Culture dish and sample culture system

Abstract

The utility model relates to a culture dish and a sample culture system, the culture dish comprises a ventilation assembly, an upper dish body assembly and a lower dish body assembly; the upper dish body assembly comprises a dish cover and a dish body and is detachably connected with the dish body through threads. The lower dish body assembly comprises a dish bottom and a transparent piece, an observation window is formed in the bottom wall of the dish bottom, the transparent piece covers the observation window, the dish bottom covers the second end of the dish body through a thread, and a containing space is defined by the upper dish body assembly and the lower dish body assembly; the ventilation assembly comprises a first pipe body and a second pipe body which are communicated with the containing space. The air pressure and the gas composition in the culture dish are controllable, and the change process of a sample in the culture dish can be observed and recorded in real time. The culture dish is small in size, gas types and gas pressure in the culture dish can be accurately controlled by using a small amount of gas, gas consumption is reduced, and cost is reduced.

Description

Technical Field

[0001] This utility model relates to the field of laboratory equipment technology, and in particular to a petri dish and a sample culture system. Background Technology

[0002] Real-time observation during cell and organoid culture allows for the recording of the effects of the culture environment, such as pressure, gas composition, growth factors, and drugs, on cell and organoid growth.

[0003] Although existing technologies exist for culture dishes that can adjust the internal gas pressure, they cannot allow for real-time observation of the samples within the culture dish. Utility Model Content

[0004] One objective of this invention is to provide a petri dish that at least solves one of the aforementioned technical problems.

[0005] To achieve the above objectives, the first aspect of this utility model provides a petri dish, including an aeration assembly, an upper dish assembly, and a lower dish assembly;

[0006] The upper dish assembly includes a dish lid and a dish body. The dish body is a hollow structure that extends through both ends. The dish lid is placed on the first end of the dish body and is detachably connected to the dish body by threads. The dish lid and the dish body are sealed together.

[0007] The lower dish assembly includes a dish bottom and a transparent sheet. The bottom wall of the dish bottom has an observation window. The transparent sheet is placed inside the dish bottom and covers the observation window. The dish bottom is threaded onto the second end of the dish body, and the second end of the dish body can seal against the transparent sheet.

[0008] The upper vessel assembly and the lower vessel assembly enclose a receiving space;

[0009] The ventilation assembly includes a first tube and a second tube, both of which are connected to the upper vessel assembly and communicate with the accommodating space.

[0010] Optionally, the lid includes a cover plate and a first annular wall, the first annular wall being connected to one side of the cover plate, and the first annular wall having an internal thread;

[0011] The bottom of the dish includes an annular base plate and a second annular wall, the second annular wall being connected to one side of the annular base plate and having internal threads; the inner ring of the annular base plate forms the observation window;

[0012] The vessel body includes a third annular wall with an external thread. The internal thread of the first annular wall is connected to the external thread of the third annular wall, and the internal thread of the second annular wall is connected to the external thread of the third annular wall.

[0013] Optionally, the petri dish further includes a first sealing ring, which is disposed between the dish lid and the dish body, and abuts against the dish lid and the dish body respectively, and / or

[0014] The petri dish also includes a second sealing ring, which is disposed between the bottom of the dish and the body of the dish, and abuts against the bottom of the dish and the body of the dish respectively.

[0015] Optionally, a first annular groove is formed on the end face of the first end of the vessel body, and a first sealing ring is disposed in the first annular groove, the first sealing ring abutting against the groove wall of the first annular groove and the inner side of the cover plate; and / or

[0016] The second end face of the vessel body has a second annular groove, and the second sealing ring is disposed in the second annular groove. The second sealing ring abuts against the groove wall of the second annular groove and the inner side of the transparent sheet.

[0017] Optionally, the vessel body further includes a convex ring, which is disposed on the outside of the third annular wall and extends circumferentially along the third annular wall, and the convex ring is located between the first annular wall and the second annular wall.

[0018] Optionally, the cover plate has a first hole and a second hole extending through its thickness direction. One end of the first tube is connected to the outside of the cover plate and is directly opposite to the first hole. One end of the second tube is connected to the outside of the cover plate and is directly opposite to the second hole.

