] In the utility model, the sleeve can be adjusted and worn on the hand, so that the detection personnel can operate flexibly, and the detection strip, the liquid storage bag and the arc sleeve are integrated into one body by matching the bottom plate, so that it is not necessary to carry multiple components additionally. After sample collection, the mixing of the sample and the reaction liquid can be completed in the test paper strip matching assembly, the complex sample transfer and additional processing steps are saved, and the operation process is greatly simplified. Compared with the traditional detection means, the detection time is greatly shortened, and the utility model is especially suitable for places such as breeding farms and slaughterhouses that need to obtain detection results quickly, and the health condition of pigs can be judged in the first time, so that valuable time for timely prevention and control of the epidemic situation is obtained.

[0014] In the utility model, the test paper strip has obvious advantages in detection accuracy and reliability. The capillary pipeline at the bottom of the inner head of the circular table shape blanking in the sample adding port precisely controls the sample flow rate, ensures that the sample uniformly flows into the detection system, and avoids detection errors caused by uneven flow rate. The first detection line and the second detection line arranged on the nitrocellulose membrane detect different antigen sites of African swine fever virus, and the accuracy of the detection result is guaranteed from multiple dimensions in combination with the quality control line. At the same time, reasonable material selection is adopted, for example, the polyethylene material of the liquid storage bag ensures stable storage of the reaction liquid, the components are closely matched, external factor interference is reduced, the pollution risk is reduced, the detection result is more reliable, the precise detection of African swine fever virus is provided with powerful guarantee, and the scientific development of the epidemic situation prevention and control work is effectively assisted. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a schematic view of the detection strip of the utility model;

[0016] Figure 2 It is the bottom sheet structure diagram of the utility model;

[0017] Figure 3 It is the inside schematic diagram of the sample adding port of the utility model;

[0018] Figure 4 It is the bottom sheet structure diagram of the utility model;

[0019] Figure 5 It is the overall structure diagram of the utility model.

[0020] Legend:

[0021] 1, sample adding port; 2, blanking inner head; 3, first detection line; 4, second detection line; 5, observation window; 6, quality control line; 7, nitrocellulose membrane; 8, detection strip; 9, bottom sheet; 10, arc sleeve; 11, test tool; 12, liquid storage bag; 13, sealing cover; 14, capillary channel; 15, embedded ring; 16, rack; 17, sleeve; 18, holder; 19, bayonet. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, purposes and effects realized by the utility model easy to understand, the following further describes the utility model in combination with specific embodiments and drawings, but the following embodiments are only preferred embodiments of the utility model, not all. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative labor all belong to the protection scope of the utility model.

[0023] The specific embodiments of the utility model are described below in combination with the drawings. Specific embodiment one:

[0025] Referring to Figures 1-5 African swine fever virus rapid detection test strip, including sleeve 17 and detection strip 8, the top of sleeve 17 is equipped with holder 18, the inside of holder 18 is equipped with the inside joint of bayonet 19 on both sides, the top of bottom sheet 9 is bonded with detection strip 8, one side of the top of detection strip 8 is equipped with sample adding port 1, the inside of sample adding port 1 is embedded with blanking inner head 2, the shape of blanking inner head 2 is circular truncated cone, and the bottom of blanking inner head 2 is annular array with capillary channel 14.

[0026] As Figure 5 shown in the overall structure diagram, the detection personnel can sleeve 17 on the hand, sleeve 17 bottom is equipped with adjusting port, can adjust size, the detection principle of detection strip 8 here is same with prior art, and bottom sheet 9 is installed in holder 18.

[0027] The other side of the top of the detection strip 8 is provided with an observation window 5, the inside of the detection strip 8 is provided with a nitrocellulose membrane 7, one side of the top of the nitrocellulose membrane 7 is provided with a first detection line 3, one side of the first detection line 3 is provided with a second detection line 4, one side of the second detection line 4 is provided with a quality control line 6. The first detection line 3, the second detection line 4 and the quality control line 6 are all located at the observation window 5.

