Flow cytometry device

Abstract

The utility model relates to the technical field of medical instruments, in particular to a flow cytometry device. The sheath fluid flow is conveyed into the flowing chamber through the conveying channel, meanwhile, the sample flow is conveyed into the flowing chamber through the sample injection needle and mixed with the sheath fluid flow to form mixed liquid, and cells in a sample are wrapped in the center of the sheath fluid through hydrodynamic focusing. The converged sheath fluid flow and the sample flow sequentially pass through the irradiation chamber, are irradiated by laser and then are discharged from the discharge joint on the discharge pipe, and an irradiation chamber and flow chamber integrated structure is arranged on the flow type pool component, so that manual adjustment operation for centering during later assembly is avoided; meanwhile, the problem that the liquid path effect is affected due to the fact that the irradiation chamber and the flow chamber are not centered or the relative position deviation of the irradiation chamber and the flow chamber in the using process is effectively solved.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and specifically to a flow cytometry device. Background Technology

[0002] A flow cytometer is a device for automated analysis and sorting of cells. It can rapidly measure, store, and display a range of important biophysical and biochemical characteristic parameters of dispersed cells suspended in a liquid, and can sort specific cell subpopulations from a pre-selected parameter range. It mainly consists of a flow chamber and fluid flow system, a laser source and optical system, a phototube and detection system, and a computer and analysis system.

[0003] In existing flow cytometers, the irradiation chamber and the flow chamber are two separate parts. During installation, it is very easy for the irradiation chamber and the flow chamber to be misaligned, or for their relative positions to shift during use, affecting the liquid path effect. Therefore, it is necessary to frequently manually align and adjust the two, which not only affects the efficiency of use, but also the accuracy of use. Utility Model Content

[0004] The technical problem to be solved by this utility model is to provide a flow cytometer device with an integrated structure of irradiation chamber and flow chamber, which eliminates the need for manual adjustment of centering during later assembly and eliminates the impact of relative positional misalignment of the irradiation chamber and flow chamber on the liquid path effect.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0006] A flow cytometry device, comprising:

[0007] A base on which a flow cell component is provided, a flow chamber is provided at the lower part of the flow cell component, and an irradiation chamber is provided at the top of the flow cell component, the flow chamber being connected to the irradiation chamber;

[0008] The base is provided with a material conveying channel, which is connected to the flow chamber and is used to convey the sheath fluid flow into the flow chamber;

[0009] A sample injection assembly is disposed below the base. The sample injection assembly includes a mounting base and a sample injection needle. The sample injection needle is connected to the base through the mounting base. The discharge end of the sample injection needle passes through the material delivery channel and is located in the flow chamber. The sample injection needle is used to deliver the sample flow into the flow chamber and mix it with the sheath fluid flow to form a mixture.

[0010] The discharge assembly is connected to the flow cell component and is connected to the irradiation chamber. The discharge assembly is used to discharge the irradiated mixture from the irradiation chamber as waste liquid.

[0011] In one embodiment of this utility model, the flow cell component includes a column, a shoulder is provided on the column, a fixing block is provided on the shoulder, the fixing block, the shoulder and the column are integrally formed, the irradiation chamber is disposed in the fixing block, and a cover plate is provided on the base. The cover plate is used to fix the column to the base, and the shoulder is engaged with the cover plate.

[0012] In one embodiment of this utility model, the cross-section of the shoulder platform is circular or polygonal, and the cover plate is provided with a positioning hole that matches the shoulder platform, with the shoulder platform passing through the positioning hole.

[0013] In one embodiment of the present invention, at least one limiting hole is provided on the cover plate, and a fixing pin is provided on the bottom of the cover plate, the fixing pin being provided on both sides of the positioning hole.

[0014] In one embodiment of this utility model, the column has a conical cylindrical structure, the base is provided with a conical hole that matches the column, the conical hole is provided with a through hole that communicates with the material conveying channel, and the column is sealed to the through hole.