[0019] Optionally, an annular positioning groove is provided on the inner side of the annular base plate, and the transparent sheet is placed in the annular positioning groove.

[0020] Another objective of this invention is to provide a sample culture system to at least solve one of the aforementioned technical problems.

[0021] To achieve this objective, the second aspect of this utility model adopts the following technical solution:

[0022] A sample culture system includes an inlet pipe, an outlet pipe, a gas source, a first valve, a pressure detection and control component, and a culture dish. One end of the inlet pipe is connected to the gas source, and the other end of the inlet pipe and the outlet pipe are respectively connected to the ventilation component. The pressure detection and control component is connected to the inlet pipe, and the first valve is connected to the outlet pipe.

[0023] Optionally, the pressure detection and control component includes a pressure controller connected to the intake pipe.

[0024] Optionally, the sample culture system includes one culture dish or at least two culture dishes. When there are two culture dishes, the two culture dishes are connected in series or in parallel. When there are more than two culture dishes, multiple culture dishes are connected in series and / or in parallel.

[0025] As can be seen from the above, the technical solution provided by this utility model involves placing a transparent sheet inside the bottom of the petri dish and covering the observation window. The sample to be observed is then placed or smeared onto the transparent sheet. The bottom of the dish is then threaded to the second end of the dish body to achieve a sealed connection. Subsequently, culture medium is poured into the containing space through the opening at the top of the dish body. Finally, the dish lid is connected to the first end of the dish body. Connecting a gas source to the aeration assembly allows gas to be introduced into the containing space. By changing the type of gas in the gas source, the type of gas in the petri dish can be precisely controlled. Controlling the aeration rate also controls the gas pressure within the containing space, allowing cells and organoids to grow under specific pressure. Using a fluorescence microscope, such as a wide-field microscope, confocal fluorescence microscope, or two-photon microscope, high-resolution imaging of cells and organoids can be achieved through the transparent sheet, enabling real-time observation and recording of changes in the samples. Furthermore, the petri dish is small in size, requiring only a small amount of gas to achieve precise control of the gas type and pressure, reducing gas consumption and costs.

[0026] Because the second end of the dish body can seal against the transparent sheet, the culture medium will not leak between the transparent sheet and the dish lid. The transparent sheet can be installed simply by placing it inside the bottom of the dish; its installation and removal are simple and easy to replace.

[0027] Since the second end of the dish body is sealed to the transparent sheet and the bottom of the dish body after the dish lid, dish body and dish bottom are connected, there will be no leakage of liquid or air, ensuring that the experiment can proceed smoothly. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the structure of the petri dish provided in an embodiment of this utility model;

[0029] Figure 2 This is a schematic diagram of the petri dish from another perspective provided in an embodiment of this utility model;

[0030] Figure 3 This is a cross-sectional view of the petri dish provided in an embodiment of this utility model;

[0031] Figure 4This is an exploded view of the petri dish provided in an embodiment of this utility model;

[0032] Figure 5 This is a schematic diagram of the structure of the dish lid provided in an embodiment of this utility model;

[0033] Figure 6 This is a schematic diagram of the structure of the dish bottom provided in an embodiment of this utility model;

[0034] Figure 7 This is a schematic diagram of the first sample culture system (with a culture dish) provided in this embodiment of the present invention;

[0035] Figure 8 This is a schematic diagram of the second sample culture system (with two culture dishes connected in series) provided in this embodiment of the present invention;

[0036] Figure 9 This is a schematic diagram of the third sample culture system (with two parallel culture dishes) provided in this embodiment of the present invention;

[0037] Figure 10 This is a schematic diagram of the fourth sample culture system (with two parallel culture dishes) provided in this embodiment of the present invention;

[0038] Figure 11 This is a schematic diagram of the fifth sample culture system provided in this embodiment of the present invention (three systems are provided, of which two culture dishes are connected in series and one culture dish is connected in parallel);

[0039] Figure 12 This is a schematic diagram of the sixth sample culture system provided in this embodiment of the present invention (three systems are provided, of which two culture dishes are connected in series and one culture dish is connected in parallel);

[0040] Figure 13 This is a schematic diagram of the seventh sample culture system (with three culture dishes connected in series) provided in this embodiment of the present invention;

[0041] Figure 14 This is a schematic diagram of the eighth sample culture system (with three parallel culture dishes) provided in this embodiment of the present invention.

