Fixing system for optical imaging of living body

Abstract

The utility model provides a fixing system for optical imaging of a living body. The fixing system comprises a main body structure, a bottom cover, a cover glass, a ventilation pipeline and an imaging assembly, the main body structure comprises a base and a peripheral fence, and a containing hole is formed in the base and used for containing a living organ or tissue to be fixed; the bottom cover is in threaded connection with the base, a square groove is formed in the upper end face of the bottom cover, and a round hole communicated with the square groove is formed in the lower end face; the cover glass is arranged in the square groove, and an adsorption cavity is formed between the cover glass and the living organ or tissue between the accommodating holes; the ventilation pipeline is used for exhausting air outwards to enable the adsorption cavity to form negative pressure, living organs or tissues are fixed, and the situation that an imaging focal plane is unstable due to shaking of a living body is avoided; the imaging assembly comprises an object carrier and imaging equipment, the object carrier is used for bearing the bottom cover, and the imaging equipment is used for imaging living organs or tissues. The fixing system provided by the utility model is low in cost, wide in adaptive objective lens range, adaptive to more types of living organs or tissues, and reusable.

Description

Technical Field

[0001] This invention belongs to the field of biomedical imaging technology, and in particular relates to a fixation system for in vivo optical imaging. Background Technology

[0002] In vivo animal tissue imaging allows for real-time detection and recording of internal animal structures and physiological processes, enabling researchers to observe and analyze the structure and function of animal tissues in vivo. This technology provides a crucial tool for scientific research, allowing researchers to directly observe the occurrence and development of diseases in vivo, thereby exploring disease mechanisms. It enables real-time tracking of the dynamic changes of cells, molecules, and other biomolecules in vivo, revealing biochemical reactions and metabolic processes within organisms, understanding fundamental life activities such as cell growth, division, and apoptosis, and exploring complex life processes such as development and aging. Therefore, in vivo animal tissue imaging offers a deeper understanding of the physiological and pathological mechanisms of organisms, playing a significant role in disease diagnosis and treatment, and in-depth research into biological processes.

[0003] In biomedical research, in vivo optical imaging technology has become an important tool for observing and analyzing the structure and function of cells, tissues, and organs. However, in vivo imaging of animal tissues, tremor is a problem that requires special attention. Tremor is caused by respiration, heartbeat, or reflexes during in vivo imaging. Since in vivo animal tissue imaging is dynamic, even minute tremors can cause the imaging system to lose focus on the target tissue, resulting in blurred or distorted images. Furthermore, tremor reduces the resolution of the imaging system, making details in the image blurry or difficult to distinguish. In extreme cases, tremor may even prevent the imaging system from accurately capturing and analyzing tissue details, severely affecting the accuracy and reliability of experimental results.

[0004] When imaging live animal tissues, a series of measures are needed to reduce or eliminate the effects of vibration. For example, a stable imaging platform or support can be used to fix the imaging system, reducing vibration caused by external factors. Simultaneously, imaging equipment with high stability and sensitivity can be selected to better handle the dynamic nature of live animal tissues. Live organ fixation imaging devices can more firmly fix organs or tissues under the microscope, reducing displacement and movement of tissues under the microscope, making the observation process continuous and clear.

[0005] Traditional fixation devices have many shortcomings in terms of fixation effectiveness, applicability, and ease of operation. For example, some fixation devices are only applicable to specific types of organs or tissues and cannot flexibly meet diverse research needs; others have poor fixation effects, such as organs or tissues easily falling off, or the fixation device cannot be firmly fixed to the microscope, which can easily lead to movement and blurring during imaging; in addition, many fixation devices are cumbersome to adjust fixation conditions, affecting experimental efficiency and image quality; some fixation devices have non-adjustable adsorption pressure, resulting in inconsistent fixation effects for different organs and unsuitability for various types of tissues; for example, the adsorption fixation effect is good for kidneys, but the pressure is too high when used on lungs, which can easily damage the lungs; some fixation devices are placed above the animal, so they can only be used on upright microscopes and cannot be used on inverted microscopes. However, for life science and medical research, the frequency of use of inverted microscopes is significantly higher than that of upright microscopes. This is because the lens of an inverted microscope is below the stage, and there is more operating space on the stage, allowing direct observation of cells in culture dishes / flasks and direct scanning of the bottom of wells in multi-well plates (such as 96-well plates).

