Circulating tumor cell screening and separating device based on SERS (Surface Enhanced Raman Scattering) technology

Abstract

The utility model provides a circulating tumor cell screening and separating device based on an SERS (Surface Enhanced Raman Scattering) technology, which belongs to the technical field of medical detection and comprises a bracket, a circulating tumor cell screening and separating device and a circulating tumor cell screening and separating device, the linear driving element is fixedly connected with the bracket; the reaction chamber is mounted on the sliding seat; the pipetting mechanism is mounted in the reaction chamber; when the reaction chamber is located at the working position, the linear driving element is detachably connected with the pipetting mechanism; when the linear driving element is separated from the pipetting mechanism, the reaction chamber is allowed to move along with the sliding seat; and the filtering collector is detachably connected with the bracket through a buckle. The screening and separating device has the beneficial effects that the screening and separating device is high in integration level and compact in structure, the reaction chamber can move to an operation position through the sliding seat to add a sample and a nano magnetic probe, and then moves to a working position to be connected with the linear driving element, so that the pipetting and cleaning processes are automatically controlled; and the filter collector can be taken out from the buckle to facilitate subsequent detection.

Description

Technical Field

[0001] This utility model belongs to the field of medical testing technology and relates to a circulating tumor cell screening and separation device based on SERS technology. Background Technology

[0002] Circulating tumor cells (CTCs) are tumor cells that detach from primary or metastatic tumors and enter the bloodstream. CTC detection is crucial for early cancer diagnosis, disease monitoring, treatment evaluation, and prognosis. CTCs are extremely rare in blood (only 0-100 CTCs per mL), making the enrichment and detection of CTCs from the blood a key challenge. Among various enrichment methods, immunomagnetic bead assays are widely used due to their rapid and non-destructive separation. This method relies on the binding of CTC surface-specific antigens to antibodies on magnetic beads, achieving enrichment through an external magnetic field, followed by detection using surface-enhanced Raman scattering (SERS) technology.

[0003] Previously, the screening and separation of CTCs in blood usually relied on manual operation, which was cumbersome and inefficient. Although some devices based on immunomagnetic bead separation technology for screening and separating CTCs exist, these devices have low functional integration, the various components are not integrated together, the overall structure is not compact, and the operation is also relatively cumbersome, leaving room for improvement. Utility Model Content

[0004] The purpose of this invention is to address the aforementioned problems in the existing technology by proposing a circulating tumor cell screening and separation device based on SERS technology.

[0005] The objective of this utility model can be achieved through the following technical solution: a circulating tumor cell screening and separation device based on SERS technology, comprising:

[0006] The bracket is equipped with a buckle and a movable sliding seat;

[0007] A linear drive element, wherein the linear drive element is fixedly connected to the bracket;

[0008] A reaction chamber is mounted on the sliding seat, and the reaction chamber can be moved to a working position or an operating position via the sliding seat;

[0009] A pipetting mechanism is installed in the reaction chamber and can control the liquid transfer in the reaction chamber through its own movement. When the reaction chamber is in the working position, the linear drive element is detachably connected to the pipetting mechanism. When the linear drive element is separated from the pipetting mechanism, the reaction chamber is allowed to move with the sliding seat.

[0010] A filter collector is detachably connected to the bracket via a snap fastener, and the outlet of the reaction chamber and the inlet of the filter collector are connected via a pipeline.

[0011] Preferably, the device also includes a housing and a cover, with the bracket fixedly installed inside the housing. The linear drive element, the reaction chamber, and the filter collector are all located inside the housing. The housing has an opening corresponding to the area where the reaction chamber and the filter collector are located. The cover is hinged to the housing and can cover the opening.

[0012] Preferably, the outer wall of the reaction chamber is provided with a heating jacket.

[0013] Preferably, the buckle includes two elastic clamping arms, which hold the filter collector in place when the buckle is detachably connected to the filter collector.

