Erythrocyte osmotic brittleness colorimetric experiment analyzer

Abstract

The utility model discloses a red blood cell osmotic brittleness colorimetric experiment analyzer which comprises a working stand, a centrifugal assembly used for separating red blood cells is fixedly arranged on the upper surface of the working stand, a solution assembly used for placing solutions with different concentrations is fixedly arranged on the upper surface of the working stand, the solution assembly comprises a solution box, and a mixing assembly comprises a limiting disc. The mounting block is fixedly mounted on the upper surface of the sliding block, one side of the drawing block and the telescopic end of the telescopic motor are mounted through a bolt, the drawing end of the negative pressure suction pipe and the drawing block are fixedly mounted, the negative pressure suction pipe is arranged on the upper surface of the reagent bottle in a sliding mode, and the negative pressure suction pipe extracts solutions in the reagent bottle and puts the solutions in the mixing test tube. The negative pressure suction tube is driven by the telescopic motor to move between the reagent bottle and the mixing test tube, and a required amount of solution is sucked under the action of negative pressure; and then the solution is moved above the mixing test tube, and the solution is released into the test tube, so that powerful support is provided for the erythrocyte osmotic brittleness experiment, and errors caused by manual operation are reduced.

Description

Technical Field

[0001] The embodiments of this utility model belong to the field of medical device technology, and more specifically, relate to a colorimetric analyzer for red blood cell osmotic fragility testing. Background Technology

[0002] Erythrocyte osmotic fragility, or the tensile strength of the erythrocyte membrane in a hypotonic solution, is a key indicator in clinical practice. The reduction of membrane cholesterol may be the root cause of increased cell membrane fragility. The measurement of erythrocyte osmotic fragility is an important reference indicator in the clinical practice of microcytic anemias such as thalassemia and iron deficiency anemia.

[0003] The current principle of the erythrocyte osmotic fragility test is as follows: RBCs are placed in a hypotonic solution, and water enters the RBCs, causing them to swell. When the swelling reaches a certain extent, the RBCs will rupture and dissolve. Since the solubility of RBCs in hypotonic sodium chloride solutions of different concentrations varies depending on the disease, some are easily dissolved while others are difficult to dissolve, the erythrocyte osmotic fragility test assists in the clinical diagnosis of corresponding diseases. The main steps of the traditional method are: accurately prepare 12 tubes of sodium chloride solution with different concentrations of 0.68%, 0.64%, 0.60%, 0.56%, 0.52%, 0.48%, 0.44%, 0.40%, 0.36%, 0.32%, 0.28%, and 0.24%, 1 ml in each tube. Draw about 1.5 ml of heparin lithium anticoagulated blood from the patient and immediately drip it into each tube, one drop per tube. Shake gently and let stand at room temperature for 2 hours to observe the results. Observation results: Starting with the high concentration tube, the tube that begins to show a transparent red color in the upper layer and has red blood cells at the bottom is the tube that has begun to dissolve; the tube that is transparent red and has no red blood cells at the bottom is the tube that has completely dissolved.

[0004] The interpretation of results using existing methods relies on visual observation and comparison with distilled water and saline tubes. This observation method is easily affected by various factors such as differences in the observer's vision, lighting conditions, and observation angle, leading to subjectivity in result interpretation and potential errors. The manual method requires accurately preparing multiple tubes of sodium chloride solution with different concentrations, adding patient blood samples one by one, and then allowing them to stand for observation. This process is cumbersome, time-consuming, and not conducive to obtaining experimental results quickly.

[0005] The use of contrast color experiments requires a large amount of reagents. The amount of NaCl solution and red blood cell suspension of different concentrations is difficult to control precisely when operating manually. Small changes in concentration may lead to significant differences in experimental results, which not only increases experimental costs but may also have a certain impact on the environment. Utility Model Content

[0006] To address the aforementioned deficiencies or improvement needs of existing technologies, this utility model provides a colorimetric analyzer for red blood cell osmotic fragility, comprising a work stand, a centrifugation component for separating red blood cells fixedly mounted on the upper surface of the work stand, a solution component for holding solutions of different concentrations fixedly mounted on the upper surface of the work stand, a mixing component for mixing red blood cells and solutions fixedly mounted on the upper surface of the work stand, a pipetting component for aspirating reagents fixedly mounted on one side of the mixing component, a sample-grabbing component provided on one side of the pipetting component, an electrically controlled control component fixedly mounted on one side of the upper surface of the work stand, and a spectrophotometric component fixedly mounted on the upper surface of the work stand;