[0006] Furthermore, most live animal organ fixation devices currently on the market are made of metal, requiring advanced manufacturing processes and incurring high purchase costs. Some cannot effectively fix specific organs or tissues; others can be fixed under negative pressure, but lack a slide in the imaging window, preventing the use of high-magnification oil immersion imaging; still others have a slide in the imaging window, but it is integrally molded and embedded, rendering the entire fixation device unusable if the slide breaks. Additionally, the small window size makes it unsuitable for searching fields of interest and creating mosaics, and the inability to replace coverslips can lead to cross-contamination between different samples.

[0007] Therefore, there is a need to provide an improved technical solution that addresses the shortcomings of the existing technology.

[0008] It should be noted that the above introduction to the technical background is only for the purpose of providing a clear and complete explanation of the technical solutions of this application and facilitating understanding by those skilled in the art. It should not be assumed that these technical solutions are known to those skilled in the art simply because they have been described in the background section of this application. Utility Model Content

[0009] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide a fixation system for live optical imaging, which solves the problems of poor fixation effect, limited applicability, complex process, non-reusability and cumbersome operation of the existing live animal tissue fixation technology.

[0010] To achieve the above and other related objectives, this utility model provides a fixation system for in vivo optical imaging, the fixation system comprising:

[0011] The main structure includes a base and a fence surrounding the base. The base has a receiving hole for placing a living organ or tissue to be fixed.

[0012] The bottom cover has an internal thread on its inner wall and is threaded to the base. The upper surface of the bottom cover has a square groove, which is located directly below the receiving hole. The lower surface of the bottom cover has a circular hole that communicates with the square groove. The center point of the circular hole coincides with the center point of the square groove, and the diameter of the circular hole is smaller than the side length of the square groove.

[0013] A coverslip, wherein the coverslip is disposed in the square groove, and the coverslip and the living organ or tissue form an adsorption cavity between the receiving hole;

[0014] A ventilation tube, one end of which is connected to the receiving hole, and the other end of which is used to draw air outward to create a negative pressure in the adsorption chamber, thereby fixing the living organ or tissue.

[0015] An imaging assembly, comprising a carrier and an imaging device, wherein the carrier is a typical accessory of the imaging device and is used to support the bottom cover, and the imaging device is used to image the living organ or tissue.

[0016] Preferably, the fixing system further includes a magnetic suction unit, which includes multiple magnets and a magnetic strip. The multiple magnets are distributed and fixed on the lower end face of the bottom cover, and the magnetic strip is disposed below the shelf. The bottom cover is fixed to the shelf by the magnetic attraction between the magnets and the magnetic strip.

[0017] Preferably, the magnetic strip is annular, and the inner diameter of the magnetic strip is not less than the diameter of the circular hole.

[0018] Preferably, the fixing system further includes a negative pressure regulating device, which is connected to the ventilation pipeline via a hose. The negative pressure regulating device draws air from the adsorption chamber through the hose and the ventilation pipeline to create a negative pressure.

[0019] Preferably, a sealing ring is embedded in the lower end face of the base, the sealing ring is located on the outer ring of the receiving hole, and the sealing ring is in contact with the cover glass to form a sealed state.

[0020] Preferably, the side of the sealing ring adjacent to the bottom cover protrudes from the lower end face of the base.

[0021] Preferably, the diameter of the circular hole is not less than the inner diameter of the receiving hole on the side adjacent to the cover glass.

[0022] Preferably, the ventilation pipe is inclined, with one end penetrating the sidewall of the receiving hole and the other end penetrating the fence and leading to the outside of the main structure.

[0023] Preferably, the main structure and the ventilation pipe are integrally formed using 3D printing technology.

[0024] Preferably, the bottom cover is made using a 3D printing process.