[0014] Preferably, the shape of the elastic clamping arm is complementary to the side profile of the filter collector.

[0015] Preferably, the pipetting mechanism includes a piston rod and a piston block. The piston rod passes through the top of the reaction chamber, and the piston block is movably disposed within the reaction chamber. The piston block is sealed against the cavity wall of the reaction chamber and divides the interior of the reaction chamber into an upper cavity and a lower cavity. One end of the piston rod is connected to the piston block, and the other end of the piston rod is detachably connected to the linear drive element.

[0016] Preferably, when the reaction chamber is in the working position, the linear drive element and the piston rod are arranged vertically correspondingly, and the linear drive element and the pipetting mechanism are axially locked by a pin or screw.

[0017] Preferably, the outer wall of the reaction chamber is provided with a first connector and a second connector, the first connector and the second connector being connected to the upper cavity and the lower cavity respectively, and the first connector and the second connector being connected through a liquid guide tube.

[0018] Preferably, the filter collector includes a base, a top cover, a filter membrane, and a porous support plate. The porous support plate is disposed on the base, the filter membrane is disposed on the porous support plate, the top cover is detachably installed on the base and presses down on the filter membrane, and the top cover and the base are respectively connected to an inlet connector and an outlet connector.

[0019] Preferably, it also includes a glass tube, wherein a magnetic element is disposed inside the glass tube, and the glass tube is detachably connected to the top of the reaction chamber.

[0020] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0021] 1. A highly integrated and compact screening and separation device is provided. The reaction chamber can be moved to the operation position by a sliding seat to add samples and nanomagnetic probes, and then moved to the working position to connect with the linear drive element to automatically control the pipetting and washing process. The filter collector can be removed from the snap-fit ​​for subsequent detection.

[0022] 2. The heating jacket can heat the inside of the reaction chamber and maintain the temperature inside the reaction chamber at a set value (generally around 37°C), thereby simulating the constant temperature environment of the human body and ensuring that the cells react under the most suitable conditions.

[0023] 3. The drainage function is achieved by controlling the movement of the piston block through a linear drive element, which greatly improves the automation and convenience of the device. This design can automatically control drainage after the reaction and culture, and automatically perform cleaning during the cleaning stage. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the screening and separation device of this utility model.

[0025] Figure 2 This is a schematic diagram of the outer shell and cover of this utility model.

[0026] Figure 3 This is a schematic diagram showing the reaction chamber, linear drive element, and filter collector of this utility model mounted on a support.

[0027] Figure 4 This is an exploded view of the structure of the reaction chamber, linear drive element, and filter collector of this utility model mounted on the support.

[0028] Figure 5 This is a schematic diagram of the internal structure of the reaction chamber and filter collector of this utility model.

[0029] Figure 6 This is a half-sectional schematic diagram of the screening and separation device of this utility model.

[0030] In the diagram, 100 is the support; 110 is the buckle; 111 is the elastic clamping arm; 120 is the sliding seat; 200 is the linear drive element; 300 is the reaction chamber; 310 is the heating jacket; 320 is the upper chamber; 330 is the lower chamber; 340 is the first connector; 350 is the second connector; 400 is the pipetting mechanism; 410 is the piston rod; 420 is the piston block; 500 is the filter collector; 510 is the base; 511 is the outlet connector; 520 is the top cover; 521 is the inlet connector; 530 is the filter membrane; 540 is the porous support plate; 600 is the outer shell; 610 is the cap; and 700 is the glass tube. Detailed Implementation

[0031] The following are specific embodiments of the present invention, which are described in conjunction with the accompanying drawings. However, the present invention is not limited to these embodiments.