[0007] The solution assembly includes a solution box containing several sets of reagent bottles. The mixing assembly includes a limiting plate containing several sets of mixing test tubes. The pipetting assembly includes a fourth support frame, a linear guide plate, a ball screw, a slider, a mounting block, a telescopic motor, a pull block, and a negative pressure pipette. The fourth support frame is fixedly mounted on the upper surface of the workbench. The linear guide plate is fixedly mounted between the fourth support frames. The ball screw is fixedly mounted in the middle of the linear guide plate. The slider is slidably mounted on the outside of the ball screw. The mounting block is fixedly mounted on the upper surface of the slider. The telescopic motor is bolted to the surface of the mounting block. One side of the pull block is bolted to the telescopic end of the telescopic motor. The pull end of the negative pressure pipette is fixedly mounted to the pull block. The negative pressure pipette is slidably positioned on the upper surface of the reagent bottles. The negative pressure pipette draws solution from the reagent bottles and places it inside the mixing test tubes.

[0008] Preferably, the clamping assembly includes a fifth support frame, a fixed plate, a first telescopic rod, a slide rod, a triangular plate, a slide rail, a fixed clamping rod, a movable clamping rod, and a second telescopic rod. The fifth support frame is bolted to the upper surface of the workbench. The two ends of the fixed plate are fixedly installed at the top of the fifth support frame. The first telescopic rod is fixedly installed in the middle of the fixed plate. Two sets of slide rods are fixedly installed at both ends of the fixed plate. The triangular plate is fixedly installed at the telescopic end of the first telescopic rod. The two ends of the triangular plate are sleeved with the slide rod. A U-shaped plate is fixedly installed on the upper surface of the triangular plate. The slide rail is bolted to the top wall of the U-shaped plate. The fixed clamping rod is fixedly installed on the surface of one set of slide rails. The movable clamping rod is slidably installed on the surface of another set of slide rails. The driving end of the movable clamping rod is fixedly installed with the movable clamping rod.

[0009] Preferably, the hybrid assembly further includes a third support frame, a third inclined plate, and a second servo motor. The third support frame is fixedly installed on the upper surface of the workbench, the third inclined plate is fixedly installed between the third support frames, the second servo motor is fixedly installed on the bottom wall of the third inclined plate, the drive end of the second servo motor is provided with a pulley, and the bottom end of the limiting plate is fixed with a rotating shaft, which is rotatably connected to the pulley.

[0010] Preferably, the solution assembly further includes a second support frame, the bottom wall of which is fixedly installed to the upper surface of the work stand, and the bottom end of which is fixedly installed to the solution box, the solution box being in an inclined state.

[0011] Preferably, the spectrophotometric assembly includes a mounting box, a spectrophotometer, and cuvettes. The top surface of the mounting box is a hinged cover. The spectrophotometer is disposed inside the mounting box, and several sets of cuvettes are installed in parallel inside the mounting box.

[0012] Preferably, the centrifuge assembly includes a first support frame, a first inclined plate, a first servo motor, and a centrifuge disc. The first support frame is bolted to one side of the workbench. The first inclined plate is disposed between the first support frames. The first servo motor is fixedly installed on the bottom wall of the first inclined plate. The driving end of the first inclined plate is fixedly connected to the middle of the centrifuge disc. Several sets of centrifuge tubes are placed inside the centrifuge disc.

[0013] Preferably, the first servo motor, the second servo motor, the telescopic motor, the ball screw motor, and the first telescopic rod are all electrically connected to the control component.

[0014] In summary, compared with the prior art, the above-described technical solution conceived by this utility model can achieve the following beneficial effects:

[0015] (1) The pipetting assembly of this utility model uses a telescopic motor to drive a negative pressure pipette to move between a reagent bottle and a mixing tube. When the negative pressure pipette moves above the reagent bottle, it draws the required amount of solution through negative pressure. Then it moves above the mixing tube and releases the solution into the tube. This process can be repeated to achieve precise addition and mixing of solutions of different concentrations. The spectrophotometer is used to measure the absorbance of the solution in the mixing tube to assess the osmotic fragility of red blood cells. These components integrate multiple functional modules such as centrifugation, solution management, mixing, pipetting, clamping, and spectrophotometry, which simplifies the experimental process, provides strong support for red blood cell osmotic fragility experiments, and reduces errors caused by human operation. The integration of multiple functional modules simplifies the experimental process and improves the accuracy and efficiency of the experiment.

