Cell separation device for cell detection

Abstract

The utility model discloses a cell separation device for cell detection, which belongs to the technical field of biomedical engineering and comprises a device main body, and the device main body comprises a device body and a cover body; the centrifugal structure comprises a centrifugal tube frame and a piston frame; the centrifugal tube rack comprises a belt turntable and a tube bin; the piston rack comprises a belt turntable and a piston; according to the device, the centrifugal structure is arranged, so that a researcher attaches the cover body to the upper end face of the device body; the started driving piece drives the centrifugal tube to rotate at a high speed, and sample cells in the centrifugal tube are separated under the action of centrifugal force; after primary separation, the researcher separates the cover body from the upper end surface of the device body, so that the piston is separated from the centrifugal tube opening, and the researcher takes out the separated upper-layer cells from the centrifugal tube by using the dropper, so that the operation is simple, the centrifugal tube does not need to be repeatedly taken and poured from a tube bin, and a part of cells are prevented from being adhered to the wall of the centrifugal tube.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical engineering technology, specifically relating to a cell separation device for cell detection. Background Technology

[0002] In the process of cell detection, since biological samples usually contain multiple types of cells, cell separation allows researchers to conduct in-depth analysis of specific cell types. Simply put, cell separation refers to the process of separating target cells from other cells, cell debris, proteins, lipids and other non-cellular components from complex biological samples (such as blood, tissues or other body fluids). This process is also a key step in biomedical applications such as cell analysis, disease diagnosis, and treatment research.

[0003] Researchers typically use cell separation devices to separate cells; these devices utilize the different sedimentation rates of cells in media of different densities to separate cells through centrifugal force. First, researchers place cell samples in centrifuge tubes, and then centrifuge them in the device at a specific speed and time. After centrifugation, the components of different densities form a layered distribution, and the cell layers can be separated.

[0004] However, in practice, after each centrifugation, researchers need to remove the centrifuge tube from the cell separation device and pour it out. Then, they need to pour out the separated upper cell fluid from the centrifuge tube for subsequent testing. After pouring, the centrifuge tube is put back into the cell separation device, and this process is repeated. This operation is not only cumbersome, but during the pouring process, some cells may adhere to the test tube wall, resulting in a reduction in the number of cells and affecting the number of cells and results of subsequent experiments. Therefore, a cell separation device for cell detection is needed to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a cell separation device for cell detection, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a cell separation device for cell detection, comprising:

[0007] The device body includes a device body and a cover, the cover being detachably mounted on the upper surface of the device body;

[0008] A centrifugal structure, comprising a centrifuge tube rack and a piston rack, wherein the centrifuge tube rack is rotatably mounted inside the device body and the piston rack is rotatably mounted on the cover;

[0009] The centrifugal tube rack comprises a rotating disc and a plurality of tube bins, the plurality of tube bins are installed on the rotating disc at intervals, the piston rack comprises a rotating disc and a plurality of pistons, the plurality of pistons are installed on the lower end surface of the rotating disc at intervals, the number of the pistons is the same as that of the tube bins, and the cross-sectional diameter of the piston is smaller than that of the tube bin.

[0010] As a preferred scheme, the side surface of the rotating disc is provided in a gear shape, the centrifugal structure further comprises a driving gear and a driving element, the driving gear is engaged with the rotating disc, the output shaft of the driving element is connected to the driving gear, the driving element is installed inside the device body, and the driving gear is rotatably installed on the lower end of the cover.

[0011] As a preferred scheme, the device body further comprises a supporting disc and a fixing cylinder, the lower end of the fixing cylinder is fixedly installed inside the device body, the supporting disc is slidably arranged on the fixing cylinder, and the supporting disc is attached to the lower end surface of the rotating disc.

[0012] As a preferred scheme, the device body further comprises a rotating screw, the rotating screw is rotatably installed inside the fixing cylinder and coaxially arranged with the fixing cylinder, and the rotating screw is threadedly connected with the supporting disc.

[0013] As a preferred scheme, the centrifugal tube rack is provided with a plug hole at the lower end of the tube bin, the device body further comprises a plurality of fixing columns, the plurality of fixing columns are fixedly installed inside the device body at intervals, and the fixing columns are plugged into the plug holes.

[0014] As a preferred scheme, the device body is provided with a mounting hole, and the cover is provided with a mounting column, the mounting column is plugged into the mounting hole.