[0025] As described above, the fixation system for live optical imaging of this invention has the following beneficial effects:

[0026] The fixation system of this invention is low in cost, has a wide range of compatible objectives, is suitable for a greater variety of living organs or tissues, and is reusable. The fixation system includes a main structure, a base cover, a coverslip, a ventilation tube, and an imaging assembly. The base cover is threadedly connected to the base of the main structure. The coverslip is located in a square groove in the base cover, forming an adsorption cavity between the living organ or tissue and the coverslip. When air is drawn out through the ventilation tube, a negative pressure is created in the adsorption cavity, causing the living organ or tissue to be attracted and adhered to the coverslip, thus fixing the living organ or tissue and preventing imaging interference caused by the organ's own vibration. The focal plane is unstable; and the negative pressure of the adsorption chamber is controlled by adjusting the air pressure of the negative pressure regulating device, thus adapting to different types of living organs or tissues. The adsorption force is adjusted according to the different tolerances of the living organisms, without causing damage to the living organisms; multiple magnets are set on the lower end face of the bottom cover, and the magnetic strip is placed under the carrier. The magnetic attraction between the magnets and the magnetic strip fixes the bottom cover to the carrier, which can firmly fix the bottom cover and make it difficult for it to move. This fixes the position of the entire living organism, thus truly achieving absolute fixation of the living organism's position, achieving focal plane stability of the imaging, and realizing high-quality optical imaging of living organs or tissues.

[0027] The main structure and ventilation pipeline of this utility model are integrally formed using 3D printing technology. The bottom cover is also 3D printed, achieving a fine structure in a small volume. The process is simple and the cost is low. The cover glass can be replaced at any time, and the damage to the entire fixing device will not be caused by the breakage of the cover glass. Replacing the cover glass with a new one between each sample can avoid cross-contamination between samples. Attached Figure Description

[0028] Figure 1 The diagram shown is a first-view three-dimensional structural schematic of the main structure and ventilation pipeline in a specific embodiment of this utility model.

[0029] Figure 2The diagram shown is a three-dimensional structural schematic of the main structure and ventilation pipeline from another perspective in a specific embodiment of this utility model.

[0030] Figure 3 The diagram shown is a first-view perspective three-dimensional structural diagram of the bottom cover in a specific embodiment of this utility model.

[0031] Figure 4 The diagram shown is a three-dimensional structural schematic of the bottom cover from another perspective in a specific embodiment of this utility model.

[0032] Figure 5 The diagram shown is a partial structural schematic of the fixing system in a specific embodiment of this utility model.

[0033] Figure 6 The diagram shows the positional relationship between the bottom cover and the shelf in a specific embodiment of this utility model.

[0034] Figure 7 The diagram shown is a structural schematic of the fixing system in a specific embodiment of this utility model.

[0035] Component designation explanation

[0036] 10. Main Structure

[0037] 101 Base

[0038] 102 Fence

[0039] 103 Accommodating holes

[0040] 104 Sealing Ring

[0041] 105 Adsorption Chamber

[0042] 106. Living organs or tissues

[0043] 20 Bottom Cover

[0044] 201 Square Groove

[0045] 202 round hole

[0046] 203 Coverslip

[0047] 30 Ventilation tubing

[0048] 401 Shelf

[0049] 402 Imaging Equipment Lens

[0050] 501 magnet

[0051] 502 magnetic stripe

[0052] 60 Hose

[0053] 70 Negative pressure regulating device Detailed Implementation

[0054] The following description, in conjunction with the accompanying drawings of the embodiments of this application, outlines various embodiments of this application. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This application can also be implemented or applied through other different specific embodiments, and various details in this specification can be modified or changed based on different viewpoints and applications without departing from the spirit of this application. For ease of explanation, when detailing the embodiments of this application, the cross-sectional views illustrating the device structure may be partially enlarged without adhering to the general scale, and the schematic diagrams are merely examples and should not limit the scope of protection of this application. Furthermore, in actual manufacturing, the three-dimensional spatial dimensions of length, width, and depth should be included.

