Cell reaction liquid sampling equipment

Abstract

The utility model relates to the technical field of reaction liquid sampling, and discloses cell reaction liquid sampling equipment which comprises a top plate and a measuring cylinder, a stirring assembly is arranged at the bottom of the top plate, and a quantifying assembly is arranged in the measuring cylinder; the stirring assembly comprises a fixing column, the fixing column is sleeved with a first rotating ring, a second rotating ring and a rotating plate, a gear plate is slidably embedded in the rotating plate, a power rod is rotatably embedded in the top plate, a fixing rod is rotatably embedded in the power rod, and the fixing rod is rotatably embedded in the gear plate; the quantifying assembly comprises a measuring cylinder, a large piston is slidably embedded in the measuring cylinder, a sliding rod is slidably embedded in the measuring cylinder, and an air cylinder is arranged on the other side of the sliding rod, so that the problems that stirring is not carried out in the using process, and quantitative extraction cannot be carried out are solved.

Description

Technical Field

[0001] This utility model relates to the field of reaction solution sampling technology, specifically a cell reaction solution sampling device. Background Technology

[0002] Cell reaction solution is a liquid culture medium used to support cell growth, metabolism, and function. It typically contains nutrients, growth factors, hormones, and possible metabolites required for cell growth, providing a suitable growth environment for cells.

[0003] Chinese Patent Publication No. CN208488283U discloses a "Reaction Liquid Sampling Device," comprising a main body, an inlet located at the top of the main body, a hatch located within the main body, and a sampling bottle disposed within the main body. The hatch is hinged to the main body. An inlet pipe is provided in the inlet, and a sampling bottle fixing device is fixedly connected to the end of the inlet pipe inserted into the main body. The sampling bottle is disposed within the sampling bottle fixing device. The hatch is equipped with a viewing window and a vent valve. This invention provides a reaction liquid sampling device with a simple structure and convenient sampling. The tempered glass viewing window on the hatch facilitates observation of the liquid's inflow. Sampling can be performed without opening the cap or depressurizing; the liquid can be pumped out using the internal pressure of the reactor, making it more environmentally friendly.

[0004] While existing technologies can collect liquid samples, cells may settle to the bottom of the reactor or clump together during use, resulting in uneven cell distribution and inaccurate sampling results. This can mislead subsequent analysis and testing, affecting experiments or production. Furthermore, the inability to quantitatively control the sampling volume during use leads to inaccurate sampling amounts, which in turn affects the repeatability and reliability of the experimental or production process. Summary of the Invention

[0005] The purpose of this invention is to provide a cell reaction solution sampling device to solve the problems in the background art where, during use, cells may settle to the bottom of the reactor or aggregate into clumps, resulting in uneven cell distribution, inaccurate sampling results, misleading subsequent analysis and detection, and affecting experiments or production. At the same time, the inability to quantitatively control the sampling during use leads to inaccurate sampling volume, which in turn affects the repeatability and reliability of the experimental or production process.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a cell reaction solution sampling device, including a top plate and a graduated cylinder, wherein a stirring assembly is provided at the bottom of the top plate and a quantitative assembly is provided inside the graduated cylinder;

[0007] The stirring assembly includes a fixed column, which is fixedly connected to the bottom of the outer surface of the top plate. A first rotating ring is rotatably sleeved on the outer surface of the fixed column, a second rotating ring is rotatably sleeved on the outer surface of the fixed column, a rotating plate is rotatably sleeved on the outer surface of the fixed column, and a gear plate is slidably embedded inside the rotating plate. A power rod is rotatably embedded inside the top plate, and a fixed rod is rotatably embedded inside the power rod. The fixed rod is rotatably embedded inside the gear plate.

[0008] The quantitative component includes a measuring cylinder, and a large piston is slidably embedded inside the measuring cylinder. A slide rod is slidably embedded inside the measuring cylinder, and the slide rod is fixedly connected to one side of the outer surface of the large piston. A cylinder is provided on the side of the slide rod away from the large piston.