[0015] In one embodiment of this utility model, the discharge assembly includes a discharge rack, which is disposed on the cover plate. A pressure block is disposed on the discharge rack, which is connected to the flow cell component. A discharge pipe is disposed on the pressure block, which is connected to the irradiation chamber. A discharge connector is disposed on the discharge pipe.

[0016] In one embodiment of this utility model, the feed end of the material conveying channel is provided with an internal threaded hole, the internal threaded hole is connected to the material conveying connector, and a sealing ring is provided between the material conveying connector and the base.

[0017] In one embodiment of this utility model, the injection needle is mounted on a fixed base, the fixed base has a conical columnar structure, the lower part of the base has a fixing hole that matches the fixed base, the fixed base is sealed to the fixing hole, and the fixed base is connected to the base by a fixing nut.

[0018] In one embodiment of the present invention, the mounting base includes a pipe connector, a pressure ring is provided on the pipe connector, the pressure ring is made of rubber material, an inlet pipe is passed through the pressure ring, an inlet hole is provided on the fixing base, and the pipe connector is inserted into the inlet hole so that the inlet pipe is in communication with the injection needle.

[0019] In one embodiment of this utility model, the pressure ring is provided with a first inclined surface, and the pipe joint is provided with a second inclined surface, the length of the second inclined surface being less than the length of the first inclined surface.

[0020] The beneficial effects of this utility model are:

[0021] The material delivery channel of this invention transports the sheath fluid flow to the flow chamber, while the injection needle simultaneously transports the sample flow to the flow chamber and mixes it with the sheath fluid flow to form a mixture. Through fluid dynamic focusing, the cells in the sample are encapsulated in the center of the sheath fluid. The combined sheath fluid flow and sample flow are then sequentially passed through the irradiation chamber and irradiated by a laser before being discharged through the discharge connector on the discharge pipe. The irradiation chamber and flow chamber are integrated into the flow cell component, eliminating the need for manual alignment during later assembly. This also effectively eliminates the problem of the irradiation chamber and flow chamber not being aligned or their relative positions shifting during use, which affects the fluid path performance. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of a flow cytometry device according to the present invention.

[0023] Figure 2 This is a cross-sectional view of the present invention.

[0024] The following are the labels in the diagram: 1. Base; 2. Flow cell component; 21. Column; 22. Shoulder; 23. Irradiation chamber; 24. Flow chamber; 25. Fixing block; 26. Conical hole; 27. Through hole; 3. Cover plate; 31. Positioning hole; 32. Limiting hole; 33. Fixing pin; 4. Discharge assembly; 41. Discharge rack; 42. Discharge connector; 43. Discharge pipe; 5. Feed connector; 51. Feed channel; 52. Sealing ring; 6. Mounting base; 61. Fixing nut; 62. Liquid inlet pipe; 63. Injection needle; 64. Fixing base; 65. Fixing hole; 66. First inclined surface; 67. Pipe connector; 68. Pressure ring; 69. Second inclined surface. Detailed Implementation

[0025] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, so that those skilled in the art can better understand and implement the present invention. However, the embodiments are not intended to limit the present invention.

[0026] Reference Figure 1-2 As shown, a flow cytometry device includes:

[0027] A base 1 is provided on which a flow cell component 2 is provided. A flow chamber 24 is provided at the lower part of the flow cell component 2, and an irradiation chamber 23 is provided at the top of the flow cell component 2. The flow chamber 24 is connected to the irradiation chamber 23.

[0028] The base 1 is provided with a material conveying channel 51, which is connected to the flow chamber 24. The material conveying channel 51 is used to convey the sheath fluid flow into the flow chamber 24.