[0016] (2) This utility model uses a centrifugation assembly to centrifuge red blood cell samples. Centrifugation is an important step in the red blood cell osmotic fragility experiment. It can help red blood cells to be effectively separated from the solution, thereby more accurately assessing the osmotic fragility of red blood cells. The solution assembly includes a solution box and several reagent bottles. The solution box is used to store and manage solutions of different concentrations. These solutions are used to mix with red blood cells in the experiment to assess their fragility under different osmotic pressures. The reagent bottles are used to specifically store these solutions to ensure their stability and availability during the experiment. The mixing assembly includes a limiting plate and several mixing test tubes. The mixing assembly provides a space in which red blood cells and solutions can be mixed. Attached Figure Description

[0017] Figure 1 This is a structural diagram of the present invention;

[0018] Figure 2 This is a structural diagram of the centrifuge assembly of this utility model;

[0019] Figure 3 This is a structural diagram of the hybrid component of this utility model;

[0020] Figure 4 This is a structural diagram of the pipetting assembly of this utility model;

[0021] Figure 5 This is a structural diagram of the clamping component of this utility model.

[0022] In all the accompanying drawings, the same reference numerals denote the same technical features, specifically: 1. Work stand; 2. Centrifuge assembly; 21. First support frame; 22. First inclined plate; 23. First servo motor; 24. Centrifuge tray; 241. Centrifuge tube; 3. Solution assembly; 31. Second support frame; 32. Solution box; 33. Reagent bottle; 4. Mixing assembly; 41. Third support frame; 42. Third inclined plate; 43. Second servo motor; 431. Pulley; 44. Limiting plate; 441. Rotating shaft; 45. Mixing tube; 5. Pipette assembly; 51. Fourth support frame; 52. Linear guide plate; 53. Ball screw; 54. Slider; 55. Mounting block; 56. Telescopic motor; 57. Pull-out block; 58. Negative pressure suction tube; 6. Clamping assembly; 61. Fifth support frame; 62. Fixing plate; 63. First telescopic rod; 64. Slide rod; 65. Triangular plate; 651. U-shaped plate; 66. Slide rail; 67. Fixed clamping rod; 68. Moving clamping rod; 69. Second telescopic rod; 7. Control assembly; 8. Spectrophotometer assembly; 81. Mounting box; 82. Spectrophotometer; 83. Cuvette. Detailed Implementation

[0023] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0024] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0025] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the present utility model and are not intended to limit the present utility model. Furthermore, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0027] like Figures 1-5 As shown, a colorimetric analyzer for erythrocyte osmotic fragility includes a work stand 1. A centrifugation component 2 for separating erythrocytes is fixedly installed on the upper surface of the work stand 1. A solution component 3 for holding solutions of different concentrations is fixedly installed on the upper surface of the work stand 1. A mixing component 4 for mixing erythrocytes and solutions is fixedly installed on the upper surface of the work stand 1. A pipetting component 5 for aspirating reagents is fixedly installed on one side of the mixing component 4. A sample-grabbing component 6 is provided on one side of the pipetting component 5. An electrically controlled control component 7 is fixedly installed on one side of the upper surface of the work stand 1. A spectrophotometric component 8 is fixedly installed on the upper surface of the work stand 1.

[0028] Solution assembly 3 includes a solution box 32, inside which are arranged several sets of reagent bottles 33. Mixing assembly 4 includes a limiting plate 44, inside which are installed several sets of mixing test tubes 45. Pipetting assembly 5 includes a fourth support frame 51, a linear guide plate 52, a ball screw 53, a slider 54, a mounting block 55, a telescopic motor 56, a pull block 57, and a negative pressure pipette 58. The fourth support frame 51 is fixedly installed on the upper surface of the workbench 1, and the linear guide plate 52 is fixedly installed between the fourth support frames 51. Rod 53 is fixedly installed in the middle of linear guide plate 52, slider 54 is slidably installed on the outside of ball screw 53, mounting block 55 is fixedly installed on the upper surface of slider 54, telescopic motor 56 is bolted to the surface of mounting block 55, one side of pull block 57 is bolted to the telescopic end of telescopic motor 56, the pull end of negative pressure pipette 58 is fixedly installed to pull block 57, negative pressure pipette 58 is slidably set on the upper surface of reagent bottle 33, and negative pressure pipette 58 draws the solution inside reagent bottle 33 and places it inside mixing test tube 45.