[0055] In the description of the embodiments of this application, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, "connection" can be a detachable connection or a non-detachable connection; it can be a direct connection or an indirect connection through an intermediate medium. "Fixed connection" refers to a connection where the relative positional relationship remains unchanged after connection. It should be understood that when component A is fixedly connected to component C via component B, changes in the relative positional relationship due to deformation of components A, B, and C are permissible. "Rotary connection" refers to a connection where the components can rotate relative to each other after connection. "Sliding connection" refers to a connection where the components can slide relative to each other after connection. The phrase "two components forming an integrated structure through a one-piece molding process" means that during the formation of one of the two components, that component is connected to the other component, without requiring further processing (such as bonding, welding, snap-fit ​​connections, or screw connections) to connect the two components.

[0056] The directional terms mentioned in the embodiments of this application, such as "upper", "lower", "side", "top", "bottom", etc., are only for reference to the direction of the accompanying drawings. Therefore, the directional terms used are for better and clearer explanation and understanding of the embodiments of this application, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.

[0057] The term "multiple" refers to at least two. The term "more than" includes the stated number. The term "and / or" describes a relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A alone, A and B simultaneously, or B alone. The terms "first," "second," etc., are used for descriptive purposes only and should not be construed as implying or suggesting relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include one or more of that feature.

[0058] Please refer to the following: Figures 1-7 This utility model provides a fixation system for live optical imaging, which includes a main structure 10, a bottom cover 20, a cover glass 203, a ventilation tube 30, and an imaging component.

[0059] The main structure 10 includes a base 101 and a fence 102 disposed around the base 101. The base 101 has a receiving hole 103 for placing a living organ or tissue 106 to be fixed.

[0060] The inner wall of the bottom cover 20 is provided with internal threads, and the bottom cover 20 is threadedly connected to the base 101. A square groove 201 is provided on the upper end face of the bottom cover 20, and the square groove 201 is correspondingly provided directly below the receiving hole 103. A circular hole 202 is provided on the lower end face of the bottom cover 20, which is interconnected with the square groove 201. The center point of the circular hole 202 coincides with the center point of the square groove 201, and the diameter of the circular hole 202 is smaller than the side length of the square groove 201.

[0061] A cover glass 203 is disposed in the square groove 201, and the cover glass 203 and the living organ or tissue 106 form an adsorption cavity 105 between the receiving hole 103;

[0062] One end of the ventilation pipe 30 is connected to the receiving hole 103, and the other end of the ventilation pipe 30 is used to draw air outward to create a negative pressure in the adsorption chamber 105, thereby fixing the living organ or tissue 106.

[0063] The imaging assembly includes a carrier 401 and an imaging device 402. The carrier 401 is used to support the bottom cover 20, and the imaging device 402 is used to image the living organ or tissue 106.

[0064] In a specific embodiment of this utility model, the base 101 is disc-shaped, with a thickness of 2 mm and a diameter of 31 mm. The bottom cover 20 has a diameter of 35 mm, which is the same as the diameter of the confocal culture dish, so as to be compatible with various microscopes in the prior art.

[0065] In a specific embodiment of this utility model, see [reference]. Figure 1 , 2 and Figure 5 The receiving hole 103 includes three holes of different sizes: an upper hole, a middle hole, and a lower hole. The upper hole is conical in shape, with the largest diameter at the top. The middle hole is a circular hole 202, with the same diameter as the lowest end of the upper hole. The diameter of the lower hole is larger than that of the middle hole. In this case, the diameter of the middle hole is 4mm to 18mm, and the appropriate diameter is selected according to the size of the living organ or tissue 106. Of course, in other specific embodiments, the receiving hole 103 can also be other shapes, and no further restrictions are imposed here.

[0066] Specifically, the base 101 has a receiving hole 103 for placing a living organ or tissue 106 to be fixed. The inner wall of the receiving hole 103 needs to be smooth to avoid damage to the living organ. The side wall of the base 101 is provided with external threads, and the bottom cover 20 is provided with internal threads. The bottom cover 20 is threadedly connected to the base 101. When the living organ or tissue 106 is placed in the receiving hole 103, a sealed space is formed between the living organ or tissue 106 and the coverslip 203, which is the adsorption chamber 105. Since the ventilation pipe 30 is connected to the receiving hole 103, when the ventilation pipe 30 draws air outward, a negative pressure state is formed in the adsorption chamber 105. At this time, the living organ or tissue 106 will be attracted and attached to the coverslip 203, thereby fixing the living organ or tissue 106 and avoiding instability of the imaging focal plane due to the shaking of the living organ itself, thus assisting in the optical imaging of the living organ.