[0015] Compared with the prior art, the device has the following beneficial effects:

[0016] The centrifugal structure is arranged, the centrifugal tube rack is rotatably installed inside the device body, and the piston rack is rotatably installed on the cover; the centrifugal tube rack comprises a rotating disc and a plurality of tube bins, the piston rack comprises a rotating disc and a plurality of pistons; the cover is attached to the upper end surface of the device body by the researchers; the centrifugal tube is driven to rotate at high speed by the driving element after being started, and the sample cells in the centrifugal tube are separated under the action of centrifugal force; after one separation, the cover is separated from the upper end surface of the device body by the researchers, so that the piston is separated from the centrifugal tube port, and then the researchers can take out the upper layer cells from the centrifugal tube by using a dropper, which is simple to operate, does not need to reciprocally take and pour the centrifugal tube from the tube bin, and avoids that part of the cells will adhere to the centrifugal tube wall.

[0017] The utility model discloses, with fixed column, centrifugal tube frame has the insertion hole at the lower end part of pipe bin, fixed column has a plurality of, a plurality of fixed column interval fixed installation in the inside of device body, and fixed column is inserted in the insertion hole, after completing centrifugal operation, research personnel rotate the rotation screw, and then drive the support disc along with the rotation screw screw connection axial downward movement of fixed cylinder, under the action of gravity, the belt turntable and pipe bin that adhere to the support disc axial downward movement along fixed cylinder, until fixed column is inserted in the insertion hole, to eject the centrifugal tube from the pipe bin, and research personnel take conveniently. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is the three-dimensional view of the utility model;

[0019] Figure 2 It is the partial explosion drawing of the utility model;

[0020] Figure 3 It is the partial explosion drawing of the centrifugal tube frame and the piston frame of the utility model;

[0021] Figure 4 It is the structure diagram of the device body of the utility model;

[0022] Figure 5 It is the partial explosion drawing of the centrifugal structure of the utility model.

[0023] In the drawing: 1, device body;11, device body;111, mounting hole;12, cover body;121, mounting column;13, support disc;14, fixed cylinder;15, rotation screw;16, fixed column;2, centrifugal structure;21, centrifugal tube frame;211, belt turntable;212, pipe bin;213, insertion hole;22, piston frame;221, piston disc;222, piston;23, driving gear;24, driving part. DETAILED DESCRIPTION

[0024] The utility model will be further described in connection with examples.

[0025] The following examples are used to illustrate the utility model, but cannot be used to limit the protection scope of the utility model. The conditions in the examples can be further adjusted according to specific conditions, and the simple improvement of the method of the utility model under the concept of the utility model belongs to the range of protection required by the utility model.

[0026] Please refer to Figures 1-5 The utility model provides a cell separation device for cell detection, comprising:

[0027] Device body 1, device body 1 includes device body 11 and cover body 12, and cover body 12 detachably installs on the upper end surface of device body 11;

[0028] The centrifugal structure 2 comprises a centrifugal tube rack 21 and a piston rack 22, the centrifugal tube rack 21 is rotatably installed in the device body 11, and the piston rack 22 is rotatably installed on the cover 12;

[0029] The centrifugal tube rack 21 comprises a belt rotating disc 211 and a plurality of tube compartments 212, the plurality of tube compartments 212 are installed on the belt rotating disc 211 at intervals, the piston rack 22 comprises a piston disc 221 and a plurality of pistons 222, the plurality of pistons 222 are installed on the lower end surface of the piston disc 221 at intervals, the number of the pistons 222 is the same as that of the tube compartments 212, and the sectional diameter of the piston 222 is smaller than that of the tube compartment 212;

[0030] The side surface of the belt rotating disc 211 is provided in a gear shape, the centrifugal structure 2 further comprises a driving gear disc 23 and a driving member 24, the driving gear disc 23 is engaged with the belt rotating disc 211, the output shaft of the driving member 24 is connected to the driving gear disc 23, the driving member 24 is installed in the device body 11, and the driving gear disc 23 is rotatably installed on the lower end of the cover 12;

[0031] The device body 1 further comprises a supporting disc 13 and a fixing cylinder 14, the lower end of the fixing cylinder 14 is fixedly installed in the device body 11, the supporting disc 13 is slidably arranged on the fixing cylinder 14, and the supporting disc 13 is attached to the lower end surface of the belt rotating disc 211;

[0032] The device body 1 further comprises a rotating screw 15, the rotating screw 15 is rotatably installed in the fixing cylinder 14 and coaxially arranged with the fixing cylinder 14, and the rotating screw 15 is threadedly connected with the supporting disc 13;

[0033] The centrifugal tube rack 21 is provided with a plug-in hole 213 at the lower end of the tube compartment 212, the device body 1 further comprises a plurality of fixing columns 16, the plurality of fixing columns 16 are fixedly installed in the device body 11 at intervals, and the fixing columns 16 are plugged into the plug-in hole 213;

[0034] The device body 11 is provided with a mounting hole 111, and the cover 12 is provided with a mounting column 121, the mounting column 121 is plugged into the mounting hole 111.