[0029] In this embodiment, the work stand 1 serves as the supporting structure for the entire analyzer, ensuring that all components can be stably and securely mounted on it, providing a stable working platform for the experiment. The centrifugation component 2 is used to centrifuge the red blood cell samples. Centrifugation is an important step in the red blood cell osmotic fragility experiment, as it helps to effectively separate red blood cells from the solution, thereby more accurately assessing the osmotic fragility of red blood cells. The solution component 3 includes a solution container 32 and several reagent bottles 33. The solution container 32 is used to store and manage solutions of different concentrations, which are used in the experiment to mix with red blood cells to assess their fragility under different osmotic pressures. The reagent bottles 33 are used for... To specifically store these solutions and ensure their stability and usability during experiments, the mixing assembly 4 includes a limiting disk 44 and several mixing tubes 45. The mixing assembly 4 provides a space for mixing red blood cells and solutions. The limiting disk 44 is used to fix the mixing tubes 45, ensuring their stability and positional accuracy during mixing. The mixing tubes 45 are the specific containers for mixing red blood cells and solutions. The fourth support frame 51 of the pipetting assembly 5 supports the linear guide plate 52, which is fixedly installed between the fourth support frames 51, providing a smooth and stable movement path for the slider 54. The ball screw 53 is installed... Mounted in the middle of the linear guide plate 52, it achieves precise position control through cooperation with the slider 54. The slider 54 is slidably mounted on the outside of the ball screw 53, and its movement is achieved by the rotation of the ball screw 53. A mounting block 55 is mounted on the slider 54 to support the telescopic motor 56. The mounting block 55 is fixedly mounted on the upper surface of the slider 54 to mount and support the telescopic motor 56. The telescopic motor 56 is bolted to the surface of the mounting block 55, and its telescopic movement drives the movement of the pull-out block 57. One side of the pull-out block 57 is bolted to the telescopic end of the telescopic motor 56, and the other side is fixedly mounted to the pull-out end of the negative pressure suction tube 58. The movement of the pipette 58 causes the negative pressure pipette 58 to move. The negative pressure pipette 58 is slidably set on the upper surface of the reagent bottle 33. It draws the solution through negative pressure. One end of the negative pressure pipette 58 is fixedly installed with the pull block 57, and the other end extends into the reagent bottle 33 or the mixing tube 45. The clamping component 6 is used to move the sample in the mixing tube 45 to a suitable position, so that the operator can move it into the spectrophotometer component 8. During the experiment, the pipette component 5 is driven by the telescopic motor 56 to move the negative pressure pipette 58 between the reagent bottle 33 and the mixing tube 45. When the negative pressure pipette 58 moves above the reagent bottle 33, it draws the required amount of solution through negative pressure.Then, it is moved above mixing tube 45 to release the solution into the tube. This process can be repeated to achieve precise addition and mixing of solutions of different concentrations. The absorbance or release of the solution is handled by a spectrophotometer, which measures the absorbance of the solution in mixing tube 45 to assess the osmotic fragility of red blood cells. These components integrate multiple functional modules such as centrifugation, solution management, mixing, pipetting, clamping, and spectrophotometry, simplifying the experimental procedure and providing strong support for red blood cell osmotic fragility experiments, reducing errors caused by human operation; the integration of multiple functional modules simplifies the experimental procedure; and improves the accuracy and efficiency of the experiment.