[0067] Specifically, the coverslip 203 is a square, conventional coverslip with a side length slightly smaller than that of the square groove 201, and its thickness is not less than that of the groove. Furthermore, the coverslip 203 is replaceable at any time; it can be replaced simply by unscrewing the thread between the bottom cover 20 and the base 101. Damage to the coverslip 203 will not damage the entire mounting device, thus achieving reusability. Moreover, a new coverslip 203 can be replaced after each sample is imaged, avoiding the risk of cross-contamination between samples. Additionally, the coverslip 203 provides the capability for imaging with various lenses, not only for air microscopes but also for water microscopes and oil microscopes, broadening its applicability.

[0068] In addition, during optical imaging, the imaging device includes the imaging device lens 402 and other components, which will not be elaborated on here; please refer to [reference needed]. Figure 7 The imaging device lens 402 is positioned below the carrier 401 and is used to image living organs or tissues 106. In practical applications, the carrier 401 is an accessory of the imaging device itself.

[0069] In a specific embodiment of this utility model, the square groove 201 has a side length of 22.5 mm and a thickness of 0.13 mm; the cover glass 203 is a square conventional cover glass with a side length of 22 mm and a thickness of 0.13 to 0.17 mm.

[0070] As an example, the fixing system also includes a magnetic suction unit, which includes a plurality of magnets 501 and a magnetic strip 502. The plurality of magnets 501 are distributed and fixed on the lower end face of the bottom cover 20, and the magnetic strip 502 is disposed below the shelf 401. The bottom cover 20 is fixed to the shelf 401 by the magnetic attraction between the magnets 501 and the magnetic strip 502.

[0071] For details, please refer to Figure 6 This is a schematic diagram showing the positional relationship between the bottom cover 20 and the carrier 401. Multiple magnets 501 are evenly distributed on the lower end face of the bottom cover 20. In conjunction with the magnetic strip 502, the bottom cover 20 can be fixed on the carrier 401. At this time, the bottom cover 20 can be firmly fixed, making it difficult for it to move. This fixes the position of the entire living body, preventing changes in its Z-axis position due to contact with the lens during imaging, and preventing changes or shifts in its horizontal position due to other traction or pulling. This truly achieves absolute fixation of the position of the living organ or tissue 106, which can effectively assist in living optical imaging, ensure the stability of the focal plane in real-time imaging, and achieve high-quality living optical imaging.

[0072] Preferably, see Figure 4 Four magnets 501 are provided, and the four magnets 501 are evenly installed circumferentially on the lower end face of the bottom cover 20. Of course, in other specific embodiments, more magnets 501 can be provided, and no excessive limitation is made here.

[0073] As an example, the magnetic strip 502 is annular, and the inner diameter of the magnetic strip 502 is not less than the diameter of the circular hole 202. Of course, in other specific embodiments, the magnetic strip 502 may also be of other shapes, and no excessive restrictions are imposed here.

[0074] Specifically, the magnetic strip 502 is a magnetic material used to enhance or guide the direction of a magnetic field. It is typically made of soft magnetic material and can concentrate and guide a magnetic field to a specific area. No further restrictions are placed on the material of the magnetic strip 502 here; see [reference needed]. Figure 6 The magnetic strip 502 is set in a ring shape, and the inner diameter of the middle is not less than the diameter of the circular hole 202. The circular hole 202 is the observation hole, so as not to affect the optical imaging.

[0075] As an example, the fixing system also includes a negative pressure regulating device 70, which is connected to the ventilation pipe 30 via a hose 60. The negative pressure regulating device 70 draws air from the adsorption chamber 105 through the hose 60 and the ventilation pipe 30 to create a negative pressure.