[0032] like Figures 1 to 6 As shown, a circulating tumor cell screening and separation device based on SERS technology includes: a support 100, which is provided with a buckle 110 and a movable sliding seat 120; a linear drive element 200, which is fixedly connected to the support 100; a reaction chamber 300, which is mounted on the sliding seat 120 and can be moved to a working position or an operating position via the sliding seat 120; and a pipetting mechanism 400, which is mounted on the reaction chamber 300 and can transfer liquids... The liquid transfer mechanism 400 can control the liquid transfer in the reaction chamber 300 through its own motion state; when the reaction chamber 300 is in the working position, the linear drive element 200 is detachably connected to the liquid transfer mechanism 400; when the linear drive element 200 is separated from the liquid transfer mechanism 400, the reaction chamber 300 is allowed to move with the sliding seat 120; the filter collector 500 is detachably connected to the bracket 100 through the snap 110, and the outlet of the reaction chamber 300 and the inlet of the filter collector 500 are connected by a pipeline.

[0033] This screening and separation device is based on immunomagnetic bead separation (IMS) technology for screening and separating circulating tumor cells (CTCs). The target cells (circulating tumor cells) separated and screened using this device can be detected by surface-enhanced Raman scattering (SERS) technology. This device provides a reaction culture space for the binding of nanomagnetic probes (magnetic SERS probes) with circulating tumor cells. An external magnetic field attracts and immobilizes these nanomagnetic probes bound to the target cells, while unbound components are eluted and removed by solution, thus achieving effective separation of target cells from other components.

[0034] The support 100 provides mounting positions for other components, allowing the linear drive element 200, reaction chamber 300, and filter collector 500 to be integrated. A snap-fit ​​110 secures the filter collector 500, while a sliding seat 120 allows for positional adjustment of the reaction chamber 300. The linear drive element 200 is preferably an electric actuator, providing power to the pipetting mechanism 400. The reaction chamber 300 performs multiple functions in different operational stages. During the reaction incubation stage, it provides a reaction environment for the sample and the nanomagnetic probe. Subsequent enrichment and washing stages also take place within the reaction chamber 300. The top of the reaction chamber 300 has an inlet port that communicates with the chamber, allowing blood samples, nanomagnetic probes, and washing solution (PBS buffer) to enter the chamber. The bottom of the reaction chamber 300 has a drain port that connects to the inlet port of the filter collector 500 via tubing.

[0035] The main function of the pipetting mechanism 400 is to control the liquid flow within the reaction chamber 300 and the connection between the reaction chamber 300 and the filter collector 500. During the reaction incubation stage, the pipetting mechanism 400 isolates the flow path between the reaction chamber 300 and the filter collector 500, allowing the sample and the nanomagnetic probe to react and incubate within the reaction chamber 300. During the enrichment stage, it discharges waste liquid to the filter collector 500; during the washing stage, it discharges washing liquid to the filter collector 500. The filter collector 500 is detachably connected to the support 100 via a snap-fit ​​110. During the washing stage, the enriched target cells flow into the filter collector 500 through the tubing under the flushing effect of the washing liquid. The filter collector 500 can intercept target cells (CTCs) carrying nanomagnetic probes.

[0036] The reaction chamber 300 in this device is mounted on a movable sliding seat 120, a design that allows the operator to push the reaction chamber 300 to either the operating or working position. In the operating position, the operator can directly add blood samples and nanomagnetic probes to the reaction chamber 300. These probes are used to specifically bind to target cells (e.g., CTCs) for subsequent screening and separation processes. When the reaction chamber 300 is moved to the working position, the pipetting mechanism 400 can be locked together with the linear drive element 200. The linear drive element 200 then powers the pipetting mechanism 400, enabling it to automatically perform liquid transfer and washing operations. The filter collector 500 can be easily connected to or detached from the support 100 via a snap-fit ​​110. After the washing step, the target cells are retained in the filter collector 500 through the filter membrane 530. Since the filter collector 500 can be removed from the support 100, this greatly facilitates subsequent surface-enhanced Raman scattering (SERS) detection.

[0037] Therefore, this design has a high degree of integration and the overall equipment is more compact. Key components (such as reaction chamber 300 and filter collector 500) can be moved or disassembled according to the requirements of the test steps, which is very convenient.

