Porous nucleic acid mass spectrum adsorption resin sample adding device

Abstract

The utility model discloses a multi-hole site nucleic acid mass spectrum adsorbent resin sample adding device, including: shell subassembly and adjusting subassembly, shell subassembly includes adjusting cylinder, adjusting hole, spring, row box, top hole, chute, straw and finger groove, the circumference surface of adjusting cylinder is provided with adjusting hole, the inside of adjusting cylinder is provided with spring, row box, top hole, chute, straw and finger groove, adjusting cylinder is provided with spring, row box, top hole, chute, straw and finger groove. A row box is fixedly connected to the bottom of the adjusting cylinder, a top hole is formed in the top of the row box, and a sliding groove is formed in the inner wall of the row box. By arranging the shell assembly and the adjusting assembly, when resin adsorption is carried out, a pressing plate can be pressed to a specified scale, a piston head moves downwards in a suction pipe under the action of a movable plate and a connecting rod, then the suction pipe is placed in a resin solution, after the pressing plate is loosened, resin enters the suction pipe, and when the resin is injected, the pressing plate is pressed again; quantitative resin is extruded by observing the graduated scale, so that accurate feeding of ion exchange resin particles into a plurality of PCR tubes at the same time can be realized, and errors among different reaction tubes are reduced.

Description

Technical Field

[0001] This utility model relates to the field of sample loading device technology, specifically a multi-porous nucleic acid mass spectrometry adsorption resin sample loading device. Background Technology

[0002] During nucleic acid mass spectrometry experiments, it is essential to minimize the impurity content in the sample. A common purification method is to add ion-exchange resin particles to the PCR tube after the PCR reaction, mix well, and then centrifuge. However, the resin particles are spherical, with a diameter of approximately 0.1–0.2 mm, and exhibit some viscosity, making precise extraction, transfer, and insertion difficult.

[0003] In existing technologies, the resin particle loading process does not fully consider the viscous properties of the resin particles themselves. Only a stainless steel spoon is used for scooping and transferring, which often results in inconsistent loading amounts in multiple wells. This affects the consistency of purification effects among multiple samples and ultimately affects the reliability of experimental results. Therefore, a multi-well nucleic acid mass spectrometry adsorption resin loading device is needed. Utility Model Content

[0004] The purpose of this invention is to provide a porous nucleic acid mass spectrometry adsorption resin loading device. During resin adsorption, the pressure plate can be pressed to the specified scale, and the piston head moves down in the pipette under the action of the moving plate and connecting rod. Then, the pipette is placed in the resin solution. After releasing the pressure plate, the resin enters the pipette. When injecting the resin, the pressure plate is pressed again, and the scale is observed to squeeze out a quantitative amount of resin. This enables the precise loading of ion exchange resin particles into multiple PCR tubes simultaneously, reducing the error between different reaction tubes.

[0005] To achieve the above objectives, a porous nucleic acid mass spectrometry adsorption resin sample loading device is provided, comprising: a shell assembly and an adjustment assembly. The shell assembly includes an adjustment cylinder, an adjustment hole, a spring, a sorting box, a top hole, a sliding groove, a pipette, and a finger groove. An adjustment hole is provided on the circumferential surface of the adjustment cylinder. A spring is disposed inside the adjustment cylinder. A sorting box is fixedly connected to the bottom of the adjustment cylinder. A top hole is provided on the top of the sorting box. Four sliding grooves are provided on the inner wall of the sorting box, symmetrically distributed on the inner four sides of the sorting box. Eight pipettes are fixedly connected inside the sorting box, evenly distributed at the bottom of the sorting box. Four finger grooves are provided on the circumferential surface of the adjustment cylinder. The components are evenly distributed on the adjusting cylinder, and a scale is provided on the circumferential surface of the adjusting cylinder. The scale is located on the side of the adjusting hole. The adjusting assembly includes a moving plate, a pressure plate, an upper sealing block, a lower sealing block, a connecting rod, a box plate, sliders, and piston heads. The pressure plate is fixedly connected to the circumferential surface of the moving plate. The upper sealing block is fixedly connected to the top of the moving plate. The lower sealing block is fixedly connected to the bottom of the moving plate. The connecting rod is fixedly connected to the bottom of the moving plate. The box plate is fixedly connected to the bottom of the connecting rod. Sliders are fixedly connected to the four side walls of the box plate. There are four sliders, which are symmetrically distributed on the box plate. Piston heads are fixedly connected to the bottom of the box plate. There are eight piston heads, which are evenly distributed on the box plate.

[0006] According to the aforementioned porous nucleic acid mass spectrometry adsorption resin sample loading device, the moving plate is located inside the regulating cylinder, and the pressure plate is located inside the regulating hole.

[0007] According to the aforementioned porous nucleic acid mass spectrometry adsorption resin sample loading device, both the upper and lower sealing blocks are arc-shaped and fit against the inner wall of the regulating cylinder.