[0030] Specifically, the clamping assembly 6 includes a fifth support frame 61, a fixed plate 62, a first telescopic rod 63, a slide rod 64, a triangular plate 65, a slide rail 66, a fixed clamping rod 67, a movable clamping rod 68, and a second telescopic rod 69. The fifth support frame 61 is bolted to the upper surface of the workbench 1. The two ends of the fixed plate 62 are fixedly installed at the top of the fifth support frame 61. The first telescopic rod 63 is fixedly installed in the middle of the fixed plate 62. Two sets of slide rods 64 are fixedly installed at both ends of the fixed plate 62. The triangular plate 65 is fixedly installed at the telescopic end of the first telescopic rod 63. The two ends of the triangular plate 65 are sleeved with the slide rods 64. A U-shaped plate 651 is fixedly installed on the upper surface of the triangular plate 65. The slide rail 66 is bolted to the top wall of the U-shaped plate 651. The fixed clamping rod 67 is fixedly installed on the surface of one set of slide rails 66. The movable clamping rod 68 is slidably installed on the surface of another set of slide rails 66. The driving end of the movable clamping rod 68 is fixedly installed with the movable clamping rod 68.

[0031] In this embodiment, the fifth support frame 61 of the clamping assembly 6 is bolted to the upper surface of the workbench 1 to provide stable support for the entire clamping assembly 6. The two ends of the fixing plate 62 are fixedly installed on the top of the fifth support frame 61 as the mounting base for other components. The first telescopic rod 63 is fixedly installed in the middle of the fixing plate 62 to drive the triangular plate 65 to move up and down. Two sets of sliding rods 64 are fixedly installed at both ends of the fixing plate 62 and fitted with the triangular plate 65 to ensure the stability of the triangular plate 65 during movement. The triangular plate 65 is fixedly installed at the telescopic end of the first telescopic rod 63, and its two ends cooperate with the sliding rods 64. A U-shaped plate 651 is installed on the upper surface of the triangular plate 65. The surface provides an installation position for the slide rail 66, which is bolted to the top wall of the U-shaped plate 651. It provides a movement path for the fixed clamping rod 67 and the movable clamping rod 68. The fixed clamping rod 67 is fixedly installed on the surface of a set of slide rails 66 and is used to clamp one end of the sample. The movable clamping rod 68 is slidably installed on the surface of another set of slide rails 66. Its driving end is the second telescopic rod 69, which drives the position of the movable clamping rod 68. During the movement, the fixed clamping rod 67 and the movable clamping rod 68 remain clamped to ensure that the sample will not fall off. After reaching the target position, the driving end of the movable clamping rod 68 is activated again, driving the movable clamping rod 68 to move along the slide rail 66, separate from the fixed clamping rod 67, and release the sample.

[0032] Specifically, the hybrid assembly 4 also includes a third support frame 41, a third inclined plate 42, and a second servo motor 43. The third support frame 41 is fixedly installed on the upper surface of the workbench 1, the third inclined plate 42 is fixedly installed between the third support frames 41, and the second servo motor 43 is fixedly installed on the bottom wall of the third inclined plate 42. The drive end of the second servo motor 43 is provided with a pulley 431, and the bottom end of the limiting plate 44 is fixed with a rotating shaft 441, which is rotatably connected to the pulley 431.

[0033] In this embodiment, the third support frame 41 is fixedly installed on the upper surface of the workbench 1 to provide stable support for the entire mixing assembly 4. The third inclined plate 42 is fixedly installed between the third support frames 41 to form an inclined plane for mounting the second servo motor 43. The second servo motor 43 is fixedly installed on the bottom wall of the third inclined plate 42 as a drive source. It is connected to the rotating shaft 441 of the limiting plate 44 through the pulley 431 to realize the transmission of power. The pulley 431 is located at the drive end of the second servo motor 43 and is connected to the pulley 431 on the rotating shaft 441 through a belt to realize the transmission of power and the adjustment of the rotation speed. The limiting plate 4... 4 is used to fix the mixing test tube 45 to ensure its stability and positional accuracy during the mixing process. The bottom end of the limiting plate 44 is fixed with a rotating shaft 441, which is rotatably connected to the pulley 431. Thus, the limiting plate 44 is rotated by the drive of the second servo motor 43. When the second servo motor 43 is started, its drive end drives the pulley 431 to rotate, and the power is transmitted to the pulley 431 on the rotating shaft 441 through the belt, thereby driving the limiting plate 44 to rotate. Since the mixing test tube 45 is installed on the limiting plate 44, it rotates with the rotation of the limiting plate 44. The solution is fully mixed with the red blood cells under the action of centrifugal force.