[0009] Preferably, a power gear is fixedly connected to the top of the outer surface of the first rotating ring, and the outer surface of the power gear meshes with the gear plate; a first helical gear is fixedly connected to the bottom of the outer surface of the first rotating ring.

[0010] Preferably, a second helical gear is fixedly connected to the top of the outer surface of the second rotating ring, a connecting rod is rotatably embedded inside the fixed column, and both sides of the outer surface of the connecting rod are fixedly connected to transmission helical gears, and one side of the outer surface of the multiple transmission helical gears is engaged with the first helical gear, and one side of the outer surface of the second helical gear is engaged with the transmission helical gear.

[0011] Preferably, a plurality of first rotating plates are fixedly connected to the outer surface of the first rotating ring, and a first connecting post is fixedly connected to the bottom of the outer surface of each of the plurality of first rotating plates, and a first stirring plate is fixedly connected to the bottom of the outer surface of each of the plurality of first connecting posts. A plurality of second rotating plates are fixedly connected to the outer surface of the second rotating ring, and a second connecting post is fixedly connected to the bottom of the outer surface of each of the plurality of second rotating plates, and a second stirring plate is fixedly connected to the bottom of the outer surface of each of the plurality of second connecting posts.

[0012] Preferably, a connecting pipe is fixedly connected to one side of the outer surface of the measuring cylinder, and a connecting tube is provided on the outer surface of the connecting pipe. An output tube is provided on one side of the outer surface of the connecting pipe, and an electric actuator is fixedly connected to the top of the outer surface of the output tube. A small piston is fixedly connected to one side of the outer surface of the electric actuator, and the small piston is slidably embedded inside the output tube. A one-way valve is provided inside the output tube, and a liquid outlet pipe is provided inside the output tube.

[0013] Preferably, a rotary motor is fixedly connected to the top of the outer surface of the top plate, and the output shaft of the rotary motor is fixedly connected to the power rod. A storage cylinder is provided at the bottom of the outer surface of the top plate, and a connecting valve is provided inside the storage cylinder. The side of the connecting valve away from the storage cylinder is located inside the connecting cylinder. Multiple support columns are fixedly connected to the bottom of the outer surface of the top plate.

[0014] Compared with the prior art, the beneficial effects achieved by this utility model are:

[0015] When stirring is required inside the storage cylinder, this invention utilizes a rotary motor. The motor drives a power rod to rotate, which in turn drives a gear plate to slide back and forth within the rotating plate via a fixed rod. This sliding of the gear plate causes a geared power gear to rotate, which in turn rotates a first rotating ring. This first rotating ring then drives multiple first stirring plates to stir the inside of the storage cylinder. The rotation of the first rotating ring, through a first helical gear, drives multiple geared transmission helical gears to rotate, which in turn drives a second helical gear to rotate. This second helical gear then drives a second rotating ring, which in turn drives multiple second stirring plates to stir the inside of the storage cylinder. The stirring directions of the first and second connecting columns are opposite. Furthermore, the reciprocating sliding of the gear plate within the rotating plate causes the rotation direction of the power gear to constantly change. This change in the rotation direction of the power gear, in turn, causes the rotation directions of the first and second stirring plates to change accordingly. This technical solution helps maintain a uniform distribution of cells and nutrients inside the storage cylinder, preventing cell sedimentation or aggregation, and allowing for the acquisition of more representative samples during sampling.