[0038] like Figure 1 , Figure 2 , Figure 5 As shown, based on the above embodiment, it also includes a housing 600 and a cover 610. The bracket 100 is fixedly installed inside the housing 600. The linear drive element 200, the reaction chamber 300 and the filter collector 500 are all located inside the housing 600. The housing 600 is provided with an opening, which corresponds to the area where the reaction chamber 300 and the filter collector 500 are located. The cover 610 is hinged to the housing 600 and can cover the opening.

[0039] The housing 600 provides physical protection for the internal components, preventing dust, moisture, and other contaminants from entering the device, which is crucial for maintaining long-term stable operation. The opening of the housing 600 is located on the front side, a design that facilitates the removal of the reaction chamber 300 and the filter collector 500; during operation, the opening is covered by the cap 610 (i.e., the cap 610 is in the closed state), thereby allowing the reaction chamber 300, the pipetting mechanism 400, the linear drive element 200, and the filter collector 500 to operate automatically within a relatively enclosed space.

[0040] like Figure 1 , Figure 5 , Figure 6 As shown, based on the above embodiment, a heating jacket 310 is provided on the outer wall of the reaction chamber 300. The heating jacket 310 can heat the interior of the reaction chamber 300 and maintain the temperature inside the reaction chamber 300 at a set value (generally maintained at around 37°C), thereby simulating the constant temperature environment of the human body and ensuring that the cells react under the most suitable conditions. Without the heating jacket 310, a suitable reaction environment cannot be provided inside the reaction chamber 300, which may lead to unsatisfactory reaction culture results; in addition, the lack of the heating jacket 310 means that the temperature inside the reaction chamber 300 may fluctuate with changes in the external environment, resulting in inconsistent reaction culture conditions and thus affecting the reliability of the detection results.

[0041] like Figure 1 , Figure 3 , Figure 4 As shown, based on the above embodiment, the buckle 110 includes two elastic clamping arms 111. When the buckle 110 is detachably connected to the filter collector 500, the two elastic clamping arms 111 hold the filter collector 500.

[0042] Two elastic clamping arms 111 clamp and fix the filter collector 500 from both sides, forming a symmetrical force, which can provide a uniform and stable clamping force. The elastic clamping arms 111 have a certain deformation capability. The operator only needs to insert the filter collector 500 into the buckle 110 area, and the elastic arms will automatically open and spring back to clamp. When removing, only a little external force is needed to detach it.

[0043] Based on the above embodiment, the shape of the elastic clamping arm 111 is complementary to the side profile of the filter collector 500. This complementary shape means that the elastic clamping arm 111 can fit more closely to the side profile of the filter collector 500, thus providing a better clamping effect.

[0044] like Figure 1 , Figure 3 , Figure 4 , Figure 5 , Figure 6 As shown, based on the above embodiment, the pipetting mechanism 400 includes a piston rod 410 and a piston block 420. The piston rod 410 passes through the top of the reaction chamber 300, and the piston block 420 is movably disposed inside the reaction chamber 300. The piston block 420 is sealed to the cavity wall of the reaction chamber 300 and divides the interior of the reaction chamber 300 into an upper cavity 320 and a lower cavity 330. One end of the piston rod 410 is connected to the piston block 420, and the other end of the piston rod 410 is detachably connected to the linear drive element 200.

[0045] The upper chamber 320 provides the reaction incubation environment. Blood samples and nanomagnetic probes are placed into the upper chamber 320. The lower chamber 330 is connected to the filter collector 500. Liquid in the upper chamber 320 flows into the filter collector 500 through the lower chamber 330. The linear drive element 200 can drive the piston block 420 to move up and down via the piston rod 410. When the piston block 420 moves upward, the volume in the upper chamber 320 decreases, and the liquid in the upper chamber 320 flows into the lower chamber 330, and then into the filter collector 500. When the piston block 420 moves downward, the volume of the upper chamber 320 increases, preparing for the next pipetting.

