Plasma in-vitro cell processing device and plasma in-vitro cell processing system
By using a connector and conical cover design, combined with a partition plate and a columnar body, the problem of plasma nozzles blowing away cells was solved, achieving uniform contact and safe handling of plasma with cells, and improving the effectiveness of cell experiments.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
- Filing Date
- 2025-05-12
- Publication Date
- 2026-04-14
AI Technical Summary
The nozzle design of existing plasma therapy devices can easily blow away cells in cell culture dishes, making it impossible to effectively control the contact area and intensity of plasma with cells, thus limiting the application effect of plasma in cell experiments.
It adopts a connector and conical cover design. The connector is connected to the plasma therapy device, and the conical cover gradually expands its inner diameter to form a fan-shaped airflow. Combined with the partition plate and columnar body, it controls the diffusion and intensity of the plasma airflow and distributes the plasma airflow evenly through perforations.
Effective control of the contact area and intensity between plasma and cells reduces the risk of cells being blown away, improves the safety and efficiency of the process, and enhances the application effect of plasma in cell experiments.
Smart Images
Figure CN224119005U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of oral medicine, specifically to a plasma extracorporeal cell processing device and a plasma extracorporeal cell processing system. Background Technology
[0002] Currently, to study the effects of plasma on cells, plasma therapy devices are typically used to blow plasma into cells outside the body.
[0003] In related technologies, in vitro cells are placed in cell culture dishes and then plasma is sprayed into each in vitro cell. However, the nozzle design of the plasma therapy device is prone to blowing away the cells in the cell culture dish, and it is impossible to effectively control the contact area and intensity of the plasma with the cells, thus limiting the application effect of plasma in cell experiments. Summary of the Invention
[0004] This application provides a plasma in vitro cell processing device and a plasma in vitro cell processing system, which can solve the technical problems in related technologies where the nozzle design of plasma therapy instruments easily blows away cells in cell culture dishes and cannot effectively control the contact area and intensity of plasma with cells, thus limiting the application effect of plasma in cell experiments.
[0005] In a first aspect, embodiments of this application provide a plasma in vitro cell treatment device, comprising: a connector, one end of which is used to connect to a plasma therapy device; and a conical cover connected to the other end of the connector, the end of the conical cover away from the connector being used to cover the opening of a cell culture dish, wherein the inner diameter of the end of the conical cover near the connector is smaller than the inner diameter of the end of the conical cover away from the connector.
[0006] In conjunction with the first aspect, in one embodiment, the plasma in vitro cell processing device further includes: a partition plate, the partition plate being installed on the side of the conical cover away from the connector, and the partition plate being used to cover the opening of the cell culture dish, the partition plate having multiple perforations so that the conical cover and the cell culture dish are connected through the multiple perforations.
[0007] In conjunction with the first aspect, in one embodiment, the conical cover is further connected to a columnar body, the columnar body being connected to the end of the conical cover away from the connector, and the partition plate being installed on the inner sidewall of the columnar body.
[0008] In conjunction with the first aspect, in one embodiment, the columnar body is provided with scale lines that extend along the axial direction of the columnar body.
[0009] In conjunction with the first aspect, in one embodiment, each of the perforations has the same inner diameter, and the inner diameter of each perforation is set to 1 to 3 mm.
[0010] In conjunction with the first aspect, in one embodiment, the connector includes: a connecting segment for connecting to a plasma therapy device; and a buffer segment connected to the connecting segment, wherein one end of the buffer segment away from the connecting segment is connected to the conical cover, and the outer diameter of the connecting segment is smaller than the outer diameter of the buffer segment.
[0011] In conjunction with the first aspect, in one embodiment, the buffer section has a conical cavity that extends along the extension direction of the buffer section, and the inner diameter of the conical cavity near the end of the connecting section is smaller than the inner diameter of the conical cavity near the end of the conical cover.
[0012] In conjunction with the first aspect, in one embodiment, the inner wall of the buffer section is connected to the inner wall of the conical cover via an arc-shaped section.
[0013] In conjunction with the first aspect, in one embodiment, both the connector and the conical cover are made of acrylic material.
[0014] Secondly, embodiments of this application provide a plasma in vitro cell processing system, which includes the plasma in vitro cell processing device as described above. The plasma in vitro cell processing system further includes: a cell culture dish having an opening, and the end of the conical cover away from the connector covering the opening.