[0016] Secondly, this utility model allows the reaction liquid to flow into the connecting cylinder by opening the connecting valve. Then, by opening the cylinder, the cylinder drives the large piston to slide inside the measuring cylinder via a slide rod, thereby drawing the reaction liquid from the connecting cylinder into the measuring cylinder. The measuring cylinder is a transparent device with graduations on the outside. A fixed amount of reaction liquid is drawn through the measuring cylinder. Subsequently, the connecting valve is closed, and the one-way valve is opened. The large and small pistons then push the reaction liquid out through the outlet pipe inside the output cylinder. This technical solution controls the volume of each sample, helping to reduce human error and ensure the accuracy and consistency of each sample. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0018] Figure 2 This is a top-view three-dimensional structural diagram of the present invention;

[0019] Figure 3 This is a three-dimensional structural diagram of the stirring assembly of this utility model;

[0020] Figure 4 This is a three-dimensional structural diagram of the disassembled stirring assembly of this utility model;

[0021] Figure 5 This is a three-dimensional structural diagram of the quantitative component of this utility model.

[0022] The components are as follows: 1. Top plate; 101. Support column; 2. Fixed column; 201. Rotating plate; 202. Gear plate; 203. Fixed rod; 204. Power rod; 205. Connecting rod; 206. Transmission helical gear; 3. First rotating ring; 301. First helical gear; 302. First rotating plate; 303. First connecting column; 304. First stirring plate; 305. Power gear; 4. Second rotating ring; 401. Second helical gear; 402. Second rotating plate; 403. Second connecting column; 404. Second stirring plate; 5. Measuring cylinder; 501. Large piston; 502. Sliding rod; 503. Cylinder; 6. Connecting cylinder; 601. Connecting pipe; 7. Output cylinder; 701. Electric actuator; 702. Small piston; 703. One-way valve; 704. Liquid outlet pipe; 8. Connecting valve; 9. Storage cylinder; 10. Rotary motor. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0024] Please see Figure 1-4 A cell reaction solution sampling device includes a top plate 1 and a measuring cylinder 5. A stirring assembly is provided at the bottom of the top plate 1, and a quantitative assembly is provided inside the measuring cylinder 5.

[0025] The stirring assembly includes a fixed column 2, which is fixedly connected to the bottom of the outer surface of the top plate 1. A first rotating ring 3 is rotatably sleeved on the outer surface of the fixed column 2, a second rotating ring 4 is rotatably sleeved on the outer surface of the fixed column 2, a rotating plate 201 is rotatably sleeved on the outer surface of the fixed column 2, and a gear plate 202 is slidably embedded inside the rotating plate 201. A power rod 204 is rotatably embedded inside the top plate 1, and a fixed rod 203 is rotatably embedded inside the power rod 204. The fixed rod 203 is rotatably embedded inside the gear plate 202.

[0026] The quantitative component includes a measuring cylinder 5, and a large piston 501 is slidably embedded inside the measuring cylinder 5. A slide rod 502 is slidably embedded inside the measuring cylinder 5. The slide rod 502 is fixedly connected to one side of the outer surface of the large piston 501. A cylinder 503 is provided on the side of the slide rod 502 away from the large piston 501.

[0027] With the above technical solution, when it is necessary to stir the inside of the storage cylinder 9, the rotary motor 10 is turned on, which drives the power rod 204 to rotate. The rotation of the power rod 204 drives the gear plate 202 to slide back and forth inside the rotating plate 201 via the fixed rod 203. The sliding of the gear plate 202 drives the geared power gear 305 to rotate. The rotation of the power gear 305 drives the first rotating ring 3 to rotate. The rotation of the first rotating ring 3 drives multiple first stirring plates 304 to stir the inside of the storage cylinder 9. The rotation of the first rotating ring 3 drives multiple geared transmission helical gears 206 to rotate via the first helical gear 301. The rotation of the transmission helical gears 206 drives the second helical gear 401 to rotate. The second helical gear 401 rotates, causing the second rotating ring 4 to rotate. The rotation of the second rotating ring 4 drives multiple second stirring plates 404 to stir the inside of the storage cylinder 9. The stirring directions of the first connecting column 303 and the second connecting column 403 are opposite. Because the gear plate 202 slides back and forth inside the rotating plate 201, the rotation direction of the power gear 305 is constantly changing. The change in the rotation direction of the power gear 305, in turn, causes the rotation directions of the first stirring plate 304 and the second stirring plate 404 to change accordingly. Through the above technical solution, the uniform distribution of cells and nutrients inside the storage cylinder 9 is maintained, preventing cell sedimentation or aggregation, so that more representative samples can be obtained during sampling.