[0034] Specifically, the solution assembly 3 also includes a second support frame 31, the bottom wall of which is fixedly installed on the upper surface of the work stand 1, and the bottom end of which is fixedly installed on the solution box 32, which is in an inclined state.

[0035] In this embodiment, the bottom wall of the second support frame 31 is fixedly installed on the upper surface of the work stand 1, which ensures the stability and positional accuracy of the solution component 3 in the entire experimental device. The solution box 32 is fixedly installed at the bottom of the second support frame 31 and is in an inclined state, which helps the solution to flow out and be distributed smoothly.

[0036] Specifically, the spectrophotometer assembly 8 includes a mounting box 81, a spectrophotometer 82, and cuvettes 83. The top surface of the mounting box 81 is a hinged cover. The spectrophotometer 82 is located inside the mounting box 81, and several sets of cuvettes 83 are installed in parallel inside the mounting box 81.

[0037] In this embodiment, after the clamping component 6 clamps the mixing test tube 45, the operator opens the mounting box 81 and adds solutions of different concentrations from the mixing test tube 45 into each cuvette 83. The solutions from the mixing test tube 45 are placed inside the cuvette 83. The top surface of the mounting box 81 is designed as a hinged cover, which is easy to open and close, facilitating the placement and removal of the cuvette 83 by the experimental operator, as well as the maintenance and calibration of the spectrophotometer 82. As a carrier for the spectrophotometer 82 and the cuvette 83, the mounting box 81 provides a stable and closed environment, which helps to reduce external light interference and maintain internal temperature stability. The absorbance of the solution is calculated by measuring the light intensity transmitted through each cuvette 83. Based on the relationship between absorbance and solution concentration, the concentration of the substance in the solution can be inferred.

[0038] Specifically, the centrifuge assembly 2 includes a first support frame 21, a first inclined plate 22, a first servo motor 23, and a centrifuge disc 24. The first support frame 21 is bolted to one side of the work stand 1. The first inclined plate 22 is arranged between the first support frames 21. The first servo motor 23 is fixedly installed on the bottom wall of the first inclined plate 22. The drive end of the first inclined plate 22 is fixedly connected to the middle of the centrifuge disc 24. Several sets of centrifuge tubes 241 are placed inside the centrifuge disc 24.

[0039] In this embodiment, the first support frame 21 is bolted to one side of the work stand 1, ensuring the stability and positional accuracy of the centrifuge assembly 2 in the entire experimental device. The first inclined plate 22 is set between the first support frames 21, forming an inclined plane. The first servo motor 23 is fixedly installed on the bottom wall of the first inclined plate 22. The first servo motor 23 outputs rotational power through its drive end to drive the centrifuge disc 24 to rotate. The centrifuge disc 24 is designed with several sets of centrifuge tubes 241 for holding blood to be centrifuged. The generated centrifugal force separates the red blood cells.

[0040] Specifically, the motors of the first servo motor 23, the second servo motor 43, the telescopic motor 56, the ball screw 53, and the first telescopic rod 63 are all electrically connected to the control component 7.

[0041] In this embodiment, the control component 7 can automatically adjust the operating status of each motor and driver through a preset program or algorithm to achieve a specific experimental purpose. In the red blood cell osmotic fragility experiment, the control component 7 can automatically adjust the rotation speed and time of the centrifugation component 2 and the mixing component 4, as well as the telescopic length of the telescopic motor 56 and the first telescopic rod 63, according to the experimental requirements.

[0042] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application. The above are merely preferred embodiments of this application. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of this application, and these improvements and modifications should also be considered within the protection scope of this application.