[0076] For details, please refer to Figure 7 One end of the flexible tube 60 is connected to the ventilation tube 30, and the other end is connected to the negative pressure regulating device 70. The negative pressure regulating device 70 draws air away from the flexible tube 60 and the adsorption chamber 105, creating a negative pressure inside the adsorption chamber 105, thereby adsorbing the living organ or tissue 106. Furthermore, the air pressure of the negative pressure regulating device 70 is adjustable, controlling the negative pressure inside the adsorption chamber 105. This allows the fixation system of this invention to be adapted to many types of living organs or tissues 106, and different pressure levels can be set according to the pressure tolerance of different living organisms to ensure that the living organism is not damaged. This allows for long-term, stable observation while maintaining the activity of the living organ or tissue 106, and is suitable for various living organs or tissues 106, such as the heart, liver, skin, lungs, and muscles of rats and mice.

[0077] In a specific embodiment of this utility model, the length of the hose 60 is 0.5m to 1m. The shorter the hose 60, the less lag there is in adjusting the negative pressure.

[0078] As an example, a sealing ring 104 is embedded on the lower end face of the base 101. The sealing ring 104 is located on the outer ring of the receiving hole 103, and the sealing ring 104 contacts the cover glass 203 to form a sealed state.

[0079] As an example, the sealing ring 104 is adjacent to one side of the bottom cover 20 and protrudes from the lower end face of the base 101.

[0080] For details, please refer to Figure 2 The sealing ring 104 is embedded in the lower end face of the base 101 and surrounds the periphery of the receiving hole 103; see reference Figure 5 The sealing ring 104 protrudes from the lower end face of the base 101. When the base and the bottom cover 20 are tightened, the protruding part of the sealing ring 104 contacts the cover glass 203 to form a seal. Preferably, the sealing ring 104 is made of silicone strip material.

[0081] As an example, the diameter of the circular hole 202 is not less than the inner diameter of the receiving hole 103 on the side adjacent to the cover glass 203.

[0082] For details, please refer to Figure 3The circular hole 202 serves as an observation hole. The inner diameter of the circular hole 202 is smaller than the side length of the square groove 201, and is at least larger than the inner diameter of the side of the receiving hole 103 adjacent to the cover glass 203, so as to allow the lens to observe the sample.

[0083] As an example, the ventilation pipe 30 is inclined, with one end of the ventilation pipe 30 penetrating the side wall of the receiving hole 103 and the other end penetrating the fence 102 and leading to the outside of the main structure 10.

[0084] For details, please refer to Figure 1 , Figure 2 and Figure 5 The receiving hole 103 includes three holes of different sizes: an upper hole, a middle hole, and a lower hole. The upper hole is cone-shaped, and the middle and lower holes are stepped holes. A vent is provided on the side wall at the connection between the middle and lower holes. The vent is connected to one end of the ventilation pipe 30, so that when the ventilation pipe 30 draws air outward, the vent will not be blocked by the adsorption between the living organ or tissue 106 and the side wall of the receiving hole 103.

[0085] As an example, the main structure 10 and the ventilation pipe 30 are integrally formed using 3D printing technology.

[0086] As an example, the bottom cover 20 is made using a 3D printing process.

[0087] Specifically, the main structure 10 and the ventilation pipe 30 are integrally formed. The main structure 10, the ventilation pipe 30, and the bottom cover 20 are all made using 3D printing technology. The contact area with the living body is flat and smooth, and will not cause damage to the living body. Moreover, 3D printing technology can achieve fine structural production in a small volume. It is small in size and light in weight, and will not cause damage to the imaging device 402 due to excessive weight. It also achieves a precision that traditional metal processing cannot achieve, which is convenient to implement and has a low cost.