[0028] With the above technical solution, it is assumed that the internal mixing of the storage cylinder 9 is completed. By opening the connecting valve 8, the reaction liquid flows into the interior of the connecting cylinder 6 through the connecting valve 8. By opening the cylinder 503, the cylinder 503 drives the large piston 501 to slide inside the measuring cylinder 5 through the slide rod 502, thereby drawing the reaction liquid in the connecting cylinder 6 into the measuring cylinder 5. The measuring cylinder 5 is a transparent measuring cylinder device with graduations on the outside. A certain amount of reaction liquid is drawn through the measuring cylinder 5. Then, the connecting valve 8 is closed and the one-way valve 703 is opened. The reaction liquid is pushed out from the outlet pipe 704 inside the output cylinder 7 through the large piston 501 and the small piston 702. With the above technical solution, the volume of each sample is controlled, which helps to reduce human error and ensure the accuracy and consistency of each sample.

[0029] Specifically, a power gear 305 is fixedly connected to the top of the outer surface of the first rotating ring 3, and the outer surface of the power gear 305 is toothed with the gear plate 202. A first helical gear 301 is fixedly connected to the bottom of the outer surface of the first rotating ring 3.

[0030] Through the above technical solution, the gear plate 202 slides, thereby driving the gear 305 to rotate, and the rotation of the gear 305 drives the first rotating ring 3 to rotate.

[0031] Specifically, a second helical gear 401 is fixedly connected to the top of the outer surface of the second rotating ring 4, a connecting rod 205 is rotatably embedded inside the fixed column 2, and both sides of the outer surface of the connecting rod 205 are fixedly connected to transmission helical gears 206, and one side of the outer surface of the multiple transmission helical gears 206 is engaged with the first helical gear 301, and one side of the outer surface of the second helical gear 401 is engaged with the transmission helical gear 206.

[0032] Through the above technical solution, the first rotating ring 3 rotates, which drives the multiple toothed transmission helical gears 206 to rotate via the first helical gear 301. The rotation of the transmission helical gears 206 drives the second helical gear 401 to rotate, and the rotation of the second helical gear 401 drives the second rotating ring 4 to rotate.

[0033] Specifically, a plurality of first rotating plates 302 are fixedly connected to the outer surface of the first rotating ring 3, and a first connecting post 303 is fixedly connected to the bottom of the outer surface of the plurality of first rotating plates 302, and a first stirring plate 304 is fixedly connected to the bottom of the outer surface of the plurality of first connecting posts 303. A plurality of second rotating plates 402 are fixedly connected to the outer surface of the second rotating ring 4, and a second connecting post 403 is fixedly connected to the bottom of the outer surface of the plurality of second rotating plates 402, and a second stirring plate 404 is fixedly connected to the bottom of the outer surface of the plurality of second connecting posts 403.

[0034] Through the above technical solution, the rotation of the first rotating ring 3 drives multiple first stirring plates 304 to stir the inside of the storage cylinder 9, and the rotation of the second rotating ring 4 drives multiple second stirring plates 404 to stir the inside of the storage cylinder 9.

[0035] Specifically, a connecting pipe 601 is fixedly connected to one side of the outer surface of the measuring cylinder 5, and a connecting cylinder 6 is provided on the outer surface of the connecting pipe 601. An output cylinder 7 is provided on one side of the outer surface of the connecting pipe 601, and an electric push rod 701 is fixedly connected to the top of the outer surface of the output cylinder 7. A small piston 702 is fixedly connected to one side of the outer surface of the electric push rod 701, and the small piston 702 is slidably embedded inside the output cylinder 7. A one-way valve 703 is provided inside the output cylinder 7, and a liquid outlet pipe 704 is provided inside the output cylinder 7.