[0029] The sample injection assembly is located below the base 1. The sample injection assembly includes a mounting base 6 and an injection needle 63. The injection needle 63 is connected to the base 1 through the mounting base 6. The discharge end of the injection needle 63 passes through the material conveying channel 51 and is located in the flow chamber 24. The injection needle 63 is used to transport the sample flow into the flow chamber 24 and mix it with the sheath fluid flow to form a mixture.

[0030] The discharge assembly 4 is connected to the flow cell component 2 and is connected to the irradiation chamber 23. The discharge assembly 4 is used to discharge the irradiated mixture in the irradiation chamber 23 as waste liquid.

[0031] The material delivery channel 51 of this invention delivers the sheath fluid flow to the flow chamber 24, while the injection needle 63 delivers the sample flow to the flow chamber 24 and mixes it with the sheath fluid flow to form a mixture. Through fluid dynamic focusing, the cells in the sample are encapsulated in the center of the sheath fluid. The combined sheath fluid flow and sample flow are then sequentially passed through the irradiation chamber 23 and irradiated by the laser before being discharged through the discharge connector 42 on the discharge pipe. The irradiation chamber 23 and the flow chamber 24 are integrated into the flow cell component 2, eliminating the need for manual alignment during later assembly. This also effectively eliminates the problem of the irradiation chamber 23 and the flow chamber 24 not being aligned or their relative positions shifting during use, which affects the fluid path effect.

[0032] In one embodiment of this utility model, the flow cell component 2 includes a column 21, a shoulder 22 is provided on the column 21, and a fixing block 25 is provided on the shoulder 22. The fixing block 25, the shoulder 22 and the column 21 are integrally formed. The irradiation chamber 23 is disposed in the fixing block 25. A cover plate 3 is provided on the base 1. The cover plate 3 is used to fix the column 21 to the base 1, and the shoulder 22 is engaged on the cover plate 3.

[0033] In one embodiment of this utility model, the shoulder platform 22 has a circular or polygonal cross-section, which can quickly position the flow cell component 2 and ensure the installation accuracy of the flow cell component 2 and the base 1. The cover plate 3 is provided with a positioning hole 31 that matches the shoulder platform 22. The shoulder platform 22 passes through the positioning hole 31. According to the optical path design requirements, the end face of the irradiation chamber 23 is usually glued with a lens. The laser is perpendicular to the end face of the irradiation chamber 23. Therefore, the flow cell should be fixed in the vertical direction (Z-axis rotation direction) relative to the optical base plate. When the flow cell component 2 is installed into the base 1, the shoulder platform 22 of the flow cell component 2 passes through the positioning hole 31 of the cover plate 3. Tighten the screws on the cover plate 3, and the flow cell component 2 is fixed between the cover plate 3 and the base 1. An O-ring is installed between the flow cell component 2 and the base 1 to prevent liquid leakage.

[0034] In one embodiment of this utility model, the cover plate 3 is provided with at least one limiting hole 32, and the bottom of the cover plate 3 is provided with a fixing pin 33. The fixing pin 33 is provided on both sides of the positioning hole 31. The cover plate 3 is equipped with fixing pin 33 so that it can be precisely positioned and connected with the optical base plate. The fixing parts such as bolts pass through the limiting hole 32 to quickly connect with the optical base plate, thereby improving the assembly accuracy and ensuring the assembly efficiency.

[0035] In one embodiment of this utility model, the column 21 has a conical cylindrical structure, and the base 1 is provided with a conical hole 26 that matches the column 21. The conical hole 26 is provided with a through hole 27 that communicates with the material conveying channel 51, and the column 21 is sealed to the through hole 27.

[0036] Specifically, the column 21 has a conical cylindrical structure. The column 21 cooperates with the conical hole 26 of the base 1, so that the flow cell and the base 1 can better self-align during assembly, effectively eliminating the machining accuracy problems caused by direct machining of the cylindrical 21, ensuring the assembly accuracy of the flow cell component 2 and the base 1, and effectively avoiding problems that affect the liquid circuit effect.

