Shake flask for batch production of 3D cell derivatives

Abstract

The utility model provides a 3D cell derivative batch production shake flask which comprises a base and a main body, and the outer diameter of the base is larger than that of the main body; the main body is vertically arranged, and the lower end of the main body is inserted into the cavity of the base; a plurality of protruding rings are arranged at the upper end of the body, two ends of the body are of open structures, a boss protruding upwards is arranged on a bottom plate of the base, a plurality of functional grooves are formed in one end, extending into an inner cavity of the base, of the body, the diameter of the lower portion of the base is smaller than that of the upper portion of the base, the cross section of the upper portion of the base is of an arc-shaped structure, and the upper portion and the lower portion of the base are in fillet transition. The device is reasonable in structural design, the base is arranged on the lower side of the main body, the diameter of the base is larger than that of the main body, the lower end of the main body extends into the inner cavity of the base, the lower end of the main body plays a stirring role, operation is easy, solution mixing efficiency is high, solution mixing uniformity is reliably guaranteed, and batch production of 3D cell derivatives is better facilitated.

Description

Shake flasks for mass production of 3D cell derivatives Technical Field

[0001] This invention belongs to the field of cell culture technology, and in particular relates to a shake flask for mass production of 3D cell derivatives. Background Technology

[0002] Large-scale bacterial / cell culture technology refers to the technique of cultivating bacteria / cells at high density in a bacterial / cell bioreactor under artificially controlled conditions, including pH, temperature, and dissolved oxygen. These products are used to produce a wide range of biological products, such as bread, soy sauce, yogurt, vaccines, antibiotics, and antibodies. Traditional bacterial / cell culture techniques in laboratory research utilize shake flasks for large-scale bacterial / cell culture. Initially, researchers used glass shake flasks; however, with the development of disposable technology, more and more scientists have begun using plastic shake flasks, which are roughly the same in shape and structure as glass flasks. While traditional shake flasks dominate the field of bacterial / cell culture, their overly simple structure leads to time-consuming and inefficient operation. Therefore, it is necessary to improve existing shake flasks. Summary of the Invention

[0003] In view of this, the present invention aims to overcome the deficiencies in the prior art and proposes a shake flask for mass production of 3D cell derivatives.

[0004] To achieve the above objectives, the technical solution created by this invention is implemented as follows:

[0005] A shake flask for mass production of 3D cell derivatives includes a base and a main body, wherein the outer diameter of the base is larger than the outer diameter of the main body; the main body is arranged vertically, and its lower end is inserted into the cavity of the base; the upper end of the main body is provided with several protruding rings, both ends of the main body are open structures, the bottom plate of the base is provided with an upward protruding boss, the end of the main body that extends into the cavity of the base is provided with several functional grooves, the lower diameter of the base is smaller than the upper diameter, and the upper part of the base has an arc-shaped structure, with the upper and lower parts transitioning through rounded corners.

[0006] Furthermore, the main body is provided with 2-3 raised rings.

[0007] Furthermore, the functional slot extends to the top plate of the base.

[0008] Furthermore, the functional slot adopts a V-shaped slot or a U-shaped slot.

[0009] Furthermore, the top surface of the boss is a plane.

[0010] Furthermore, the length of the main body extending into the base is 1 / 4 to 1 / 2 of the height of the base.

[0011] Furthermore, the base is arranged coaxially with the main body.

[0012] Furthermore, the diameter of the base is 1.5-3 times the diameter of the main body.

[0013] Compared with existing technologies, the present invention has the following advantages:

[0014] This invention features a rationally designed structure. By placing a base on the lower side of the main body, with a diameter larger than that of the main body and the lower end of the main body extending into the inner cavity of the base, the lower end of the main body acts as a stirrer. This simplifies operation, increases solution mixing efficiency, and reliably ensures the uniformity of solution mixing, which is more conducive to the mass production of 3D cell derivatives. Simultaneously, several raised rings are provided at the upper end of the main body. These rings increase friction, facilitating the clamping and fixing of the shake flask and allowing for the attachment of end caps to the upper end of the shake flask, forming a seal between the end caps and the raised rings. Attached Figure Description

[0015] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0016] Figure 1 is a schematic diagram of the structure created by the present invention;

[0017] Figure 2 is a cross-sectional view of the invention.