[0028] One side of the bottom sheet 9 is embedded with a grommet 15, the inside of the grommet 15 is provided with a rack 16, the surface of the rack 16 is sharp. The grommet 15 here is used to cut off the cotton swab.

[0029] One side of the bottom of the bottom sheet 9 is bonded with an arc sleeve 10, the inside of the arc sleeve 10 is placed with a test tool 11. The test tool 11 contains two kinds of cotton swab and dropper. Each bottom sheet 9 integrates the detection strip 8, the arc sleeve 10 and the liquid storage bag 12.

[0030] The other side of the bottom of the bottom sheet 9 is bonded with a liquid storage bag 12, the front of the liquid storage bag 12 is screw-connected with a sealing cover 13. The liquid storage bag 12 is sealed through the sealing cover 13, and the liquid storage bag 12 stores the reaction liquid used for reaction, which is stored in advance. After the biological sample is extracted, the sealing cover 13 is opened, the broken cotton swab is inserted into the liquid storage bag 12, and then it is squeezed to mix. After mixing, the dropper is used to suck from inside and then drop into the sample adding port 1. Specific embodiment two:

[0032] Reference Figures 1-5 The detection personnel puts the sleeve 17 on the hand, adjusts to the appropriate size through the bottom adjusting port. The sleeve 17 is placed in the clamp 18 on the top of the bottom sheet 9, and the detection strip 8 is bonded on the bottom sheet 9. The cotton swab is used to collect the sample of tissue fluid, blood or secretion of the animal suspected to be infected with African swine fever virus, and the sharp rack 16 in the inside surface of the grommet 15 is used to cut off the cotton swab. The sealing cover 13 made of plastic and connected with the liquid storage bag 12 through threads is opened, the broken cotton swab is inserted into the liquid storage bag 12 made of polyethylene, and then it is squeezed to mix the sample with the reaction liquid stored in advance. The reaction liquid contains reagents such as antibodies that can specifically bind with African swine fever virus antigens. After mixing uniformly, the mixed liquid is sucked from the liquid storage bag 12 with the plastic dropper and then dropped into the sample adding port 1 of the detection strip 8. The capillary channel 14 at the bottom of the inner head 2 of the circular truncated cone shape in the sample adding port 1 controls the flow rate of the mixed liquid, so that it flows into the inside of the detection strip uniformly.

[0033] If the sample contains African swine fever virus antigen, it will first combine with specific antibodies labeled with a chromogenic substance (such as colloidal gold) to form an antigen-antibody complex when the mixed solution flows through the nitrocellulose membrane 7 made of nitrocellulose. The complex continues to flow to the first detection line 3 and the second detection line 4, and is captured by the detection line antibody, causing the detection line to develop color. According to the color development of the two detection lines, the content of viral antigen and the infection status can be determined. At the same time, the labeled antibody in the mixed solution also continues to flow to the quality control line 6. If the quality control line develops color, it indicates that the detection process is normal. The tester observes the color development results of the detection line and the quality control line through the observation window 5 made of transparent plastic to determine whether the sample is infected with African swine fever virus. During the entire detection process, the film 9 integrates the test strip 8, the rubber arc sleeve 10 for placing the test tool, and the liquid storage bag 12, making it easy to operate and use.

[0034] In summary:

[0035] 1. In the rapid detection test strip for African swine fever virus, a convenient and efficient on-site detection tool is provided. The tester fixes the detection device on the hand by the cuff 17, and adjusts it to different users by the bottom adjusting port. The cuff 17 is connected to the placement device 18, which integrates the test strip 8, the liquid storage bag 12, and the arc sleeve 10 for placing the test tool. During detection, the cotton swab after sample collection is broken by the inner rack 16 in the grommet 15, mixed with the reaction solution in the liquid storage bag 12, and then sucked by the dropper and dropped into the sample port 1. The flow rate is controlled by the lower inner head 2 bottom capillary tube 14 to flow into the detection system. The detection principle of the test strip is based on the specific binding of antigen-antibody, and the results are determined by the color development of the detection line and the quality control line on the nitrocellulose membrane 7. The overall design is compact and easy to operate, and is suitable for rapid screening of African swine fever virus infection.