[0015] The beneficial effects of the technical solutions provided in this application include:
[0016] By using a connector to connect the handle of the plasma therapy device, and by gradually increasing the inner diameter of the conical cover from the connection point with the connector towards the cell culture dish, the plasma output from the plasma therapy device enters the connector and then enters the conical cover. The plasma gas flow inside the conical cover gradually diffuses along the inner wall of the conical cover, forming a fan-shaped gas flow with a larger coverage area. This effectively reduces the impact force of the gas flow on the cells, solving the technical problem in related technologies where it is impossible to effectively control the contact area and intensity of plasma with cells, thus limiting the application effect of plasma in cell experiments. Attached Figure Description
[0017] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0018] Figure 1 This is a schematic cross-sectional view of the plasma in vitro cell processing device provided in the embodiments of this application;
[0019] Figure 2 This is a bottom view of the plasma in vitro cell processing device provided in the embodiments of this application;
[0020] Figure 3 This is a schematic diagram of the main structure of the plasma in vitro cell processing device provided in the embodiments of this application.
[0021] In the picture:
[0022] 1. Connector; 11. Connecting section; 12. Buffer section; 121. Conical cavity;
[0023] 2. Conical cover;
[0024] 3. Divider; 31. Perforation;
[0025] 4. Columnar body;
[0026] 5. Arc-shaped segment. Detailed Implementation
[0027] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present application.
[0028] This application provides a plasma in vitro cell treatment device, which solves the technical problem in related technologies where the nozzle design of plasma therapy devices easily blows away cells in cell culture dishes and cannot effectively control the contact area and intensity of plasma with cells, thus limiting the application effect of plasma in cell experiments.
[0029] See Figure 1The diagram illustrates a plasma in vitro cell treatment device provided in this application embodiment. It may include: a connector 1, one end of which is connected to a plasma therapy device; and a conical cover 2 connected to the other end of the connector 1. The end of the conical cover 2 away from the connector 1 is used to cover the opening of a cell culture dish. The inner diameter of the end of the conical cover 2 near the connector 1 is smaller than the inner diameter of the end of the conical cover 2 away from the connector 1. It should be understood that the connector 1 can be connected to the handle of the plasma therapy device, which has an outlet for plasma gas flow. The connector 1 has a cavity communicating with this outlet. The cavity of the conical cover 2 is also connected to the outlet at the handle through the cavity of the connector 1. The plasma therapy device injects plasma gas from the handle into the connector 1, then into the conical cover 2, and finally into the cell culture dish. The inner diameter of the conical cover 2 near the connector 1 is smaller than the inner diameter of the conical cover 2 away from the connector 1. That is, the inner diameter of the conical cover 2 gradually increases from the connection point with the connector 1 toward the end away from the connector 1, and the area of the cross-section also gradually increases, similar to a funnel shape.
[0030] This embodiment utilizes a connector 1 to connect to the handle of a plasma therapy device, allowing the plasma therapy device to input plasma gas flow from the handle into the connector 1. The plasma gas flow then enters the conical enclosure 2, which is connected to the connector 1. Since the inner diameter of the conical enclosure 2 gradually increases from the connection point with the connector 1 towards the cell culture dish, the end of the conical enclosure 2 near the cell culture dish can accommodate the size of the cell culture dish. The plasma gas flow entering the conical enclosure 2 from the connector 1 can gradually diffuse along the inner wall of the conical enclosure 2, forming a fan-shaped gas flow with a larger coverage area. This allows the gas flow to gradually diffuse and slow down after entering the conical enclosure 2, ultimately acting on the surface of in vitro cells in a relatively gentle manner. This effectively reduces the impact force of the gas flow on the cells and reduces the risk of in vitro cells being blown away, greatly improving the safety and effectiveness of in vitro cell treatment. It enhances the safety and activity of in vitro cells during treatment and solves the technical problem in related technologies where the contact area and intensity of plasma with cells cannot be effectively controlled, thus limiting the application effect of plasma in cell experiments.
[0031] See Figure 2As shown, in some optional embodiments, the plasma in vitro cell processing device may further include: a partition plate 3, which is installed on the side of the conical cover 2 away from the connector 1, and is used to cover the opening of the cell culture dish. The partition plate 3 has a plurality of perforations 31, allowing the conical cover 2 and the cell culture dish to communicate through the perforations 31. Preferably, the plurality of perforations 31 are evenly distributed on the partition plate 3, and the axial directions of each perforation 31 are parallel to each other. By setting a separator plate 3, the plasma gas flow inside the conical enclosure 2 passes through the perforations 31 on the separator plate 3 before entering the cell culture dish. This further disperses the plasma gas flow into multiple tiny gas flow beams. Specifically, the plasma gas flow forms a fan-shaped gas flow with a larger coverage area inside the conical enclosure 2 before acting on the separator plate 3. The evenly distributed perforations 31 on the separator plate 3 further disperse the plasma gas flow into multiple tiny plasma gas flow beams. These plasma gas flow beams impact the cell culture dish at a smaller angle, thereby reducing the impact force on the in vitro cells inside the cell culture dish and further improving the efficiency of plasma treatment of in vitro cells. This not only enhances the safety and activity of in vitro cells during the treatment process but also allows the plasma to act more evenly on the surface of in vitro cells, which is beneficial for studying the various effects of plasma on cells and their mechanisms of action.