[0008] According to the aforementioned porous nucleic acid mass spectrometry adsorption resin sample loading device, the top of the spring is fixedly connected to the bottom of the moving plate, the bottom of the spring is fixedly connected to the top of the packing box, and the connecting rod is located inside the spring.

[0009] According to the aforementioned porous nucleic acid mass spectrometry adsorption resin sample loading device, the bottom of the connecting rod penetrates through the top hole and is fixedly connected to the box plate.

[0010] According to the aforementioned porous nucleic acid mass spectrometry adsorption resin sample loading device, the slider is located inside the trough, and the piston head is located inside the pipette.

[0011] According to the aforementioned porous nucleic acid mass spectrometry adsorption resin sample loading device, the bottom of the pipette penetrates through the packing box.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows: by setting the outer shell assembly and the adjustment assembly, when resin adsorption is performed, the pressure plate can be pressed to the specified scale, and the piston head moves down in the pipette under the action of the moving plate and the connecting rod. Then the pipette is placed in the resin solution. After the pressure plate is released, the resin enters the pipette. When injecting resin, the pressure plate is pressed again, and the scale is observed to squeeze out a quantitative amount of resin. This enables the precise addition of ion exchange resin particles to multiple PCR tubes at the same time, reducing the error between different reaction tubes.

[0013] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0015] Figure 1 This is a front view of a porous nucleic acid mass spectrometry adsorption resin sample loading device according to the present invention;

[0016] Figure 2 This is a cross-sectional view of the outer shell assembly of a porous nucleic acid mass spectrometry adsorption resin sample loading device according to the present invention;

[0017] Figure 3 This is a structural diagram of the adjustment component of a porous nucleic acid mass spectrometry adsorption resin sample loading device according to the present invention;

[0018] Figure 4 This is a cross-sectional view of a porous nucleic acid mass spectrometry adsorption resin sample loading device according to the present invention.

[0019] In the diagram: 1. Outer shell assembly; 2. Adjustment assembly; 101. Adjustment cylinder; 102. Adjustment hole; 103. Spring; 104. Arrangement box; 105. Top hole; 106. Slide groove; 107. Straw; 108. Finger groove; 201. Moving plate; 202. Pressure plate; 203. Upper sealing block; 204. Lower sealing block; 205. Connecting rod; 206. Box plate; 207. Slider; 208. Piston head. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figures 1-4This utility model provides a technical solution: a porous nucleic acid mass spectrometry adsorption resin sample loading device, comprising: a shell assembly 1 and an adjustment assembly 2. The shell assembly 1 includes an adjustment cylinder 101, an adjustment hole 102, a spring 103, a packing box 104, a top hole 105, a slide groove 106, a pipette 107, and a finger groove 108. The adjustment hole 102 is provided on the circumferential surface of the adjustment cylinder 101, and the spring 103 is provided inside the adjustment cylinder 101. The packing box 104 is fixedly connected to the bottom of the adjustment cylinder 101, the top hole 105 is provided on the top of the packing box 104, and the slide groove 106 is provided on the inner wall of the packing box 104. There are four grooves 106, which are symmetrically distributed on the inner four sides of the discharge box 104. The discharge box 104 is fixedly connected to the inside of the discharge box 104. There are eight straws 107, which are evenly distributed on the bottom of the discharge box 104. The bottom of the straws 107 penetrates the discharge box 104. There are four finger grooves 108 on the circumferential surface of the adjusting cylinder 101, which are evenly distributed on the upper and lower parts of the adjusting cylinder 101. A scale is provided on the circumferential surface of the adjusting cylinder 101. The scale is located on the side of the adjusting hole 102 and is used for quantitative inhalation and quantitative discharge.

[0022] The adjusting assembly 2 includes a movable plate 201, a pressure plate 202, an upper sealing block 203, a lower sealing block 204, a connecting rod 205, a box plate 206, a slider 207, and a piston head 208. The pressure plate 202 is fixedly connected to the circumferential surface of the movable plate 201. The upper sealing block 203 is fixedly connected to the top of the movable plate 201. The lower sealing block 204 is fixedly connected to the bottom of the movable plate 201. The connecting rod 205 is fixedly connected to the bottom of the connecting rod 205. The box plate 206 is fixedly connected to the four side walls of the box plate 206. There are four sliders 207, which are symmetrically distributed on the box plate 206. There are eight piston heads 208, which are evenly distributed on the box plate 206.