[0042] In the picture:

[0043] 1. Dish lid; 11. Lid plate; 111. First hole; 112. Second hole; 12. First annular wall;

[0044] 2. Dish body; 21. First annular groove; 22. Second annular groove; 23. Third annular wall; 24. Protruding ring;

[0045] 3. Lower dish assembly; 31. Dish bottom; 311. Observation window; 312. Annular base plate; 3121. Annular positioning groove; 313. Second annular wall; 32. Transparent sheet;

[0046] 4. Accommodation space; 5. First sealing ring; 6. Second sealing ring;

[0047] 7. Ventilation assembly; 71. First tube body; 72. Second tube body;

[0048] 10. Petri dish; 20. Air inlet pipe; 30. Air outlet pipe; 40. Air source; 50. First valve; 60. Pressure controller. Detailed Implementation

[0049] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining this utility model and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts related to this utility model are shown in the accompanying drawings, not all of them.

[0050] This utility model defines certain directional terms. Unless otherwise stated, the directional terms used, such as "up", "down", "left", "right", "inner", and "outer", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of this utility model.

[0051] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0052] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0053] This embodiment provides a culture dish 10 for culturing biological samples such as live cells, tissues, or organoids. For example, the live cells can be adherent live cells or removed biological tissues, etc. The types of biological samples are not limited to these, so that the samples in the culture dish 10 can be observed in real time.

[0054] like Figures 1-4 As shown, the culture dish 10 provided in this embodiment includes an aeration assembly 7, an upper dish assembly, and a lower dish assembly 3.

[0055] The upper dish assembly includes a dish lid 1 and a dish body 2. The dish body 2 is a hollow structure with both ends open. The dish lid 1 is placed on the first end of the dish body 2 and is detachably connected to the dish body 2 by threads. The dish lid 1 and the dish body 2 are sealed together.

[0056] The lower dish assembly 3 includes a dish bottom 31 and a transparent sheet 32. An observation window 311 is provided on the bottom wall of the dish bottom 31. The transparent sheet 32 ​​is placed inside the dish bottom 31 and covers the observation window 311. The dish bottom 31 is threaded onto the second end of the dish body 2, and the second end of the dish body 2 can seal against the transparent sheet 32. Optionally, the transparent sheet 32 ​​is made of glass. Glass has high transparency and high image quality. The material of the transparent sheet 32 ​​is not limited to glass and can also be other transparent materials.

[0057] The upper and lower vessel components 3 form a receiving space 4. The ventilation component 7 includes a first tube 71 and a second tube 72. Both the first tube 71 and the second tube 72 are connected to the upper vessel component and communicate with the receiving space 4.

[0058] When using the culture dish 10 provided in this embodiment, a transparent sheet 32 ​​is placed inside the bottom 31 of the dish and covers the observation window 311. The sample to be observed is placed or smeared on the transparent sheet 32, or biological samples such as cells or organoids have been cultured on the transparent sheet 32 ​​in advance. Then, the bottom 31 of the dish is threaded to the second end of the dish body 2 to achieve a sealed connection between the bottom 31 and the dish body 2. Subsequently, culture medium is poured into the containing space 4 through the opening at the upper end (first end) of the dish body 2. Finally, the dish lid 1 is connected to the first end of the dish body 2. Connecting the gas source 40 to the aeration assembly 7 allows gas to be introduced into the containing space 4. By changing the type of gas in the gas source 40, the type of gas in the culture dish 10 can be precisely controlled. By controlling the ventilation rate, the gas pressure within the containment space 4 can also be controlled, allowing samples such as cells and organoids to grow under specific pressure. Using fluorescence microscopy, such as a wide-field microscope, confocal fluorescence microscope, or two-photon microscope, high-resolution imaging of samples such as cells and organoids can be achieved through the transparent slide 32, enabling real-time observation and recording of changes in the samples. Furthermore, the small volume of the culture dish 10 allows for precise control of the gas type and pressure using only a small amount of gas, reducing gas consumption and lowering costs.