[0046] Specifically, the main function of piston block 420 is to control the flow path between upper chamber 320 and lower chamber 330 and to force liquid transfer through its own movement. When piston block 420 is stationary, the flow path between upper chamber 320 and lower chamber 330 is closed. At this time, liquid can remain in upper chamber 320, but the liquid cannot flow into lower chamber 330. Therefore, during the reaction incubation stage, piston block 420 remains stationary, and the nanomagnetic probe and blood sample can undergo reaction incubation in upper chamber 320. During the enrichment and washing stages, piston block 420 moves upward, compressing the liquid in upper chamber 320 and transferring it into lower chamber 330; then piston block 420 moves downward, thereby draining the liquid in lower chamber 330 through the pipeline to filter collector 500.

[0047] The liquid drainage function is achieved by controlling the movement of the piston block 420 through the linear drive element 200, which greatly improves the automation level and convenience of the device. This design can automatically control the liquid drainage after the reaction and culture, and can automatically perform cleaning during the cleaning stage.

[0048] Based on the above embodiments, when the reaction chamber 300 is in the working position, the linear drive element 200 and the piston rod 410 are arranged vertically and vertically respectively, and the linear drive element 200 and the pipetting mechanism 400 are axially locked by pins or screws.

[0049] The linear drive element 200 and the pipetting mechanism 400 can be axially locked together by pins or screws, so that the linear drive element 200 can drive the piston block 420 to move through the piston rod 410. The connection between the linear drive element 200 and the pipetting mechanism 400 is both firm and easy to disassemble and assemble.

[0050] Based on the above embodiments, the outer wall of the reaction chamber 300 is provided with a first connector 340 and a second connector 350. The first connector 340 and the second connector 350 are respectively connected to the upper cavity 320 and the lower cavity 330, and the first connector 340 and the second connector 350 are connected through a liquid guide tube.

[0051] The liquid guide tube serves as the flow path between the upper chamber 320 and the lower chamber 330. When the piston block 420 moves upward, the pressure in the upper chamber 320 increases, so the liquid in the upper chamber 320 enters the lower chamber 330 through the liquid guide tube; when the piston block 420 moves downward, the pressure in the lower chamber 330 increases, so the liquid in the lower chamber 330 is discharged towards the filter collector 500.

[0052] In the initial state, the piston block 420 is at the bottom of the reaction chamber 300, maximizing the volume of the upper chamber 320. At this point, blood samples and nano-magnetic probes can be placed into the upper chamber 320 for reaction and incubation. When drainage is required, the piston block 420 is pulled upwards, allowing the liquid in the upper chamber 320 to flow into the lower chamber 330 through the guide tube. Once the piston block 420 reaches a predetermined position, the linear drive element 200 moves it downwards, causing the liquid in the lower chamber 330 to drain towards the filter collector 500. The piston block 420 can be repeatedly pulled to achieve drainage or cleaning.

[0053] like Figure 1 , Figure 3 , Figure 4 , Figure 5 , Figure 6 As shown, based on the above embodiment, the filter collector 500 includes a base 510, a top cover 520, a filter membrane 530, and a porous support plate 540. The porous support plate 540 is disposed on the base 510, the filter membrane 530 is disposed on the porous support plate 540, the top cover 520 is detachably installed on the base 510 and presses down the filter membrane 530, and the top cover 520 and the base 510 are respectively connected to an inlet connector 521 and an outlet connector 511.

[0054] A sealed cavity is formed between the top cover 520 and the base 510. The porous support plate 540 and the filter membrane 530 are located in the area between the inlet connector 521 and the outlet connector 511. Liquid can flow into the filter collector 500 from the inlet connector 521. During the cleaning process, the CTCs-magnetic bead complex in the liquid cannot pass through the filter membrane 530 and is thus intercepted on the surface of the filter membrane 530.