[0036] Through the above technical solution, the cylinder 503 drives the large piston 501 to slide inside the measuring cylinder 5 via the slide rod 502, thereby drawing the reaction liquid in the connecting cylinder 6 into the measuring cylinder 5. The measuring cylinder 5 is a transparent measuring cylinder device with graduations on the outside. A certain amount of reaction liquid is drawn through the measuring cylinder 5. The one-way valve 703 is opened, and the reaction liquid is pushed out from the liquid outlet pipe 704 inside the output cylinder 7 through the large piston 501 and the small piston 702.

[0037] Specifically, a rotary motor 10 is fixedly connected to the top of the outer surface of the top plate 1, and the output shaft of the rotary motor 10 is fixedly connected to the power rod 204. A storage cylinder 9 is provided at the bottom of the outer surface of the top plate 1, and a connecting valve 8 is provided inside the storage cylinder 9. The side of the connecting valve 8 away from the storage cylinder 9 is located inside the connecting cylinder 6. Multiple support columns 101 are fixedly connected to the bottom of the outer surface of the top plate 1.

[0038] Through the above technical solution, the rotary motor 10 drives the power rod 204 to rotate, and the reaction liquid flows into the interior of the connecting cylinder 6 through the connecting valve 8.

[0039] In use, when it is necessary to stir the inside of the storage cylinder 9, the rotary motor 10 is turned on, which drives the power rod 204 to rotate. The rotation of the power rod 204 drives the gear plate 202 to slide back and forth inside the rotating plate 201 via the fixed rod 203. The sliding of the gear plate 202 drives the geared power gear 305 to rotate. The rotation of the power gear 305 drives the first rotating ring 3 to rotate. The rotation of the first rotating ring 3 drives multiple first stirring plates 304 to stir the inside of the storage cylinder 9. The rotation of the first helical gear 301 drives multiple geared transmission helical gears 206 to rotate, which in turn drives the second helical gear 401 to rotate. The rotation of the second helical gear 401 drives the second rotating ring 4 to rotate, which in turn drives multiple second stirring plates 404 to stir the inside of the storage cylinder 9. The stirring directions of the first connecting column 303 and the second connecting column 403 are opposite. Because the gear plate 202 slides back and forth inside the rotating plate 201, the rotation direction of the power gear 305 is constantly changing. The change in the rotation direction of the power gear 305, in turn, causes the rotation directions of the first stirring plate 304 and the second stirring plate 404 to change accordingly. Through the above technical solution, the uniform distribution of cells and nutrients inside the storage cylinder 9 is maintained, preventing cell sedimentation or aggregation, so that more representative samples can be obtained during sampling. It is assumed that the stirring inside the storage cylinder 9 has been completed. By opening the connecting valve 8, the reaction liquid flows into the inside of the connecting cylinder 6 through the connecting valve 8. By opening the cylinder 503, the cylinder 503 moves through the slide rod 50 2. The large piston 501 slides inside the measuring cylinder 5, thereby drawing the reaction liquid from the connecting cylinder 6 into the measuring cylinder 5. The measuring cylinder 5 is a transparent measuring cylinder device with graduations on the outside. A certain amount of reaction liquid is drawn through the measuring cylinder 5. Then, the connecting valve 8 is closed and the one-way valve 703 is opened. The reaction liquid is pushed out from the outlet pipe 704 inside the output cylinder 7 through the large piston 501 and the small piston 702. By using the above technical solution, the volume of each sample is controlled, which helps to reduce human error and ensure the accuracy and consistency of each sample.