[0032] In some optional embodiments, the conical cover 2 is further connected to a columnar body 4, which is connected to the end of the conical cover 2 away from the connector 1. The partition plate 3 is installed on the inner wall of the columnar body 4. In this embodiment, the columnar body 4 is configured as a cylinder with the same inner diameter along its axial direction. The edge of the partition plate 3 can be connected to the inner wall of the columnar body 4, and the connection can be made by light-curing adhesive. When the partition plate 3 is placed over the opening of the cell culture dish, part of the inner wall of the columnar body 4 can surround the periphery of the cell culture dish. The outer wall of the cell culture dish can be at a certain distance from the inner wall of the columnar body 4, so that the conical cover 2 and the columnar body 4 can be removed from the cell culture dish after the experiment. By setting the columnar body 4, the cell culture dish can be completely covered, and the bottom surface of the columnar body 4 can directly contact the operating table, enhancing the overall stability of the plasma in vitro cell processing device. It should be noted that the separator 3 can be in direct contact with the top surface of the cell culture dish and cover the opening of the cell culture dish, or it can have a certain gap with the top surface of the cell culture dish.
[0033] In some optional embodiments, the column 4 is provided with graduation lines that extend along the axial direction of the column 4. By setting graduation lines, the height of the cell culture dish can be measured before the experiment begins, and then the separator 3 can be installed at a suitable height on the column 4 based on the measured height of the cell culture dish, reducing the need for repeated adjustments during the experiment.
[0034] In some optional embodiments, each of the perforations 31 has the same inner diameter, and the inner diameter of each perforation 31 is set to 1–3 mm. Setting the inner diameter of the perforations 31 to 1–3 mm ensures that the plasma can form a stable laminar flow when passing through the perforations 31, enhancing the safety of the plasma acting on in vitro cells and reducing the possibility of cell damage. The uniform distribution of the perforations 31 with the same inner diameter also ensures uniform stress distribution on the separator 3, improving the uniformity of cell treatment.
[0035] In some optional embodiments, the connector 1 may include: a connecting segment 11 for connecting to a plasma therapy device; and a buffer segment 12 connected to the connecting segment 11, with one end of the buffer segment 12 away from the connecting segment 11 connected to the conical cover 2. The outer diameter of the connecting segment 11 is smaller than the outer diameter of the buffer segment 12. The smaller outer diameter of the connecting segment 11 allows the connecting segment 11 to extend into the handle outlet of the plasma therapy device, enabling rapid connection and disconnection between the connecting segment 11 and the handle. The larger outer diameter of the buffer segment 12 prevents over-insertion, enhancing the safety of the plasma extracorporeal cell treatment device.
[0036] See Figure 3 As shown, in some optional embodiments, the buffer section 12 has a conical cavity 121 extending along the extension direction of the buffer section 12, and the inner diameter of the conical cavity 121 near the end of the connecting section 11 is smaller than the inner diameter of the conical cavity 121 near the end of the conical cover 2. It should be understood that the conical cavity 121 here can refer to the inner wall of the buffer section 12 forming a truncated cone shape, with a small change in inner diameter between the connection point with the connecting section 11 and the connection point with the conical cover 2. This allows the plasma gas flow to be buffered for a certain distance within the buffer section 12 before expanding into the conical cover 2, achieving the purpose of phased control of the plasma gas flow, realizing energy gradient management of the plasma gas flow, and improving the transmission performance of the plasma gas flow.
[0037] Preferably, the inner wall of the buffer section 12 is connected to the inner wall of the conical cover 2 via an arc-shaped section 5. That is, when the plasma gas flow passes through the connection between the buffer section 12 and the conical cover 2, the arc-shaped section 5 can provide a certain buffer, allowing the plasma gas flow to have a transition process before entering the conical cover 2 from the buffer section 12, so that the plasma gas flow can flow more smoothly.