[0059] Because the second end of the dish body 2 can be sealed against the transparent sheet 32, the culture medium will not leak between the transparent sheet 32 ​​and the dish cap 1. The transparent sheet 32 ​​can be installed simply by placing it inside the dish bottom 31. The transparent sheet 32 ​​is easy to install, remove, and replace.

[0060] Since the lid 1, body 2, and bottom 31 are connected, the second end of body 2 is sealed to the transparent sheet 32, and the bottom 31 is sealed to body 2. Therefore, there will be no leakage of liquid or air, ensuring the smooth progress of the experiment.

[0061] Optionally, to ensure imaging quality, the transparent sheet 32 ​​can be used only once, while the lid 1, body 2, and bottom 31 can be sterilized and reused, reducing consumable costs. Optionally, the lid 1, body 2, and bottom 31 are made of stainless steel, which maintains structural stability and safety under specific pressure.

[0062] like Figure 3 , Figure 4 and Figure 5 As shown, the lid 1 includes a lid plate 11 and a first annular wall 12. The first annular wall 12 is connected to one side of the lid plate 11 and has internal threads. Optionally, the lid plate 11 is a circular plate and the first annular wall 12 is a ring. The lid plate 11 and the first annular wall 12 can be integrally formed.

[0063] The dish bottom 31 includes an annular base plate 312 and a second annular wall 313. The second annular wall 313 is connected to one side of the annular base plate 312 and has internal threads. The inner ring of the annular base plate 312 forms an observation window 311. The annular base plate 312 and the second annular wall 313 can be integrally formed.

[0064] The vessel body 2 includes a third annular wall 23, on which an external thread is provided. The internal thread of the first annular wall 12 is connected to the external thread of the third annular wall 23, and the internal thread of the second annular wall 313 is connected to the external thread of the third annular wall 23.

[0065] The lid 1, body 2, and bottom 31 have a simple structure, are easy to process and connect, and are easy to load and unload.

[0066] like Figure 3 and Figure 4 As shown, the petri dish 10 may also include a first sealing ring 5, which is disposed between the lid 1 and the body 2. The first sealing ring 5 abuts against the lid 1 and the body 2 respectively, thereby achieving a seal between the lid 1 and the body 2.

[0067] The petri dish 10 may also include a second sealing ring 6, which is disposed between the bottom 31 and the body 2 of the dish. The second sealing ring 6 abuts against the bottom 31 and the body 2 of the dish respectively, thereby achieving a seal between the bottom 31 and the body 2 of the dish.

[0068] A first annular groove 21 is formed on the end face of the first end of the vessel body 2. A first sealing ring 5 is disposed in the first annular groove 21. The first sealing ring 5 abuts against the groove wall of the first annular groove 21 and the inner side of the cover plate 11. It can be understood that the inner side of the cover plate 11 refers to the side of the cover plate 11 facing the receiving space 4. For example, the first sealing ring 5 is an O-ring.

[0069] like Figure 3 As shown, a second annular groove 22 is formed on the end face of the second end of the vessel body 2. A second sealing ring 6 is disposed in the second annular groove 22. The second sealing ring 6 abuts against the groove wall of the second annular groove 22 and the inner side of the transparent sheet 32. It can be understood that the inner side of the transparent sheet 32 ​​refers to the side of the transparent sheet 32 ​​facing the receiving space 4. For example, the second sealing ring 6 is an O-ring.