[0037] In one embodiment of this utility model, the discharge assembly 4 includes a discharge rack 41, which is disposed on the cover plate 3. A pressure block is disposed on the discharge rack 41, which is connected to the flow cell component 2. A discharge pipe is disposed on the pressure block, which is connected to the irradiation chamber 23. A discharge connector 42 is disposed on the discharge pipe 43. The converging sheath fluid flow and sample flow are sequentially irradiated by the laser in the irradiation chamber 23 and then discharged through the discharge connector 42 on the discharge pipe. This allows for easy assembly of accessories such as conduits and is convenient to use.

[0038] In one embodiment of this utility model, the feed end of the conveying channel 51 is provided with an internal threaded hole, which is connected to the conveying connector 5. A sealing ring 52 is provided between the conveying connector 5 and the base 1. The conveying connector 5 guides the sheath fluid flow through the conveying channel 51 and the through hole 27 into the flow chamber 24 for confluence, which can facilitate the flow of sheath fluid into the flow chamber 24 and improve the confluence efficiency.

[0039] In one embodiment of the present invention, the injection needle 63 is disposed on the fixed seat 64, the fixed seat 64 has a conical columnar structure, the lower part of the base 1 is provided with a fixing hole 65 that matches the fixed seat 64, the fixed seat 64 is sealed to the fixing hole 65, and the fixed seat 64 is connected to the base 1 by a fixing nut 61.

[0040] Specifically, after the injection needle 63 is placed in position on the fixing base 64, it is bonded together. Since the fixing base 64 has a conical columnar structure, it connects to the fixing hole 65 at the bottom of the base 1, allowing the two to self-align during assembly. This ensures that the injection needle 63 and the center of the flow cell are aligned, reducing assembly difficulty while guaranteeing a sealing effect.

[0041] In one embodiment of the present invention, the mounting base 6 includes a pipe connector 67, a pressure ring 68 is provided on the pipe connector 67, the pressure ring 68 is made of rubber material, an inlet pipe 62 is provided on the pressure ring 68, the fixing base 64 is provided with an inlet hole, and the pipe connector 67 is inserted into the inlet hole so that the inlet pipe 62 is in communication with the injection needle 63.

[0042] Specifically, the pipe connector 67 is inserted into the liquid inlet hole so that the liquid inlet tube 62 is connected to the injection needle 63. The pressure ring 68 is made of rubber material, which can seal the connection between the fixing seat 64 and the pipe connector 67, ensuring quick assembly and disassembly of the two, and is practical.

[0043] In one embodiment of the present invention, the pressure ring 68 is provided with a first inclined surface 66, and the pipe joint 67 is provided with a second inclined surface 69, wherein the length of the second inclined surface 69 is less than the length of the first inclined surface 66.

[0044] Specifically, the pipe connector 67 is inserted into the liquid inlet hole. Since the length of the second inclined surface 69 is less than the length of the first inclined surface 66, the second inclined surface 69 on the pipe connector 67 can squeeze the first inclined surface 66 of the pressure ring 68, causing the pressure ring 68 to deform and press the liquid inlet pipe 62 onto the pressure ring 68, thereby quickly fixing the liquid inlet pipe 62. This makes it convenient and quick to use.

[0045] Usage process

[0046] Insert the pipe connector 67 into the liquid inlet. Since the length of the second inclined surface 69 is less than the length of the first inclined surface 66, the second inclined surface 69 on the pipe connector 67 can squeeze the first inclined surface 66 of the pressure ring 68, so that the liquid inlet pipe 62 is pressed on the pressure ring 68, making the liquid inlet pipe 62 connected to the injection needle 63. Tighten the fixing nut 61 to fix the fixing seat 64 of the injection needle 63 to the fixing of the base 1. The sample flows into the flow chamber 24 through the liquid inlet pipe 62. At the same time, the feed connector 5 draws the sheath fluid flow into the flow chamber 24 through the feed channel 51 and the through hole 27 for convergence. After fluid dynamic focusing, the cells in the sample are encapsulated in the center of the sheath fluid. Then, the converged sheath fluid flow and sample flow are sequentially irradiated by the laser through the irradiation chamber 23 and discharged through the discharge connector 42 on the discharge pipe.