[0038] In some optional embodiments, both the connector 1 and the conical cover 2 are made of acrylic. Additionally, the columnar body 4 can also be made of acrylic. Acrylic has high hardness, high transparency, and good plasticity, making it very suitable for processing using 3D printing technology. That is, in this embodiment, the connector 1, conical cover 2, and columnar body 4 can be integrally formed by 3D printing. The high hardness of acrylic ensures that the connector 1, conical cover 2, and columnar body 4 maintain their shape and dimensional stability during use, and will not deform due to external forces or temperature changes. Its transparency allows researchers to directly observe the flow of plasma gas inside the connector 1, conical cover 2, and columnar body 4, as well as the contact effect with the cell culture dish, facilitating timely adjustment of experimental parameters. Simultaneously, the high plasticity of acrylic facilitates subsequent possible structural optimization and functional expansion, allowing for local modifications or the addition of special functional components according to experimental needs. In some other embodiments, the connector 1, conical cover 2, and columnar body 4 can also be made of resin.
[0039] This application also provides a plasma in vitro cell processing system, which may include the plasma in vitro cell processing device as described in the preceding lines. The plasma in vitro cell processing system further includes: a cell culture dish having an opening, and the conical cover 2 covering the opening at one end away from the connector 1. The plasma in vitro cell processing system includes the plasma in vitro cell processing device of any of the above embodiments, and will not be described again here.
[0040] In the description of this application, it should be noted that the terms "upper," "lower," etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. Unless otherwise expressly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication between two elements. For those skilled in the art, the specific meaning of the above terms in this application can be understood according to the specific circumstances.
[0041] It should be noted that in this application, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.
[0042] The above description is merely a specific embodiment of this application, enabling those skilled in the art to understand or implement this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features claimed herein.
Claims
1. A plasma-based extracorporeal cell treatment device, characterized in that, It includes: Connector (1), one end of which is used to connect to a plasma therapy device; A conical cover (2) is connected to the other end of the connector (1). The end of the conical cover (2) away from the connector (1) is used to cover the opening of the cell culture dish. The inner diameter of the end of the conical cover (2) near the connector (1) is smaller than the inner diameter of the end of the conical cover (2) away from the connector (1).
2. The plasma extracorporeal cell treatment device as described in claim 1, characterized in that, The plasma in vitro cell treatment device also includes: A partition plate (3) is installed on the side of the conical cover (2) away from the connector (1), and the partition plate (3) is used to cover the opening of the cell culture dish. The partition plate (3) has multiple perforations (31) so that the conical cover (2) and the cell culture dish are connected through the multiple perforations (31).
3. The plasma extracorporeal cell treatment device as described in claim 2, characterized in that: The conical cover (2) is also connected to a columnar body (4), which is connected to the end of the conical cover (2) away from the connector (1), and the partition plate (3) is installed on the inner side wall of the columnar body (4).
4. The plasma extracorporeal cell treatment device as described in claim 3, characterized in that: The column (4) is provided with scale lines, which extend along the axial direction of the column (4).
5. The plasma extracellular cell treatment device as described in claim 2, characterized in that: Each of the perforations (31) has the same inner diameter, and the inner diameter of each perforation (31) is set to 1 to 3 mm.
6. The plasma in vitro cell treatment device as described in claim 1, characterized in that, The connector (1) includes: Connection segment (11), the connection segment (11) is used to connect to the plasma therapy device; A buffer section (12) is connected to the connecting section (11), and one end of the buffer section (12) away from the connecting section (11) is connected to the conical cover (2). The outer diameter of the connecting section (11) is smaller than the outer diameter of the buffer section (12).
7. The plasma extracellular cell treatment device as described in claim 6, characterized in that: The buffer section (12) has a conical cavity (121) that extends along the extension direction of the buffer section (12), and the inner diameter of the conical cavity (121) near the end of the connecting section (11) is smaller than the inner diameter of the conical cavity (121) near the end of the conical cover (2).
8. The plasma extracellular cell treatment device as described in claim 6, characterized in that: The inner wall of the buffer section (12) is connected to the inner wall of the conical cover (2) through an arc section (5).
9. The plasma extracellular cell treatment device as described in claim 1, characterized in that: Both the connector (1) and the conical cover (2) are made of acrylic material.
10. A plasma extracorporeal cell treatment system, characterized in that, It includes the plasma in vitro cell treatment device as described in any one of claims 1 to 9, and the plasma in vitro cell treatment system further includes: A cell culture dish having an opening, wherein the conical cover (2) is positioned over the opening at one end away from the connector (1).