[0055] After cleaning, remove the filter collector 500 from the clip 110, then remove the top cover 520 from the base 510, replace the glass window, and then take it to the Raman spectrometer for testing.

[0056] This detachable design enables in-situ Raman detection. After the cleaning stage, there is no need to remove the filter membrane 530 or CTCs-magnetic bead composite from the base 510. Instead, the filter collector 500 can be cleverly removed from the device separately. After installing the glass window, the filter collector 500 can be placed directly on the detection platform of the Raman spectrometer, avoiding contamination that may be introduced during the transfer process and improving the accuracy and reliability of the detection results.

[0057] like Figure 5 As shown, based on the above embodiment, it also includes a glass tube 700, which is provided with a magnetic component and is detachably connected to the top of the reaction chamber 300.

[0058] The glass tube 700 and the magnetic component are used to generate a magnetic field within the reaction chamber 300 to adsorb target cells (CTCs) carrying nanomagnetic probes, thereby achieving the enrichment of CTCs-magnetic bead complexes and their separation from other cells.

[0059] like Figures 1 to 6 As shown, the actual working steps of this device are as follows:

[0060] 1. Open the cover 610 of the outer shell 600, then pull the reaction chamber 300 from the opening to the operating position, and put the nanomagnetic probe and blood sample into the upper cavity 320 of the reaction chamber 300. Then push the reaction chamber 300 back to the working position and lock the piston rod 410 and the linear drive element 200 together with screws or pins.

[0061] 2. Press the start button to heat the liquid in the reaction chamber 300 under the heating jacket 310. The reaction chamber 300 simulates the constant temperature environment of the human body. During the reaction culture process, the CTCs in the blood sample and the nanomagnetic probe combine to form CTCs-magnetic bead complex.

[0062] 3. After the reaction incubation process reaches the set time, the glass tube 700 pre-loaded with magnetic material is inserted into the top interface of the reaction chamber 300. The magnetic field generated by the magnetic material adsorbs the CTCs-magnetic bead composite onto the outer wall of the glass tube 700. This process is the enrichment process.

[0063] 4. The linear drive element 200 drives the piston block 420 to move up and down repeatedly, thereby discharging the liquid in the reaction chamber 300;

[0064] 5. Remove the magnetic component from the glass tube 700, and then inject cleaning fluid into the upper cavity 320 to rinse off the CTCs-magnetic bead composite on the outer wall of the glass tube 700. During this process, an ultrasonic element can be inserted to clean the upper cavity 320. This process is called the cleaning process.

[0065] 6. The linear drive element 200 drives the piston block 420 to move up and down repeatedly, discharging the washing liquid in the reaction chamber 300 to the filter collector 500. The CTCs-magnetic bead complex in the washing liquid is blocked by the filter membrane 530. This process is a screening and separation process.

[0066] 7. Remove the filter collector 500 from the clip 110, replace the top cover 520 with a glass window, and then place it under a Raman spectrometer for detection.

[0067] This device is highly automated, and the entire operation can be started with a single button, reducing manual intervention and improving operational efficiency. Furthermore, its compact structure integrates multiple functions such as reaction cultivation, enrichment, and cleaning, ensuring all operations are completed in a closed environment. The reaction chamber 300 can also be moved or the filter collector 500 can be disassembled as needed to facilitate manual operation of specific steps.