[0036] 2. The test strip adopts modular design, and the materials of each component are reasonably selected, such as the liquid storage bag 12 made of polyethylene, the plastic sealing cover 13, the transparent plastic observation window 5, and the rubber arc sleeve 10. Each component works together to realize the integration of sample collection, processing and detection, which not only facilitates carrying, but also simplifies the detection process and reduces human operation errors. At the same time, by setting the first detection line 3 and the second detection line 4, the content of viral antigen and the infection status can be more accurately determined, providing a reliable and practical solution for rapid detection of African swine fever virus, which can be widely used in various scenes that require on-site rapid detection such as farms and quarantine stations.

[0037] In the utility model, unless another definite provision and limitation, first feature is "on" or "under" second feature can include that first and second features are in direct contact, also can include that first and second features are not in direct contact but contact through other feature between them. Moreover, first feature "on", "above" and "on" second feature include that first feature is directly above and obliquely above second feature, or only indicate that first feature horizontal height is higher than second feature. First feature "under", "below" and "under" second feature include that first feature is directly below and obliquely below second feature, or only indicate that first feature horizontal height is less than second feature.

[0038] The basic principle, main features and advantages of the utility model are shown and described above. The skilled in the art should understand that the utility model is not limited by the above examples, the above examples and the description in the specification are only preferred examples of the utility model, and are not used to limit the utility model, various changes and improvements of the utility model can be made without departing from the spirit and scope of the utility model, and these changes and improvements all fall within the scope of the claimed utility model. The scope of protection of the utility model is defined by the appended claims and their equivalents.

Claims

1. A rapid test strip for African swine fever virus, comprising a collar (17) and a test strip (8), characterized in that: The top of the sleeve (17) is equipped with a placer (18). Both sides of the placer (18) have slots (19) that are snapped into the bottom plate (9). The top of the bottom plate (9) is attached with a test strip (8). One side of the top of the test strip (8) has a sample inlet (1). The inside of the sample inlet (1) is embedded with a feeding head (2). The feeding head (2) is shaped like a frustum. The bottom of the feeding head (2) has a capillary tube (14) arranged in a ring.

2. The African swine fever virus rapid detection test strip according to claim 1, characterized in that: The top of the test strip (8) is provided with an observation window (5). A nitrocellulose membrane (7) is installed inside the test strip (8). A first test line (3) is provided on one side of the top of the nitrocellulose membrane (7). A second test line (4) is provided on one side of the first test line (3). A quality control line (6) is provided on one side of the second test line (4).

3. The African swine fever virus rapid detection test strip according to claim 2, characterized in that: The first detection line (3), the second detection line (4), and the quality control line (6) are all located at the observation window (5).

4. The African swine fever virus rapid detection test strip according to claim 1, characterized in that: The substrate (9) has a ring (15) embedded on one side, and the ring (15) has a toothed rack (16) inside, and the surface of the toothed rack (16) is sharp.

5. The African swine fever virus rapid detection test strip according to claim 4, characterized in that: An arc sleeve (10) is glued to one side of the bottom of the film (9), and a testing tool (11) is placed inside the arc sleeve (10).

6. The African swine fever virus rapid detection test strip according to claim 5, characterized in that: The testing tool (11) includes two types: cotton swabs and droppers.

7. The African swine fever virus rapid detection test strip according to claim 5, characterized in that: A liquid storage bag (12) is attached to the other side of the bottom of the substrate (9), and a sealing cap (13) is threaded onto the front of the liquid storage bag (12).

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