[0035] The working principle and use process of the device are as follows:

[0036] Firstly, the researchers separate the cover 12 from the upper end surface of the device body 11, so that the mounting column 121 is separated from the mounting hole 111; then the centrifugal tube containing sample cells is placed in the tube compartment 212, at this time, the upper end surface of the fixing column 16 is separated from the plug-in hole 213, and the upper end surface of the supporting disc 13 is attached to the lower end surface of the belt rotating disc 211;

[0037] Then, the researchers will install the column 121 plug in the installation hole 111 inside, so that the cover 12 on the device body 11 upper end face, at this time installed in the lower end of the piston 222 plug in the centrifuge tube mouth tube warehouse 212; after the start of the drive member 24 drive the driving gear 23 rotation, so that the driving gear 23 with the belt rotation disc 211 is also rotated, in turn, the tube warehouse 212 and tube warehouse 212 in the centrifuge tube high speed rotation, under the action of centrifugal force, the sample cells in the centrifuge tube is separated;

[0038] After a separation, researchers will again cover 12 from the device body 11 upper end face, so that the piston 222 from the centrifuge tube mouth, then researchers use pipette will be separated after the upper layer of cells from the centrifuge tube can be taken out, simple operation, no need to take and pour the centrifuge tube from the tube warehouse 212 reciprocating, avoid part of the cells may be adhered to the centrifuge tube wall and cause the number of cells to reduce;

[0039] In addition, after the completion of the centrifugation operation, the researchers rotate the rotation screw 15, in turn, drive the support disc 13 with the rotation screw 15 thread connection along the fixed cylinder 14 axial downward movement, under the action of gravity, the cover plate 13 with the belt rotation disc 211 and tube warehouse 212 along the fixed cylinder 14 axial downward movement, until the fixed column 16 plug in the plug hole 213, in order to eject the centrifuge tube from the tube warehouse 212, convenient researchers to take.

[0040] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A cell separation device for cell detection, characterized by, The utility model relates to a centrifugal device, including: Device body (1), device body (1) includes device ontology (11) and cover (12), cover (12) detachably installed on device ontology (11) upper end surface; Centrifugal structure (2), centrifugal structure (2) includes centrifugal tube frame (21) and piston frame (22), centrifugal tube frame (21) rotatably installed in device ontology (11) inside, piston frame (22) rotatably installed on cover (12); Wherein, centrifugal tube frame (21) includes belt turntable (211) and tube warehouse (212), tube warehouse (212) are provided with multiple, multiple tube warehouse (212) are installed at belt turntable (211) interval, piston frame (22) includes piston disc (221) and piston (222), piston (222) are provided with multiple, multiple piston (222) are installed at piston disc (221) lower end surface interval, the number of piston (222) and tube warehouse (212) is same, and the cross section diameter of piston (222) is less than the cross section diameter of tube warehouse (212).

2. The cell separation device for cell detection according to claim 1, wherein: The side of the belt turntable (211) is provided as a gear, the centrifugal structure (2) further includes a driving gear (23) and a driving member (24), the driving gear (23) is engaged with the belt turntable (211), the output shaft of the driving member (24) is connected to the driving gear (23), the driving member (24) is installed inside the device body (11), and the driving gear (23) is rotatably installed at the lower end of the cover (12).

3. The cell separation device for cell detection according to claim 2, wherein: The device body (1) further includes a support disc (13) and a fixed cylinder (14), the lower end of the fixed cylinder (14) is fixedly installed inside the device body (11), the support disc (13) is slidably provided in the fixed cylinder (14), and the support disc (13) is attached to the lower end surface of the belt turntable (211).

4. The cell separation device for cell detection according to claim 3, wherein: The device body (1) further includes a rotating screw (15), the rotating screw (15) is rotatably installed inside the fixed cylinder (14) and coaxially arranged with the fixed cylinder (14), and the rotating screw (15) is threadedly connected with the support disc (13).

5. The cell separation device for cell detection according to claim 4, wherein: The centrifugal tube frame (21) has a plug-in hole (213) at the lower end of the tube warehouse (212), and the device body (1) further includes a plurality of fixed columns (16), the plurality of fixed columns (16) are fixedly installed inside the device body (11) at intervals, and the fixed columns (16) are plugged into the plug-in holes (213).

6. The cell separation device for cell detection according to claim 5, wherein: The device body (11) has a mounting hole (111), and the cover (12) has a mounting column (121) plugged into the mounting hole (111).

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