[0070] In this embodiment, the first sealing ring 5 protrudes from the first annular sealing groove. When the end face of the first end of the dish body 2 abuts against the inner side of the cover plate 11, the first sealing ring 5 seals against the inner side of the cover plate 11. The second sealing ring 6 protrudes from the second annular groove 22. When the end face of the second end of the dish body 2 abuts against the inner side of the annular base plate 312, the second sealing ring 6 seals against the inner side of the annular base plate 312. At this time, along the axial direction of the dish body 2, the size of the accommodating space 4 is basically the same as the height of the dish body 2. Therefore, the size of the accommodating space 4 is the size of the internal space of the dish body 2.

[0071] To facilitate the assembly and disassembly of the culture dish 10, the dish body 2 may optionally include a raised ring 24. The raised ring 24 is disposed on the outer side of the third annular wall 23 and extends circumferentially along the third annular wall 23. After the dish lid 1, dish body 2, and dish bottom 31 are connected, the raised ring 24 is located between the first annular wall 12 and the second annular wall 313. For example, the raised ring 24 is connected to the middle portion of the third annular wall 23 in the axial direction. The operator applies force to the raised ring 24 by contacting it, thus rotating the dish body 2 to connect it to the dish lid 1 or the dish bottom 31. The raised ring 24 and the third annular wall 23 can be integrally formed.

[0072] like Figure 3 and Figure 5 As shown, the cover plate 11 has a first hole 111 and a second hole 112 extending through its thickness. One end of the first tube 71 is connected to the outside of the cover plate 11 and is directly opposite to the first hole 111. One end of the second tube 72 is connected to the outside of the cover plate 11 and is directly opposite to the second hole 112. One of the first tube 71 and the second tube 72 allows air to enter, while the other allows air to exit, thus circulating the gas in the culture dish 10 and ensuring that the gas composition and concentration in the culture dish 10 remain stable. The cover plate 11, the first tube 71, and the second tube 72 can be integrally formed.

[0073] During the experiment, the cells are cultured on a transparent slide 32, and the culture dish 10 can be placed on an inverted fluorescence microscope, such as a regular wide-field microscope, a confocal fluorescence microscope, a two-photon microscope, etc. High-resolution imaging of the cells can be achieved through the transparent slide 32.

[0074] In other alternative embodiments, the first and second holes may not be located on the lid 1, but rather on the body 2 of the dish, extending radially along the body 2. However, when the first and second holes are located on the body 2, the culture medium in the containing space 4 cannot exceed the first and second holes; otherwise, leakage and the inability to inject gas into the containing space 4 will occur, resulting in low utilization of the containing space 4. Preferably, the first hole 111 and the second hole 112 are located on the lid 1, so that the first hole 111 and the second hole 112 do not affect the capacity of the culture medium in the containing space 4, thereby improving the utilization of the containing space 4.

[0075] like Figure 6 As shown, an annular positioning groove 3121 is provided on the inner side of the annular base plate 312, and the transparent sheet 32 ​​is placed in the annular positioning groove 3121. The annular positioning groove 3121 can position the transparent sheet 32 ​​and prevent the second sealing ring 6 from failing to abut against the transparent sheet 32 ​​if the transparent sheet 32 ​​is not placed in the correct position.

[0076] like Figure 7 As shown, the sample culture system includes an inlet pipe 20, an outlet pipe 30, a gas source 40, a first valve 50, a pressure detection and control component, and a culture dish 10. One end of the inlet pipe 20 is connected to the gas source 40, and the other end of the inlet pipe 20 is connected to the first tube 71. The outlet pipe 30 is connected to the second tube 72. The pressure detection and control component is connected to the inlet pipe 20, and the first valve 50 is connected to the outlet pipe 30.

[0077] The gas source 40 may include a gas cylinder and gas stored in the gas cylinder. The gas source 40 may be a single gas or a mixture of multiple gases. Optionally, the inlet pipe 20 and the outlet pipe 30 may be silicone tubing.

[0078] For example, the other end of the intake pipe 20 is connected to the first pipe body 71, and the exhaust pipe 30 is connected to the second pipe body 72.

[0079] When samples need to be cultured under atmospheric pressure and specific gas conditions, the first valve 50 is opened, and the gas source 40 continuously introduces gas into the culture dish 10 through the gas inlet pipe 20. The gas in the culture dish 10 is continuously discharged through the gas outlet pipe 30, ensuring that the gas pressure, gas composition and concentration in the culture dish 10 remain stable.