Claims

1. An osmotic fragility analyzer for red blood cells, comprising a workbench (1), characterized in that: The upper surface of the work stand (1) is fixedly equipped with a centrifugation component (2) for separating red blood cells, a solution component (3) for placing solutions of different concentrations, a mixing component (4) for mixing red blood cells and solutions, a pipetting component (5) for aspirating reagents, a clamping component (6) for picking up samples, an electrically controlled control component (7) for one side of the upper surface of the work stand (1), and a spectrophotometer component (8) for the upper surface of the work stand (1). The solution assembly (3) includes a solution box (32), inside which are arranged several sets of reagent bottles (33). The mixing assembly (4) includes a limiting plate (44), inside which are installed several sets of mixing test tubes (45). The pipetting assembly (5) includes a fourth support frame (51), a linear guide plate (52), a ball screw (53), a slider (54), a mounting block (55), a telescopic motor (56), a pull block (57), and a negative pressure pipette (58). The fourth support frame (51) is fixedly installed on the upper surface of the workbench (1), and the linear guide plate (52) is fixedly installed between the fourth support frames (51). The ball screw (53) is fixedly installed in the middle of the linear guide plate (52), the slider (54) is slidably installed on the outside of the ball screw (53), the mounting block (55) is fixedly installed on the upper surface of the slider (54), the telescopic motor (56) is bolted to the surface of the mounting block (55), one side of the pull block (57) is bolted to the telescopic end of the telescopic motor (56), the pull end of the negative pressure pipette (58) is fixedly installed to the pull block (57), the negative pressure pipette (58) is slidably set on the upper surface of the reagent bottle (33), and the negative pressure pipette (58) draws the solution inside the reagent bottle (33) and places it inside the mixing tube (45).

2. The osmotic fragility colorimetric assay analyzer for red blood cells according to claim 1, characterized in that: The clamping assembly (6) includes a fifth support frame (61), a fixed plate (62), a first telescopic rod (63), a slide rod (64), a triangular plate (65), a slide rail (66), a fixed clamping rod (67), a movable clamping rod (68), and a second telescopic rod (69). The fifth support frame (61) is bolted to the upper surface of the workbench (1). The two ends of the fixed plate (62) are fixedly installed at the top of the fifth support frame (61). The first telescopic rod (63) is fixedly installed in the middle of the fixed plate (62). The two sets of slide rods (64) are fixedly installed on the fixed plate (69). 2) At both ends, the triangular plate (65) is fixedly installed at the telescopic end of the first telescopic rod (63). The two ends of the triangular plate (65) are sleeved with the slide rod (64). A U-shaped plate (651) is fixedly installed on the upper surface of the triangular plate (65). The slide rail (66) is bolted to the top wall of the U-shaped plate (651). The fixed clamping rod (67) is fixedly installed on the surface of a set of slide rails (66). The movable clamping rod (68) is slidably installed on the surface of another set of slide rails (66). The driving end of the movable clamping rod (68) is fixedly installed with the movable clamping rod (68).

3. The osmotic fragility colorimetric assay analyzer of claim 1, wherein: The hybrid assembly (4) also includes a third support frame (41), a third inclined plate (42), and a second servo motor (43). The third support frame (41) is fixedly installed on the upper surface of the workbench (1). The third inclined plate (42) is fixedly installed between the third support frames (41). The second servo motor (43) is fixedly installed on the bottom wall of the third inclined plate (42). The drive end of the second servo motor (43) is provided with a pulley (431). The bottom end of the limiting plate (44) is fixed with a rotating shaft (441). The rotating shaft (441) is rotatably connected to the pulley (431).

4. The osmotic fragility colorimetric assay analyzer of claim 1, wherein: The solution assembly (3) also includes a second support frame (31), the bottom wall of which is fixedly installed on the upper surface of the work stand (1), and the bottom end of which is fixedly installed on the solution box (32), which is in an inclined state.

5. The osmotic fragility colorimetric assay analyzer of claim 1, wherein: The spectrophotometric assembly (8) includes a mounting box (81), a spectrophotometer (82), and cuvettes (83). The top surface of the mounting box (81) is a hinged cover. The spectrophotometer (82) is located inside the mounting box (81). Several sets of cuvettes (83) are installed in parallel inside the mounting box (81).

6. The osmotic fragility colorimetric assay analyzer of claim 1, wherein: The centrifuge assembly (2) includes a first support frame (21), a first inclined plate (22), a first servo motor (23), and a centrifuge tray (24). The first support frame (21) is bolted to one side of the work stand (1). The first inclined plate (22) is arranged between the first support frames (21). The first servo motor (23) is fixedly installed on the bottom wall of the first inclined plate (22). The driving end of the first inclined plate (22) is fixedly connected to the middle of the centrifuge tray (24). Several sets of centrifuge tubes (241) are placed inside the centrifuge tray (24).

7. The osmotic fragility colorimetric assay analyzer of claim 6, wherein: The first servo motor (23), the second servo motor (43), the telescopic motor (56), the motor of the ball screw (53) and the first telescopic rod (63) are electrically connected with the control assembly (7).

Images