[0018] Figure 3 is a schematic diagram of the main body of the present invention;

[0019] Figure 4 is a schematic diagram of the invention having a lower partition protrusion and a side partition protrusion;

[0020] Figure 5 is a schematic diagram of the base part in Figure 4. Detailed Implementation

[0021] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0022] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this invention. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0023] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art will understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0024] The invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0025] A shake flask for mass production of 3D cell derivatives, as shown in Figures 1 to 3, includes a base 1 and a main body 2, the outer diameter of which is larger than that of the main body. The main body is arranged vertically, with its lower end inserted into the cavity of the base. Typically, the base and the main body are arranged coaxially. The upper end of the main body is provided with several raised rings 3. For example, the main body is provided with 2-3 raised rings. The raised rings can increase friction, facilitate the clamping and fixing of the shake flask, and also facilitate the attachment of end caps to the upper end of the shake flask. The end caps and the raised rings form a seal, ensuring the shake flask's airtightness.

[0026] Both ends of the main body are open structures. The base plate 11 of the base has an upwardly protruding boss 4. The end of the main body that extends into the inner cavity of the base has several functional slots 5. The diameter of the lower part 6 of the base is smaller than that of the upper part, and the cross-section of the upper part 10 (side wall) of the base is arc-shaped. The upper and lower parts are transitioned by a rounded corner 7. Typically, the top surface of the boss is flat.

[0027] For example, the functional groove is a V-shaped groove or a U-shaped groove. The functional groove extends to the top plate 12 of the base, facilitating the pouring of the solution inside the base cavity. To facilitate cleaning the shaking flask, a drain port can be provided on the bottom plate of the shaking flask, which is normally sealed with a plug. Since the lower end of the main body extends into the base, when mixing the solution, simply shaking the shaking flask causes the solution to collide with the lower end of the main body, thus enabling the lower end of the main body to act as a stirrer. Furthermore, because the lower end of the main body has a functional groove, the mixing effect is further enhanced as the solution passes through the functional groove; that is, the solid part between each two functional grooves acts as a stirring rod.

[0028] For example, the length of the main body extending into the base is 1 / 4 to 1 / 2 of the base height. The diameter of the base is 1.5 to 3 times the diameter of the main body. This structural design makes the main body easier to hold or clamp, while the base has a larger volume and surface area, facilitating mass production of 3D cell derivatives and improving production efficiency.

[0029] In an optional embodiment, as shown in Figures 4 and 5, the base plate has a lower partition protrusion 8, which is annular, with a boss located at its center. Additionally, a side partition protrusion 9 is provided on the upper part of the base, also annular, surrounding the upper inner wall. In this configuration, with both the lower and side partition protrusions within the base, the solution at the bottom of the base can be thoroughly mixed simultaneously by the lower partition protrusion and the boss when the flask is shaken. Meanwhile, the solution at the top of the base is thoroughly mixed by the side partition protrusion and the end of the main body extending into the base. This effectively improves the efficiency of solution mixing, reliably ensures the uniformity of solution mixing, and is more conducive to the mass production of 3D cell derivatives.

[0030] This invention features a rationally designed structure. By placing a base on the lower side of the main body, with a diameter larger than that of the main body and the lower end of the main body extending into the inner cavity of the base, the lower end of the main body acts as a stirrer. This simplifies operation, increases solution mixing efficiency, and reliably ensures the uniformity of solution mixing, which is more conducive to the mass production of 3D cell derivatives. Simultaneously, several raised rings are provided at the upper end of the main body. These rings increase friction, facilitating the clamping and fixing of the shake flask and allowing for the attachment of end caps to the upper end of the shake flask, forming a seal between the end caps and the raised rings.

[0031] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A shake flask for mass production of 3D cell derivatives, characterized in that: It includes a base and a main body, with the outer diameter of the base being larger than that of the main body. The main body is arranged vertically, with its lower end inserted into the cavity of the base. The upper end of the main body is provided with several protruding rings, and both ends of the main body are open structures. The bottom plate of the base is provided with an upwardly protruding boss. The end of the main body that extends into the cavity of the base is provided with several functional slots. The lower diameter of the base is smaller than the upper diameter, and the upper cross section of the base has an arc-shaped structure, with the upper and lower parts transitioning through rounded corners.

2. The shake flask for mass production of 3D cell derivatives according to claim 1, characterized in that: The main body is provided with 2-3 raised rings.

3. The shake flask for mass production of 3D cell derivatives according to claim 1, characterized in that: The functional slot extends to the top plate of the base.

4. The shake flask for mass production of 3D cell derivatives according to claim 1, characterized in that: The functional slots are either V-shaped or U-shaped.

5. The shake flask for mass production of 3D cell derivatives according to claim 1, characterized in that: The top surface of the boss is a plane.

6. The shake flask for mass production of 3D cell derivatives according to claim 1, characterized in that: The length of the main body extending into the base is 1 / 4 to 1 / 2 of the height of the base.

7. The shake flask for mass production of 3D cell derivatives according to claim 1, characterized in that: The base is arranged coaxially with the main body.

8. The shake flask for mass production of 3D cell derivatives according to claim 1, characterized in that: The diameter of the base is 1.5-3 times the diameter of the main body.

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