[0080] When samples need to be cultured in a specific gas at a preset pressure, the first valve 50 is opened to allow gas to pass through the culture dish 10, purging the air from the dish. The first valve 50 is then closed. When the pressure detection and control component detects that the pressure in the inlet pipe 20 has reached a preset value, the gas flow is stopped, and the inlet pipe 20 and culture dish 10 are connected. At this time, the preset pressure is maintained in the culture dish 10. After a preset time, the gas in the culture dish 10 is consumed, and the gas in the culture dish 10 can be replaced to maintain a stable gas composition and concentration. Specifically, the first valve 50 is opened to allow gas to pass through the culture dish 10, purging the existing gas. The first valve 50 is then closed, and gas is continued to pass through until the pressure in the culture dish 10 reaches the preset value. Gas flow is then stopped, maintaining the preset pressure in the culture dish 10.

[0081] While observing and recording samples using a fluorescence microscope, ventilation, gas exchange, and pressure maintenance can also be performed to ensure that the gas composition and pressure in the culture dish 10 are maintained at preset values.

[0082] The first valve 50 can be a manual valve or an electric valve.

[0083] Optionally, the pressure detection and control component includes a pressure controller 60, which is connected to the intake pipe 20. The pressure controller 60 can detect the pressure and maintain the pressure in the intake pipe 20 at a preset value. The pressure controller 60 is prior art, and its specific structure will not be described in detail here.

[0084] In other alternative embodiments, the pressure detection and control component may include a pressure gauge and a second valve, wherein the opening and closing of the second valve is determined by the pressure detected by the pressure gauge. The second valve may be a manual valve or an electric valve.

[0085] When the second valve can be a manual valve, the operator can manually control the opening and closing of the second valve based on the pressure gauge reading.

[0086] When the second valve is an electrically operated valve, the pressure gauge can be an electronic pressure gauge, and the pressure detection and control component can also include a controller. Both the pressure gauge and the second valve are connected to the controller. The controller controls whether the second valve opens based on the pressure detected by the pressure gauge. In this case, the first valve 50 can be a manual valve or an electrically operated valve. If the first valve 50 is a manual valve, the operator controls its opening and closing according to whether venting is required. If the first valve 50 is an electrically operated valve, it can also be connected to the controller. The controller can control the first valve 50 to open at predetermined intervals. After the first valve 50 opens, it controls the second valve to open. The first valve 50 closes after a preset time to purge the gas from the culture dish. After receiving the pressure signal from the pressure gauge and determining that the pressure signal has reached a preset value, the controller controls the second valve to close, thereby maintaining the preset pressure conditions in the culture dish.

[0087] The controller is an existing technology and can be a centralized or distributed controller. For example, the controller can be a single microcontroller or a distributed system of multiple microcontrollers. The microcontroller can run a control program to control the aforementioned components to achieve their functions.

[0088] like Figure 7 As shown, the sample culture system may include a culture dish 10. Figures 8-12 As shown, the sample culture system may include at least two culture dishes 10, such as Figures 8-10 As shown, when there are two culture dishes 10, the two culture dishes 10 are connected in series or in parallel; as Figures 11-14 As shown, when the number of culture dishes 10 is greater than two, multiple culture dishes 10 are connected in series and / or in parallel.

[0089] Multiple culture dishes 10 are cultured in series and / or in parallel to achieve batch experiments with stable experimental conditions and high reproducibility, thus greatly saving experimental time.