[0088] In summary, the fixation system of this invention is low in cost, has a wide range of compatible objectives, is suitable for a greater variety of living organs or tissues, and is reusable. The fixation system includes a main structure, a base cover, a coverslip, a ventilation tube, and an imaging component. The base cover is threadedly connected to the base of the main structure. The coverslip is located in a square groove in the base cover, forming an adsorption cavity between the living organ or tissue and the coverslip. When air is drawn out through the ventilation tube, a negative pressure is created in the adsorption cavity, causing the living organ or tissue to be attracted and adhered to the coverslip, thus fixing the living organ or tissue and preventing injury due to the organ's own shaking. The imaging focal plane is unstable; however, the negative pressure of the adsorption chamber is controlled by adjusting the air pressure of the negative pressure regulating device, thus adapting to different types of living organs or tissues. The adsorption force is adjusted according to the different tolerances of the living organisms, causing no damage. Multiple magnets are placed on the lower end face of the bottom cover, and magnetic strips are placed below the carrier. The magnetic attraction between the magnets and the magnetic strips fixes the bottom cover to the carrier, firmly securing it and preventing movement. This, in turn, fixes the entire living organism's position, achieving absolute fixation and stable imaging focal plane, resulting in high-quality optical imaging of living organs or tissues. The main structure and ventilation pipeline of this invention are integrally formed using 3D printing technology, and the bottom cover is also 3D printed, achieving a refined structure within a small volume. The process is simple and inexpensive. The coverslip can be replaced at any time, preventing damage to the entire fixation device due to coverlip breakage. Replacing the coverslip with a new one between samples avoids cross-contamination. Therefore, this utility model effectively overcomes the various shortcomings of the prior art and has high industrial application value.

[0089] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Where there is no conflict, the embodiments and features in the embodiments of this application can be combined with each other. The structural materials, dimensions, shapes, etc., mentioned in the embodiments of this application are all illustrative descriptions and do not constitute strict or absolute limitations. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A fixation system for in vivo optical imaging, characterized in that, The fixed system includes: The main structure includes a base and a fence surrounding the base. The base has a receiving hole for placing a living organ or tissue to be fixed. The bottom cover has an internal thread on its inner wall and is threaded to the base. The upper surface of the bottom cover has a square groove, which is located directly below the receiving hole. The lower surface of the bottom cover has a circular hole that communicates with the square groove. The center point of the circular hole coincides with the center point of the square groove, and the diameter of the circular hole is smaller than the side length of the square groove. A coverslip, wherein the coverslip is disposed in the square groove, and the coverslip and the living organ or tissue form an adsorption cavity between the receiving hole; A ventilation tube, one end of which is connected to the receiving hole, and the other end of which is used to draw air outward to create a negative pressure in the adsorption chamber, thereby fixing the living organ or tissue. An imaging assembly, comprising a carrier and an imaging device, wherein the carrier is used to support the bottom cover and the imaging device is used to image the living organ or tissue.

2. The fixation system for live optical imaging according to claim 1, characterized in that: The fixing system also includes a magnetic suction unit, which includes multiple magnets and magnetic strips. The multiple magnets are distributed and fixed on the lower end face of the bottom cover, and the magnetic strips are disposed below the shelf. The bottom cover is fixed to the shelf by the magnetic attraction between the magnets and the magnetic strips.

3. The fixation system for live optical imaging according to claim 2, characterized in that: The magnetic strip is circular, and the inner diameter of the magnetic strip is not less than the diameter of the circular hole.

4. The fixation system for live optical imaging according to claim 1, characterized in that: The fixing system also includes a negative pressure regulating device, which is connected to the ventilation pipeline via a hose. The negative pressure regulating device draws air from the adsorption chamber through the hose and the ventilation pipeline to create a negative pressure.

5. The fixation system for live optical imaging according to claim 1, characterized in that: A sealing ring is embedded in the lower end face of the base. The sealing ring is located on the outer ring of the receiving hole, and the sealing ring is in contact with the cover glass to form a sealed state.

6. The fixation system for live optical imaging according to claim 5, characterized in that: The sealing ring protrudes from the lower end face of the base on the side adjacent to the bottom cover.

7. The fixation system for live optical imaging according to claim 1, characterized in that: The diameter of the circular hole is not less than the inner diameter of the side of the receiving hole adjacent to the cover glass.

8. The fixation system for live optical imaging according to claim 1, characterized in that: The ventilation pipe is inclined, with one end penetrating the side wall of the receiving hole and the other end penetrating the fence and leading to the outside of the main structure.

9. The fixation system for live optical imaging according to claim 8, characterized in that: The main structure and the ventilation pipeline are integrally formed using 3D printing technology.

10. The fixation system for live optical imaging according to claim 1, characterized in that: The bottom cover is made using 3D printing technology.

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