[0023] The movable plate 201 is located inside the adjusting cylinder 101, the pressure plate 202 is located inside the adjusting hole 102, the upper sealing block 203 and the lower sealing block 204 are both arc-shaped and fit against the inner wall of the adjusting cylinder 101 to seal the adjusting hole 102, the top of the spring 103 is fixedly connected to the bottom of the movable plate 201, the bottom of the spring 103 is fixedly connected to the top of the discharge box 104, and the connecting rod 205 is located inside the spring 103. When the spring 103 deforms, the movable plate can be moved. The vertical movement of 201 and connecting rod 205, with the bottom of connecting rod 205 passing through top hole 105 and fixedly connected to box plate 206, slider 207 located inside slide groove 106, allows the lower box plate 206 to move vertically along slide groove 106 when connecting rod 205 moves vertically, piston head 208 located inside suction tube 107, allows piston head 208 to move vertically inside suction tube 107 when box plate 206 moves vertically, thereby enabling the suction and discharge of resin.

[0024] Working principle: When adsorbing resin, press the pressure plate 202, the moving plate 201 slides down the adjusting cylinder 101, and at the same time, the spring 103 is compressed and deformed. The connecting rod 205 pushes the box plate 206 down along the slide groove 106. During the downward movement, the piston head 208 moves down the pipette 107, inserting the bottom of the pipette 107 into the resin sample. Then, release the pressure plate 202. The moving plate 201, connecting rod 205, box plate 206 and piston head 208 move up under the action of the spring 103, thereby drawing the resin into each pipette 107. When injecting, press the pressure plate 202 again. Observe the scale on the adjusting cylinder 101 and press the pressure plate multiple times to dispense the resin from each pipette 107. Eight samples can be injected at one time. It should be noted that the pressure plate 202 should not be released during this process, and the pipette 107 should not be emptied directly after the last sample is dispensed. The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.

Claims

1. A porous nucleic acid mass spectrometry adsorption resin sample loading device, characterized in that, include: The housing assembly (1) and the adjustment assembly (2) are provided. The housing assembly (1) includes an adjustment cylinder (101), an adjustment hole (102), a spring (103), a chute (104), a top hole (105), a slide (106), a straw (107), and a finger groove (108). The adjustment cylinder (101) has an adjustment hole (102) on its circumferential surface. The adjustment cylinder (101) has a spring (103) inside it. The bottom of the adjustment cylinder (101) is fixedly connected to the chute (104), and the top of the chute (107) has a top hole (108). 5) The inner wall of the tray (104) is provided with a sliding groove (106). There are four sliding grooves (106) and they are symmetrically distributed on the inner four sides of the tray (104). A straw (107) is fixedly connected inside the tray (104). There are eight straws (107) and they are evenly distributed at the bottom of the tray (104). The circumferential surface of the adjusting cylinder (101) is provided with a finger groove (108). There are four finger grooves (108) and they are evenly distributed on the upper and lower surfaces of the adjusting cylinder (101). The adjusting cylinder (101) has a scale on its circumferential surface, which is located on the side of the adjusting hole (102). The adjusting assembly (2) includes a moving plate (201), a pressure plate (202), an upper sealing block (203), a lower sealing block (204), a connecting rod (205), a box plate (206), a slider (207), and a piston head (208). The pressure plate (202) is fixedly connected to the circumferential surface of the moving plate (201), and the upper sealing block (203) is fixedly connected to the top of the moving plate (201). The bottom of the moving plate (201) has a scale on its circumferential surface, which is located on the side of the adjusting hole (102). A lower sealing block (204) is fixedly connected to the bottom of the movable plate (201). A connecting rod (205) is fixedly connected to the bottom of the connecting rod (205). A box plate (206) is fixedly connected to the bottom of the connecting rod (205). Slider blocks (207) are fixedly connected to the four side walls of the box plate (206). There are four sliders (207) and they are symmetrically distributed on the box plate (206). A piston head (208) is fixedly connected to the bottom of the box plate (206). There are eight piston heads (208) and they are evenly distributed on the box plate (206).

2. The porous nucleic acid mass spectrometry adsorption resin sample loading device as described in claim 1, characterized in that: The movable plate (201) is located inside the adjusting cylinder (101), and the pressure plate (202) is located inside the adjusting hole (102).

3. The porous nucleic acid mass spectrometry adsorption resin sample loading device as described in claim 1, characterized in that: Both the upper sealing block (203) and the lower sealing block (204) are arc-shaped and fit against the inner wall of the adjusting cylinder (101).

4. The porous nucleic acid mass spectrometry adsorption resin sample loading device as described in claim 1, characterized in that: The top of the spring (103) is fixedly connected to the bottom of the movable plate (201), the bottom of the spring (103) is fixedly connected to the top of the box (104), and the connecting rod (205) is located inside the spring (103).

5. The porous nucleic acid mass spectrometry adsorption resin sample loading device as described in claim 1, characterized in that: The bottom of the connecting rod (205) passes through the top hole (105) and is fixedly connected to the box plate (206).

6. The porous nucleic acid mass spectrometry adsorption resin sample loading device as described in claim 1, characterized in that: The slider (207) is located inside the groove (106), and the piston head (208) is located inside the straw (107).

7. The porous nucleic acid mass spectrometry adsorption resin sample loading device as described in claim 1, characterized in that: The bottom of the straw (107) passes through the tray (104).

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