[0090] Although the present invention has been described in detail above with general descriptions, specific embodiments, and experiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A petri dish, characterized in that, It includes a ventilation assembly (7), an upper dish assembly, and a lower dish assembly (3); The upper dish assembly includes a dish lid (1) and a dish body (2). The dish body (2) is a hollow structure with both ends through. The dish lid (1) is placed on the first end of the dish body (2) and is detachably connected to the dish body (2) by threads. The dish lid (1) and the dish body (2) are sealed together. The lower dish assembly (3) includes a dish bottom (31) and a transparent sheet (32). The bottom wall of the dish bottom (31) is provided with an observation window (311). The transparent sheet (32) is placed inside the dish bottom (31) and covers the observation window (311). The dish bottom (31) is threaded onto the second end of the dish body (2), and the second end of the dish body (2) can seal against the transparent sheet (32). The upper vessel assembly and the lower vessel assembly (3) form a receiving space (4); The ventilation assembly (7) includes a first tube (71) and a second tube (72), both of which are connected to the upper body assembly and communicate with the receiving space (4).

2. The petri dish according to claim 1, characterized in that, The lid (1) includes a cover plate (11) and a first annular wall (12), the first annular wall (12) being connected to one side of the cover plate (11), and the first annular wall (12) having an internal thread; The bottom of the dish (31) includes an annular bottom plate (312) and a second annular wall (313), the second annular wall (313) being connected to one side of the annular bottom plate (312), and the second annular wall (313) having an internal thread; the inner ring of the annular bottom plate (312) forms the observation window (311); The vessel body (2) includes a third annular wall (23), on which an external thread is provided. The internal thread of the first annular wall (12) is connected to the external thread of the third annular wall (23), and the internal thread of the second annular wall (313) is connected to the external thread of the third annular wall (23).

3. The petri dish according to claim 2, characterized in that, The petri dish further includes a first sealing ring (5), which is disposed between the lid (1) and the body (2). The first sealing ring (5) abuts against the lid (1) and the body (2) respectively, and / or The culture dish also includes a second sealing ring (6), which is disposed between the bottom (31) and the body (2) of the dish, and the second sealing ring (6) abuts against the bottom (31) and the body (2) of the dish respectively.

4. The petri dish according to claim 3, characterized in that, A first annular groove (21) is formed on the end face of the first end of the vessel body (2), and a first sealing ring (5) is disposed in the first annular groove (21). The first sealing ring (5) abuts against the groove wall of the first annular groove (21) and the inner side of the cover plate (11); and / or The second end face of the vessel body (2) is provided with a second annular groove (22), and the second sealing ring (6) is disposed in the second annular groove (22). The second sealing ring (6) abuts against the groove wall of the second annular groove (22) and the inner side of the transparent sheet (32).

5. The petri dish according to any one of claims 2-4, characterized in that, The vessel body (2) also includes a convex ring (24), which is disposed on the outside of the third annular wall (23) and extends circumferentially along the third annular wall (23). The convex ring (24) is located between the first annular wall (12) and the second annular wall (313).

6. The petri dish according to claim 2, characterized in that, The cover plate (11) has a first hole (111) and a second hole (112) extending through its thickness direction. One end of the first tube (71) is connected to the outside of the cover plate (11) and is directly opposite to the first hole (111). One end of the second tube (72) is connected to the outside of the cover plate (11) and is directly opposite to the second hole (112).

7. The petri dish according to claim 2, characterized in that, The inner side of the annular base plate (312) is provided with an annular positioning groove (3121), and the transparent sheet (32) is placed in the annular positioning groove (3121).

8. A sample culture system, characterized in that, The system includes an inlet pipe (20), an outlet pipe (30), an air source (40), a first valve (50), a pressure detection and control component, and a petri dish (10) as described in any one of claims 1-7. One end of the inlet pipe (20) is connected to the air source (40), and the other end of the inlet pipe (20) and the outlet pipe (30) are respectively connected to the ventilation component (7). The pressure detection and control component is connected to the inlet pipe (20), and the first valve (50) is connected to the outlet pipe (30).

9. The sample culture system according to claim 8, characterized in that, The pressure detection and control component includes a pressure controller (60) connected to the intake pipe (20).

10. The sample culture system according to claim 8, characterized in that, The sample culture system includes one culture dish (10) or at least two culture dishes (10). When there are two culture dishes (10), the two culture dishes (10) are connected in series or in parallel. When there are more than two culture dishes (10), multiple culture dishes (10) are connected in series and / or in parallel.

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