[0047] The above-described embodiments are merely preferred embodiments provided to fully illustrate the present invention, and the scope of protection of the present invention is not limited thereto. Equivalent substitutions or modifications made by those skilled in the art based on the present invention are all within the scope of protection of the present invention. The scope of protection of the present invention is defined by the claims.

Claims

1. A flow cytometry apparatus, characterized by, include: A base on which a flow cell component is provided, a flow chamber is provided at the lower part of the flow cell component, and an irradiation chamber is provided at the top of the flow cell component, the flow chamber being connected to the irradiation chamber; The base is provided with a material conveying channel, which is connected to the flow chamber and is used to convey the sheath fluid flow into the flow chamber; A sample injection assembly is disposed below the base. The sample injection assembly includes a mounting base and a sample injection needle. The sample injection needle is connected to the base through the mounting base. The discharge end of the sample injection needle passes through the material delivery channel and is located in the flow chamber. The sample injection needle is used to deliver the sample flow into the flow chamber and mix it with the sheath fluid flow to form a mixture. The discharge assembly is connected to the flow cell component and is connected to the irradiation chamber. The discharge assembly is used to discharge the irradiated mixture from the irradiation chamber as waste liquid.

2. The flow cytometry apparatus of claim 1, wherein, The flow cell component includes a column, a shoulder platform on the column, a fixing block on the shoulder platform, the fixing block, the shoulder platform, and the column are integrally formed, the irradiation chamber is disposed inside the fixing block, and a cover plate is disposed on the base. The cover plate is used to fix the column to the base, and the shoulder platform is engaged with the cover plate.

3. The flow cytometry apparatus of claim 2, wherein, The shoulder has a circular or polygonal cross-section, and the cover plate is provided with positioning holes that match the shoulder, with the shoulder passing through the positioning holes.

4. The flow cytometry apparatus of claim 3, wherein, The cover plate is provided with at least one limiting hole, and a fixing pin is provided on the bottom of the cover plate, with the fixing pin located on both sides of the positioning hole.

5. The flow cytometry apparatus of claim 2, wherein, The column has a conical cylindrical structure, and the base is provided with a conical hole that matches the column. The conical hole is provided with a through hole that communicates with the material conveying channel, and the column is sealed to the through hole.

6. The flow cytometry apparatus of claim 2, wherein, The discharge assembly includes a discharge rack, which is disposed on the cover plate. A pressure block is disposed on the discharge rack and connected to the flow cell component. A discharge pipe is disposed on the pressure block and connected to the irradiation chamber. A discharge connector is disposed on the discharge pipe.

7. The flow cytometry apparatus of claim 1, wherein, The feed end of the material conveying channel is provided with an internal threaded hole, which is connected to the material conveying connector. A sealing ring is provided between the material conveying connector and the base.

8. The flow cytometry apparatus of claim 1, wherein, The injection needle is mounted on a fixed base, which has a conical columnar structure. The lower part of the base has a fixing hole that matches the fixed base. The fixed base is sealed to the fixing hole, and the fixed base is connected to the base by a fixing nut.

9. The flow cytometry apparatus of claim 8, wherein, The mounting base includes a pipe connector with a pressure ring made of rubber. An inlet pipe passes through the pressure ring. The mounting base has an inlet hole. The pipe connector is inserted into the inlet hole so that the inlet pipe communicates with the injection needle.

10. The flow cytometry apparatus of claim 9, wherein, The pressure ring is provided with a first inclined surface, and the pipe joint is provided with a second inclined surface, the length of the second inclined surface being less than the length of the first inclined surface.

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