[0040] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell reaction solution sampling device, comprising a top plate (1) and a graduated cylinder (5), characterized in that: A stirring assembly is provided at the bottom of the top plate (1), and a metering assembly is provided inside the measuring cylinder (5); The stirring assembly includes a fixed column (2), which is fixedly connected to the bottom of the outer surface of the top plate (1). A first rotating ring (3) is rotatably sleeved on the outer surface of the fixed column (2), and a second rotating ring (4) is rotatably sleeved on the outer surface of the fixed column (2). A rotating plate (201) is rotatably sleeved on the outer surface of the fixed column (2), and a gear plate (202) is slidably embedded inside the rotating plate (201). A power rod (204) is rotatably embedded inside the top plate (1), and a fixed rod (203) is rotatably embedded inside the power rod (204). The fixed rod (203) is rotatably embedded inside the gear plate (202). The quantitative component includes a measuring cylinder (5), and a large piston (501) is slidably embedded inside the measuring cylinder (5). A slide rod (502) is slidably embedded inside the measuring cylinder (5). The slide rod (502) is fixedly connected to one side of the outer surface of the large piston (501). A cylinder (503) is provided on the side of the slide rod (502) away from the large piston (501).

2. The cell reaction solution sampling device according to claim 1, characterized in that: A power gear (305) is fixedly connected to the top of the outer surface of the first rotating ring (3), and the outer surface of the power gear (305) is toothed with the gear plate (202). A first helical gear (301) is fixedly connected to the bottom of the outer surface of the first rotating ring (3).

3. The cell reaction solution sampling device according to claim 1, characterized in that: The top of the outer surface of the second rotating ring (4) is fixedly connected to a second helical gear (401). The inside of the fixed column (2) is rotatably embedded with a connecting rod (205). Both sides of the outer surface of the connecting rod (205) are fixedly connected to transmission helical gears (206). One side of the outer surface of the multiple transmission helical gears (206) is engaged with the first helical gear (301). One side of the outer surface of the second helical gear (401) is engaged with the transmission helical gear (206).

4. The cell reaction solution sampling device according to claim 1, characterized in that: The outer surface of the first rotating ring (3) is fixedly connected with a plurality of first rotating plates (302), and the bottom of the outer surface of the plurality of first rotating plates (302) is fixedly connected with a first connecting column (303), and the bottom of the outer surface of the plurality of first connecting columns (303) is fixedly connected with a first stirring plate (304). The outer surface of the second rotating ring (4) is fixedly connected with a plurality of second rotating plates (402), and the bottom of the outer surface of the plurality of second rotating plates (402) is fixedly connected with a second connecting column (403), and the bottom of the outer surface of the plurality of second connecting columns (403) is fixedly connected with a second stirring plate (404).

5. The cell reaction solution sampling device according to claim 1, characterized in that: A connecting pipe (601) is fixedly connected to one side of the outer surface of the measuring cylinder (5), and a connecting tube (6) is provided on the outer surface of the connecting pipe (601). An output tube (7) is provided on one side of the outer surface of the connecting pipe (601), and an electric push rod (701) is fixedly connected to the top of the outer surface of the output tube (7). A small piston (702) is fixedly connected to one side of the outer surface of the electric push rod (701), and the small piston (702) is slidably embedded in the inside of the output tube (7). A one-way valve (703) is provided inside the output tube (7), and a liquid outlet pipe (704) is provided inside the output tube (7).

6. The cell reaction solution sampling device according to claim 1, characterized in that: A rotary motor (10) is fixedly connected to the top of the outer surface of the top plate (1), and the output shaft of the rotary motor (10) is fixedly connected to the power rod (204). A storage cylinder (9) is provided at the bottom of the outer surface of the top plate (1), and a connecting valve (8) is provided inside the storage cylinder (9). The side of the connecting valve (8) away from the storage cylinder (9) is provided inside the connecting cylinder (6). A plurality of support columns (101) are fixedly connected to the bottom of the outer surface of the top plate (1).

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