[0068] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in this utility model embodiment are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0069] Furthermore, in this utility model, the use of terms such as "first," "second," and "a" is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0070] In this utility model, unless otherwise explicitly specified and limited, the terms "connection," "fixing," etc., should be interpreted broadly. For example, "fixing" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0071] Furthermore, the technical solutions of the various embodiments of this utility model can be combined with each other, but only if they are based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

Claims

1. A circulating tumor cell screening and separation device based on SERS technology, characterized in that, include: A bracket (100) is provided with a buckle (110) and a movable sliding seat (120); A linear drive element (200) is fixedly connected to the bracket (100); A reaction chamber (300) is mounted on the sliding seat (120), and the reaction chamber (300) can be moved to a working position or an operating position via the sliding seat (120); A pipetting mechanism (400) is installed in the reaction chamber (300). The pipetting mechanism (400) can control the liquid transfer in the reaction chamber (300) through its own movement. When the reaction chamber (300) is in the working position, the linear drive element (200) is detachably connected to the pipetting mechanism (400). When the linear drive element (200) is separated from the pipetting mechanism (400), the reaction chamber (300) is allowed to move with the sliding seat (120). A filter collector (500) is detachably connected to the bracket (100) via the buckle (110), and the outlet of the reaction chamber (300) and the inlet of the filter collector (500) are connected by a pipeline.

2. The circulating tumor cell screening and separation device based on SERS technology as described in claim 1, characterized in that: It also includes a housing (600) and a cover (610). The bracket (100) is fixedly installed inside the housing (600). The linear drive element (200), the reaction chamber (300), and the filter collector (500) are all located inside the housing (600). The housing (600) is provided with an opening corresponding to the area where the reaction chamber (300) and the filter collector (500) are located. The cover (610) is hinged to the housing (600) and can cover the opening.

3. The circulating tumor cell screening and separation device based on SERS technology as described in claim 1, characterized in that: The outer wall of the reaction chamber (300) is provided with a heating jacket (310).

4. The circulating tumor cell screening and separation device based on SERS technology as described in claim 1, characterized in that: The buckle (110) includes two elastic clamping arms (111). When the buckle (110) is detachably connected to the filter collector (500), the two elastic clamping arms (111) clamp the filter collector (500).

5. The circulating tumor cell screening and separation device based on SERS technology as described in claim 4, characterized in that: The shape of the elastic clamping arm (111) is complementary to the side profile of the filter collector (500).

6. The circulating tumor cell screening and separation device based on SERS technology as described in claim 1, characterized in that: The pipetting mechanism (400) includes a piston rod (410) and a piston block (420). The piston rod (410) passes through the top of the reaction chamber (300), and the piston block (420) is movably disposed in the reaction chamber (300). The piston block (420) is sealed to the cavity wall of the reaction chamber (300) and divides the interior of the reaction chamber (300) into an upper cavity (320) and a lower cavity (330). One end of the piston rod (410) is connected to the piston block (420), and the other end of the piston rod (410) is detachably connected to the linear drive element (200).

7. The circulating tumor cell screening and separation device based on SERS technology as described in claim 6, characterized in that: When the reaction chamber (300) is in the working position, the linear drive element (200) and the piston rod (410) are arranged vertically in correspondence, and the linear drive element (200) and the pipetting mechanism (400) are axially locked by pins or screws.

8. The circulating tumor cell screening and separation device based on SERS technology as described in claim 6, characterized in that: The outer wall of the reaction chamber (300) is provided with a first connector (340) and a second connector (350). The first connector (340) and the second connector (350) are respectively connected to the upper cavity (320) and the lower cavity (330). The first connector (340) and the second connector (350) are connected through a liquid guide tube.

9. A circulating tumor cell screening and separation device based on SERS technology as described in claim 1 or 4, characterized in that: The filter collector (500) includes a base (510), a top cover (520), a filter membrane (530), and a porous support plate (540). The porous support plate (540) is disposed on the base (510), the filter membrane (530) is disposed on the porous support plate (540), and the top cover (520) is detachably installed on the base (510) and presses down the filter membrane (530). The top cover (520) and the base (510) are respectively connected to an inlet connector (521) and an outlet connector (511).

10. The circulating tumor cell screening and separation device based on SERS technology as described in claim 1, characterized in that: It also includes a glass tube (700) with a magnetic component inside, and the glass tube (700) is detachably connected to the top of the reaction chamber (300).

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